Proline Research Articles

Epigallocatechin 3-Gallate as an Inhibitor of Tau Phosphorylation and Aggregation: A Molecular and Structural Insight.

Polyphenols such as Epigallocatechin-3 gallate (EGCG) are currently bearer of hope to prevent or at least to slow down the deleterious effect of Tauopathies such as Alzheimer disease. One of the main effects of these neurodegenerative pathologies is the hyperphosphorylation and consequent aggregation of the Tau protein that leads to the irremediable neuronal cells death. In the present paper, we show how EGCG can play a crucial role to prevent Tau aggregation: (i) in binding Tau in its phosphorylation region with an affinity of the same order of magnitude than kinases (0.5 mM), hindering their access to the protein and (ii) in modifying the 3D-structure of Tau whose preferential conformation changes in the presence of EGCG. For this purpose, two peptides were synthesized, one of 20 residues long issued from the first Proline-rich region of Tau (171Ile-190Lys), the second of 50 residues long (171Ile-220Thr) corresponding to more than 50% of the Tau Proline rich domaine. The total attribution of all the 1H, 13C and 15N resonances of the two peptides has been achieved thanks to a "divide and conquer" strategy leading to their 3D structure preference and their affinity towards EGCG.

Physiological responses of olive cultivars to salinity stress

The aim of this study was to evaluate the tolerance of seven promising olive cultivars for southern parts of Iran (‘Amigdalilolia’, ‘Dakal’, ‘Zard’, ‘Dezful’, ‘Tokhm-e-Kabki’, ‘Shiraz’, and ‘Conservalia’) against salinity stress. Biochemical and physiological responses of the cultivars irrigated with saline water application (control, 4, 8, and 12 dS m-1) were evaluated and the tolerant cultivars were identified. In contrast to the tolerant cultivars, the sensitive ones continue to grow with lower rate and died under salinity stress. In general, growth indices of olive cultivars were reduced with increasing salinity stress and the lowest growth indices were obtained under 12 dS m-1 treatment. Results indicated that the accumulation of higher levels of soluble carbohydrates and proline in the leaves of the tolerant cultivars helps them to deal with salinity stress. The results showed that saline waters up to 4 dS m-1 for irrigation can be used for olive cultivars, however, based on the result of this study, it is not recommended to use water sources with higher electric conductivities to irrigate sensitive olive cultivars. We concluded that the tolerant cultivars stopped growth and used their energy to defend against the salinity stress.

Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseus

ABSTRACTCatharanthus roseus (C. roseus) plants were used to investigate the terpenoid indole alkaloids (TIAs) accumulation under the condition of PEG-induced drought stress. Multivariate analysis showed that 35% PEG6000 could induce more obvious and stable accumulation on proline (PRO) content and the relative water content (RWC). The results indicated that there were gradual increase and then decrease (p < .05) in the contents of vindoline (VIN) and catharanthine (CAT) under 35% PEG6000 stress, but the content of vinblastine (VBL) increased gradually. In addition, the expression levels of tryptophan decarboxylase (TDC), strictosidine synthase (STR) and deacetylvindoline-4-O-acetyltransferase (DAT) were upregulated in plants under 35% PEG6000 stress. Further correlation analysis indicated that CAT accumulation was significantly correlated with TDC gene expression, and VBL accumulation was significantly correlated with peroxidase (p < .05). Our results suggest that the cultivation of C. roseus in drought stress would serve as effective treatment for accumulating TIAs.

HIF-pathway proteins: central regulators of tumor immunology

Low oxygen pressure or hypoxia is often an obstacle in pathological conditions, including during cancer development and metastatic dissemination. The main family of transcription factors involved in the adaptation to inappropriate oxygen levels are the ‘Hypoxia-Inducible Factors’ (HIFs) that together with HIF prolyl hydroxylases (PHDs) regulate many different processes, during physiological as well pathological conditions. Additionally, the entire HIF transcription cascade is involved in the ‘seventh’ hallmark of cancer; tumor promoting inflammation. Previous work revealed that HIF-pathway proteins influence tumor associated immune cells in their action toward proliferation, differentiation, vessel normalization and tumor dissemination. These changes are not necessarily analogous to the role of these proteins in non-tumor related immune responses, where hypoxia often encourages a strong inflammatory response. Recently, Clever et al. demonstrated that T-cell intrinsic inhibition of all three PHDs (PHD1-3) is necessary but sufficient to limit CD4+-regulatory T (T reg ) cell induction and stimulates CD8+ T cell effector function. Interestingly, this resulted in the inhibition of local tolerance in the lung but reduced tumor colonization. In this perspective, we discuss the effect of targeting HIF-pathway proteins in tumor-associated cell lineages, their therapeutic prospective and possible pitfalls.

Physiological Responses To Excess Boron In Wheat Cultivars

This study investigates the response of two wheat cultivars to boron toxicity stress. Plants were cultivated in sand culture and boron was applied to the culture for 10-day. Symptoms, tiller number, boron concentration, soluble sugars, proteins and other free amino acids than proline were studied. The differences between the cultivars were apparent from higher boron and the chlorosis in tolerant cultivar was about 7% compared to the sensitive one 70%. Tiller number gradual decreased in tolerant-cultivar, while in sensitive one a dramatic reduction was exhibited by increasing boron level in culture media. In most boron levels, although the accumulation of soluble carbohydrates was significantly stimulated in shoot of B-sensitive cultivar (Gemmeza 9; S), there were no appreciable differences in the production of carbohydrates in shoot of B-tolerant cultivar (Sakha 93; T). However, the soluble proteins production did not affect by most boron levels in both cultivars. The presence of boron at various concentrations induced a production of free amino acids in shoots of each of the two test cultivars. Tiller number (yield index) decreased in the two test cultivars and was in range 50-59 and 84-92% less than control plants for tolerant and sensitive cultivar, respectively.

Exogenous Proline and Glycinebetaine Mitigate the Detrimental Effect of Salt Stress on Rice Plants

Exogenous application of proline and glycinebetaine (hereafter betaine) is often regarded as a shotgun approach for the protection and survival of plants under abiotic stresses including salinity. Here, we investigated effects of proline and betaine on hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA) and transpiration rate in salt-stressed rice plants. Generally, salt stress increased H 2 O 2 and lipid peroxidation as indicated by MDA content and decreased transpiration rate in rice plants. The exogenous application of proline and betaine decrease H 2 O 2 and MDA contents and increase transpiration rate in salt-stressed rice plants. It is suggested that exogenous proline and betaine mitigate the detrimental effects of salt stress by reducing H 2 O 2 and lipid peroxidation levels and by increasing transpiration rate in rice plants.

Responses of Physiological Indices of Typical Submerged Macrophytes to Water Quality in Taihu Lake

It was well known that physiological indices of submerged macrophytes could reflect change of water quality. The correlation between physiological indices of submerged macrophytes and change of water quality was studied under the cooperation of in-situ monitoring and lab analysis, combined with measuring Chlorophyll and free proline (PRO) contents as well as peroxidase (POD) activities in the leaves of Potamogeton wrightii Morong and Potamogeton crispus L. under different water quality and nutrition status. The results showed: ①there were significant spatial differences among water factors and the comprehensive eutrophication index (TLI) of distribution areas of Potamogeton wrightii Morong and Potamogeton crispus L. Mesotrophic water was more suitable for the growth of Potamogeton wrightii Morong, while Potamogeton crispus L. grew well in eutrophic water. ②there were significant spatial differences among physiological indices of Potamogeton wrightii Morong and Potamogeton crispus L. in Taihu Lake. Besides, there were significant relationships between Chlorophyll contents, POD activities of two species and TLI. ③water transparency as well as nitrogen and phosphorus nutrition were important factors leading to changes in Chlorophyll contents and POD activities of Potamogeton wrightii Morong and Potamogeton crispus L.. The findings from this study indicate that physiological properties of Potamogeton wrightii Morong and Potamogeton crispus L. have a very close correlation with nutrition status and physiochemical properties of water.

Defensive response of radish seedlings to the oxidative stress arising from phenolic compounds in the extract of peppermint (Mentha × piperita L.)

Understanding the mode of action of allelochemicals, as the cause of oxidative stress and induction of antioxidant mechanism, is one of the challenging aspects in allelopathic studies. Accordingly, the aim of the present study was to analyze the effect of allelochemical stress, induced by peppermint water extract (WE), on radish (Raphanus sativus L.) growth. The peppermint WE concentrations were 0, 2, 4, 6, 8, and 10% (v/v). Some phenolic compounds of the peppermint WE determined by HPLC were trans-ferulic acid (10.8mg/g), hesperidin (9.3mg/g), ellagic acid (6.8mg/g), and sinapic acid (4.2mg/g). The maximum inhibitory effect of peppermint extract on germination and growth was obtained at 8% WE concentration. The results showed that the compounds in the extract of peppermint had significant effect on the amount of proline (PRO), soluble sugars and total phenols, as well as on the activity of antioxidant enzymes, and relative membrane permeability (RMP) (P<0.05). However, with the exception of super oxide dismutase (SOD), all of the treatments had a significant (P<0.05) positive effect on the activity of other enzymes such as ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POD). The results suggest that oxidative stress caused by high levels of reactive oxygen species (ROS) resulting from peppermint allelochemicals inhibited the growth of the radish seedlings, and their ability to resist the phytotoxic allelochemicals of peppermint, which is associated with the activity of protective enzymes and the defensive function of radish plants.

Plasma free amino acid profile changes in hepatocellular carcinoma patients

Objective The aim of this study was to investigate plasma amino acid profile as a possible diagnostic/prognostic method in patients with hepatocellular carcinoma. Background Many previous reports have shown that metabolism is notably altered in cancer cells. Thus, various methods for cancer diagnosis and prognosis have been developed based on metabolite analysis. Among whole metabolites, amino acids were proposed as suitable candidates for focused metabolomics as they are either ingested or synthesized endogenously and they play an essential physiological role both as basic metabolites and metabolic regulators. Patients and methods This study included 71 hepatocellular carcinoma patients (60 male and 11 female) and 30 chronic hepatitis C virus patients (20 male and 10 female) in addition to 30 healthy controls (25 male and 5 female). Laboratory investigations including complete blood picture, liver function tests, serum α-fetoprotein, hepatitis viral markers (HBsAg and anti-HCVAb) were carried out for all participants. Amino acid assay was carried out using ultra performance liquid chromatography–electrospray ionization-mass spectrometry. Results The study showed a statistically significant increase in methionine, tyrosine, ornithine, citrulline, glycine, phenylalanine, alanine, glutamate, proline, and arginine and a decrease in valine, aspartate, leucine/isoleucine, branched tyrosine ratio (BTR), and Fischer's ratio in hepatocellular carcinoma compared with the control group. Moreover, the study indicated that there was a statistically significant correlation between BTR and platelets, alanine transaminase, albumin, bilirubin, and α-fetoprotein and significant differences in BTR among Child-Pugh A, B, and C. Conclusion Our results suggested that the plasma free amino acid profile is considered valuable for diagnosis and nutritional care in cancer patients. Furthermore, BTR reflects the pathological liver background with a high degree of correlation with other liver functional markers.

Genomic analysis of missense and silent mutations in CatSper 2 gene in infertile male cases.

Background: Infertility is described as inability of a couple to conceive after a period of one year regular unprotected intercourse. About 10% - 15% of couples experience some form of infertility. Male infertility is a multifactorial disease process with a number of potential contributing causes; several factors may contribute to its etiology. Cation channels of sperm is a small family of ion channels, normally referred to as CatSper channels (CatSper1, 2, 3 & 4) which are putative six-transmembrane (6TM) spanning proteins and seem to be specific to sperm cells. CatSper 2 gene involved in sperm motility and hyper activation. Materials and methods: We performed mutational analysis of the CatSper2 gene in 100 infertile males and 100 healthy controls. To screen mutations inCatSper2 gene, we performed PCR followed bydirect DNA sequencing. Results: Analysis of mutational screening of CatSper2 gene, we found a single nucleotide change (CT G →CT A ) in five asthenozoospermia at exon 3 resulting in silent mutation and (C G T→C C T)in eight asthenozoospermia at exon 6 missense mutation as the change leads to amino acid sequence change from Arginine to Proline. Conclusions: On mutational screening of CatSper2 gene, at exon 6 there was missense mutation that leads to conformational structural change inspite of the amino acids being hydrophobic and non-polar in nature. The identified mutations may be a cause of asthenozoospermia in humans.

Backbone and Partial Side-Chain Chemical Shift Assignments and DynamicsMeasurements for The Catalytic Domain of Human Prolyl Hydroxylase Domain 2 (PHD2) With Zn(II), 2-Oxoglutarate (2OG) and Hypoxia Inducible Factor-alpha(HIF-alpha) Peptide

Backbone and Partial Side-Chain Chemical Shift Assignments and DynamicsMeasurements for The Catalytic Domain of Human Prolyl Hydroxylase Domain 2 (PHD2) With Zn(II), 2-Oxoglutarate (2OG) and Hypoxia Inducible Factor-alpha(HIF-alpha) Peptide

Backbone and Partial Side-Chain Chemical Shift Assignments and DynamicsMeasurements for The Catalytic Domain of Human Prolyl Hydroxylase Domain 2 (PHD2) With Zn(II) and 2-Oxoglutarate (2OG)

Backbone and Partial Side-Chain Chemical Shift Assignments and DynamicsMeasurements for The Catalytic Domain of Human Prolyl Hydroxylase Domain 2 (PHD2) With Zn(II) and 2-Oxoglutarate (2OG)

Serum metabolite changes of aged rats with post-anesthesia cognitive dysfunction

Objective To explore the effects of isoflurane on blood plasma metabolites (BPM) and its correlation with cognitive dysfunction. Methods Thirty female Sprague-Dawley (SD) rats were randomly divided into two groups: rats in the control group (n=10) received 80% oxygen for 2 hours (h); and rats in the isoflurane-treated group (n=20) were anesthetized with isoflurane and 80% oxygen for 2 h. Cognitive functions were examined using a Y-maze test to explore the learning times of rats. The level of blood plasma metabolites was detected through gas chromatography-mass spectrometry (GCMS). Results The learning times of rats in the isoflurane-treated group was more than the learning times of rats in the control group [(70.75±15.30) vs (45.40±11.21), P<0.05]. D-fructose, arabinofuranose, n-butylamine, and inositol significantly increased (P<0.05), respectively. Whereas, L-analine and L-proline significantly decreased (P<0.05) in isoflurane-treated rats when compared to those in the control rats (P<0.05). Moreover, plasma concentrations of d-fructose, arabinofuranose, n-butylamine, inositol, and L-proline were positively correlated with the degree of cognitive dysfunction in the isoflurane-treated rats. Conclusions Changes of plasma concentrations of inositol, and d-fructose in aged rats are useful in predicting the occurrence and progression of post-anesthesia cognitive dysfunction. Key words: Anesthesia/AE; Cognition disorders/ME; Isoflurane/AE

Responses of chilling resistance to photoperiodic dormancy induction in peach leaf.

Taking 6-year-old "Chunjie" peach as test material, and the peach in natural condition as the control, this paper investigated induced effects of long-day and short-day photoperiod on dormancy and responses of chilling resistance to photoperiodic induction during dormancy induction process. The results showed that the trees of long-day and short-day treatments could both enter dormancy induction under the gradually decreasing temperature. The long-day treatment was 1 week later than the control, while the short-day treatment was 1 week earlier. The total water content and free water content both decreased, and the bound water content and the ratio of bound water/total water increased with the development of dormancy. SOD and CAT activities changed as unimodal curve during dormancy induction, and the peak values appeared at the late stage of dormancy induction, POD activity decreased rapidly after the start of dormancy induction, and rebounded to form a small peak at the late stage of dormancy induction. The soluble protein content declined, proline and malonaldehyde (MDA) increased continuously, and the injury rate increased. Long-day could increase SOD and CAT activities and proline content, alleviate the decline of POD activity and soluble protein content, and reduce the growth rate of MDA and injury rate, which indicated leaf damage was lighter in long-day treatment than in the control. However, they changed differently under short-day treatment, especially the leaf injury rate was higher than the control, exhibiting a lower chilling resistance. Prolonging illumination was suggested to improve leaf chilling resistance in practical production if environmental temperature permitted.

Secondary metabolites of peppermint change the morphophysiological and biochemical characteristics of tomato

Abstract Germination and growth of plants are influenced by allelochemicals that mostly cause crops , yield reduction. In the present study, the effect of stress arising from allelopathic compounds in the water extract (WE) of peppermint ( Mentha×piperita L. CV. Mitcham) on the morphophysiological and biochemical characteristics of tomato ( Lycopersicon esculentum Mill. CV. Rio Grande) was investigated. Different concentrations (0, 2, 4, 6, 8, and 10% (v/v)) of the WE were examined. Some phenolic compounds of the WE determined by the HPLC instrument were trans-ferulic acid (10.8 mg/g), hesperidin (9.3 mg/g), ellagic acid (6.8 mg/g), and sinapic acid (4.2 mg/g). The results showed that the maximum inhibitory effect on germination and growth (dry weight, and leaf area) was obtained at the concentration of 10% (v/v) extract, and its compounds had significant effect on the amount of proline (PRO), soluble sugar and starch, as well as on the activities of tomato,s antioxidant enzymes such as ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) at the 5% level. None of the treatments had a significant effect on the SPAD chlorophyll meter reading of tomato plants. It could be stated that the compounds present in the extract of peppermint must lead to high levels of reactive oxygen species (ROS), and subsequent oxidative stress inhibits the growth of the seedlings; however, more research is still required in this regard.

Biomimetic hydrogel loaded with silk and l-proline for tissue engineering and wound healing applications.

The aim of this article was to develop silk protein (SF) and l-proline (LP) loaded chitosan-(CS) based hydrogels via physical cross linking for tissue engineering and wound healing applications. Silk fibroin, a biodegradable and biocompatible protein, and l-proline, an important imino acid that is required for collagen synthesis, were added to chitosan to improve the wound healing properties of the hydrogel. Characterization of these hydrogels revealed that CS/SF/LP hydrogels were blended properly and LP incorporated hydrogels showed excellent thermal stability and good surface morphology. Swelling study showed the water holding efficiency of the hydrogels to provide enough moisture at the wound surface. In vitro biodegradation results demonstrated that the hydrogels had good degradation rate in PBS with lysozyme. LP loaded hydrogels showed approximately a twofold increase in antioxidant activity. In vitro cytocompatibility studies using NIH 3T3 L1 cells showed increased cell viability (p < 0.01), migration, proliferation and wound healing activity (p < 0.001) in LP loaded hydrogels compared to CS and CS/SF hydrogels. Cell adhesion on SF and LP hydrogels were observed using SEM and compared to CS hydrogel. LP incorporation showed 74-78% of wound closure compared to 35% for CS/SF and 3% for CS hydrogels at 48 h. These results suggest that incorporation of LP can significantly accelerate wound healing process compared to pure CS and SF-loaded CS hydrogels. Hence, CS/LP hydrogels could be a potential wound dressing material for the enhanced wound tissue regeneration and repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1401-1408, 2017.

Metabolomics study on osteonecrosis of femoral head based on UPLC-MS/MS

Objective To investigate the metabolism characteristics and the potetial biomarker candidates of osteonecrosis of the femoral head (ONFH) using metabolomic technology. Methods The femoral head specimens from 23 ONFH patients (25 necrotic femoral heads) and 18 normal femoral heads from femoral neck fracture patients were collected for histopathological examination to confirm the diagnosis of all samples. All the metabolites of bone trabecula were extracted for ultrahigh performance liquid chromatography-MS/MS analyzed. The measured variables was pretreat, and PCA (principal component analysis), PLS-DA (partial least squares-discriminant analysis) and OPLS-DA (orthogonal-partial least squares-discriminant analysis) models were employed to confirm the difference between these two groups after UPLC-MS/MS (ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry) analysis. At last, the differential variables were screened out by PLS-DA and variate analysis (Kruskal-Wallis H test). The changed metabolites were confirmed by MS and MS/MS aligned in HMDB (human metabolomic database) and Massbank. The changed metabolites with the most obviously changed peak abundance, D-arginine, L-proline and L-glutamine, were picked out as the potential diagnostic biomarkers. After binary logistic regression analysis, the combined biomarkers candidates were further analyzed by receiver operating characteristic (ROC) curve to evaluate the significance of the combined biomarkers. Results Significant distinction of metabolites expression mode can be seen in PCA, PLS-DA and OPLSDA models scoring plots between ONFH and control groups. Twelve changed metabolites in ONFH bone trabeculas were confirmed by multi-variate statistical analysis and variate statistical analysis. Compared with the femoral neck fracture patients, the increased metabolites included D-arginine, L-proline, L-glutamine, creatine, uracil, uridine, LysoPC(20∶4(5Z, 8Z, 11Z, 14Z)), LysoPC(16∶0), PC(20∶1(11Z)/18∶3(6Z, 9Z, 12Z)) and PE(P-16∶0e/0∶0). The decreased metabolites were reticulataxanthin and β-cryptoxanthin. According to the change fold of peak abundance and variable weight projection in PLS-DA, the most obviously differential metabolites were picked out as the biomarker candidates of ONFH. The potential biomarkers candidates were identified as D-arginine, L-proline and L-glutamine. The area under the curve of D-arginine, L-proline and L-glutamine ROC were 0.873, 0.712 and 0.862. The area under the curve of ROC was 0.946 after combining D-arginine, L-proline, L-glutamine using binary logistic regression analysis. Conclusion PCA, PLS-DA and OPLS-DA models were used to find out the differential variables in the metabolites of bone trabeculas in ONFH and femoral neck fracture patients. Twelve metabolites were identified by MS/MS, and 3 obviously changed metabolites, D-arginine, L-proline, L-glutamine, were indicated as biomarker candidates. These 3 obviously changed metabolites showed a good diagnostic significance. Key words: Femur head necrosis; Mass spectrometry; Metabolomics; Biological markers

Clavulanic acid separation on fixed bed columns of layered double hydroxides: Optimization of operating parameters using breakthrough curves

The adsorption of clavulanic acid (CA) in a fixed-bed column of layered double hydroxides (LDHME) was investigated. Breakthrough curves were obtained experimentally and the system was evaluated with regards to column operation time, efficiency and productivity as functions of simultaneous variations of superficial velocity (vz) and bed height (L) using a central composite rotatable design (CCRD). At the optimized condition (vz=1.00cm/min and L=6.5cm), the responses were: 46min, 146min, 100min and 13kg/hcm3, for breakthrough time (tb), exhaustion time (te), the difference te−tb and productivity (P), respectively. These results represent no change in tb, but a 50% decrease of te, 40% decrease of (te−tb) and 38% increase of P, which is advantageous for the process. The factorial design technique was shown to be an efficient tool for assessing factor influences and was effective in optimizing the column operating conditions. Good separation of CA from the amino acids tyrosine (TYR) and proline (PRO) was observed in fixed bed columns. The LDHME adsorbent was shown to be an excellent alternative for CA purification.

Effects on antioxidant enzyme activities and osmolytes in Halocnemum strobilaceum under salt stress

The seedlings of Halocnermum strobilaceum were cultivated in 0.5% hoagland nutrient solution containing 0.0%, 0.9%, 2.7% and 5.4% of NaCl as well as composite salt (Na+, Ca2+, K+, Si4+) for 20 days; all the contents are in weight ratio. Succulent level, inorganic ions (Na+, K+), organics such as betaine, proline, malondialdehyde, and antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), betaine aldehyde dehydrogenase (BADH) were measured to reveal its salt tolerance mechanism. When the composite salt concentration reaches 5.4%, SOD activity level, and MDA content is five times the control group; when it reaches 2.7%, the succulent level of seedlings, and the content of K+ in roots is nearly two times the NaCl treatment; the dry weight is more than three times the control group; with the NaCl treatment, MDA is three times the contrast; when the salt concentration is 2.7%, POD reaches the maximum. Results indicate that Si4+, K+, and Ca2+ from composite salt in the roots of H. strobilaceum improved the water-holding capacity. The activities of antioxidant enzyme were raised by the accumulation of proline and betaine, which increased the salt tolerance. The absorption of K+ promoted the high ratio of K+/Na+ and alleviated the damage of cell membranes of H. strobilaceum, which is associated with osmotic contents such as betaine and proline.

Evaluation of Molecular Mapping Population from Wheat/Synthetic Hexaploid Cross for Drought Tolerance

To enhance the breeding efficiency in stress prone environments globally several molecular mapping populations have been produced. The focus of current study was to evaluate the mapping population derived from the cross of drought susceptible OPATA and drought tolerant synthetic hexaploid wheat. After field screening the selected genotypes were evaluated under in vitro conditions for physiological parameters like proline accumulation, chlorophyll content and SOD activity. Although, physiological evaluation has marked all these genotypes as drought tolerant but the performance of lines lines L-14, L-17, L-23, L-55, L-87, L-80, L-78, L-108, L-118, L-122, L-150 and L-152 was notable owe to their better physiological defense mechanism to drought. The present study concluded that unique genetic diversity from Aegilops tauschii (D genome donor) can be harnessed to get more yield by improving the existing cultivars against abiotic stress.