kHz Frame Rate Research Articles

EventLFM: event camera integrated Fourier light field microscopy for ultrafast 3D imaging

Ultrafast 3D imaging is indispensable for visualizing complex and dynamic biological processes. Conventional scanning-based techniques necessitate an inherent trade-off between acquisition speed and space-bandwidth product (SBP). Emerging single-shot 3D wide-field techniques offer a promising alternative but are bottlenecked by the synchronous readout constraints of conventional CMOS systems, thus restricting data throughput to maintain high SBP at limited frame rates. To address this, we introduce EventLFM, a straightforward and cost-effective system that overcomes these challenges by integrating an event camera with Fourier light field microscopy (LFM), a state-of-the-art single-shot 3D wide-field imaging technique. The event camera operates on a novel asynchronous readout architecture, thereby bypassing the frame rate limitations inherent to conventional CMOS systems. We further develop a simple and robust event-driven LFM reconstruction algorithm that can reliably reconstruct 3D dynamics from the unique spatiotemporal measurements captured by EventLFM. Experimental results demonstrate that EventLFM can robustly reconstruct fast-moving and rapidly blinking 3D fluorescent samples at kHz frame rates. Furthermore, we highlight EventLFM’s capability for imaging of blinking neuronal signals in scattering mouse brain tissues and 3D tracking of GFP-labeled neurons in freely moving C. elegans. We believe that the combined ultrafast speed and large 3D SBP offered by EventLFM may open up new possibilities across many biomedical applications.

High-frame rate, large-depth-range structured light projector based on the step-designed LED chips array.

Among numerous mature optical 3D measurement techniques, phase-shift profilometry (PSP) has been widely used because of its high precision and insensitivity to ambient light, and high-speed PSP has become a research hotspot in recent years. Current mainstream high-frame rate PSP projection techniques employ binary defocusing projection schemes, which limit the available measurement depth. We propose a high-frame rate, large-depth-range sinusoidal fringe projection technique based on step-designed LED chips array. In principle, on the one hand, the LED chips array still produces a binary pattern, so high-frame rate switching can be achieved, on the other hand, whether focusing or defocusing can generate sinusoidal fringes, avoiding the limit of defocusing projection on the depth range of measurement. A PSP projector is designed and manufactured, and 3D reconstruction of static human face mask and dynamic rotating fan is carried out at 1 kHz frame rate. In another experiment, the PSP projector projected the fringes at a 100kHz frame rate and detected the fringes with a single point photodetector, and the output waveform showed that the projection technique had the potential to be much higher than the 100 kHz frame rate. These results show that the PSP projection technology has the advantages of high-frame rate and large-depth-range, and is very useful for three-dimensional measurement of moving targets.

High-Speed Fluorescence Imaging Corroborates Biological Data on the Influence of Different Nozzle Types on Cell Spray Viability and Formation

Treating severe dermal disruptions often presents significant challenges. Recent advancements have explored biological cell sprays as a promising treatment, but their success hinges on efficient cell delivery and complete wound coverage. This requires a good spray distribution with a small droplet size, high particle number, and ample surface coverage. The type of nozzle used with the spray device can impact these parameters. To evaluate the influence of different nozzles on spray characteristics, we compared air-assisted and unassisted nozzles. The unassisted nozzle displayed small particle size, high particle number, good overall coverage, high cell viability, preserved cell metabolic activity, and low cytotoxicity. Air-assisted nozzles did not perform well regarding cell viability and metabolic activity. Flow visualization analysis comparing two different unassisted nozzles using high-speed imaging (100 kHz frame rate) revealed a tulip-shaped spray pattern, indicating optimal spray distribution. High-speed imaging showed differences between the unassisted nozzles. One unassisted nozzle displayed a bi-modal distribution of the droplet diameter while the other unassisted nozzle displayed a mono-modal distribution. These findings demonstrate the critical role of nozzle selection in successful cell delivery. A high-quality, certified nozzle manufactured for human application omits the need for an air-assisted nozzle and provides a simple system to use with similar or better performance characteristics than those of an air-assisted system.

Imaging and characterization of optical emission from ex vivo tissue during conventional and UHDR PBS proton therapy

Objective. Imaging of optical photons emitted from tissue during radiotherapy is a promising technique for real-time visualization of treatment delivery, offering applications in dose verification, treatment monitoring, and retrospective treatment plan comparison. This research aims to explore the feasibility of intensified imaging of tissue luminescence during proton therapy (PT), under both conventional and ultra-high dose rate (UHDR) conditions. Approach. Conventional and UHDR pencil beam scanning (PBS) PT irradiation of fresh ex vivo porcine tissue and tissue-mimicking plastic phantom was imaged using intensified complementary metal-oxide-semiconductor(CMOS) cameras. The optical emission from tissue was characterized during conventional irradiation using both blue and red-sensitive intensifiers to ensure adequate spectral coverage. Spectral characterization was performed using bandpass filters between the lens and sensor. Imaging of conventional proton fields (240 MeV, 10 nA) was performed at 100 Hz frame rate, while UHDR PBS proton delivery (250 MeV, 99 nA) was recorded at 1 kHz frame rate. Dependence of optical emission yield on proton energy was studied using an optical tissue-mimicking plastic phantom and a range shifter. Finally, we demonstrated fast beam tracking capability of fast camera towards in vivo monitoring of FLASH PT. Main results. Under conventional treatment dose rates optical emission was imaged with single spot resolution. Spot profiles were found to agree with the treatment planning system calculation within >90% for all spectral bands and spot intensity was found to vary with spectral filtration. The resultant polychromatic emission presented a maximum intensity at 650 nm and decreasing signal at lower wavelengths, which is consistent with expected attenuation patterns of high fat and muscle tissue. For UHDR beam imaging, optical yield increased with higher proton energy. Imaging at 1 kHz allowed continuous monitoring of delivery during porcine tissue irradiation, with clear identification of individual dwell positions. The number of dwell positions matched the treatment plan in total and per row showing adequate temporal capability of iCMOS imaging. Significance. For the first time, this study characterizes optical emission from tissue during PT and demonstrates our capability of fast optical tracking of pencil proton beam on the tissue anatomy in both conventional and UHDR setting. Similar to the Cherenkov imaging in radiotherapy, this imaging modality could enable a seamless, independent validation of PT treatments.

Multiscale drainage dynamics with Haines jumps monitored by stroboscopic 4D X-ray microscopy.

Slow multiphase flow in porous media is intriguing because its underlying dynamics is almost deterministic, yet depends on a hierarchy of spatiotemporal processes. There has been great progress in the experimental study of such multiphase flows, but three-dimensional (3D) microscopy methods probing the pore-scale fluid dynamics with millisecond resolution have been lacking. Yet, it is precisely at these length and time scales that the crucial pore-filling events known as Haines jumps take place. Here, we report four-dimensional (4D) (3D + time) observations of multiphase flow in a consolidated porous medium as captured in situ by stroboscopic X-ray micro-tomography. With a total duration of 6.5 s and 2 kHz frame rate, our experiments provide unprecedented access to the multiscale liquid dynamics. Our tomography strategy relies on the fact that Haines jumps, although irregularly spaced in time, are almost deterministic, and therefore repeatable during imbibition-drainage cycling. We studied the time-dependent flow pattern in a porous medium consisting of sintered glass shards. Exploiting the repeatability, we could combine the radiographic projections recorded under different angles during successive cycles into a 3D movie, allowing us to reconstruct pore-scale events, such as Haines jumps, with a spatiotemporal resolution that is two orders of magnitude higher than was hitherto possible. This high resolution allows us to explore the detailed interfacial dynamics during drainage, including fluid-front displacements and velocities. Our experimental approach opens the way to the study of fast, yet deterministic mesoscopic processes also other than flow in porous media.

Shear and Scholte wave measurements on surficial intertidal mudflat sediments using medical sonoelastography techniques

In low-energy environments, the water–sediment interface often contains a dynamic fluffy transition layer (FTL) of suspended organic and inorganic matter, which is transformed by complex physical, biological, and chemical processes. The FTL mechanical properties are important for understanding carbon cycle dynamics and for robust sediment acoustic characterization, but erodibility and size make it difficult to study non-invasively and at sufficient resolution. In medical ultrasound sonoelastography, shear waves are excited within the body either using the acoustic radiation force (ARF) or via external vibration at the skin surface. Sub-micrometer displacements are measured remotely by subsequent ultrasound imaging at up to 10 kHz frame rate with approximately 0.5 μm spatial resolution. In this study, sediment cores were extracted from the intertidal mudflats from the Great Bay Estuary, USA. Compressional wave speed and attenuation measurements were performed using a core logger. Shear and Scholte waves were excited in the FTL using ARF- and external-vibration-based techniques and the motion detected with clinical ultrasound imaging arrays. Upward refraction of the shear wave was observed, and shear and Scholte wave speed and attenuation were measured. Finally, scanning electron microscopy was used to image the microstructure. Comparison between wave properties and microstructure will be discussed.

Contrast-Enhanced Imaging of Histotripsy Bubble Clouds Using Chirp-Coded Excitation and Volterra Filtering.

Histotripsy is a focused ultrasound therapy that ablates tissue via bubble cloud activity. Real-time ultrasound image guidance is used to ensure safe and effective treatment. Plane-wave imaging enables tracking of histotripsy bubble clouds at a high frame rate but lacks adequate contrast. Furthermore, bubble cloud hyperechogenicity is reduced in abdominal targets, making the development of contrast-specific sequences for deep-seated targets an active area of research. Chirp-coded subharmonic imaging was reported previously to enhance histotripsy bubble cloud detection by a modest 4-6 dB compared to the conventional sequence. Incorporating additional steps into the signal processing pipeline could enhance bubble cloud detection and tracking. In this study, we evaluated the feasibility of combining chirp-coded subharmonic imaging with Volterra filtering for enhancing bubble cloud detection in vitro. Chirped imaging pulses were used to track bubble clouds generated in scattering phantoms at a 1-kHz frame rate. Fundamental and subharmonic matched filters were applied to the received radio frequency signals, followed by a tuned Volterra filter to extract bubble-specific signatures. For subharmonic imaging, the application of the quadratic Volterra filter improved the contrast-to-tissue ratio from 5.18 ± 1.29 to 10.90 ± 3.76 dB, relative to the application of the subharmonic matched filter. These findings demonstrate the utility of the Volterra filter for histotripsy image guidance.

Backscattering amplitude in ultrasound localization microscopy

In the last decade, Ultrafast ultrasound localisation microscopy has taken non-invasive deep vascular imaging down to the microscopic level. By imaging diluted suspensions of circulating microbubbles in the blood stream at kHz frame rate and localizing the center of their individual point spread function with a sub-resolution precision, it enabled to break the unvanquished trade-off between depth of imaging and resolution by microscopically mapping the microbubbles flux and velocities deep into tissue. However, ULM also suffers limitations. Many small vessels are not visible in the ULM images due to the noise level in areas dimly explored by the microbubbles. Moreover, as the vast majority of studies are performed using 2D imaging, quantification is limited to in-plane velocity or flux measurements which hinders the accurate velocity determination and quantification. Here we show that the backscattering amplitude of each individual microbubble can also be exploited to produce backscattering images of the vascularization with a higher sensitivity compared to conventional ULM images. By providing valuable information about the relative distance of the microbubble to the 2D imaging plane in the out-of-plane direction, backscattering ULM images introduces a physically relevant 3D rendering perception in the vascular maps. It also retrieves the missing information about the out-of-plane motion of microbubbles and provides a way to improve 3D flow and velocity quantification using 2D ULM. These results pave the way to improved visualization and quantification for 2D and 3D ULM.

Four-color fluorescence cross-correlation spectroscopy with one laser and one camera.

The diffusion and reorganization of phospholipids and membrane-associated proteins are fundamental for cellular function. Fluorescence cross-correlation spectroscopy (FCCS) measures diffusion and molecular interactions at nanomolar concentration in biological systems. We have developed an economical method to simultaneously monitor diffusion and complexation with the use of super-continuum laser and spectral deconvolution from a single detector. Customizable excitation wavelengths were chosen from the wide-band source and spectral fitting of the emitted light revealed the interactions for up to four chromatically overlapping fluorophores simultaneously. This method was applied to perform four-color FCCS that we demonstrated with polystyrene nanoparticles, lipid vesicles, and membrane-bound molecules. Up to four individually customizable excitation channels were selected from the broad-spectrum fiber laser to excite the diffusers within a diffraction-limited spot. The fluorescence emission passed through a cleanup filter and a dispersive prism prior to being collected by a sCMOS or EMCCD camera with up to 1.8 kHz frame rates. The emission intensity versus time of each fluorophore was extracted through a linear least-square fitting of each camera frame and temporally correlated via custom software. Auto- and cross-correlation functions enabled the measurement of the diffusion rates and binding partners. We have measured the induced aggregation of nanobeads and lipid vesicles in solution upon increasing the buffer salinity. Because of the adaptability of investigating four fluorophores simultaneously with a cost-effective method, this technique will have wide application for examining macromolecular complex formation in model and living systems.

Employing Surface Curvature for Spatially Resolved X‐Ray Reflectivity: Graphene Domains on Liquid Copper

AbstractHere the possibility of utilizing X‐ray reflectivity for visualization with ≈µm spatial resolution of a surface with a heterogeneous electron density due to a partial coverage by another nanometrically thin material is demonstrated. It requires the sample to be convexly bent, thus reflecting the collimated incident beam onto a magnified image recorded by a position‐sensitive detector. By the use of a small, intense, and parallel beam such as provided by the most recent synchrotron sources, one can record such spatially resolved X‐ray reflectivity with 0.1–1 kHz frame rate. The use of the method for in situ, time‐resolved characterization of single‐layer graphene domains during their chemical vapor deposition on a naturally curved surface of a liquid copper drop is demonstrated. This method can follow the growth kinetics, including the coverage ratio, 2D crystal (flake) sizes, and distances between flakes. By taking a single scan, the individual X‐ray reflectivity curves can be reconstructed, of both covered and noncovered parts of the surface, allowing to deduce the corresponding electron density profiles perpendicular to the surface. The technique has a promising perspective for in situ study of 2D materials, ultrathin films, and self‐assemblies on liquid as well as solid surfaces.

Flame Dynamics in the Combustion Chamber of Hybrid Rocket Using Multiangle Chemiluminescence

The flame dynamics in the combustion chamber of a hybrid rocket motor were visualized using novel chemiluminescence imaging. A multidirectional visualization system employing endoscopes generated images based on methylidyne chemiluminescence (CH*), with one endoscope in the precombustion chamber and two in the postcombustion chamber. Images were collected with a high-speed camera using a 1 ms exposure and a 1 kHz frame rate. Fuel grains having a helical or a conventional circular port structures were assessed, and combustion trials were conducted using a laboratory-scale hybrid rocket motor with oxygen as the oxidizer at mass flow rates from 10.43 to : equivalent to combustion chamber pressures ranging from 0.7 to 1.24 MPa. Flame structures were observed during the ignition, combustion, and shutdown stages; and the helical grain generated a larger, more intense flame zone. A proper orthogonal decomposition analysis showed that the helical grains also produced a greater degree of turbulence and stronger oscillations. These results confirm that a helical structure increases the flow turbulence and convective heat transfer in the combustion chamber. These effects lead to higher regression rates and better mixing efficiency that may, in turn, provide greater combustion efficiency at optimized oxidizer/fuel ratios.

Ultra-fast, high spatial resolution single-pulse scintillation imaging of synchrocyclotron pencil beam scanning proton delivery

Objective. To demonstrates the ability of an ultra-fast imaging system to measure high resolution spatial and temporal beam characteristics of a synchrocyclotron proton pencil beam scanning (PBS) system. Approach. An ultra-fast (1 kHz frame rate), intensified CMOS camera was triggered by a scintillation sheet coupled to a remote trigger unit for beam on detection. The camera was calibrated using the linear (R 2 > 0.9922) dose response of a single spot beam to varying currents. Film taken for the single spot beam was used to produce a scintillation intensity to absolute dose calibration. Main results. Spatial alignment was confirmed with the film, where the x and y-profiles of the single spot cumulative image agreed within 1 mm. A sample brain patient plan was analyzed to demonstrate dose and temporal accuracy for a clinically-relevant plan, through agreement within 1 mm to the planned and delivered spot locations. The cumulative dose agreed with the planned dose with a gamma passing rate of 97.5% (2 mm/3%, 10% dose threshold). Significance. This is the first system able to capture single-pulse spatial and temporal information for the unique pulse structure of a synchrocyclotron PBS systems at conventional dose rates, enabled by the ultra-fast sampling frame rate of this camera. This study indicates that, with continued camera development and testing, target applications in clinical and FLASH proton beam characterization and validation are possible.

Jungfraujoch: hardware-accelerated data-acquisition system for kilohertz pixel-array X-ray detectors.

The JUNGFRAU 4-megapixel (4M) charge-integrating pixel-array detector, when operated at a full 2 kHz frame rate, streams data at a rate of 17 GB s-1. To operate this detector for macromolecular crystallography beamlines, a data-acquisition system called Jungfraujoch was developed. The system, running on a single server with field-programmable gate arrays and general-purpose graphics processing units, is capable of handling data produced by the JUNGFRAU 4M detector, including conversion of raw pixel readout to photon counts, compression and on-the-fly spot finding. It was also demonstrated that 30 GB s-1 can be handled in performance tests, indicating that the operation of even larger and faster detectors will be achievable in the future. The source code is available from a public repository.

Analysis of liquid surface deformation and breakups using three-dimensional high-speed data recorded with a single camera

Analyzing the deformation of liquid surfaces to better understand, for example, wave generation in oceanology or the formation of industrial spray systems, requires a series of three-dimensional snapshots that temporally resolve such events. This requirement is challenging, especially when applied to transient liquid surfaces that deform rapidly. A technique called Fringe Projection-Laser Induced Fluorescence (FP-LIF), developed by the authors, generates three-dimensional surface reconstructions of irregular liquid structures using snapshots recorded via a single camera only. In this article, FP-LIF is associated, for the first time, with a high-speed detection system, allowing the three-dimensional visualization of liquid surface deformation and breakups at a kHz frame rate. The technique is applied here at 20 kHz for imaging the complete development of a wide hollow-cone water spray and analyzing, in detail, the transition from early injection to stabilization. The three-dimensional image series covered a total time window of 300 ms (6000 frames). It is observed during the first 100 ms that the initial liquid jet deforms into a stable tulip shaped sheet. Then, between 100 and 180 ms, the tulip shape gradually grows until its stabilization corresponds to the final conical shaped sheet. Once the stabilization is reached, the fluctuation of the final spray-angle—ranging from 40° to 50°—is extracted by post-processing 1000 consecutive three-dimensional images, providing a detailed analysis of the radial symmetry of the spray over time and three-dimensional space. The results provided in this article are relevant for the validation of Computational Fluid Dynamics spray models.

A Scalable CMOS Molecular Electronics Chip for Single-Molecule Biosensing.

This work reports the first CMOS molecular electronics chip. It is configured as a biosensor, where the primary sensing element is a single molecule "molecular wire" consisting of a ∼100 GΩ, 25 nm long alpha-helical peptide integrated into a current monitoring circuit. The engineered peptide contains a central conjugation site for attachment of various probe molecules, such as DNA, proteins, enzymes, or antibodies, which program the biosensor to detect interactions with a specific target molecule. The current through the molecular wire under a dc applied voltage is monitored with millisecond temporal resolution. The detected signals are millisecond-scale, picoampere current pulses generated by each transient probe-target molecular interaction. Implemented in a 0.18 μm CMOS technology, 16k sensors are arrayed with a 20 μm pitch and read out at a 1 kHz frame rate. The resulting biosensor chip provides direct, real-time observation of the single-molecule interaction kinetics, unlike classical biosensors that measure ensemble averages of such events. This molecular electronics chip provides a platform for putting molecular biosensing "on-chip" to bring the power of semiconductor chips to diverse applications in biological research, diagnostics, sequencing, proteomics, drug discovery, and environmental monitoring.

Quantitative fluorescence-based imaging of in-cylinder fuel film thickness at kHz frame rates in a production-type direct-injection spark-ignition engine

Incomplete evaporation of liquid fuel films inside the combustion chamber is a major source of soot and particulate emissions in internal combustion engines. In this study, quantitative imaging of liquid fuel films on the cylinder wall is performed by laser-induced fluorescence (LIF) in a single cylinder engine with optical access to the cylinder liner. A four-component surrogate fuel mixed with 0.5% anisole as the fluorescent tracer was directly injected in a single injection into the motored engine. Fuel impinging on the cylinder wall is excited at 266 nm at kHz repetition rates and imaged by a high-speed camera coupled with an image intensifier. Simultaneously, lubricating oil doped with 0.01 mmol/l pyrromethene 567 is excited at 532 nm and imaged with a second camera. The fuel film thickness on the cylinder wall is compared for two different injections timings, 285 and 240°CA before top-dead center. The initial fuel film thickness is similar for both, but evaporation occurs faster for the later injection, such that the film thickness is similar at the time the piston rings reach the fuel films. When the rings scrape the fuel off the wall, it accumulates on top of the first piston ring and is partly transported downwards to the second and third ring. In the simultaneous oil image, washout of lubricating oil is seen.

Effects of injection pressure on the flame front growth in an optical direct injection spark ignition engine

• Optical diagnostics performed at wide open throttle and 1200 rpm conditions. • Elevated injection pressure does not monotonically increase engine output. • Higher spatial variation in turbulence measured for very high injection pressure. • Local overmixing and increased mixture inhomogeneity are likely causes. • Despite decreased power, soot is still lower at very high injection pressure. The fuel injection pressure of direct injection spark ignition (DISI) petrol engines has increased significantly to improve engine efficiency and reduce air-polluting emissions. The present study performs a detailed analysis of spray penetration, burn duration, flame front vectors and turbulence intensity to provide scientific explanations about the optimal injection pressure observed for the maximum power output/efficiency in a single-cylinder optically accessible DISI engine. Five injection pressures of 5, 10, 15, 20 and 25 MPa were tested based on an equal-split double injection strategy using a side-mounted six-hole direct injector. A high-speed camera with a 20 kHz frame rate was used to record spray development, propagating petrol flame and soot luminosity. The Mie-scattering technique was used to image the fuel dispersion under varied injection pressure and to calculate spray penetration. For time-resolved, two-dimensional flame front vector extraction, a newly developed flame image velocimetry (FIV) was applied to high-speed natural combustion luminosity movies. A spatial filtering method was also used for the flow decomposition to acquire flame front turbulence intensity. A total of 100 combustion cycles were FIV processed for each test pressure to tackle the inherent cyclic variations. The spray images showed that higher fuel injection pressure leads to faster spray tip penetration and enhanced spray evaporation for better air/fuel mixing. However, the in-cylinder pressure and power output do not continue to increase with an increase in injection pressure; they peak at 15 MPa and decrease at higher injection pressure. The spatially averaged flame front vector magnitude and turbulence intensity also peak at 15 MPa, indicating the optimal injection pressure was due to the maximum turbulent flame front growth. The distribution of turbulence intensity along the flame boundary shows increased variations at injection pressure higher than 15 MPa, because local over-mixing caused low turbulent flame growth regions. Although the highest turbulent flame front growth was not measured at 25 MPa, the soot luminosity was much lower than that of 15 MPa, which explains the development trend towards higher injection pressure.

Simultaneous Multiple Timescale Imaging For High-Speed Accelerometry Using The FRAME-Method

Fast transient events, such as the disintegration of liquid bodies or chemical reactions between radical species, involve various processes that may occur at different timescales. Currently, there are two alternatives for monitoring such events: burst- or high-speed imaging. Burst imaging at ultra-high speeds (~ 100 MHz - THz) allows the fastest processes to be captured but only for a narrowly confined period of time and under the condition that the onset can be predicted. To monitor long lasting events with existing high-speed imaging technology, the frame rate must be reduced (~ kHz - 1 MHz) and thus processes occurring on sub-microsecond timescales cannot be observed. To date, no imaging technology is capable of visualizing detailed sub-microsecond dynamics over a long period of time. In this manuscript we present a solution to this technological gap by combining multiplexed imaging with high-speed sensor technology resulting in temporally resolved image series at two simultaneous timescales. We demonstrate its unique capability of tracking structures at MHz burst rates over a long time by, for the first time, extracting 2D accelerometry data acting upon liquid bodies at kHz frame-rates.

Simultaneous multiple time scale imaging for kHz–MHz high-speed accelerometry

Fast transient events, such as the disintegration of liquid bodies or chemical reactions between radical species, involve various processes that may occur at different time scales. Currently, there are two alternatives for monitoring such events: burst- or high-speed imaging. Burst imaging at ultrahigh speeds ( ∼ 100 MHz to THz ) allows for the capture of nature’s fastest processes but only for a narrowly confined period of time and at a repetition rate of ∼ 10 Hz . Monitoring long lasting, rapidly evolving transient events requires a significantly higher repetition rate, which is met by existing ∼ kHz to 1 MHz high-speed imaging technology. However, the use of such systems eliminates the possibility to observe dynamics occurring on the sub-microsecond time scale. In this paper, we present a solution to this technological gap by combining multiplexed imaging with high-speed sensor technology, resulting in temporally resolved, high-spatial-resolution image series at two simultaneous time scales. We further demonstrate how the collection of such data opens up the tracking of rapidly evolving structures up to MHz burst rates over long durations, allowing, for the first time, to our knowledge, the extraction of acceleration fields acting upon the liquid bodies of an atomizing spray in two dimensions at kHz frame rates.

Dynamic phase measurement of fast liquid crystal phase modulators.

We present dynamic time-resolved measurements of a multi-pixel analog liquid crystal phase modulator driven at a 1 kHz frame rate. A heterodyne interferometer is used to interrogate two pixels independently and simultaneously, to deconvolve phase modulation with a wide bandwidth. The root mean squared optical phase error within a 30 Hz to 25 kHz bandwidth is <0.5° and the crosstalk rejection is 50 dB. Measurements are shown for a custom-built device with a flexoelectro-optic chiral nematic liquid crystal. However, the technique is applicable to many different types of optical phase modulators and spatial light modulators.