Carbon materials have been extensively used in scientific research and industrial production. Thereby, a kind of nanospheres of carbon (NSC) was prepared from sucrose, focusing on its physical and chemical structures, getting results that: (1) the yield of NSC is in average of 30.68% of the mass of sucrose, corresponding to 61.97% of carbon conversion. (2) The diameter of NSC is in the range of 150–200 nm, containing 1351.41 m2/g of surface area, which is contributed by the pores in average of diagram of 3.32 nm. (3) The apparent molecule formula (AMF) of NSC can be expressed as C11H2O with unsaturated degree of 11, which is the sum of the numbers of double bonds and rings. (4) It contains the groups of C = C, C-C, C-O-C and C-H; especially, the ratio between the double carbon bonds and single carbon bonds is 1.0. Therefore, this research successfully prepares a kind of special carbon materials by bio-resource, and does benefit to the theory for chemical structure analysis of carbon materials.