kHz Ultrasound Research Articles

Accelerated Mechanophore Activation and Drug Release in Network Core‐Structured Star Polymers Using High‐Intensity Focused Ultrasound

The ultrasound (US)‐induced activation of mechanophores embedded in linear polymers (LPs) is the most widely employed technique to realize chemical function by polymer mechanochemistry. However, the commonly used US frequency in this context is around 20 kHz, producing strong inertial cavitation limiting biomedical applicability. Herein, 20 kHz US and 1.5 MHz high‐intensity focused US (HIFU) are investigated to drive disulfide mechanophore activation and mechanochemical polymer chain scission in network core‐structured star polymers (NCSPs). It is found that the efficiency of activating disulfide mechanophores in NCSPs using 1.5 MHz HIFU irradiation is similar to the efficiency achieved with 20 kHz sonication. This is quantified by ‘turn on’ sensor molecules leveraging the Michael addition of the mechanochemically generated thiol groups and subsequent retro Diels–Alder reaction to release a fluorophore. Moreover, the anticancer drug doxorubicin (Dox) covalently loaded into NCSPs is efficiently released by 1.5 MHz HIFU. Finally, an in vitro study of drug release from NCSPs is performed, demonstrating the potential of HIFU‐activated polymer mechanochemistry for sonopharmacology.

Ultrasound-Triggered Amoxicillin Release from Chitosan/Ethylene Glycol Diglycidyl Ether/Amoxicillin Hydrogels Having a Covalently Bonded Network.

An antibiotic release system triggered by ultrasound (US) was investigated using chitosan (CS)/ethylene glycol diglycidyl ether (EGDE) hydrogel carriers with amoxicillin (Amox) drug. Different CS concentrations of 1.5, 2, 2.5, and 3 wt % were gelled with EGDE and Amox was entrapped in the hydrogel carrier; the accelerated release was observed as triggered by 43 kHz US exposure at different US output powers ranging from 0 to 35 W. Among these CS hydrogel systems, the degree of accelerated Amox release depended on the CS concentration for the hydrogelation and the matrix with 2 wt % CS exhibited efficient Amox release at 35 W US power with around 19 μg/mL. The drug released with time was fitted with Higuchi and Korsmeyer-Peppas models, and the enhancement was caused by US aiding drug diffusion within the hydrogel matrix by a non-Fickian diffusion mechanism. The US effect on the viscoelasticity of the hydrogel matrix indicated that the matrix became somewhat softened by the US exposure to the dense hydrogels for 2.5 and 3% CS/EGDE, while the degree of softening was slightly marked in the CS/EGDE hydrogels prepared with 1.5 and 2% CS concentration. Such US softening also aided drug diffusion within the hydrogel matrix, suggesting an enhanced Amox release.

Green approach for polyphenol extraction from waste tea biomass: Single and hybrid application of conventional and ultrasound-assisted extraction

Based on a green approach, the potential use of waste tea biomass (fiber and second sieving) with rich polyphenol content was investigated as an alternative source of polyphenol to achieve an economic added value. In addition, this study demonstrated a comparative approach to explore the most sustainable green extraction method by the assessment of single ultrasound-assisted extraction (UAE) at various frequencies (20, 35, and 200 kHz) and the hybrid operations of ultrasound (US) and thermal extraction (50 °C and 80 °C). As a result, it has been determined that waste tea biomass, with a polyphenol extraction rate of more than 80%, provides a higher recovery capacity than tea leaf (the highest polyphenol recovery rate of 72.5%) in almost all single operations. Among the single UAE, 20 kHz was expressed as the method succeeding with high recovery rates (84%) within 30 min for fiber waste. In contrast, the hybrid operation consisting of 20 kHz US (20 min) with heating at 80 °C (10 min) yielded the highest extraction efficiency with 92% in the same time interval more economically for second sieving waste tea biomass. Therefore, this study has shown that it is possible to utilize UAE alone or in combination with heat extraction from tea waste for environmentally friendly, rapid, and effective polyphenol extraction.

Integrated sonophotocatalytic oxidation and SBR processes for the effective treatment of antibiotics with an emphasis on process optimization and microbial diversity

The presence of antibiotics in water and wastewater is a serious environmental concern of emerging interest globally. Their effective removal with a single treatment process is, however, challenging because of their widely varying characteristics, and requires innovative integration of treatment processes. In this study, sonophotocatalytic oxidation process was integrated with an aerobic sequencing batch reactor (SBR) for the comprehensive removal of a mixture of antibiotics. Initially, a Central Composite Design using Response Surface Methodology was used to optimise the operational parameters, viz. ultrasound (US) frequency, pH, and catalyst dosage, for the removal of Ciprofloxacin (CPX), Amoxicillin (AMX), and Tetracycline (TTC) in simulated wastewater. An optimised condition of 20 kHz US frequency, 6.59 initial pH, and 0.94 g L−1 catalyst dosage was observed for the maximum pollutant removal. These experimental conditions were subsequently used for combined sonophotocatalysis-aerobic SBR treatment. Three SBR systems representing a control system, pre-treated system, and untreated system, were used for the study. All three systems were able to achieve an average COD and NH4+-N removal of 85 % and 98 %, respectively. Results from the biological systems concluded that an enhanced CPX and TTC removal was observed in the pre-treated system, whereas maximum AMX removal was observed in the untreated SBR system. The metagenomic analysis confirmed the significant difference of microbial species with the addition of pretreated and untreated wastewater into the biological reactor, and pretreated system having higher number of species and greater biodiversity in comparison to untreated system.

Effect of ultrasound treatment of the skin on activation of Langerhans cells and antibody production in rodents.

In this study, we investigated whether stimulating the skin with ultrasound (US) could activate Langerhans cells (LCs) - antigen-presenting cells in the epidermis and stimulate antibody production following the subcutaneous and intradermal injection of ovalbumin (OVA) in hairless rats and BALB/c mice. Three different US frequencies (20 kHz, 1, and 3 MHz) were used and the expression of langerin was monitored as a marker for the activation of LCs in the epidermal sheet. In hairless rats, the langerin signal peaked at 12 h post-US treatment and returned to control levels at 24 h. Its expression increased with increasing irradiation time, up to 20 min, and 20 kHz US induced the highest langerin expression among the three frequencies tested. These results were reproduced in BALB/c mice. When the skin was pretreated with 20 kHz US at 0.41 W/cm2 for 10 min, the production of OVA-specific immunoglobulin G1 in mice increased by 2.8- and 3.4-fold 28 days after subcutaneous or intradermal OVA injections, respectively. These findings indicate that stimulating the skin with US can trigger skin immune responses, leading to effective antigen-specific antibody production. US-assisted transdermal vaccine delivery delivers antigens to the skin and evokes an immune response, providing an effective noninvasive immunization strategy.

Study of anthocyanins as related to stability of infrared freeze-dried rose flower using novel ultrasound pretreatment

The present study was undertaken to determine the mechanism of anthocyanin content (ACNs) and related stability of infrared freeze-dried rose flowers using novel ultrasound pretreatments (20 kHz and 45 kHz for 5, 10 and 15 min). Additionally, the impact of such processing on peroxidase, polyphenol oxidase, color, moisture, total phenolic, total flavonoid, anthocyanins degradation during storage and chemical structure of infrared freeze-dried flowers was evaluated quantitatively. Peroxidase in rose flowers was effectively inactivated while polyphenol oxidase was activated very slowly (85, 95.67, 87.33, 77.67, 93, 98, 90.67%) by ultrasound pretreatment at 45 kHz and 20 kHz for 5, 10 and 15 min, respectively. The TPC and TFC of dried rose flowers pretreated by 45 kHz ultrasound (US) was also higher than that of dried roses pretreated by 20 kHz US. Moreover, US at 45 kHz was beneficial for the retention of color and anthocyanins of roses after infrared freeze-drying. In addition, US treatment at 45 kHz for 10 min resulted the lowest ACNs degradation rate during storage. These results demonstrate that US treatment at 45 kHz is a potential technique for improving the stability of anthocyanins during its processing and storage.

Use of 20 kHz Ultrasound for Microbiological Disinfection of Fresh-Cut Industry Wash Water

Use of 20 kHz Ultrasound for Microbiological Disinfection of Fresh-Cut Industry Wash Water

Ultrasound-triggered nicotine release from nicotine-loaded cellulose hydrogel

Ultrasound (US)-triggered nicotine release system in a cellulose hydrogel drug carrier was developed with three different cellulose concentrations of 0.45 wt%, 0.9 wt%, and 1.8 wt%. The nicotine-loaded cellulose hydrogels were fabricated by the phase inversion method when the nicotine and cellulose mixture in the 6 wt% LiCl/N, N-dimethylacetamide solvent was exposed to water vapor at room temperature. Nicotine was used as the medicine due to its revealed therapeutic potential for neurodegenerative diseases like Alzheimer's and Parkinson's diseases. The behavior of US-triggered nicotine release from nicotine-cellulose hydrogel was studied at 43 kHz US frequency at the changing US output powers of 0 W, 5 W, 10 W, 20 W, 30 W, and 40 W. The significant US-triggered nicotine release enhancement was noted for the hydrogels made with 0.9 wt% and 1.8 wt% cellulose loading. The matrix made with 0.9 wt% cellulose was exhibited the highest nicotine release at the 40 W US power, and differences in nicotine release at different US powers were noticeable than at 0.45 wt% and 1.8 wt% cellulose loadings. For the three cellulose hydrogel systems, the storage modulus (G′) values at the 0.01 wt% strain rate were dropped from their initial values due to the US irradiation. This reduction was proportionately decreased when the US power was increased. The deconvolution of FTIR spectra of nicotine-loaded cellulose films before and after US exposure was suggested breakage of cellulose-nicotine and cellulose-water in the matrix; thus, the stimulated nicotine release from the cellulose matrix was promoted by the US irradiation.

Integration of Ultrasound into the Development of Plant-Based Protein Hydrolysate and Its Bio-Stimulatory Effect for Growth of Wheat Grain Seedlings In Vivo.

This study was dedicated to increasing the efficiency of producing plant-based protein hydrolysate using traditional and non-traditional treatments. Low- and high frequency ultrasound (US) at different intensities were applied to corn steep liquor (CSL) at 50 °C for 30 min, and enzymatic hydrolysis was performed using industrially produced alkaline protease. The efficiency of US and enzymatic treatments was characterized by protein solubility (soluble protein (SP) content, hydrolyzed protein (HP) concentration, and free amino acid (FAA) profile) and kinetic parameters: Michaelis–Menten constant (KM) and apparent breakdown rate constant (kA). A significant effect of 37 kHz US pre-treatment for CSL enzymatic hydrolysis was found and resulted in the highest HP concentration (17.5 g/L) using the lowest enzyme concentration (2.1 g/L) and the shortest hydrolysis time (60 min). By using US pre-treatment, on average, a 2.2 times higher FAA content could be achieved compared to traditional hydrolysis. Additionally, results for the kinetic parameters kM and kA confirmed the potential of applying US treatment before hydrolysis. The effect of CSL protein hydrolysate on plant growth was tested in vivo on wheat grain seed germination and resulted in the significant increase in germination parameters compared to the control treatment. These findings indicate that by-products of starch industry could be a promising source for the production of low-cost sustainable biostimulants.

Control of Listeria monocytogenes and Escherichia coli O157:H7 in enoki mushrooms (Flammulina velutipes) by combined treatments with organic acids, nisin, and ultrasound

This study was undertaken to evaluate the combined effect of organic acids, nisin, and ultrasound (US) on the reduction of Listeria monocytogenes and Escherichia coli O157:H7 in enoki mushrooms stored at 5 °C. Each bacterium was treated with 1–3% organic acids, 0.1% nisin, and 40 kHz US in enoki mushrooms individually or in combination at 25 °C for ≤30 min. Lactic acid (LA) was superior to malic acid, acetic acid, and nisin, decreasing L. monocytogenes and E. coli O157:H7 in enoki mushrooms by more than 3.00 log CFU/g at 25 °C for 30 min. US alone failed to inactivate L. monocytogenes and E. coli O157:H7 in enoki mushrooms. Particularly, L. monocytogenes and E. coli O157:H7 diminished to below the detection limits (<1.55 log CFU/g) in enoki mushrooms after dipping in 3% LA and 0.1% nisin incorporated with 40 kHz US at 25 °C for 30 min and remained constant incapable of growing in samples stored at 5 °C for 120 h. A transmission electron microscopy analysis presented collapse of peripheral cytoplasm layers with formation of local pores and breakage of cell wall structures in L. monocytogenes exposed to combined treatments with 3% LA, 0.1% nisin, and 40 kHz US. A combination of LA, nisin, and US exerted strong synergistic effects for controlling the mushroom carriage of L. monocytogenes and E. coli O157:H7.

Decontamination of Listeria monocytogenes in king oyster mushrooms (Pleurotus eryngii) by combined treatments with organic acids, nisin, and ultrasound

This study was conducted to evaluate the effectiveness of 1–3% organic acids alone or in combination with 0.1% nisin and/or 40 kHz ultrasound (US) on the reduction of Listeria monocytogenes in king oyster mushrooms stored at 25 °C. While L. monocytogenes in king oyster mushrooms was reduced by 3.01 log CFU g−1 after dipping in 3% malic acid (MA) at 25 °C for 30 min, the application of 40 kHz US and 0.1% nisin resulted in 0.30 and 2.25 log-reductions, respectively. When L. monocytogenes in mushrooms was subjected to a combined treatment with 3% MA, 0.1% nisin, and 40 kHz US at 25 °C for 20–30 min, this bacterium dropped to <1.48 log CFU g−1, leading to a significant collapse in the cytoplasm with formation of intracellular ruptures and breakage of cell membranes. Synergistic effect of MA-nisin dipping incorporated with US was observed in a food model.

Multilayer Microcapsules with Shell-Chelated 89Zr for PET Imaging and Controlled Delivery

Radionuclide-functionalized drug delivery vehicles capable of being imaged via positron emission tomography (PET) are of increasing interest in the biomedical field as they can reveal the in vivo behavior of encapsulated therapeutics with high sensitivity. However, the majority of current PET-guided theranostic agents suffer from poor retention of radiometal over time, low drug loading capacities, and time-limited PET imaging capability. To overcome these challenges, we have developed hollow microcapsules with a thin (<100 nm) multilayer shell as advanced theranostic delivery systems for multiday PET tracking in vivo. The 3 μm capsules were fabricated via the aqueous multilayer assembly of a natural antioxidant, tannic acid (TA), and a poly(N-vinylpyrrolidone) (PVPON) copolymer containing monomer units functionalized with deferoxamine (DFO) to chelate the 89Zr radionuclide, which has a half-life of 3.3 days. We have found using radiochromatography that (TA/PVPON-DFO)6 capsules retained on average 17% more 89Zr than their (TA/PVPON)6 counterparts, which suggests that the covalent attachment of the DFO to PVPON provides stable 89Zr chelation. In vivo PET imaging studies performed in mice demonstrated that excellent stability and imaging contrast were still present 7 days postinjection. Animal biodistribution analyses showed that capsules primarily accumulated in the spleen, liver, and lungs with negligible accumulation in the femur, with the latter confirming the stable binding of the radiotracer to the capsule walls. The application of therapeutic ultrasound (US) (60 s of 20 kHz US at 120 W cm-2) to Zr-functionalized capsules could release the hydrophilic anticancer drug doxorubicin from the capsules in the therapeutic amounts. Polymeric capsules with the capability of extended in vivo PET-based tracking and US-induced drug release provide an advanced platform for development of precision-targeted therapeutic carriers and could aid in the development of more effective drug delivery systems.

Soil Recycling Geopolymers Fabricated from High Power Ultrasound Treated Soil Slurry in the Presence of Ammonia

Soil slurry was recycled to prepare a geopolymer after treatment with high-power ultrasound (US) in the presence of NH3, HCl, and NaOH. Under 28 kHz US, 0.1 M NH3 additives effectively decarbonized the slurry, eliminating 72.2% of the carbon content from the original soil. The US-treated soils were used as raw materials for the geopolymer, as they contained Si and Al components in the range of 25–30 and 8–10 wt.%, respectively. The geopolymer was prepared with a Na2SiO4/NaOH aqueous solution at a ¼ weight ratio at 80 °C for 24 h. The resultant geopolymers from the NH3-treated soil showed the best compressive strength of 3 MPa after 1 day of curing, with a low carbon content, when NH3 was used as an additive as opposed to HCl and NaOH under 1200 US exposure.

The effect of 220 kHz insonation scheme on rt-PA thrombolytic efficacy in vitro

Ultrasound-enhanced recombinant tissue plasminogen activator (rt-PA) thrombolysis is under development as an adjuvant to ischemic stroke therapy. The goal of this study was to design a pulsed ultrasound (US) exposure scheme that reduced intracranial constructive interference and tissue heating, and maintained thrombolytic efficacy relative to continuous wave (CW) insonation. Three 220 kHz US schemes were evaluated, two pulsed insonation schemes (15 cycles, 68 µs pulse duration, 33% or 62.5% duty cycle) and an intermittent CW insonation scheme (50 s active, 30 s quiescent) over a 30-min treatment period. An in silico study using a finite-difference model of transcranial US propagation was performed to estimate the intracranial acoustic field and temperature rise in the skull for each insonation scheme. In vitro measurements with flow were performed to assess thrombolysis using time-lapse microscopy. Intracranial constructive interference was not reduced with pulsed US using a pulse length of 15 cycles compared to intermittent CW US. The 33.3% duty cycle pulsed US scheme reduced heating in the temporal bone as much as 60% relative to the intermittent CW scheme. All insonation schemes promoted sustained stable cavitation in vitro and augmented thrombolysis compared to rt-PA alone (p < 0.05). Ultraharmonic (UH) and harmonic cumulative energy over a 30 min treatment period was significantly higher (p < 0.05) for the intermittent CW US scheme compared to either pulsed US scheme. Despite the difference in cavitation emissions, no difference was observed in the clot lysis between the three US schemes. These findings demonstrate that a 33.3% duty cycle pulsed US scheme with a 15-cycle burst can reduce bone heating and achieve equivalent thrombolytic efficacy as an intermittent CW scheme.

Comparison of Different Bacteria Inactivation by a Novel Continuous-Flow Ultrasound/Chlorination Water Treatment System in a Pilot Scale

The existing traditional drinking water disinfection technology relies mainly on chlorine disinfection alone, which has high disinfection efficiency and can effectively inactivate most of the microorganisms in the water. However, it produces a series of harmful disinfection by-products (DBPs). Therefore, it is very necessary to study an efficient and environmentally friendly disinfection technology for drinking water. For this purpose, a novel continuous-flow ultrasound (US)/chlorination water treatment system was designed and developed. Escherichia coli (E. coli), Bacillus subtilis (B. subtilis), and Staphylococcus aureus (S. aureus) were selected as indicators of water treatment effects to (1) investigate the disinfection effects of different bacteria by US treatment alone at different single or dual frequencies; (2) explore the disinfection effects of US pretreatment with 8 mg/L NaClO on different bacteria to assess the promoting effects of US pretreatment; and (3) identify the optimum system process to satisfy the national standard for drinking water quality. Results showed that the dual-frequency US had better inactivation effects compared with single-frequency US, although it could not achieve an ideal disinfection level (complete disinfection). Further, 17 + 33 kHz dual-frequency US pretreatment had obvious enhancement of the disinfection efficiency, where 3.85 (E. coli), 3.65 (S. aureus), and 3.52 (B. subtilis) log reduction were achieved when 8 mg/L NaClO disinfection lasted 10 min, and finally all three bacteria achieved 4 log reduction after 30 min. Moreover, the treated water satisfied the Chinese national standard for drinking water quality, in which the residual chlorine concentration was below 4 mg/L. The utilization efficiency of disinfectant was improved approximately 40% by 17 + 33 kHz US pretreatment.

Spiral computed tomography evaluation of rabbit VX2 hepatic tumors treated with 20 kHz ultrasound and microbubbles

The aim of the present study was to explore the therapeutic effect of 20 kHz ultrasound (US) and microbubbles (MBs) on rabbit VX2 liver tumors by spiral computed tomography (CT) scanning. A total of 16 New Zealand rabbits with hepatic VX2 tumors were divided into four groups: Control, MB, low-frequency US and US + MB. The treatment effect was evaluated by spiral CT scanning prior to, during and following treatment (at 0 weeks and the end of 1 and 2 weeks). The tumor growth rate was recorded. The specimens of VX2 tumors were collected for histological examination and transmission electron microscopy (TEM). No significant differences were observed between tumor areas measured by CT and pathology after 2-week treatment (P>0.05). The mean tumor growth rates in the control, MB, US and US + MB groups after 2 weeks of treatment were 385±21, 353±12, 302±14 and 154±9%, respectively (P<0.05, US + MB vs. the other three groups). Hematoxylin and eosin staining in the US + MB group revealed coagulation necrosis, interstitial hemorrhage and intravascular thrombosis. In the control, MB and US groups, tumor cells exhibited clear nuclear hyperchromatism. TEM of US + MB revealed vascular endothelial cell wall rupture, widened endothelial cell gaps, interstitial erythrocyte leakage and microvascular thrombosis, while intact vascular endothelial cells and normal erythrocytes in the tumor vessels were observed in the control, MB and US groups. A combination of 20 kHz US and MBs may effectively inhibit rabbit VX2 tumors. Spiral CT scanning is an ideal method to evaluate the US treatment on rabbit tumors.

Acoustic field and convection pattern within liquid material during ultrasonic processing

When ultrasound propagates in a liquid alloy, nonlinear effect takes place such as cavitation effect and acoustic streaming, which accelerates the solute and thermal transportation during alloy solidification, and consequently, improves the solidification microstructures and mechanical properties of the metallic alloy. Therefore, it is significant to investigate the ultrasound propagation characteristics in liquid. Here, by choosing water as a model transparent material, the acoustic fields and flow fields induced by 20 and 490 kHz ultrasounds are investigated by numerical simulation, and the effects of frequency and ultrasonic horn radius are studied. Firstly, the simulation results demonstrate that the sound pressure under 20 kHz ultrasound decreases obviously along the ultrasonic propagation direction, and the maximum of sound pressure value is equal to the initial pressure. In this case, the cavitation effect only occurs in the region close to the ultrasonic horn. By contrast, when the ultrasonic frequency increases to 490 kHz, the sound pressure is higher than that of 20 kHz ultrasound, and displays periodical vibration characteristic along the wave propagation direction. The cavitation volume correspondingly expands to a large extent with a regular striped distribution. It can also be found that increasing the ultrasonic horn radius under 20 and 490 kHz ultrasounds can effectively promote the sound pressure level in water, and hence leads to the remarkable enlargement of cavitation volume. Secondly, the calculated results of flow field indicate that the streamlines in water are similar under the two ultrasounds with different frequencies. A jet produced by the center of horn spreads down and divergences to both sides after reaching the bottom. For both frequencies as the horn radius increases, the radius of jet increases and the average velocity in water first increases and then decreases, whose maximum value appears when the horn radius is 40 mm. Meanwhile, the average velocity under 20 kHz ultrasound is larger than that under 490 kHz ultrasound for each horn radius. Finally, particle image velocimetry method is employed to measure the velocity field in water. Both the positions of eddy and the velocity distribution are the same as the simulation results, which verifies the reliability of the present theoretical calculation model. The scenario in this work is analogous to the acoustic field and the flow field in liquid alloy, which is beneficial for the design of parameter optimization during ultrasonic processing in alloy solidification.

Timesaving Washing of Textiles Utilizing 38 kHz Ultrasound

AbstractFrom the viewpoints of prevention of garment damage as well as saving of electric energy and processing time in laundering, the possibility of time reduction by applying ultrasound has been discussed. The shake washing of polyester and cotton fabrics soiled with model oily and/or particulate contaminants together with their original fabrics was performed in an aqueous sodium dodecyl sulfate solution (SDS) without and with ultrasound. The soil removal and redeposition were evaluated from the change in the Kubelka-Munk function of the soiled and non-soiled fabric surfaces due to the washing. In the presence of ultrasound, the removal of oleic acid and carbon black from the polyester spun fabric was remarkably promoted, especially in the initial stage of the washing process. On the other hand, the soil redeposition gradually increased with wash time, especially for carbon black. Therefore, the removal and redeposition were compared between wash time 1 min and 5 min using some soils and fabrics. The soil removal was slightly larger for the 5 min-washing, whereas the soil redeposition was considerably prevented for the 1 min-washing. Comparing with a conventional washing with a drum type washer, the 1 min-shake/ultrasound combination washing had little impact damage in spite of having similar detergency power.

Treatment of hepatic carcinoma by low-frequency ultrasound and microbubbles: A case report.

In vitro and in vivo studies have identified that low-frequency ultrasound (US) and microbubbles (MBs) mediate tumor inhibitory effects. However, the application of US in the clinical setting remains unclear. The aim of the present study was to investigate the clinically therapeutic effect of 20 kHz US in combination with MBs for the treatment of hepatic carcinoma. A 71-year-old male with a hepatic malignant tumor was admitted to Nantong University Affiliated Nantong Tumor Hospital (Nantong, China). The patient was subsequently sonicated with 20 kHz US and MBs over a period of five days. The low-frequency US parameters were set at 20 kHz, 2 W/cm2, duty cycle 40% (on 2 sec, off 3 sec) for a duration of 5 min each day for a total of five days. Computed tomography (CT), contrast-enhanced US (CEUS) and carbohydrate antigen 19-9 (CA19-9) tests were performed to evaluate the therapeutic effects. Although the tumor size increased marginally on CT from 5.4 to 5.6 cm after US treatment, the intensity and enhanced-areas on the CT scans and CEUS decreased. The abdominal lymph node decreased in size, from 2.2 to 1.9 cm, and CA19-9 levels decreased from the pretreatment value of 2,007 to 734 U/ml. Therapy with low-frequency US combined with MBs may exhibit an antivasculature effect and may be used as a palliative treatment for patients with unresectable hepatic malignant tumors.

Effect of ultrasound on the aqueous viscosity of several water-soluble polymers

The effect of ultrasound (US) on the shear viscosity of water-soluble polymers, polyvinyl alcohol, polyethylene glycol and polyacrylic acid, was studied in aqueous solutions with US exposure of 23, 43, 96 and 141 kHz for an 8.5–9 W US. The US exposure significantly decreased the shear viscosity of the solutions in this order: 43>23>96>141 kHz. When US exposure was stopped, the shear viscosities of the aqueous polymer solutions reverted to their original values. US stimulation during the decrease in viscosity was supported by the ultrasonic power transmitted through the aqueous polymer solution. In addition, Fourier-transform infrared spectra obtained during US exposure showed that hydrogen bonds in the aqueous polymer solution could be broken, especially at 43 kHz. We concluded that US exposure influenced hydrogen-bond interactions between the OH groups of the polymer and water molecules in the aqueous medium. This finding was supported by US absorption of the aqueous polymer solution in the transmittance model, which shows the US absorptivity, ɛUS, for each polymer system. The effect of US on the shear viscosity of water-soluble polymer solutions was studied. The 43 kHz US significantly decreased the shear viscosity of the solutions. FT-IR spectra obtained during the US exposure showed that hydrogen bonds in the aqueous polymer solution could be broken. US absorption model was proposed to explain US absorption and the breakage in the hydrogen bonds. US effect influenced the condition of water solvation to the water-soluble polymers.