Kinetics of order-disorder transition at antiphase domain boundary (APDB) formed between DO22 (Ni3V) phases during stress aging was investigated using microscopic phase field model. The results demonstrated that whether order-disorder transition happens or not depends on the atomic structure of the APDB. Accompanied with the depletion of V and enrichment of Ni and Al, order-disorder transition happened at the APDB (001)//(002). Whereas at the APDB {100}·1⁄2[100], which remains ordered with temporal evolution, Ni and Al enrich and V depletes. Composition evolution of APDB with order-disorder transition favors the nucleation of the L12 and disordered phase. Some of the grains grew bigger while the others disappeared, accompanying the formation of disordered phase layer during order-disorder transition of APDBs, and the order-disorder transition of APDBs can be considered as accompanying process of coarsening of ordered domain phases and growth of disordered phases.