Kind Of Concentration Research Articles

Optimization of Snail (Achatina fulica) Mucus Gel Formula with CMC-Na as a Gelling Agent Healing Periodontitis

The content of snail (Achatina fulica) mucus chemical compounds that have been tested by researchers quantitatively are: Heparan Sulfate 16.45 mg/100 g, Acharan Sulfate 21.33 mg/100 mg, Acahtin isolate 36.06 mg/100 mg, Ca2+ Ion 86.12 mg/100 g, Beta Agglutinin 58.22 mg/100 g, Achasin Protein 102.22 mg/100 g, Glycokonyugat 8.86 mg/100 g, qualitatively snail mucus gel contains chemical substances contained in snail mucus gel are: Allantoin, Collagen, Elastin, Glicolic, Vit A and and C, Antibiotic peptide, Protein, Enzyme, Hyaluronic Acid, Copper Peptide, Antimicrobial peptide, Iron, Zinc, Proteoglycans. The purpose of this study was to obtain the optimal concentration of Na CMC as a gelling agent in the manufacture of snail mucus gel, obtain the optimal formula from the optimal formula variation, make a snail mucus gel preparation that serves as a cure drug for periodontitis, test snail mucus gel with optimum concentration in rats for the treatment of periodontitis. Research methods: true experimental laboratorium, determination, preparation of snail mucus chemical compounds, optimization and determination of Na CMC, optimization and determination of glycerin, optimization and determination of sodium lauryl sulfate and snail mucus preparation formula. The results of the study obtained optimization of gel preparations with three kinds of concentrations, namely 5%, 7.5%, and 10%, obtained the results of the stability test of the preparation declared stable, tasteless organoleptical viscous liquid, pH 6.75; 6.81; 6.88; 6.79; 6.79; 6.84 evenly distributed 5.6; 6.1; 6.5; 6.79; 6.79; 6.84; adhesion 12.45g/cm2; 15.80: g/cm2 ; 17.20; 12.50; 12.50;17.50; 17.20; 20.40; 15.40; 15.40; 20.05 viscosity 17.50; 17.20; 20.40; 15.40; 15.40; 20.05 cp and turbulent flow type (4050 Reynold), 4100; 4095; 4050; 4050; 4080. The conclusion of this research is the optimal concentration of Na CMC as a gelling agent in the manufacture of snail mucus gel is 10%.

In Vitro Evaluation of Seagrass Extracts as a Prevention of Microfouling Formation

Microfouling is the attachment and colonization of bacteria and microalgae on the surface of objects immersed in the sea. So far, the control of biofouling on the surface of marine hulls has been using paints containing Tributylin Organotin (TBT) which have adverse effects on non-target organisms and are not friendly to the environment. The development of environmentally friendly antifouling paint is a solution to overcome the growth of biofouling. This study aimed to evaluate in vitro extract of Thalassia hemprichii as an anti-microfouling agent. Extraction of bioactive from Thalassia hemprichii was carried out by maceration. The filtrate is divided into five kinds of concentration, namely 0%, 25%. 50%, 75%, and 100% were then added to paint A as an anti-microfouling agent. The observed variables were microfouling biomass and the density of bacteria and microalgae attached to the steel plate. The results showed that with increasing concentration applied to the plate caused a decrease in the number of bacteria. The average decrease in each bacterial concentration was 84 x 10^6 CFU/mL, 65 x 10^6 CFU/mL, 41.5 x 10^6 CFU/mL, 28.7 x 10^6 CFU/mL, and 15.8 x 10^6 CFU/mL, respectively. Microalgae attached to the steel plate also experienced a reduction with the average values sequentially being 37 x 10^4 cells/mL, 14 x 10^4 cells/mL, 8 x 10^4 cells/mL, 5 x 10^4 cells/mL, and 2 x 10^4 cells/mL. Likewise, the microfouling biomass on steel plate also decreased in amount with the average values being 99.27 mg, 93.4 mg, 66.43 mg, 59.13 mg and 30.2 mg, respectively. Thus, Thalassia hemprichii extract has the potential to prevent the formation of microfouling.

Pengaruh Aplikasi Actinomycetes terhadap Serangan Fusarium oxysporum Schlecht. f.sp. cepae (Hanz.) Synd. et Hans. Penyebab Penyakit Layu pada Bawang Merah (Allium ascalonicum L. var. Mentes)

Fusarium oxysporum Schlecht. f.sp. cepae (Hanz.) Synd. et Hans. is a fungus that causes Fusarium wilt in shallots, which causes damage to tubers and reduces yields by more than 50%. This disease is difficult to control, because it can form spores that can survive in the soil for a long time, and are saprophytic on the remains of other plants. This study was conducted to determine the effect of the application of 5 kinds of Actinomycetes isolates (SW13, SW5, BYM1, BYM4, and SIO 5), with 3 kinds of concentrations (5 ml, 10 ml, and 15 ml/plant) on the Mentes variety shallots. Fusarium oxysporum. The results showed that the application of the isolate code SIO5 to the Mentes variety of shallots gave the best effect in suppressing the attack of Fusarium oxysporum by 52.2%. In addition, the suspension of SIO5 isolate also had a positive effect on plant height of 41.5 cm, number of tillers of 8.89, and tuber weight of 42.84 grams. While the type of concentration did not have a good effect on the incidence of disease, plant height, and the number of tillers and tuber weight.

STUDY OF OLIGO CHITOSAN IN EFFORTS TO IMPROVE THE GROWTH QUALITY OF SHALLOT (Allium ascalonicum L.)

This research is intended to answer the obstacles in the cultivation techniques of shallots, especially in overcoming the low quality of growth, which in turn affects the yield and production of onions. In this experiment, spraying three types of frequency and four kinds of oligo-chitosan concentrations was tried throughout the growing onion period. Oligo-chitosan as a supplement, is given to the onion plants periodically, in various concentrations, in three different types of oligo-chitosan spraying, which they are (F1) given to plants aged 15 DAP and 45 DAP; (F2) given to plants aged 15 DAP, 30 DAP, and 45 DAP; and (F3) given to plants aged 15 DAP, 25 DAP, 35 DAP, 45 DAP. The oligo-chitosan concentration consists of four kinds of concentration, consisting of 0 ‰ (K0) as a control, 1 ‰ (K1), 2 ‰ (K2) and 3 ‰ (K3). The experiment was carried out in the field using a polybag, with a completely randomized environmental design with a 95% accuracy level. To determine the effect of treatment on the quality of growth of shallots, diversity analysis was carried out. From preliminary observations, it shows that the onion bulb growing power as a seed is quite good, while the treatment using oligochitosan gives significant results on increasing plant vigor, compared to not using oligochitosan. As for further observations that at plant height, the number of leaves and the number of tillers up to 50 days after planting, although it does not show any real effect of the treatment that was tried, but it can be suspected that the more frequency treatment given oligo-chitosan, more gives a better chance of yield than a few frequencies, while an oligo-chitosan concentration of 1 ‰ is sufficient to improve the quality of plant growth, then it can be expected that F2K1 will give higher tuber yields.Keyword: shallot, oligo-chitosan spraying frequency, oligo-chitosan concentration

Produção e formação audiovisual “fora do eixo” no Brasil: o caso do Instituto Federal de Goiás

A produção cinematográfica e audiovisual, a exemplo dos grandes grupos de média, esteve historicamente concentrada nas mãos de poucos agentes em relação ao todo populacional. Este tipo de concentração favorece discursos hegemônicos e representações alienadas de culturas que não produzem seu próprio conteúdo. As políticas públicas de descentralização da produção audiovisual brasileira no século XXI estão a desempenhar um papel importante e bem-sucedido de aumento da produção em regiões fora do eixo Rio-São Paulo, principal centro econômico e de produção dos média do Brasil. As instituições de formação audiovisual que estão “fora do eixo” devem estar atentas a esta organização, preparando os estudantes para os desafios técnicos, sociais e culturais da descentralização da produção e do discurso. O Instituto Federal de Goiás (IFG) oferece desde 2015 o Bacharelado em Cinema e Audiovisual em Goiás, uma pequena cidade da região centro-oeste do Brasil. O curso tem obtido resultados importantes advindos de sua proposta pedagógica ao mesmo tempo em que tenta se consolidar e estabelecer ações de cooperação com outras instituições fora dos principais centros de média de seu país e do mundo.

KANDUNGAN SENYAWA FLAVONOID DAN ANTOSIANIN EKSTRAK KAYU SECANG (Caesalpinia sappan L.) SERTA AKTIVITAS ANTIBAKTERI TERHADAP Vibrio cholerae

The aim of this research to determine the concentration of flavonoid and anthocyanin compounds in sappan wood extract (Caesalpinia sappan L.), as well as to determine the concentration of inhibitory power against the growth of Vibrio cholerae. This experimental design study uses 5 kind of concentration, which are 20%, 40%, 60%, 80%, 100%. The analysis were repeated 3 times and resulting in 15 experimental units. The data were presented in descriptive statistic with tables, drawings and discussed. The results showed that the extract of sappan wood contained flavonoid compounds of 6,02%, anthocyanin compounds of 2,43% and was able to inhibit the growth of Vibrio cholerae with optimal concentration at a concentration of 80% with an average 20,1 mm and very strong inhibition categories.

Distraction and digital media

Research shows that multitasking implies that we use more time, remember less, learn less and perform worse than if we single-task.The article: ”Mobile Phones in the Classroom” (Kuznekoff et al. 2015) is worth highlighting as it is one of the most cited articles on multitasking. It shows how damaging written interaction is for learning in teaching situations. But does this mean that social media and written interaction should not be used in teaching? And that social media and written interaction only can harm learning? In this paper we provide a critical review of Kuznekoff et al. (2015). We argue that their laboratory experiment is taken out of real educational context and that this distorts the results they achieve. With a background in medium theory and media didactics we analyze the laboratory experiment and point out its shortcomings. Based on a qualitative real-life action research project we demonstrate that it is possible to set up conditions that make written interaction (through social media like Twitter) beneficial to education. Furthermore, we clear up the issue theoretically by distinguish between and discuss teaching situations in the new medium environment in relation to different kinds of concentration: diffused concentration (multitasking), non diffused concentration (singletasking) on one axis and the use of only one medium (singleplexing) and the use of several media (multiplexing) on another ortigonal axis.

UJI FITOKIMIA EKSTRAK BUNGA LAWANG (Illicium verum Hook.f) DAN DAYA HAMBATNYA TERHADAP Staphylococcus aureus

This study aims to determine the levels of tannin and flavonoid compounds, as well as to determine the concentration of the extract of star anise (Illicium verum Hook.f) was most appropriate by used aquades solvent against the growth of Staphylococcus aureus. This research used experimental method with 5 kind of concentration which are 20%, 40%, 60%, 80% and 100%, each treatment was repeated 3 times to obtain 15 experimental units. The observed variables were quantitative testing of flavonoid and tannin compounds and create an inhibition zone produced by antimicrobial activity of the extract of star anise on Staphylococcus aureus. The data of this research are presented in the form of tables, figures and analyzed descriptively. The results showed that the extract contained flavonoid compounds of 0.106% and tannins of 1.018%. The extract of star anise can inhibit Staphylococcus aureus at a concentration of 40% with an average 7.03 mm inhibition zone.

Pengaruh Kombinasi Ekstrak Pahit Pare (Momordica charantia) dan Sapodilla (Manilkara zapota) terhadap Pertumbuhan Bakteri Salmonella typhi dengan Menggunakan InVivo pada Tikus Kecil Usus

Salmonella typhi bacteria is a main cause of typhoid fever. The fever can be healed by using medical and traditional way. Bitter melon plantand Sapodilla have a lot of active compound, having potential as antimicroba as like salmonella typhi. The problem of this research is to find out whether the extract combination of bitter melon and sapodillais effective in inhibitingthe growing of Salmonella typhi in mice small intestine. Thisresearch aims to determine inhibitory concentration in the growing of Salmonella typhi in mice small intestine. 
 This researchused experimental design with post test group. This research used experimental animals that is mice which is given Salmonella typhi and is given the extract of bitter melon and sapodilla in different kinds of concentration with sonde. The measurement of bacteria is first by taking small intestine mucosa and put it SSA media. Then, it is being identified and accounted. After that, the data result is being tested by ANOVA with the data requirement that is normally distributed and homogeneous by using SPSS with α=0.05
 The research result shows that there is 300 groups in positive control, while on the other way there is no groups found, except in 100% bitter melon group of seven bacteria groups. After being tested, the data shows that the score is not normal (sig. 0.000 < α =0.005). As the result, the data is being tested by using non parametric test and Kruskal-wallis test. Lastly, the test result shows there is a significant difference among treatment groups (sig. 0.013 < α =0.05)
 This research concludes that the combination extract of bitter melon and sapodilla effectively can inhibit the growing of Salmonella typhi by using in vivo in the mice small intestine.

Effect of co-existing ions on lead leaching behaviour from hardened cement paste

Recently, in the concrete field, the use of industrial wastes for concrete materials is expected for environmental reasons. However, these wastes may contain hazardous heavy metals. It is well known that cement paste has an ability to fix heavy metal ions, whereas it was also found that absorbed heavy metal ions can easily leach from cement paste in the presence of a CaCl2 solution. The present study aims to investigate the lead leaching behaviours from hardened cement pastes in various chloride solutions by tank leaching tests. The water-to-cement ratios of the cement paste specimens were 0.40 and 0.55. Lead was added to the cement pastes at 1 mass% of cement. Each chloride solution for the tank leaching test had three kinds of concentrations (5%, 10%, and 20%). After the tank leaching tests, the chemical changes of the specimens were analysed to discuss the effect of co-existing ions in the solutions. The results indicate that the cumulative lead leaching amount is related to the types of chloride solution as well as the concentrations of the solutions. The lead leaching amount in the CaCl2 solution was much higher than that in other solutions.

The collective influence of 1, 25-dihydroxyvitamin D3 with physiological fluid shear stress on osteoblasts

1, 25-dihydroxyvitamin D3 (1, 25 (OH)2 D3) and mechanical stimuli in physiological environment contributes greatly to osteoporosis pathogenesis. Wide investigations have been conducted on how 1, 25-dihydroxyvitamin D3 and mechanical stimuli separately impact osteoblasts. This study reports the collective influences of 1, 25-dihydroxyvitamin D3 and flow shear stress (FSS) on biological functions of osteoblasts. 1, 25 (OH)2 D3 were prepared in various kinds of concentrations (0, 1, 10, 100 nmmol/L), while physiological fluid shear stress (12 dynes/cm2) was produced by using a parallel-plate fluid flow system. 1, 25 (OH)2 D3 affects the responses of ROBs to FSS, including the inhibition of NO release and cell proliferation as well as the promotion of PGE2 release and cell differentiation. These findings provide a possible mechanism by which 1, 25(OH)2 D3 influences osteoblasts’ responses to FSS, thus most probably providing guidance for the selection of 1, 25(OH)2 D3 concentration and mechanical loading in order to produce functional bone tissues in vitro.

Study on Surface Tension of Membrane Made in Cellulose Diacetate

The value of surface tension is important for choosing oil in CA fibers production. So indirect method were used to analyze the surface tension of CA fibers in this paper. First, Chips of CA were dissolved in acetone solution with 5%, 10%, 15% three kinds of concentration. The three kinds of concentration of the solution were heated 5 hours with 55°C and then standing and defoaming for 24h. Squeeze the solution with two flat glass slides to make the membranes. Second, choose deionized water, Glycerin, Formamide, Bromonaphthalene and Diiodomethane with different polarity as tested liquids to measure the contact angle of CA membrane with JC2000C contact angle meter. Then, Zisman’s linear extrapolation method was used to measure the critical surface tension of CA membranes. On the other hand, S. Wu’S harmonic equation on average method was used too for comparison. The value of two different methods was the same, 42 mN/m, and the result indicated that the estimated method was reliable.

The Expression of IL-8 in Gingival Rattus novergicus After Exposed by Silver Gel Nano Particles 15 µg/ml

<p><strong><em>Background:</em></strong><em> The</em><em> </em><em>uses of silver </em><em>nanoparticle </em><em>are growing so fast, especially in the medical science</em><em>, it’s </em><em>used in many kinds of concentration</em><em>. In dentistry, it’s used </em><em>to decrease halitosis and periodontal diseases, and </em><em>also </em><em>wound healing</em><em> process</em><em>. </em><em>I</em><em>t can affect the viability of the cell</em><em>s, give the bad effects to the human’s health and environment if </em><em>we </em><em>use it in a long-term and in an uncertain concentration. <strong>Purpose</strong></em><strong><em>:</em></strong><em> The aim of the study was to analysis the</em><em> </em><em>healing process of gingival fibroblast in Rattus novergicus after exposed </em><em>by </em><em>15</em><em> </em><em>µg/ml</em><em> </em><em>silver</em><em> gel </em><em>nanoparticles.</em> <strong><em>Materials and Methods:</em></strong><em> This study </em><em>uses </em><em>9 male wistar rats were divided into 3 groups. Group A: Cut (hurt it) in the oral gingiva and exposed to Ag-Np Gel 15 µg/ml for 3 days. After 3 days, they were sacrificed and cut the gingival fibroblast off 3x4 cm size with scalpel. Group B: cut in the oral gingiva and exposed to Ag-Np Gel 15 µg/ml for 5 days. After 5 days they were sacrificed and cut the gingival fibroblast off 3x4 cm size with scalpel. Group C: cut in the oral gingiva and exposed to none for 3 days then cut the gingival fibroblast off 3x4 cm size with scalpel. The expressions of</em><em> IL-8 </em><em>in the </em><em>wound healing </em><em>process were </em><em>painted</em><em> by Im</em><em>m</em><em>unohistochemical </em><em>method</em><em>. This data was analyzed by using the t-test method. <strong>Results:</strong></em><strong><em> </em></strong><em>General characteristic of IL-8</em><em> is </em><em>show</em><em>n in</em><em> brown color im</em><em>m</em><em>unoreactive expression on macrophag</em><em>e</em><em> via immunohistochemic</em><em>a</em><em>l painting with monoclonal antibody anti IL-8</em><em>. </em><em>Mean expression numbers of</em><em> IL-8 </em><em>in the group A = </em><em>11,7</em><em>; group B = 1</em><em>6,3</em><em>; and group C (control) = </em><em>28,3</em><em>. T-test sign.number of group A & C = 0,00</em><em>4</em><em>; group B & C = 0,000. <strong>Conclusion</strong></em><strong><em>:</em></strong><em> The exposure of 15 µg/ml silver gel nanoparticle to gingival fibroblast of Rattus novergicus reduce</em><em>s</em><em> the expressions of</em><em> IL-8 </em><em>in the</em><em> day-3 and day-5 post exposure</em><em>. So, it may accelerate the wound healing process.</em></p><p><strong><em>Keywords:</em></strong><strong><em> </em></strong><em>H</em><em>ealing</em><em>, silver, nanoparticles, </em><em>IL-8</em></p><p><strong><em>Correspondence:</em></strong><em> Kharinna Widowati, </em><em>Department of Science Mouth Disease,</em><em> </em><em>Faculty of Dentistry,</em><em> Hang Tuah</em><em> University,</em><em> </em><em>Arif Rahman Hakim 150</em><em>,</em><em> Surabaya</em><em>,</em><em> </em><em>Email: <span style="text-decoration: underline;"><a href="mailto:kharinna.widowati@gmail.com">kharinna.widowati@gmail.com</a></span></em><em></em></p>

REFLEKSI PSIKOLOGI MUSIK DALAM PERILAKU MASYARAKAT SEHARI-HARI

Music is a branch of art close to and present in human’s life every day. Through the social study perspective, this kind of concentration is usually called musical semiology or how music functions as an art creation in the society. In the daily life, men always listen to music intentionally or unintentionally. Therefore, music is inseparable from human life. The logical consequence is an effort to improve the role of music in the society, along with the fast development of media technology and culture. Art as a human creation has various functions which are not only for artistic interests. From the psychological context, music functions in and influence the increase of intelligence, therapeutic benefit that are not detached with its cultural, technical, and methodical background. Here, as part of the hybrid between music and psychology, musical therapy adopts relevant psychoterapic techniques. The term musical therapy has also been used in the curriculum of educational institution and foundation for children with specific needs of education with qualification of education or psychology for the specific needs.

The Design of Concentrator in Power Energy Information Acquisition System Based on the Internet and Zigbee

Employing S3C2410 as the core component, a kind of concentrator in power energy information acquisition system based on the Internet and the short-distance communication technology Zigbee has been designed in this paper. The structure and function of the system has been introduced too. The Internet and Zigbee communications are realized and parts of the circuits and flow charts are given. This system uses the short-distance communication technology Zigbee to realize the data transmission between concentrator and collector, and then uses the Internet to carry the data to the superintendent center, and realizes the remote brail and management function. Empirical results show that this system can primly realize the itinerant excerption of the ammeters, the analysis of the statistical electric dosage, and the remote control of switch on and off. This system has the low cost, high reliability and stability, it can advance the automation level of the electric power management, and has a good foreground.

Chemical Control of Flavone from Forestry Waste<i> C.cathayensis </i>Exocarp on Nutritive Growth of Crops

Using potted wheat, corn, soybean and mung bean as the materials, the effects of different treatments with six kinds of concentration ( 0.1 ~ 2.0 / mg.L-1 ) of flavone extract from C.cathayensis exocarp on the relative electrical conductivity, root activity, nitrate reductase activity and nutritive absorption of four kinds of newborn crop seedlings roots were studied. The results showed that within the range of concentration of 0.1~ 0.5/mg.L-1, the flavone extract promoted the nutritive growth of the four kinds of crops at seedling stage. The root activity of the wheat and mung bean seedlings were 0.29 and 0.35/ug.dg-1.h-1, 40-24% higher than CK, the nitrate reductase activity of the corn and soybean seedlings were 49.9 and 53.1/mg.L-1, with an increase of 54-24%. The effects of flavone extract on the four kinds of crop reached the significant or extremely significant level (P < 0.01, P < 0.05). A suitable concentration of flavone extract could significantly promote the root absorption on N, K, Ca, Mg, Fe, Mn, Cu and Zn, but in view of four kinds of crop, the different concentration has different promotive or inhibitive effect. Research results showed that the appropriate concentration of flavone extract from C.cathayensis exocarp could promote the growth of four kinds of crop seedlings, and improve the effect of the mineral nutrient absorption of seedling newborn roots.

Concentration of trace elements in small lakes of the Ingoda basin (Chita Region, Russia)

By the example of the Ingoda basin (Transbaikalia) including a system of small lakes, the role of two leading processes of concentration of trace elements in small reservoirs (water–rock interaction and evaporation) has been estimated. It is shown that the accumulation of U, As, Se, Sb, and Ge does not depend on the kind of concentration. Their contents increase linearly with total dissolved solids in both lake and feeding waters. Accumulation of Sc, V, Y, Th, REE, and W is mainly due to evaporation, with their maximum concentration by three to four orders of magnitude. Only U, W, and REE, whose contents in the lakes reach n⋅100 μg/L, are of practical interest.

Effect of Flavone from Forestry Waste <i>C.Cathayensis</i> Exocarp on Photosynthetic Characteristics of Crops

Using six kinds of concentration (ranged from 0.0 to 2.0mg.L-1) of flavone extract from C.cathayensis exocarp treats corn, soybean, wheat and mung bean seedlings, in order to study the photosynthetic rate, stomatal conductance, transpiration rate, intercellular CO2 on the seedling leaf. Results show that effects of flavone extract (0.1-0.5mg.L-1) increase content of the chlorophyll a, b and total chlorophyll, promote photosynthetic rate, stomatal conductance, transpiration rate, proper increase cell gap inside the CO2 concentration.0.1mg.L-1 treatment effect is the most obvious compare with the control, the total chlorophyll and photosynthetic rate increase by 20.9 % and 21% respectively; More than 0.5mg.L-1, the chlorophyll content, photosynthetic rate, stomatal conductance, transpiration rate decrease, the intercellular CO2 rapid increase. Research shows that, the appropriate concentration of flavone can increase the chlorophyll content in the leaf, promote stomatal opening, increase of intracellular CO2 supply, improve leaf photosynthesis efficiency.

Influence of proliferation and ostogenic differentiation of 1,25 (OH)2VD3 on hUCMSCs in vitro

Objective To establish the separation and expansion method of human umbilical cord derived mesenchymal stem cells (hUCMSCs) and explore the influence of 1,25 (OH)2VD3 on hUCMSCs in vitro.Methods UCMSCs were cultured from adherent tissue pieces and detected by immunohistochemistry.Taking the third generation with good growth state UCMSCs make intervene experiment.Set 10-7 mol/L,10-9mol/L and 10-11 mol/L 3 kind of concentration of 1,25(OH)2VD3 as the experimental group,and a control group (DMEM medium).The morphologic change of UCMSCs was observed by inverted microscope.Observe the cell proliferation by the method of MTT.Detect alkaline phosphatase assay and type Ⅰ collagen assay from immunohistochemistry.And the calcium tubercle formation would be examined after 21 days.Results UCMSCs were cultured from adherent tissue pieces and strongly positive for CD44,CD105 and negative for CD34,CD45.consistent with the hUCMSCs characteristics.Under different concentration of 1,25 (OH)2VD3hUCMSCs proliferation were promoted within 7 days but were suppressed beyond 7 days.The high doses group ( 10-7 mol/L group ) obvious inhibitted the stem cell proliferation.Different concentrations of 1,25 (OH)2VD3 and days have interactive effect (P ＜ 0.05).The differences between different groups with ALP and type Ⅰ collagen assay has statistical difference (P ＜ 0.05).The secretion of ALP and type Ⅰ collagen assay of 10-7 mol/Lconcentration group was higher than others.Typical mineralization nedus was found in intervene groups.Conclusion HUCMSCs can be successfully cultured from the adherent tissue pieces.1,25(OH)2VD3 can effectively induce hUCMSCs to differentiate into osteoblasts in vitro. Key words: Umbilical cord derived mesenchymal stem cells (UCMSCs); 1,25(OH)2VD3; Differentiation; Osteoblasts

Development of a Novel Electrochemical Surface Plasmon Resonance Instrument Based on a Mini Three Electrode Flow Cell

The construction and performance of a novel electrochemical (EC) surface plasmon resonance (SPR) instrument based on a mini three electrode flow cell that was designed and manufactured by MEMS technology is described. The working electrode is 50nm gold and counter and reference electrodes, made of Au and Ag/AgCl separately, are 0.6 mm in diameter. The flow channel is made of polydimethylsiloxane (PDMS) with 1mm in width, 200μm in depth, 5μL in volume. The process for activating and modifying the working electrode is given by the real-time SPR detection. The Cyclic voltammetry (CV) and SPR detection are conducted simultaneously in potassium ferricyanide solution system. It is proved that the signal of redox reaction and the change of refractive index have a well correlation by the potential sweep experiment. Four kinds of concentration of potassium ferricyanide are analyzed and the peak oxidizing current, obtained from the curve, is proportional to the respective concentration of the sample. The EC-SPR experiment is also carried out in CuSO4 system and the results is similar to potassium ferricyanide.