Today, magnetic resonance imaging (MRI) is rarely used in managing the care of premature neonates. This is in large part due to the medical and logistical challenges associated with moving neonates from the neonatal intensive care unit (NICU) to the radiology department. Furthermore, acoustic noise associated with MR scanning poses safety concerns for both practitioners and neonatal patients. A small-format 3.0-T neonatal scanner was recently developed and placed within the NICU to address these logistical and acoustic challenges. To compare acoustic noise measurements of a small-format 3.0-T neonatal MRI scanner with conventional adult-sized 1.5-T and 3.0-T MRI scanners using identical neonatal head imaging protocols. Sound pressure level (SPL) measurements of a standard imaging protocol were made in a small-format neonatal 3.0-T MRI scanner as well as in adult-sized 1.5-T and 3.0-T scanners. SPL measurements were made with a Brüel & Kjær sound level meter model 2250. The statistical significance of the differences in SPL between scanners was determined using one-way ANOVA. Average sound pressure level values were measured in unweighted decibels (dB) and A-weighted decibels (dBA) for all imaging sequences in the protocol. The average A-weighted SPLs for the NICU from 1.5-T and 3.0-T MRI scanners were 81.02 ± 0.28 dBA, 87.00 ± 0.85 dBA, and 94.91 ± 0.65 dBA, respectively. SPLs at the isocenter of the NICU MRI scanner were 5.98 dBA quieter than in the 1.5-T scanner (P=0.007), and 13.89 dBA quieter than in the 3.0-T scanner (P<0.001). For staff standing next to the scanner, the NICU scanner was 20.24 dBA quieter than the 1.5-T scanner (P<0.001) and 19.28 dBA quieter than the 3.0-T scanner (P<0.001). The NICU 3.0-T MRI system is significantly quieter than conventional adult-sized MRI systems, improving safety for neonatal patients. Significant reductions in SPL were also noted inside the screen room where clinicians may be present during scanning.