Megacopta Cribraria Research Articles

Ecological Insights and First Confirmed Record of Ooencyrtus nezarae (Hymenoptera: Encyrtidae) on Eggs of Megacopta cribraria (Hemiptera: Plataspidae) in Tennessee and Georgia1

Abstract The kudzu bug, Megacopta cribraria (F.) (Heteroptera: Plataspidae), is native to Asia, was first detected in Georgia, USA, in 2009, and has since been recognized as a damaging pest of soybean (Glycine max (L.) Merrill; Fabales: Fabaceae) in several southeastern states. Megacopta cribraria dispersed rapidly from 2009 to 2013, after which its spread rate declined sharply. Despite this decline, established populations have remained stable. This decline may be partially attributed to natural enemies of kudzu bug. For example, the exotic egg parasitoids Ooencyrtus nezarae Ishii (Hymenoptera: Encyrtidae) and Paratelenomus saccharalis (Dodd) (Hymenoptera: Platygastridae) have been occasionally detected in the United States since 2016 and 2013, respectively. We recovered O. nezarae from M. cribraria eggs collected from wild patches of kudzu (Pueraria montana Lour. (Merrill) var. lobata (Willd.)) throughout summer 2017. Although the occurrence of O. nezarae in southern Georgia has been suggested based on exit holes from kudzu bug eggs, to our knowledge, this report is the first to document and confirm recovery of O. nezarae from kudzu bug eggs in both Tennessee and Georgia. In addition, at the time of collection in 2017, this recovery was the first confirmation of this species from kudzu in North America. This early-season natural enemy combined with the later-occurring entomopathogen Beauveria bassiana (Balsamo-Crivelli) Vuillemin may reduce and maintain kudzu bug densities, which could lessen economic impacts on soybean producers.

Agricultural issues with climate change—case studies with 3 soybean pests: Johnsongrass, kudzu bug, and charcoal rot

Abstract Agricultural production and crop yields are threatened around the world by the emergence and spread of agronomical pests, including diseases, insects, and weeds. Due to changes in precipitation, carbon dioxide levels, and warming temperatures being experienced throughout most of the world, new challenges are emerging for pest management in virtually all major cropping systems. While precise environmental impacts due to climate change are impossible to predict, they will require innovative and new solutions for pest management. In this article, we explore the challenges of 3 problem pest species with soybean: Johnsongrass, kudzu bug, and charcoal rot. Understanding pest responses to climate change is vital for better understanding the new agricultural innovations that will be required to manage them in the future.

Effects of Food Source Availability, Host Egg:Parasitoid Ratios, and Host Exposure Times on the Developmental Biology of Megacopta cribraria Egg Parasitoids.

Parasitoids forage for hosts in dynamic ecosystems and generally have a short period of time to access hosts. The current study examined the optimal reproductive attributes of two egg parasitoids, Paratelenomus saccharalis Dodd (Hymenoptera: Platygastridae) and Ooencyrtus nezarae Ishii (Hymenoptera: Encyrtidae), of the kudzu bug, Megacopta cribraria Fabricius (Hemiptera: Plataspidae). The proportion of O. nezarae and P. saccharalis adult offspring that emerged from M. cribraria eggs and the sex ratio of the parasitoid offspring were compared among treatments for the effects of different adult parasitoid food sources, host egg-to-adult parasitoid ratios, and host exposure times. Our results suggest that honey solution as a food source, a 21:7 host-to-parasitoid ratio, and three-to-five days of exposure time optimized the production of female O. nezarae offspring. For P. saccharalis, honey solution as a food source, a 21:7 host-to-parasitoid ratio, and one day were optimal for maximizing female offspring production. These findings provide new information about the biology of these egg parasitoids.

Chromosome-level genome of the bean bug Megacopta cribraria in native range, provides insights into adaptation and pest management

Megacopta cribraria, a bean pest causing tremendous economic losses in Asia, was discovered in North America in 2009. Although M. cribraria has become the focus of research on biological invasion and pest management, the lack of genomic resources limits in-depth studies. Here, we report the first chromosome-level genome of M. cribraria using Illumina, PacBio, and Hi-C data. The assembled genome size was 699.65 Mb, with a contig N50 of 1.43 Mb and a scaffold N50 of 109.27 Mb. >97.51 % of bases were successfully anchored to six chromosomes. Through genome annotation, a total of 13,308 coding genes were predicted, 96.3 % of which were successfully accessed function. Expanded gene families were involved in proteolysis, protein metabolism and nitrogen metabolism reflected the underlying genome basis for host adaptation during evolution. Transcriptome analysis revealed different gene expression patterns in antenna, mouthpart, head, leg, wing, and carcass body of the adult M. cribraria, respectively. Moreover, the expression profiles of the odorant receptor genes indicated the potential target genes for pest control. The high-quality chromosome-level genome will benefit further research on the adaptation, evolution, and population genetics of the M. cribraria that will assist in the pest management and tracking the biological invasion routes.

Abundance of Halyomorpha halys (Hemiptera: Pentatomidae) and Megacopta cribraria (Hemiptera: Plataspidae) in Soybean in Areas with Few Previous Sightings in Tennessee.

Halyomorpha halys (Stål) and Megacopta cribraria (Fabricius) are two exotic invasive pests that have invaded the United States in recent years. Halyomorpha halys can damage various fruits, vegetables, and field crops, such as soybean and corn, while Megacopta cribraria only attacks soybean and kudzu, a weed species. They are currently found in southeastern states and threaten soybean and other crops grown in the region. This study evaluated the seasonal abundance of H. halys and M. cribraria in soybeans in 2016 and 2017 in two counties in the central region of Tennessee, where both species had either a few sightings or none that were recorded when this research was being planned. Lures and sweep sampling were used to monitor H. halys, and sweep sampling was used to monitor M. cribraria. Halyomorpha halys was first detected in samples in late July. Their numbers increased in early to mid-September, reached the economic threshold in late Sept, and then started to decline. Megacopta cribraria was first detected in mid to late July, increased their populations in September, but did not reach the economic threshold and declined mid-October. Our results showed the seasonal abundances of H. halys and M. cribraria and their establishment in the central region of Tennessee.

Population Dynamics of Insect Pests and Beneficials on Different Snap Bean Cultivars

Snap bean is an important crop in the United States. Insecticides are commonly used against pests on snap bean, but many pests have developed resistance to the insecticides and beneficials are threatened by the insecticides. Therefore, host plant resistance is a sustainable alternative. Population dynamics of insect pests and beneficials were assessed on 24 snap bean cultivars every week for six weeks. The lowest number of sweetpotato whitefly (Bemisia tabaci) eggs was observed on cultivar 'Jade', and the fewest nymphs were found on cultivars 'Gold Mine', 'Golden Rod', 'Long Tendergreen', and 'Royal Burgundy'. The numbers of potato leafhopper (Empoasca fabae) and tarnished plant bug (Lygus lineolaris) adults were the lowest on cultivars 'Greencrop' and 'PV-857'. The highest numbers of adults were found in Week 1 (25 days following plant emergence) for B. tabaci and Mexican bean beetle (Epilachna varivestis); Week 3 for cucumber beetle, kudzu bug (Megacopta cribraria), and E. fabae; Weeks 3 and 4 for thrips; Week 4 for L. lineolaris; and Weeks 5 and 6 for bees. Temperature and relative humidity correlated with B. tabaci, E. varivestis, bee, and predator ladybird beetle populations. These results provide valuable information on the integrated pest management of snap beans.

Direct and Indirect Competitive Interactions between Ooencyrtus nezarae and Paratelenomus saccharalis Parasitizing Megacopta cribraria Egg Patches.

The present study investigated egg parasitoid interspecific interactions between a generalist, Ooencyrtus nezarae Ishii (Hymenoptera: Encyrtidae) and a specialist, Paratelenomus saccharalis Dodd (Hymenoptera: Platygastridae) in a laboratory setting using kudzu bug (Megacopta cribraria Fabricius, (Hemiptera: Plataspidae)) eggs as their shared host. Three experiments were conducted to evaluate the emergence of wasps from parasitized hosts after the simultaneous and sequential release of wasps, monitor aggressive behavior of P. saccharalis, and quantify intraguild predation of O. nezarae larvae on heterospecific P. saccharalis larvae. Results showed that total host egg parasitism was higher when both wasps were released simultaneously than if wasps were released sequentially. Ooencyrtus nezarae produced more total offspring than P. saccharalis in all sequential/simultaneous treatments but produced male offspring in most cases. In the aggressive behavioral experiment, specialist, P. saccharalis used head butting to fight O. nezarae, but no other aggressions were observed. In an experiment examining intraguild predation, O. nezarae was able to develop in host eggs parasitized by P. saccharalis four days earlier, acting as a superior larval competitor. These findings shed light on the potential interspecific interactions between O. nezarae and P. saccharalis, which may determine their relative abundance and influence their compatibility in kudzu bug biological control programs.

Population Dynamics and Parasitism of the Kudzu Bug, Megacopta cribraria, by Egg Parasitoid, Paratelenomus saccharalis, in Southeastern USA

Megacopta cribraria (Fabricius) (Hemiptera: Plataspidae), commonly called the kudzu bug, is a pest of concern in many soybean and legume-producing states. It was first detected in the United States in 2009. In the southeastern United States, M. cribraria reduced crop yields by 47% in untreated soybean fields. Paratelenomus saccharalis (Dodd) (Hymenoptera: Platygastridae) is a known parasitoid of the kudzu bug, and a potential biological control agent. This study was comprised of three phases: (1) Preliminary assessment of the presence of the kudzu bug and its parasites in north Florida and south Georgia. (2) Measurements of the levels of parasitism in 12 sites selected from the preliminary evaluation to compare population dynamics in two growing seasons in agricultural, forested, and urban areas. (3) Laboratory studies to measure parasitism after 21 days in controlled environments. The preliminary assessment showed that in both 2016 and 2017, P. saccharalis emerged from eggs of M. cribraria collected in Leon and Gadsden County. Additionally, parasitism was recorded for the recently discovered egg parasitoid in north Florida, Ooencyrtus nezarae (Ishii) (Hymenoptera: Encyrtidae). In the assessment of parasitism in the field, differences were observed in the average level of parasitism within the urban and forest area in both years, and in the agricultural area for 2017. Between-group comparisons indicated significant differences between the average parasitism levels in agricultural, urban, and forested areas in 2016; in 2017, the differences between the areas were not significant. In the laboratory, after 21 days of observation, an average of 77.4% of freshly laid and 61.6% of cooled eggs were parasitized by P. saccharalis, and adult parasitoids emerged. There was a significant difference in the emergence of freshly laid eggs over cooled eggs. This study suggests that both P. saccharalis and O. nezarae exert some levels of natural control of the kudzu bug in the field and may be useful as biological control agents in an integrated pest management program.

Kudzu Bug (Megacopta cribraria) and Associated Egg Parasitoids Emergence Rates in Alabama are Predicted by Weather Indices.

The kudzu bug, Megacopta cribraria (Fabricius) (Hemiptera: Plataspidae), is a major economic pest of soybean in the southeastern United States. With climate warming, this pest is expected to move northward and cause additional crop damage. Parasitoid biocontrol is a potential method of integrated pest management for kudzu bug. Two species of egg parasitoid wasps have been observed emerging from kudzu bug egg masses in the southeastern United States: Paratelenomus saccharalis (Dodd) (Hymenoptera: Platygastridae) and Ooencyrtus nezarae (Ishii) (Hymenoptera: Encyrtidae). This paper used egg mass emergence data collected between 2018 and 2020 in Alabama soybean fields and compared the data to weather indices. Indices included the number of days with minimum temperatures below zero, accumulated rainfall (mm m-2), as well as species specific metrics of accumulated growing degree days, accumulated daily minimum temperature (°C), and accumulated daily maximum temperature (°C). Emergence of the generalist parasitoid, O. nezarae, was highly correlated with kudzu bug nymph abundance, accumulated degree day, accumulated daily temperatures, and precipitation. Ooencyrtus nezarae emergence was predicted in a stepwise regression equation by aggregated degree day and date of collection, which indicates that seasonality may be a predictor of its presence. In contrast, collections of the specialist parasitoid, P. saccharalis, were near-zero throughout the collection period, suggesting that this species may no longer be a usable biocontrol agent in the southeastern United States as a result of external limiting factors.

Susceptibility of Different Life Stages of Kudzu Bug Megacopta cribraria (F.) (Hemiptera: Plataspidae) to Two Different Native Strains of Beauveria bassiana.

This is the first study that examined and compared the survival, LC50, and RR50 estimates of Megacopta cribraria F. (Hemiptera: Plataspidae) nymphs and adults that were exposed to two native Beauveria bassiana isolates (Previously codified as NI8 and KUDSC strains) at four concentrations. The greatest reduction in survival and mortality was observed primarily on or after 10 d post-exposure to B. bassiana isolates. Survival of early instars (2nd, 3rd) were not affected by either strains or concentration at 3 d and 5 d post-exposure. Survival of later instars (5th) and adults was significantly reduced when exposed to the KUDSC strain at all concentrations. Comparison of dose–mortality values (LC50) using resistance ratios (RR50) were significantly different between life stages of the kudzu bug for both strains of B. bassiana. The LC50 values showed that kudzu bug adults are more susceptible than any other life stage when exposed to either strain. The KUDSC strain was more pathogenic than NI8 10 d after exposure, but NI8 exhibited significantly higher pathogenicity than KUDSC 20 d after exposure. Our results suggest potential field application of B. bassiana for kudzu bug control and their integration into pest management strategies to suppress them before they cause economic damage to soybean crops.

Species boundary and phylogeographical pattern provide new insights into the management efforts of Megacopta cribraria (Hemiptera: Plataspidae), a bean bug invading North America.

Correct identification together with information on distribution range, geographical origin and evolutionary history are the necessary basis for the management and control of invasive species. The bean bug Megacopta cribraria is a crucial agricultural pest of soybean. Recently, M. cribraria has invaded the United States and spread rapidly, causing severe reductions in soybean yields. However, the species boundary and phylogeographical pattern of this invasive bean bug are still unclear. The results of different species delimitation methods (Automatic Barcode Gap Discovery, Assemble Species by Automatic Partitioning, Bayesian Poisson Tree Processes and Bayesian Phylogenetics and Phylogeography) strongly demonstrated that M. cribraria and Megacopta punctatissima represent the same species. M. punctatissima should not be considered a distinct species but rather a variety of M. cribraria. Phylogenetic analyses revealed three well-supported clades (Southeast Asia [SEA], East Asia continent [EAC] and Japan [JA]) with distinct geographical structures in the M. cribraria-M. punctatissima complex. The SEA clade was at the base of the phylogenetic tree, and the sister relationship between the EAC clade and JA clade was strongly supported. The split between the EAC clade and JA clade occurred at approximately 0.71 Ma, corresponding to the submergence period of the East China Sea land bridge. This study clarified the species boundary between M. cribraria and its closely related species and revealed the phylogeographical pattern and evolutionary history of M. cribraria. The species delimitation and phylogeography results achieved in this study could provide new insights into the monitoring and management of this agricultural pest. © 2022 Society of Chemical Industry.

Use of Pyriproxyfen to Induce Oogenesis in Diapausing Megacopta cribraria (Heteroptera: Plataspidae), and Evaluation of Pyriproxyfen-Induced Eggs for Rearing the Parasitoid Paratelenomus saccharalis (Hymenoptera: Scelionidae).

Simple SummaryRearing of many hymenopteran egg parasitoids requires a reliable supply of host eggs. The parasitoid Paratelenomus saccharalis can be reared on eggs produced from field collected kudzu bug, Megacopta cribraria, however field-collected hosts obtained during reproductive diapause do not readily produce eggs and must be reared under a long day-length photoperiod to terminate diapause. In this study we found that an exogenous application of pyriproxyfen was able to terminate diapause, leading to a significant increase in egg production. The eggs produced by pyriproxyfen-treated M. cribraria were accepted by the egg parasitoid Paratelenomus saccharalis, however parasitoid emergence was reduced when compared to eggs from untreated hosts. When the effects of pyriproxyfen treatment on egg production and parasitoid emergence were evaluated together, the net increase in parasitoid yield due to pyriproxyfen treatment was approximately 87%. This method has the potential to increase parasitoid yield and reduce production costs in egg parasitoid rearing programs.The mass rearing of hymenopteran egg parasitoids requires an abundant supply of host eggs. The onset of reproductive diapause and subsequent decline in egg production poses a challenge for parasitoid rearing when using host colonies augmented by field-collected insects. We investigated the application of pyriproxyfen, a juvenile hormone analog, to induce oviposition in diapausing adult kudzu bugs, Megacopta cribraria (Fabricius) (Heteroptera: Plataspidae), and the use of eggs produced by pyriproxyfen-treated kudzu bugs to rear the egg parasitoid, Paratelenomus saccharalis (Dodd) (Hymenoptera: Scelionidae). The effects of pyriproxyfen and photoperiod treatments on host mortality, egg production, and rates of parasitoid eclosion from the eggs were used to calculate the parasitoid yield for the different treatment regimes. A combination of pyriproxyfen and a long-day photoperiod increased the parasitoid yield by 87% compared to acetone and a long-day photoperiod. The general applicability of JH-analog mediated egg production for parasitoid rearing is discussed.

Biopesticide and Parasitoid Effects on Megacopta cribraria (Hemiptera: Plataspidae) Life Stage Density and Egg Parasitism in Soybean

Biopesticide and Parasitoid Effects on <i>Megacopta cribraria</i> (Hemiptera: Plataspidae) Life Stage Density and Egg Parasitism in Soybean

Associating Site Characteristics With Distributions of Pestiferous and Predaceous Arthropods in Soybean.

Although site-specific pest management has the potential to decrease control costs and environmental impact associated with traditional pest management tactics, the success of these programs relies on the accurate characterization of arthropod distributions within a crop. Because potential correlation of insect counts with remotely sensed field attribute data could help to decrease the costs associated with and need for fine-scale spatial sampling, we chose to determine which within-field variables would be informative of soybean arthropod counts in an attempt to move toward site-specific pest management in this crop. Two soybean fields were grid-sampled for pestiferous and predaceous arthropods, plant productivity estimates, and abiotic variable characterization in 2017-2018. Negative binomial, zero-inflated models were used to estimate presence and counts of soybean arthropod taxa based on normalized difference vegetation index (NDVI), soybean plant height, soil electrical conductivity (ECa), elevation, and calendar week. Among all variables, calendar week was the most reliable predictor of arthropod counts, as it was a significant predictor for a majority of all taxa. Additionally, counts for a majority of pestiferous taxa were significantly associated with distance from the field edge, elevation, soybean plant height, and NDVI. Although site-specific pest management has the potential for reduced management inputs and increased profitability over conventional management (i.e., whole-field) practices, management zones must first be clearly defined based on the within-field variability for the variables of interest. If site-specific pest management practices are to be applied in soybean, calendar week (and associated soybean phenology), soybean plant height (and associated elevation), and NDVI may be useful for describing the distributions of pests, such as kudzu bug, Megacopta cribraria (Hemiptera: Plataspidae) (Fabricius), green cloverworm, Hypena scabra (Lepidoptera: Erebidae) (Fabricius), velvetbean caterpillar, Anticarsia gemmatalis (Lepidoptera: Erebidae) (Hübner), and soybean looper, Chrysodeixis includens (Lepidoptera: Noctuidae) (Walker).

Core‐marginal dynamics interact with sex and temperature to influence morphology of the rapidly expanding invasive kudzu bug (Megacopta cribraria)

1. Invasive species present unique natural experiments through which to study evolutionary processes associated with spatial spread, especially with respect to rapid morphological evolution of dispersal‐related traits.2. The kudzu bug,Megacopta cribraria(Fabricius), established and expanded radially across the southeastern United States beginning in 2009. Here,M. cribrariais used as a model for studying rapid morphological change across an introduced range.3. Kudzu bugs were collected throughout the invasive range to test the hypothesis that body and wing size would increase with greater distance from the invasion source owing to dispersal‐related selection. The effects of sex, local temperature, and latitude were also evaluated to investigate how such factors might influence body and wing size across this range expansion.4. Females were significantly larger than males across all traits. Distance from the invasion core, temperature, and their interaction significantly influenced body size and wing length, suggesting thatM. cribrariawere larger near the invasion periphery. This was especially pronounced at cooler sites. Sex and distance were found to both weakly influence wing length after removing the effect of body size, suggesting that wing size may be disproportionally larger along the invasion front.5. The combined results indicate that a combination of spatial, environmental, and sexual factors must be considered when examining or predicting invasive species responses to new selective pressures and environments.

Efficacy of Selected Insecticides on Kudzu Bug in Soybean, 2015

Efficacy of Selected Insecticides on Kudzu Bug in Soybean, 2015

Role of abiotic factors on the incidence of major insect pests of pigeonpea, Cajanus cajan (L.) Millsp

During present studies incidence of pod fly; Melanagromyza obtusa Malloch (Diptera: Agromyzidae), thrips; Megalurothrips usitatus Bagnall (Thysanoptera: Thripidae), pod bug; Clavigralla gibbosa (Spinola) (Hemiptera: Coreidae), Kudzu bug; Megacopta cribraria Fabricius (Hemiptera: Plataspidae) and cow bug; Otinotus oneratus (Walker) (Hemiptera: Membracidae) was found negatively correlated with temperature (both maximum and minimum) and rainfall whereas positively correlated with morning relative humidity except thrips. It was also found that Leaf webber; Grapholita critica (Meyr.) (Lepidoptera: Tortricidae) incidence was positively correlated with temperature and morning relative humidity. Regression analysis showed that minimum temperature significantly contributed more than 49% variation to the high population buildup of the C. gibbosa, M. cribraria and O. oneratus insects. The present study also report first time the effects of different abiotic factors on the incidence of Kudzu bug; M. cribraria.

First Record of Ooencyrtus nezarae (Hymenoptera: Encyrtidae), a Parasitoid of Megacopta cribraria (Hemiptera: Plataspidae) in Florida

During our study on egg parasitoids of the kudzu bug in Florida, we collected egg masses of Megacopta cribraria on kudzu and catnip for laboratory rearing. We found a new egg parasitoid, Ooencyrtus nezarae on M. cribraria, which represents the first Florida record of this parasitoid species. The wasp is widely distributed in Asia, and it will strengthen biological control of the kudzu bug in Florida. Future studies will assess comparative parasitism levels of Paratelenomus saccharalis and O. nezarae, as well as the dynamics of populations of these biological control agents.

Mechanisms of Soybean Host-Plant Resistance Against Megacopta cribraria (Hemiptera: Plataspidae)

A number of soybean varieties traditionally bred for resistance to various soybean arthropod pests have been identified as resistant to Megacopta cribraria (F.) (Hemiptera: Plataspidae). However, the mechanisms of host-plant resistance (HPR) in this system are not understood. The goal of this study was to identify the mechanisms of resistance by examining the role of plant volatile organic compounds (VOCs) and free amino acids (FAAs) among 16 soybean varieties. Choice and no-choice cage experiments identified several soybean varieties that demonstrated antixenosis as well as antibiosis. However, resistance varied over time in certain soybean varieties, such as N02-7002 and PI567352B. Mean nymph number from choice experiments had positive correlations with the FAAs asparagine, tryptophan, alanine, phenylanaline, and serine; negative correlation with leucine and threonine. Four plant volatiles, hexanal, 2-pentylfuran, beta-cyclocitral, and cis-9-hexadecenal, were positively correlated with subsequent nymph development, whereas n-hexadecenoic acid was negatively correlated with nymph number only, in adult choice cage experiments. This study contributes to understanding the mechanisms of HPR through associations with plant VOCs and FAAs in relation to M. cribraria development and provides useful knowledge for developing soybean varieties for M. cribraria management.

Water Loss and Desiccation Tolerance of the Two Yearly Generations of Adult and Nymphal Kudzu Bugs, Megacopta cribraria (Hemiptera: Plataspidae).

Water loss rate, percentage total body water content (%TBW), cuticular permeability (CP), and desiccation tolerance were investigated in adult and immature stages of the invasive kudzu bug, Megacopta cribraria (Fab.) (Hemiptera: Plataspidae), a serious soybean pest and an urban nuisance. Adults and all five nymphal instars were weighed prior to and 2, 4, 6, 8, 10, and 24 h after desiccated at 30 ± 1°C and 0-2% RH. Both % initial mass and %TBW loss increased linearly with time of desiccation. Rates of loss ranged from approximately 1-7%/h. Mortality occurred at 10 h after desiccation. Desiccation tolerance (%TBW lost at death) ranged between 25.6% for first-generation adult females and 75% for first-generation fifth-instar nymphs. First-generation first-instar nymphs had significantly greater %TBW (88.9%) than the other generations and instars, whereas second-generation fifth instars had the lowest %TBW (62.4%). The CP value of first-generation adult females (12.3 ± 1.6 µg cm-1 h-1 mmHg-1) was the greatest across generations. First-generation first instars had the greatest mass loss (111.11 mg/g) among all instars and generations, whereas overwintered second-generation adult females had the lowest mass loss (18.39) across generations. This study demonstrated that desiccation stress differentially affected the survival of adult and nymphal kudzu bugs and may imply that environmental stress can affect the relative abundance of this species in the fields and around homes.