Back ground: Recent studies about plants and botanical components used for different diseases and disorders as well as tradition herbal medicine had become the focus of attention of researchers and specialists in medicine. Objective: The current study aimed to investigate the antiviral activity of star anise seeds and black seeds aqueous extracts against Polio virus in L20B and Rd cell lines culture for understanding herbs extracts antiviral activity towards RNA covid 19 virus. Material and Methods: Applying two methods of in vitro treatment, pre-treatment method and simultaneous treatment either by treating the infected cell with Polio virus suspended in 2 ml culture tubes or treating the infected cells with Polio virus in a microtiter plated of 96 flat bottoms wells.Results: The antiviral activity against Polio virus was improved through simulations treated of infected cells with aqueous extract of star Anise seeds and Black seeds in vitro even the later gave the more potent effect.Conclusion: Different biological activates might be attributed to the differences in the active secondary metabolites constituent in plants extract made estimation for their antiviral activity vary from each other.