The production of seedlings of high quality becomes an important strategy for the success of a crop. The present research had the objective of evaluating the effect of different bioestimulant doses, Stimulate®, in the formation of passion fruit seedlings. The experiment was carried out in greenhouse conditions, in randomize complete blocks experimental design with six treatments and four replications, with 24 plants per plots. The treatments were constituted of different bioestimulante doses: 0 (distilled water), 4, 12, 16 and 20 mL kg-1 seeds. Plants were collected 15, 22, 29, 36, 43 and 50 days after the emergency. Evaluations were plant height, leaf area, dries mass of leaves, shoot, root and total dry mass; root/shoot relation, absolute and relative growth rate, net assimilate rate, leaf area ratio and specific leaf area. Positive effect of the bioestimulant was verified on most of the appraised variables, in relation to the control. Doses between 4 and 6 mL of Stimulate® kg-1 of seeds promoted better balance between the root and shoot, which can improve the establishment of the seedlings in the field.