Labor productivity exerts a significant influence on the construction cycle and investment in hydropower projects. Consequently, it is a crucial factor in the estimation of the cost of hydropower project construction. The mechanisms that are modulated by subjective factors have been extensively studied. However, the assessment of labor productivity in objective environments with regular changes is usually considered using a single factor, especially in special environments where the influence of environmental factors is of greater concern. As the most extensive region of China’s water-energy resources, the Southwest region has a lengthy tradition of using altitude or oxygen as an inherent criterion for the assessment of labor productivity. However, the applicability of inherent standards is limited. Therefore, we tried to assess the spatial and temporal changes in labor productivity based on the changes in meteorological conditions in Southwest China, employing climate comfort indicators in this study. The result identified five distinct regions of climate change in Southwest China (R-I to R-V). In particular, there is minimal variation in labor productivity as assessed by climate comfort indicators and the elevation between R-I and R-II. R-III and R-IV are influenced by oxygen, temperature, and humidity, while R-V is affected by a multitude of factors. Our findings indicate that temporal and spatial variations in meteorological conditions can result in up to a threefold difference in productivity at the same altitude in different regions. Importantly, our study provides valuable theoretical insights for engineering project management. In particular, it can be concluded that altitude is not a reliable indicator for evaluating labor productivity in high-altitude construction.