Abstract Background Saudi Arabia faces a growing antimicrobial resistance (AMR) problem, exacerbated by the widespread use of antibiotics in clinical and agricultural settings. Despite this, AMR surveillance has been limited, particularly in regions like Najran, making this study critical for informing local and national public health strategies. Aim This study explored the prevalence of multidrug-resistant, extensively drug-resistant, and Pandrug-resistant bacteria in Najran, Saudi Arabia. Study design This is a cross-sectional study. Methods This study included 559 diverse clinical samples (urine, wound, etc.) collected from various departments in King Khaled Hospital, Saudi Arabia. The Phoenix BD instrument was used for complete bacterial identification and antibiotic sensitivity testing. Demographic and clinical data were analyzed statistically. Results Of the 559 samples, the culture positivity rates were as follows: 51% for Escherichia coli, 22% for Staphylococcus aureus, 14% for Klebsiella pneumoniae, 6% for Acinetobacter baumannii, 6% for Pseudomonas aeruginosa, and 1% for Enterococcus faecium. Majority of the cases were from male (57%), and age 50+ (59%) recorded highest cases. Participants from outpatient department (OPD) ward recorded the highest (56%) cases, while urine sample recorded the highest (49%) cases. About 84% of the isolates were multidrug‑resistance (MDR), 10% were extensively drug‑resistance (XDR), and 6% pandrug‑resistance (PDR). Our analysis showed high sensitivity to Oxazolidonone, Tetracycline, carbapenems, Lipopeptide, Aminoglycopeptide classes of antibiotics. Conclusions The study revealed a high prevalence of drug resistance, highlighting the critical importance of continued surveillance and research to mitigate the spread of antimicrobial resistance and preserve the effectiveness of existing therapies.
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