Key Activities Research Articles

ANALISIS STRATEGI PENGEMBANGAN USAHA MENGGUNAKAN BUSINESS MODEL CANVAS (STUDI KASUS DI KOLAM BENIH DAN KONSUMSI PEMUDA PEMBUDIDAYA IKAN LELE CILEUNYI KABUPATEN BANDUNG)

The purpose of this research is to analyze the business model of the Cileunyi Catfish Cultivation Youth Seed and Consumption Pool using the Business Model Canvas, and analyze the development strategy of the catfish farming business at the Cileunyi Catfish Cultivation Youth Seed and Consumption Pool. This research was conducted at the Catfish Cultivation Youth Seed and Consumption Pool, on Jalan Cibiru Beet Ds. Cileunyi Wetan Kec. Cileunyi Kab. Bandung, West Java. The research method used is a case study with a quantitative and qualitative approach. Data collection was carried out by observation, questionnaires, interviews, and literature studies. Respondents were taken by purposive sampling method with a total of 31 respondents. The analysis used is business model canvas analysis, and SWOT analysis. The results of the Business Model Canvas analysis, the authors identified key elements utilizing 9 variables, namely customer segments, value propositions, channels, customer relationships, revenue streams, key resources, key activities and cost structures that contribute to the success of the catfish farming business. The results of the SWOT analysis obtained the position of the X axis (0.17) and the Y axis (0.38) are in quadrant I, which means that the strategy applied is an aggressive strategy (S-O), namely utilizing existing strengths to seize the maximum opportunity.

A Coordination Approach to Support Crowdsourced Software-Design Process

Crowdsourcing software design (CSD) is the completion of specific software-design tasks on behalf of a client by a large, unspecified group of external individuals who have the specialized knowledge required by an open call. Although current CSD platforms have provided features to improve coordination in the CSD process (such as email notifications, chat, and announcements), these features are insufficient to solve the coordination limitations. A lack of appropriate coordination support in CSD activities may cause delays and missed opportunities for participants, and thus the best quality of design contest results may not be guaranteed. This research aims to support the effective management of the CSD process through identifying the key activity dependencies among participants in CSD platforms and designing a set of process models to provide coordination support through managing this activity. In order to do this, a five-stage approach was used: First, the current CSD process was investigated by reviewing 13 CSD platforms. Second, the review resulted in the identification of 17 possible suggestions to improve CSD. These suggestions were evaluated in stage 3 through distributing a survey to 41 participants who had experience in using platforms in the field of CSD. In stage 4, we designed ten process models that could meet the requirements of suggestions, while in stage 5, we evaluated these process models through interviews with domain experts. The result shows that coordination support in the activities of the CSD can make valuable contributions to the development of CSD platforms.

Silicon-seed priming promotes seed germination under CA-induced autotoxicity by improving sucrose and respiratory metabolism in cucumber (Cucumis sativus L.)

Seed germination is one of the critical and sensitive stages of early plant growth, and its process is prevented by cinnamic acid (CA). Silicon (Si) plays a critical role in mitigating abiotic stresses and seed germination in plants, but little is known about its role in seed germination and physiology in CA-stressed cucumber. Here, we conducted experiments in the State Key Laboratory of Aridland Crop Science, Gansu Agricultural University from March to June 2021 to investigate the effects of Si-seed priming on growth, antioxidant capacity, sucrose mobilization and respiratory metabolism during germination under CA stress. Our results showed that seed soaking with Si (9 mmol/L) significantly reduced membrane lipid peroxidation and promoted post-germination growth of cucumber seeds under CA (2.0 mmol/L) stress. Si increased key enzyme activities in sucrose metabolism in CA-stressed seeds after germination, accelerating sucrose degradation and fructose synthesis. Si also enhanced the activities of key enzymes in the glycolytic pathway and pentose phosphate pathway in seeds, as well as in the post-germination tricarboxylic acid cycle, promoting glucose decomposition and ATP synthesis. Principal component analysis significantly separated the CK, Si, and CA + Si treatments from the CA treatment in the first principal component after 48 h of treatment. In addition, qRT-PCR analysis showed that Si induced overexpression of genes related to sucrose and respiratory metabolism in seeds treated with CA for 48 h. In conclusion, our findings provide evidence that Si priming may be an effective method to reverse CA inhibition of cucumber seeds, which effectively improve germination under CA stress by attenuating membrane lipid peroxidation and enhancing sucrose mobilization and respiratory metabolism in cucumber.

Development of an entrustable professional activities framework for physiotherapists working in orthopaedic triage and assessment roles in New Zealand

In New Zealand, an increasing number of physiotherapists have been engaged in orthopaedic triage and assessment (OTA) roles within elective orthopaedic departments to help optimise surgeon clinic time and improve timely access to diagnostic assessment and treatment planning for people with musculoskeletal conditions. To date no framework has been available to guide physiotherapists and surgeons in developing these roles. This commentary describes the development of an Entrustable Professional Activity (EPA) framework for physiotherapists in OTA roles in which five key clinical activities were identified. The framework defines the context, limitations, knowledge, skills, attributes, and behaviours needed for each activity. The EPAs were mapped to existing New Zealand physiotherapy competencies and key competencies identified that are needed for safe and effective practice with minimal or no supervision. This EPA framework is intended for use in elective orthopaedic departments, to support the development of physiotherapists working in orthopaedic triage and assessment roles in clinical subspecialty areas.

Differential modulation of inflammatory cytokines by recombinant IL-10 in IL-1β and TNF-α ̶ stimulated equine chondrocytes and synoviocytes: impact of washing and timing on cytokine responses

Osteoarthritis (OA) remains a challenging joint disorder necessitating effective anti-inflammatory interventions. In this study, our primary objective was to establish an in vitro protocol that replicates the clinical investigation of anti-inflammatory drugs intended for OA management. Focusing on recombinant IL-10 (r.IL-10) as a potential anti-inflammatory treatment, we designed and implemented two distinct protocols to evaluate the efficacy of r.IL-10 in modulating chondrocyte and synoviocyte inflammation.The experimental design involved sequential stimulation with IL-1β and TNF-α for 24 h, followed by washing (model 1) or not washing (model 2) the cells before r.IL-10 treatment. Samples were collected after 6–24 h of treatment. Cellular responses were evaluated by quantifying gene expression and synthesis of key inflammatory cytokines and proteases.The expression and synthesis of inflammatory cytokines and proteases was significantly affected by washing and treatment time. The expression of IL-1β, TNF-α, IL-8, MMP-13, and ADAMTS5 were effectively reduced in r.IL-10-treated chondrocytes and synoviocytes in model 2 after 24 h, particularly at concentrations of 10 and 20 ng/mL. r.IL-10 treatment significantly increased IL-6 gene expression in chondrocytes at all time points. However, in synoviocytes, IL-6 expression was significantly lower in model 2 after 24 h of r.IL-10 treatment. r.IL-10 treatment significantly decreased IL-1β and TNF-α content in synoviocyte supernatants, particularly in model 2 at concentrations of 10 and 20 ng/mL after 6 and 24 h. r.IL-10 treatment in chondrocytes led to a significant decrease in IL-1β supernatant concentrations in model 2 after 24 h only.This study demonstrated that r.IL-10 treatment effectively reduces key inflammatory markers and matrix metalloproteinase activity in both chondrocytes and synoviocytes, particularly in model 2 where cells were not washed prior to treatment. These findings highlight r.IL-10’s potential as a robust anti-inflammatory agent for OA management and suggest its critical role in developing effective therapeutic strategies for OA.

The Effects of AR-Based Physics Homework on Learning Circular Motion

Abstract In this study, we compared the effectiveness of AR-based homework, traditional homework, and mixed-approach homework in learning about circular motion. To that end, we conducted a pretest-posttest quasi-experiment involving 135 first-year students enrolled in an introductory physics course at the University of Zagreb, Faculty of Chemical Engineering and Technology, Croatia. The students in the experimental group completed augmented reality (AR)-based homework assignments. In these assignments, their learning about circular motion was supported by a meticulously designed worksheet that included four AR-supported activities. In the mixed-approach group, students were given a homework assignment that included three AR-supported activities and one quantitative textbook problem, whereas the traditional group’s homework consisted of four quantitative textbook problems covering the same content. Findings from our study suggest that the post-treatment scores for all groups were significantly higher than the pretreatment scores, with the largest pre-post gains observed in the mixed-approach group. We conclude that combining carefully selected quantitative problems with key AR activities is the most promising approach.

Delayed application of water and fertilizer increased wheat yield but did not improve quality parameters

Context or problemWheat is an important food crop for mankind, simultaneous improvement of wheat yield and quality is human most important goal, but they often cannot increase at the same time. Objective or research questionThe effects of irrigation mode, nitrogen application rate and topdressing time on yield and quality of two wheat varieties were studied, and the possibility of synergistic improvement of yield and quality was also explored. MethodsTwo irrigation treatments (conventional border irrigation and micro-sprinkling irrigation) were set up, and three nitrogen application rate treatments (120, 210 and 300kgN ha−1) were set up under each irrigation treatment. The yield formation and quality parameters of wheat were investigated. ResultsThe grain yield, key enzyme activities of protein synthesis, protein content and dough processing quality of the two cultivars increased with the increase of nitrogen application rate. However, when the nitrogen level was higher than 210kgN ha−1, the grain yield and processing quality of winter wheat did not increase further. Under the same nitrogen application rate, compared with conventional border irrigation (CBI), micro-sprinkling irrigation (MSI) significantly increased the grain yield and total protein content, but did not improve the ratio of glutenin to gliadin and dough processing quality parameters. ConclusionsUnder the experimental conditions, the synergistic improvement of wheat yield and quality parameters could be achieved by optimizing nitrogen application rate, and the delayed application of water and fertilizer could further increase wheat grain yield, but did not improve grain quality parameters. Implications or significanceThis study provided theoretical guidance for further improving the grain yield and quality of winter wheat in the North China Plain.

IL-1β mediated fibroblast-neutrophil crosstalk promotes inflammatory environment in skin lesions of SLE

Systemic lupus erythematosus (SLE) is characterized by immune dysregulation, with neutrophil infiltration in skin lesions contributing to inflammation and disease progression. However, the interaction between fibroblasts and neutrophils in SLE skin lesions has not been fully explored. Using single-cell RNA sequencing, we identified a unique CXCL1+ fibroblast subset in SLE lesions. We found that CXCL1+ fibroblasts recruit and activate neutrophils, increasing the production of inflammatory mediators, reactive oxygen species, and neutrophil extracellular traps. These fibroblasts also facilitated the transition of neutrophils to a low-density phenotype. Notably, these fibroblasts delayed neutrophil apoptosis, extending their survival and amplifying inflammation. Serum amyloid A1, secreted by CXCL1+ fibroblasts, emerged as a key activator of neutrophils. Activated neutrophils, in turn, secreted IL-1β to induce CXCL1+ fibroblast differentiation via activating the NF-κB pathway. In conclusion, our findings reveal that IL-1β-induced CXCL1+ fibroblasts significantly modulate pro-inflammatory neutrophils, underscoring the critical crosstalk between fibroblasts and neutrophils in SLE pathogenesis.

Mushroom Cultivation and Rural Development in Sivaganga District of Tamil Nadu

Mushrooms have been consumed for centuries and are recognized for their nutritional and economic potential. Their low-cost inputs, high yields, and ability to grow on agricultural waste make them an attractive agricultural venture, especially for rural communities. In Tamil Nadu, mushroom farming has seen growth due to favorable climatic conditions and increasing demand for nutritious food. The current study focuses on farm women in the Sivaganga district, particularly mushroom farmers from four self-help groups (SHGs). Using both primary and secondary data collection methods, the study evaluates these women’s role performance, awareness levels, and training needs in mushroom farming. Primary data was gathered through structured interviews, focus group discussions, and observations, while secondary sources included government reports and SHG records. The study assessed key activities such as spawn procurement, production processes, and post-harvest management, as well as the women’s access to resources and credit. Findings from the study led to the development of a multi-stakeholder, gender-sensitive entrepreneurship model for mushroom farming. This study is aims to enhance the skills and income of farm women, empowering them to become successful agripreneurs. The combined use of primary and secondary sources ensured a comprehensive analysis of the socio-economic dynamics in mushroom farming.

Review of Literature: Business Sustainability Essentials For Business Startup

This literature review discusses research related to business model innovation and startup sustainability. The review shows that business model innovation plays an important role in increasing profits and value creation, which also helps startups survive in a dynamic business environment. Sustainable business models, concepts such as a business approach that emphasizes ethical practices and accountability in addressing social, environmental, and economic impacts, consumer awareness and technological innovation play an important role. The importance of the elements of an eco-conscious approach to business operations, particularly in light of the fierce competition and significant transformations shaping the food industry. These elements include client segments, the connections and interactions a business maintains with its customers to build loyalty and trust., key activities, key resources, partner network and cost structure. With regard access to resources and activities that involve the use of innovative, distinctive, and eco-friendly raw materials, along with certain assets, can ensure high-quality products with low environmental impact

Expression, characterization and application of the deoxynivalenol dehydrogenase from Devosia yakushimensis.

Deoxynivalenol (DON) is one of the type-B trichothecene mycotoxins generally produced by Fusarium fungi and widely pollutes wheat and other grains, causing a great loss in grain yield and significant threat to human health. Enzymatic treatment of DON has displayed a significant role in controlling its levels in recent years. Here, the DON dehydrogenase from Devosia yakushimensis (DeDDH) is reported. DeDDH exhibited the highest activity in an environment of 35 °C and pH 7.5. In addition, the residual activity of DeDDH was about 40% after pre-incubation at 80 °C for 0.5 h, suggesting a good thermostability. The presence of Ca2+ significantly boosted the activity of DeDDH, but substituting Ca2+ with other ions could hardly retain the activity. At the optimal conditions, DeDDH efficiently degraded 75% DON (45 μg mL-1) within 4 h. Notably, when three cofactors, PQQ, PMS and DCPIP, were added together, more than 90% DON (45 μg mL-1) was quickly degraded in the first 40 min. The residues Thr485 and Leu521 were found to be significant to the activity of DeDDH by site-directed mutagenesis, much different from other DON dehydrogenases. Nevertheless, further research on the crystal structure determination of DeDDH, key catalytic residue identification, catalytic activity and thermostability modification still needs to be conducted for application in industry. © 2024 Society of Chemical Industry.

Practitioner’s Perspective on Activities and Outcomes of the Weight Management Program: A Qualitative Study

This study aims to explore the activities and outcomes of weight management programs from the practitioner’s perspective. A qualitative approach was employed in this study, which included in-depth interview and document analysis. Ten practitioners with experience and diverse backgrounds were interviewed using a semi-structured questionnaire. Concurrently, document analsis was utilised by examining the printed and online documents provided by the practitioners. The verbatim transcripts and documents were analysed thematically by using Nvivo 12 software. Thematic analysis revealed four themes that represent the key activities generally executed in weight management programs: 1) nutrition and physical activities; 2) screening and monitoring; 3) motivation and spirituality, and 4) task and challenge. This study found two uncommon activities rarely mentioned in previous programs: spirituality and challenge. Practitioners also highlighted activities in the program had positive effects on participants, including: 1) demonstrating positive selfchange; 2) influencing their surroundings, and 3) cost savings. Notably, combining these activities could lead to improved outcomes, particularly when participants are fully committed to the program. In conclusion, this study identified four primary activities and three positive outcomes from the weight management programs. From the practitioner’s perspective, integrating these activities was seen as a good strategy to achieve an optimal result. Hence, it is recommended that future practitioners to incorporate and diversify the activities to enhance their effectiveness.

Sustainability of Rural Electrification Projects in Developing Countries

This study provides a review of the sustainability of rural electrification projects in developing countries to identify drivers of the long-term success of these initiatives. Unlike previous studies that often separate sustainability into social, economic, and environmental dimensions, this research adopts a comprehensive approach, acknowledging the interconnectedness of these aspects. Twenty-one sustainability drivers were identified. Based on these, a framework was proposed to organize them across project phases and key activities, supporting sustainable project planning and execution. This framework enables stakeholders to integrate sustainability considerations into the design and management of rural electrification projects, facilitating strategic action across diverse areas. The findings underscore well-known sustainability drivers such as renewable energy adoption while highlighting less-researched aspects, including project monitoring and waste management. Further studies should explore these under-researched areas and examine the potential of digitalization to enhance project sustainability, particularly as rural connectivity improves in developing countries. This approach can provide insights into optimizing rural electrification projects for long-term success and resilience.

An Integrated Model for Pollution Prevention, Ecological Integration and Sustainability of Surface Water: Case of Awetu River, Jimma City, South-Western Ethiopia

There are complex relationships among water quality, ecological integration and sustainability of surface water resource. Several factors are interacting in these three things, making them complex for understanding and managing, while the integration of these three is very important for sustainable development, environmental sustainability and public health. To increase the understanding of this complex relationship, appropriate tool is needed. Modeling is considered as an appropriate tool to simplify the understanding of this concept. The objective of this study is developing an integrated conceptual model for pollution prevention, ecological integration and sustainability of Awetu River basin. To develop this model, a preliminary study was conducted in the study area, which includes water quality status, its ecological condition, settlement in the river area, identifying pollution sources, and an on-going project in the river basin. Based on this information, and general environmental scientific knowledge, a conceptual model was developed. This conceptual modeling was passed through the four broad steps; each step involved several key activities that support developing an integrated model. Three correlated sub-models were developed first; finally, one integrated conceptual model was developed. Developing and application of an integrated model for Awetu river basin is very important to improve the current situation of the river. Achieving this needs an integrated work of all stakeholders and concerned bodies. Stakeholders recommended applying this model, whereas researchers recommended evaluation and modifying the model more. The model is also applicable for most rivers and surface water, particularly for waters with similar situations.

Heat domes increase vulnerability of native stingless bees by simultaneously weakening key survival traits

Heatwave events increase in frequency and duration, yet there is a strong gap in assessing their nonlethal effects on tropical insects, including beneficial social species. The stingless bees are a highly diverse group of pantropical pollinators that provide key ecosystem services. Here, we simultaneously analyzed for the first time the effect of sublethal heat stress (HS) during immature (pupal) development on adult morphology (size, shape, symmetry) and immune response of the three castes/sexes in stingless bee colonies: workers, unmated queens (gynes) and males, as well as its impact on the onset of foraging and lifespan in workers. Individuals experimentally heat stressed during development had smaller body size and reduced symmetry as adults compared with control, non-heat stressed (NHS) individuals, though the strength of the effects of HS also varied between castes and sexes. Notably, males were more prone to the effects of HS compared with workers, and less so gynes; HS reduced the immune response of males, though not that of workers or queens. Workers had significantly earlier onset of foraging and a shorter lifespan when exposed as immatures to HS. Under a worst-case scenario, knock-on negative impacts on individual survival caused by HS could compromise colony fitness. In the long-term, heatwaves may also have repercussions for the persistence of stingless bee species, the sustainability of key ancestral activities like meliponiculture and ecosystem services. Measures to ameliorate the effect of climatic warming are urgently needed to protect these pollinators, which represent an iconic world heritage.

Analyze the Similarities and Differences in EPC Mode Power Engineering Between China and Other Countries from the Perspective of Risk Avoidance in Enterprises

The subject of this paper is to explore the application of the EPC model in China and other countries electric power engineering projects. Engineering Procurement and Construction Mode refers to an engineering contracting mode under the deep intersection of Engineering, Procurement and Construction, where the project contractor integrates these key activities to improve the efficiency and quality of the project and control the cost and risk. However, due to the differences in culture, market and development history between the policies of China and other countries, the EPC model applicable to China's power engineering projects is not fully applicable to power engineering projects in international cooperation, which leads to the obstruction of Chinese power enterprises in participating in the construction of international cooperation. To delve deeper into the applicability and risk management of the EPC model. This study employs methods such as case study, literature review, and comparative analysis. Considering the current market regulatory requirements for risk management requirements of power engineering project strict, increasing attention, the power engineering project risk management system, although relatively mature, but EPC mode power project as a new way of cooperation, there is still a risk identification difficulty, risk evaluation error, and many other problems, combined with EPC mode project general contractor responsibility, high risk, so further project risk research is of great significance.

ВИКОРИСТАННЯ ЕЛЕКТРОННИХ РЕСУРСІВ НА РІЗНИХ ЕТАПАХ УРОКУ ІНФОРМАТИЧНОЇ ОСВІТНЬОЇ ГАЛУЗІ В ПОЧАТКОВИХ КЛАСАХ

The article analyses the peculiarities of conducting a competence-based lesson «I Explore the World» in the field of computer science. It is explained why the main type of computer science lesson in primary school is a combined lesson. The main stages of a combined lesson in the field of computer science and its structural components are characterized, described and presented graphically. The timing of each lesson block is scheduled, taking into account the recommendations of the Ministry of Education and Science and the requirements of the Sanitary Regulations. For each component, a selection of key learning activities is offered that can be used during this part of the lesson. The main didactic criteria that should be taken into account when choosing or developing electronic resources to support the teaching of computer science in primary school are systematized and described. The importance of the organizational component of the computer science lesson in primary school as necessary to ensure the safety of students in the computer classroom is determined. An electronic resource for teaching safety rules has been selected, which will help to better memorize the educational material. The necessity of using health-saving technologies is substantiated, their place in the overall structure of the lesson is determined. Specific tools and a selection of electronic resources for accompanying movements and gymnastics are described. The author explains the need to divide the study of a new topic into two stages: the research stage and the practical stage. The methodology for conducting the research stage with the use of time constraints is described, and free electronic resources for explaining the material of the new topic are selected. Free electronic resources, software environments and integrated educational and developmental software packages for primary school students, which are advisable to use at the stage of formation and improvement of practical skills, are considered. Electronic resources have been selected for use at the final stage of the lesson, which help to consolidate the learning material, develop critical thinking and provide feedback between participants in the educational process.

Business Development Strategy with SWOT Analysis Approach and Business Model Canvas at Wink Beauty Studio

This research aims to analyze business development strategies at Wink Beauty Studio using the SWOT Analysis and Business Model Canvas approaches. This approach aims to provide a comprehensive picture of the company's internal and external conditions and formulate the right strategy for business development. The research method used is descriptive qualitative, with data collection through in-depth interviews, observation, and documentation studies. The collected data were analyzed using a SWOT matrix to identify the strengths, weaknesses, opportunities, and threats faced by the company. The results of the SWOT analysis were then mapped into the nine elements of the Business Model Canvas, which include customer segments, value propositions, distribution channels, customer relationships, revenue streams, key resources, key activities, key partners, and cost structures. The research findings show that Wink Beauty Studio has several key strengths, such as superior service quality and customer loyalty, but also faces challenges, such as intense competition and limited resources. Development opportunities lie in the growing beauty trend and the potential of the digital market. By capitalizing on internal strengths and external opportunities, the company can develop more effective strategies to expand market reach and increase profitability. This research is expected to contribute to the management of Wink Beauty Studio in formulating the right strategic steps and become a reference for other beauty business actors in developing their businesses.

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets.

A subset of circulating human platelets stores Tissue Factor (TF) intracellularly, the key activator of the blood coagulation cascade and thrombus formation. Upon platelet activation, TF is exposed on the cell membrane, where it binds to FVII, ultimately leading to thrombin generation. Considering that (1) levels of TF-positive platelets increase in various clinical settings, contributing to the patient's prothrombotic phenotype, and (2) different drugs can modulate platelet-associated TF expression, a standardized method for assessing TF-positive platelets is valuable, as its evaluation has been controversial in the past. Here, we outline a protocol for measuring the percentage of TF-positive platelets using flow cytometry in whole blood and platelet-rich plasma (PRP)/washed platelets. This protocol aims to provide detailed instructions for quantifying the percentage of TF-positive platelets by assessing the protein (1) intracellularly in resting conditions, and (2) on the cell surface, in both resting and activated conditions. The first section provides essential information for correctly performing blood withdrawal to ensure that pre-analytical procedures do not affect the results. Next, the protocol focuses on sample preparation and labeling procedures for flow cytometry analysis. Detailed steps for cell stimulation, labeling, fixation, and permeabilization -- where necessary -- are outlined. Finally, instructions for flow cytometry settings to correctly identify the platelet population and analyze TF-positive events are described. Lastly, the method includes the procedure for preparing PRP if TF-positive platelets are to be measured in isolated platelets. Since only a subset of platelets contains TF, it is important to ensure that these platelets are not lost during the centrifugation steps required to obtain PRP.

Quality differences in sea buckthorn (Hippophaë rhamnoides L.) berries of major varieties in China based on key components and antioxidant activity

Sea buckthorn is rich in active ingredients, widespread, and has both medicinal and nutritional value. The present comparative study of wild and cultivated species remains insufficient, which is not conducive to their quality control. Therefore, this study aimed to compare the differences of 21 sea buckthorn samples in total phenolic content (TPC), total flavonoid content (TFC), phenolic components content, secondary metabolites, and antioxidant capacity and the fatty acid, to investigate the quality differences of different varieties. The TPC, TFC and antioxidant activity of wild varieties were higher than those of the cultivated. Multivariate statistical analysis revealed large differences in phenolic content, with higher levels of gallic acid and isorhamnetin-3-O-neohesperidin in the wild, whereas the cultivated were characterized by narcissin and kaempferol. These findings provided the scientific basis for the improvement of quality evaluation standards for different varieties and offered new insights for the further development of sea buckthorn resources.