The operational safety assessment of the unmanned vehicles is crucial for their subsequent practical use. The safety limit criteria used for the operation assessment, especially values closely related to the safety of persons and their vulnerability to collision with these vehicles, represent nowadays a significant limitation. This contribution presents the currently discussed and proposed human safety criteria for the UAS, evaluates the available data from the collision dynamic tests and computer modelling and provides the possibility to compare and evaluate these criteria on a validated set of data. A total of five small UAS, three multi-rotor quadcopters and two fixed-wing aircrafts were used to verify the current methods and serve as a basis for the assessment. The results of the measurements obtained through the crash tests demonstrate the excessive restrictiveness of the currently used kinetic energy threshold values and the limited value of some proposed criterions, e.g. the blunt criterion. On the contrary, they point to the appropriateness of the application of car vulnerability criterions. The UAS mass or kinetic energy tests represent an easily definable threshold value, however, the available data sets and the tests performed showed limitations of its applicability for the deformable and fragile UAS structures. The article aims to verify, based on its own impact tests, the adequacy of the evaluation of the safety criteria of the UAS operation in relation to the development of the current legislation that defines the conditions of use of these machines.