Kelp Extract Research Articles

Extraction of Fucoxanthin from Marine Organisms and Its Therapeutic Effect on Cancer Cells

As China’s economy develops and its population grows, resources are becoming increasingly scarce. As a result, attention is shifting towards utilising marine resources, and the ability to process them is an increasingly important area of development.Within the traditional marine industry, kelp is primarily used for fucoidan extraction, with other substances such as kelp pigments often cast off as waste. Fucoxanthin constitutes the major carotenoid component found within kelp pigments. These pigments are believed to possess immunomodulatory, anti-inflammatory, and anti-tumour properties, and are of multifaceted benefit to human health by counteracting cellular oxidative damage induced by free radicals. Kelp served as the raw material in this study. Macroporous resin was utilized to extract Fucoxanthin from the kelp extract. Only an aqueous ethanol solution was employed for the extraction process. Subsequently, reduced-pressure column chromatography was conducted to quickly enrich the Fucoxanthin. Finally, the anti-tumour activity and free radical scavenging ability of the Fucoxanthin were analyzed. The results of the experiment revealed that Fucoxanthin can be effectively adsorbed by X-5 resin and its adsorption capability is robust. Additionally, using a 60% concentration of ethanol solution can promptly and effectively elute the absorbed Fucoxanthin from the X-5 resin. Moreover, after incubation for 48 hours at a concentration of 80 μg/mL of Fucoxanthin, the expression levels of p65, p52, and p50 genes significantly decreased in cancer cells. Fucoxanthin, a pigment found in kelp, exhibits potent antioxidant activity. The findings suggest that Fucoxanthin possesses the ability to effectively suppress the expression of cancer cells and scavenge free radicals.

Development of a Whey Protein Recovery Process Using Sugar Kelp (Saccharina latissima) Extracts.

Whey is the largest waste product of the cheese-making industry and the current methods of extracting the nutrients from it are costly and inefficient. This study assessed the feasibility of using crude polysaccharides to flocculate proteins from liquid whey waste. The flocculants used were a sugar kelp (Saccharina latissima) extract, as well as commercial seaweed polysaccharides, alginate and k-carrageenan, to recover proteins from the liquid whey waste. Physicochemical and functional parameters including protein content, protein recovery efficiency, mineral content, total phenolic content (TPC), antioxidant capacity, color, water- and oil-holding capacity, gelling capacity, foaming activity and stability, and emulsifying activity and stability were tested on the resulting flocculates. The yield of the dried flocculates by use of alginate, the sugar kelp polysaccharide extract (SKPE), and carrageenan were 1.66, 0.98, and 1.22 g/100 g of liquid whey with protein contents of 27.4%, 45.5%, and 37.5%, respectively. The protein recovery efficiency from the whey was 57.5%, 56.2%, and 57.9% using alginate, SKPE, and carrageenan, respectively. The alginate flocculate had the highest oil-holding capacity and foaming abilities while the carrageenan flocculate had the best gelling ability and the highest emulsifying activity and stability. TPC and antioxidant activity were highest in the SKPE flocculate. All three flocculates presented slightly different compositional and functional qualities, which could be used for a variety of products. This study showed that seaweed polysaccharides present a simple and effective way to extract protein from liquid whey waste while creating a functional and high-protein ingredient.

New biopolymers as viscosity-modifying admixtures to improve the rheological properties of cement-based materials

The performance of various new biopolymers as viscosity-modifying admixtures (VMAs), including tragacanth, almond, and locust bean gums and giant kelp extract, in addition to two commercial biopolymer-VMAs, welan and guar gums in cement-based materials was evaluated. The effect of the preparation/addition method, type, and dosage of the investigated biopolymers on rheology, hydration kinetics, stability, and compressive strength of various cement paste mixtures was evaluated. The pre-dispersion and pre-dispersion/pre-hydration techniques showed greater influence on the rheology of the investigated mixtures compared to dry addition. Moreover, the investigated biopolymers led to a shear-thinning response with higher viscoplastic properties than the reference. The structural build-up of the mixtures was mainly influenced by the type and dosage of the biopolymers, observing higher performances in mixtures containing guar gum, giant kelp extract, and welan gum. The use of the investigated biopolymers enhanced the stability of the paste mixtures, while negatively influenced the early-age strength development.

Spo0A-dependent antifungal activity of a plant growth promoting rhizobacteria Bacillus subtilis strain UD1022 against the dollar spot pathogen (Clarireedia jacksonii)

Dollar spot is an economically important foliar disease of turfgrass caused by Clarireedia spp. Increased use of chemical fungicides for disease management has possible negative effects on the environment and human health. The quest for eco-friendly alternatives to fungicides is driving the development of new biological strategies for managing dollar spot. In the present study, an analysis of antifungal activity of the plant growth promoting rhizobacteria (PGPR) Bacillus subtilis strain UD1022 (hereafter UD1022) and plant health products (PHPs) (Acibenzolar-S-methyl, kelp extract, fosetyl-Al, and trinexapac-ethyl) against Clarireedia jacksonii for control of the turfgrass fungal disease dollar spot was conducted. In vitro assays revealed that UD1022 has significant inhibitory effects against the mycelial growth of C. jacksonii. Of all the treatments, wild type UD1022 and a non-ribosomal peptide mutant (sfp¯) showed potential antagonism against C. jacksonii with inhibition rate of 17.2 % and 18.4%, respectively. Interestingly a Bacillus-derived broad antifungal compound (surfactin) and PHPs showed no direct antagonistic activity against dollar spot pathogen and were statistically like the untreated control. All the mutants of UD1022 except spo0A¯ showed varying but significant inhibition of the fungal pathogen suggesting that spo0A gene may be directly involved in the antagonism against C. jacksonii. Bacterial volatile compounds (BVCs) of UD1022 may not be involved in antagonism as no fungal inhibition was observed in the indirect assay using split petri plates. Filter sterilized cell free lysate (CFL) as well as heat treated CFL of UD1022 bacterial culture did not show any fungal inhibition indicating a primary role of live bacterial cells and not bacterial-derived or secreted compounds in fungal growth inhibition. The results indicate UD1022 to possess the potential to be extended for the biocontrol of dollar spot of turfgrass.

Measurements of elemental iodine in soy sauces in Taiwan using a modified microplate method.

Soy sauce is widely used in a variety of Asian dishes to enhance flavor. Soybean and most soybean products, including soy sauces, are listed as prohibited foods in a low iodine diet. However, the iodine content in soy sauces is largely unknown. The aim of this study was to determine the iodine content in domestic soy sauces in Taiwan. Twenty-five different kinds of soy sauces were diluted with distilled water and with a dilution factor of fifty or above. Iodine concentrations of the diluted samples were measured colourimetrically based on the Sandell-Kolthoff reaction by a modified microplate method. All the measurements were repeated twelve times on three different days for determination of mean and standard deviation (SD), and coefficients of variance (CV). Serial dilution and recovery tests were also performed for validation. The results were confirmed by an inductively coupled plasma mass spectrometry (ICP-MS) method. Among the twenty-five surveyed soy sauces, most of them (n=22) were iodine-free (<16 ug/L, and thus un-detectable). The iodine concentrations (mean ± SD) of the three iodine-containing soy sauces were 2.7 ± 0.1, 5.1 ± 0.2, and 10.8 ± 0.6 mg/L, respectively. The inter-assay, intra-assay and total CVs were all <5.3% for the modified microplate method. The results obtained by ICP-MS were consistent with those of the modified microplate method. The recovery rates in the serial dilution test and recovery test ranged from 94.7% to 118.6%. Two of the three iodine-containing soy sauces were supplemented with kelp extract, while the other one without kelp extract had the highest amount of salt among the three iodine-containing soy sauces. Therefore, we postulate that iodized salt instead of kelp extract is the source of higher iodine content in that sauce. The results suggest that most soy sauces are iodine-free and may be allowed during low iodine diets.

Fucoxanthin inhibits the proliferation and stem cell formation of oral squamous cell carcinoma (OSCC) cells by regulating JAK/STAT3 pathway

Purpose: To investigate the effect and regulatory mechanism of fucoxanthin (FX) on oral squamous cell carcinoma (OSCC).&#x0D; Methods: Human OSCC SCC9 and Cal27 cells were treated with different concentrations of FX (7.5, 15, and 30 μM) to determine cell viability, number of colonies, and apoptosis rate using methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay, crystal violet staining, and flow cytometry, respectively. In addition, the number of spheres of OSCC cells was determined by a cell sphere-forming assay. The stem cells and levels of pathway-related proteins were evaluated by western blotting.&#x0D; Results: Fucoxanthin decreased SCC9 and Cal27 cell viability and the number of colonies, but increased apoptosis in a dose-dependent manner. The expression levels of SOX2 and POU5F1 were down-regulated, while the number of spheres was reduced in SCC9 and Cal27 cells treated with 7.5 or 15 μM of FX (p &lt; 0.05). Moreover, FX attenuated p-JAK/JAK and p-STAT3/STAT3 expression levels in a dose-dependent manner (p &lt; 0.05).&#x0D; Conclusion: Fucoxanthin accelerates apoptosis and inhibits cell mobility and OSCC stem cell formation by suppressing JAK/STAT3 pathway in OSCC, thus providing an experimental basis for research on the anti-tumor effect of kelp extract and the development of new drugs from marine plants.

Stabilization Activity of Kelp Extract in Ethylene–Propylene Rubber as Safe Packaging Material

This paper presents the stabilization effects of the solid extract of kelp (Ascophyllum nodosum) on an engineering elastomer, ethylene-propylene copolymer (EPR), which may be used as packaging material. Progressive increase in additive loadings (0.5, 1, and 2 phr) increases the oxidation induction time for thermally aged rubber at 190 °C from 10 min to 30 min for pristine material and modified polymer by adding 2 phr protection powder. When the studied polymer is γ-irradiated at 50 and 100 kGy, the onset oxidation temperatures increase as a result of blocking the oxidation reactivity of free radicals. The stabilization effect occurs through the activity of alginic acid, which is one of the main active components associated with alginates. The accelerated degradation caused by γ-exposure advances more slowly when the kelp extract is present. The OOT value for the oxidation of EPR samples increases from 130 °C to 165 °C after the γ-irradiation of pristine and modified (2 phr of kelp powder) EPR, respectively. The altered oxidation state of EPR samples by the action of γ-rays in saline serum is faster in neat polymer than in stabilized material. When the probes are placed in physiological serum and irradiated at 25 kGy, the OOT value for neat EPR (145 °C) is much lower than the homologous value for the polymer samples protected by kelp extract (153 °C for the concentration of 0.5 phr, 166 °C for the concentration of 1 phr, and 185 °C for the concentration of 2 phr).

A Natural Bio-Stimulant Consisting of a Mixture of Fish Protein Hydrolysates and Kelp Extract Enhances the Physiological, Biochemical and Growth Responses of Spinach under Different Water Levels.

Spinach (Spinacia oleracea L.) is a highly nutritious, desirable green leafy vegetable, which is less tolerant to drought. This study was conducted to establish the impact of a natural bio-stimulant consisting of a mixture of fish protein hydrolysates and kelp extract (trade name, Xcell Boost) on the physiological and biochemical responses as well as vegetative growth of spinach (Spinacia oleracea L.) under different water levels (100% (full irrigation), 50% (mild drought stress) and 30% (severe drought stress) water holding capacity). Bio-stimulant application at any strength (single, BX1 or double, BX2) had no effect on the photochemical reactions. The application of bio-stimulant at double strength concentration (BX2) increased the chlorophyll and carotenoid contents, as well as the activities of antioxidative enzymes, ascorbate peroxidase (APX) and guaiacol peroxidase (GPX), under drought stress. Application at single strength (BX1) increased the normalised difference vegetation index (NDVI), stomatal conductance, accumulation of osmoprotectants (proline and total soluble sugars) and reduced electrolyte leakage under drought stress. Furthermore, bio-stimulant applications at either concentration induced remarkable increases in plant height, leaf area, stem dry weight, root length and root moisture. Under BX2, APX and stomatal conductance positively correlated with stem dry weight, while root length positively correlated with total chlorophyll content. These results show that Xcell Boost is a highly advantageous bio-stimulant for increasing the tolerance of spinach to drought stress, which can most likely benefit other crops grown in semi-arid and arid areas.

Effect of biostimulant and biofertilizers on soil bio-chemical properties and plant growth of apple (Malus x domestica Borkh.) nursery

Aim: To assess the effect of biostimulant and biofertilizers on soil properties and plant growth of apple nursery under eco-friendly production system. Methodology: The experiment was laid out in a randomized block design comprising 18 treatments of biostimulant, biofertilizers and their combinations. Each treatment was replicated thrice. Nursery beds of apple seedling rootstocks were prepared and Arbuscular mycorrhizal fungi, PGPR (Bacillus sp.) were applied by soil drenching method and commercial formulations of granular bio-stimulant containing humic acid (39%) + kelp extract (25%) + amino acids (9.0%) + ascorbic acid + thiamine + inositol + tocopherol (27%) were applied in two split doses by broadcasting in root zone. The effects of these various formulations were investigated during the course of studies. Results: Linear growth, radial growth of scion (6.66 mm), number of leaves per plant, leaf area, success rate, saleable plants, total root length, fresh weight of shoots, dry weight of shoots, fresh weight of roots, dry weight of roots and total plant biomass of apple nursery plants were significantly increased with the combined application of biostimulant (200 g m-2 nursery bed) + AM fungi (20 g per plant) + PGPR (50 ml per plant). This treatment also significantly improved the status of available soil N, P, K and population of fungi, bacteria as well as actinomycetes in the rhizosphere. Interpretation: Application of biostimulant (200 g m-2 nursery bed) + AM fungi (20 g per plant) + PGPR (50 ml per plant) proved to be the most effective combination of eco-friendly inputs for the production of good quality apple nursery plants with well developed shoot and root system and improved the soil bio-chemical properties.

The study of the intensification of technological parameters of the sausage production process

One of the sources of sodium are meat products. Increased consumption of meat products and sodium intake leads to serious health problems. The task of reducing the dosage of sodium chloride in minced meat needs to be addressed. The partial replacement of table salt with sea salt will reduce the sodium concentration in products to 20%. It is established that this modification increases the moisture-binding properties of minced meat and lowers the dosage of salt in the mass of raw meat, which will reduce the level of harm to the body due to excessive consumption of sausages. It is proposed to introduce a bacterial preparation based on the strain Staphylococcus carnosus, which will reduce the amount of sodium nitrite in the finished products. Technology has been developed to regulate the composition of microelements in meat products by enriching them with a kelp extract. It was found that changes in the composition of minced meat can adversely affect the taste and physicochemical properties of the product, which is confirmed by expert studies. As a result of laboratory studies, it was found that a partial change of salt in the sea helps to improve the stability and physicochemical quality of minced meat (active acidity, water activity, moisture retention, and shear stress). According to the research results, the recipe of sausages recommended for implementation at the enterprises of the meat processing industry of Ukraine has been developed.

Исследование возможности применения гранулированного сахаросодержащего продукта с функциональными добавками при производстве желейных начинок

Introduction. Iodine is one of the most important elements for maintaining human health and cognitive skills. Contemporary food industry is looking for new functional foods fortified with macro- and micronutrients. Confectionery products occupy a fairly large segment of human diet. The present research objective was to substitute traditional white sugar with an experimental granulated sugar-containing product that contained maltodextrin, cane molasses, and Japanese kelp extract. This substitute could reduce the calorie content of jelly and increase its functional properties while maintaining its sensory profile, e.g. consistency, increasing its shelf-life, and simplifying the tec hnological process.&#x0D; Study objects and methods. Granulated sugar-containing product is a new component for the confectionery industry, but its effect on technological processes is unpredictable. The research featured an experimental granulated sugar-containing product with maltodextrin, cane molasses, and Japanese kelp. The growth rate of granules and their structure were studied by scanning microscopy and radiography, the granulometric composition and solubility – by determining the relative dissolution rate, the effect of the granulated sugar-containing product on the sensory and physicochemical parameters of jelly – by refractometry and titrometry.&#x0D; Results and discussion. The experimental granulated sugar-containing product contained sucrose both in crystalline and amorphous state, which affected the distribution of nutrients within the product. The growth rate of granules affected the resulting structure of the product and its solubility, which decreased by 2.5 times as the size of the granules grew. The dissolution rate decreased compared to crystalline sugar. As a result, the technological process of jelly production had to be adjusted. The developed jelly had a lower energy value and better nutritional qualities. The high content of vitamins and macro- and microelements makes it a functional product. The experimental jelly received 22.3 points for sensory profile. Its hysico-chemical parameters (soluble solids &lt; 68%, acidity &gt; 2.2 8%) met the standard requirements for this type of product.&#x0D; Conclusion. The new granulated sugar-containing product simplified the technological process and improved the sanitary, hygienic, and working conditions.

Photochemical efficiency, biomass and chlorophyll of phyális under salinity and biostimulant

Physalis peruviana L. has great exploitation potential especially for small producers in the Brazilian Northeast. However, production in semiarid regions may be limited by high salt content in soils and water sources used for irrigation. Thus, this study evaluated the application of Ascophyllum nodosum seaweed extract as saline stress attenuator in P. peruviana L. The randomized block design was used, in an incomplete 5 × 5 factorial scheme, with five of electrical conductivities of the irrigation water (0.50, 1.23, 3.00, 4.77 and 5.50 dS m-1) and five increasing concentrations of kelp extract (0.00, 1.45, 5.00, 8.55 and 10.00 mL L-1), with nine combinations generated by the Central Box matrix, with four replicates of four plants to evaluate the effect on phytomass accumulation, chlorophyll fluorescence and pigment content. Results showed, salinity significantly reduced biomass production and chlorophyll fluorescence and pigment indices. However, when plants were treated with 9.9 mL L-1 seaweed extract they increased biomass production and seedling quality. On the other hand, up to 4.1 mL L-1, the biostimulant increased dark-adapted fluorescence indices which indicate it reduces damages to the photosynthetic apparatus caused by salt-stress and thus improving photosynthetic activity and photoassimilates production. Thus the application of A. nodosum seaweed extract attenuates the deleterious effect of salt stress in photosynthesis and thus in biomass production of P. peruviana plants.

Chemotaxis Assay for Marsupenaeus japonicas Hemocytes and Application for the Development of an Oral Immunostimulant Against White Spot Syndrome Virus.

The chemotactic activity of vertebrate leukocytes is an important host-defense mechanism. However, chemotaxis of invertebrate immune cells, particularly those of shrimp species, is incompletely understood and critically understudied. In this study, we aimed to optimize the conditions for a Boyden chamber chemotaxis assay using hemocytes (granulocytes) from cultured kuruma shrimp, Marsupenaeus japonicas (Mj) and the optimal conditions were: 5 μm-pore-size Polyvinylpyrrolidone membrane; culture buffer at pH 7.0; and chemotactic factor N-formyl-methionyl-leucyl-phenylalanine (fMLP) 10–8 mol/L; 4 h incubation time. We then applied the chemotaxis assay to develop an oral immunostimulant against white spot syndrome virus (WSSV), which results in high mortality rates in several shrimp species worldwide. We focused on the kelp Laminaria japonica, as this species contains immunostimulative molecules such as β-glucan. We prepared Heat Extracts (HE) and Crude Laminarans (CL) from kelp using hot water and hydrochloric acid extraction methods, respectively. HE and CL ware mixed with normal shrimp feed. Kelp extracts were orally administered for 7 days, and hematocyte chemotaxis toward fMLP was compared. No difference was detected between control and kelp extracts on day 3, but HE stimulated chemotaxis 2-fold and CL stimulated chemotaxis 3-fold relative to control on day 7 after initiating administration. Kelp extract administration protected against WSSV exposure. Finally, we identified that Kelp extracts stimulated hematocyte superoxide production on days 3 and 7, and increased hematocyte phagocytosis and phenol oxidase activity on day 7 after administration. We concluded that the chemotaxis assay is informative in assessment of shrimp hemocyte immunological activity, and is applicable to the development of immunostimulants against shrimp infectious diseases.

IMPACT OF PREPARED DIET ON THE GROWTH AND DEVELOPMENT OF PUPPIES

The authors see energy components extremely relevant for animals and their health. Due to this fact, diets with energy components that satisfy pets with nutrients at different stages of growth is significant issue in veterinary nutrition. This could be achieved when applying diets that contain balanced necessary components. The research aimed at exploring the impact caused by ready diets on the growth and development of dogs that differ in age and breed is fragmentary one. The authors make a case about necessity for detailed investigation the growth parameters of animals when feeding them with this kind of diets in the low temperatures of Western Siberia. The impact of the finished ration on puppy growth and development was investigated in one of the s animal shelters in Novosibirsk in 2016. The main food used in the experiment for feeding puppies was YUMMI PREMIUM; the feed contains proteins - 26%, fats - 10, fiber - 7, ashes - 7, moisture - no more than 10, calcium – 1 and phosphorus - 0,8%. The authors observed that 50% are meat ingredients (beef, chicken, by-products), 35 are cereals (wheat, buckwheat), vegetable oils, dried milk and 5% are vegetables, chicken fat, mannanoligosaccharides (MOS), Shidiger yucca extract and sea kelp extract. The following food additives are included in the feed: Vitamin A (17000 IU/ kg), vitamin D3 (1700 IU/kg), vitamin E (200 mg/kg), B1 (15 mg/kg), B2 (20 mg/kg), B6, B12 (100 mg/kg), K3, niacin (85 mg/kg), pantothenic acid (50 mg/kg), biotin (950 µg/kg), iron, zinc, manganese, copper (20 mg/kg), iodine (2 mg/kg) and selenium (0.25 mg/kg). The food of the experiment was eaten by puppies with great pleasure. This experiment has shown that YUMMI PREMIUM for puppies increase body weight and average daily growth rates, as well as improve the main parameters of dogs development.

Evaluation of Products to Alleviate Irrigation Salinity Stress on Bermudagrass Turf

Core Ideas Availability of water in arid zone may prevent the use of leaching; therefore, alternative methods need to be identified to manage salinity. Identify which commercially available products that are supposed to ameliorate soil chemical and physical characteristic have a real impact on soil chemistry. Test if soil salinity is affected not only by irrigation practices, but also other environmental factors, such as evapotranspiration. Increasing salinity issues caused by insufficient precipitation, drought, and increasing use of alternative nonpotable sources of irrigation water are a major concern for turf management in the southwestern United States. Modification of soil physicochemical properties that result from salinity is one means of alleviating plant salinity stress. A field study was conducted in 2013 and 2014 at the University of California, Riverside turfgrass research facility in Riverside, CA. Six commercial programs were tested for their ability to alleviate salinity stress on ‘Tifway II’ hybrid bermudagrass [Cynodon dactylon (L.) Pers. × C. transvaalensis Burtt‐Davy] irrigated with saline water (electrical conductivity = EC ≈ 4.4 dS m−1). These included: CrossOver (calcium silicate, magnesium silicate, and monosilicic acid); Revert (polyalkylene glycol); SUNBurst Cal Plus 8% (calcium carbonate and calcium nitrate) applied at 0.23 or 0.47 mL m−2; a combination of DeSal (carboxylic acid), Stress Rx (potassium nitrate, potassium acetate, and calcium nitrate) and XP Micro (urea, magnesium nitrate, manganese nitrate, manganese sulfate, ferrous sulfate, iron EDTA, kelp extract); and Turfcare Natural Plant Nutrient (Meliaceae spp. extracts). Plots were evaluated every 2 wk for visual turf quality, and Digital Image Analysis. Leachate was also collected and analyzed for EC on the same day. Soil samples were collected and analyzed for salinity at the end of each growing season. DeSal + Stress RX + XP Micro was the only treatment that resulted in acceptable visual quality (6.4) and decreased sodium adsorption ratio (SAR) and Na content in the rootzone by the end of the study.

Prediction of skin anti-aging clinical benefits of an association of ingredients from marine and maritime origins: Ex vivo evaluation using a label-free quantitative proteomic and customized data processing approach.

The application of ingredients from marine and maritime origins is increasingly common in skin care products, driven by consumer expectations for natural ingredients. However, these ingredients are typically studied for a few isolated invitro activities. The purpose of this study was to carry out a comprehensive evaluation of the activity on the skin of an association of ingredients from marine and maritime origins using label-free quantitative proteomic analysis, in order to predict the clinical benefits if used in a skin care product. An aqueous gel containing 6.1% of ingredients from marine and maritime origins (amino acid-enriched giant kelp extract, trace element-enriched seawater, dedifferentiated sea fennel cells) was topically applied on human skin explants. The skin explants' proteome was analyzed in a label-free manner by high-performance liquid nano-chromatography coupled with tandem mass spectrometry. A specific data processing pipeline (CORAVALID) providing an objective and comprehensive interpretation of the statistically relevant biological activities processed the results. Compared to untreated skin explants, 64 proteins were significantly regulated by the gel treatment (q-value≤0.05). Computer data processing revealed an activity of the ingredients on the epidermis and the dermis. These significantly regulated proteins are involved in gene expression, cell survival and metabolism, inflammatory processes, dermal extracellular matrix synthesis, melanogenesis and keratinocyte proliferation, migration, and differentiation. These results suggest that the tested ingredients could help to preserve a healthy epidermis and dermis, and possibly to prevent the visible signs of skin aging.

Properties of Kimchi Fermented with GABA-Producing Lactic Acid Bacteria as a Starter.

Kimchi (a traditional Korean fermented vegetable) was prepared with a starter, Lactobacillus zymae GU240 producing γ-aminobutyric acid (GABA), and one precursor of GABA (glutamic acid, glutamic acid monosodium salt (MSG), or kelp extract). L. zymae GU240, an isolate from kimchi, can grow at 7% NaCl and low temperature. Five different kimchi samples were fermented for 20 weeks at -1°C. Kimchi with starter alone could not produce GABA. The GABA content was highest in kimchi with co-inoculation of the starter and MSG (1% (w/w)). Kimchi co-inoculated with the starter and kelp extract powder (3% (w/w)) had the second highest GABA content. Addition of glutamic acid powder (1% (w/w)) caused a reduction in the pH level of kimchi and growth inhibition of lactic acid bacteria and yeasts. Kimchi samples with MSG or kelp extract showed improvement of sensory evaluation scores. The results demonstrate the possibility to produce kimchi with improved functionality and taste by using L. zymae GU240 as a starter along with a suitable precursor such as MSG or kelp extract.

Experimental Study of Different Effective Part of the Solvent Extraction of Kelp

In this study, seaweed kelp, respectively, with petroleum ether, chloroform, ethyl acetate, n-butyl alcohol, distilled water kelp extract effective parts, and its contents were measured. The results show that: effective parts kelp has some antioxidant properties, its strength depends on the extraction solvent, with different organic solvents for extraction kelp, each extract antioxidant activity order: ethyl acetate extract&gt; chloroform extract&gt; n-butanol extract&gt; aqueous layer extract. Each component seaweed extract contains flavonoids and polyphenols composition and content of different types, but most of ethyl acetate containing the sum of both.

Kelp extract (Ascophyllumnodosum) can improve health and drought tolerance of green roof plants

Kelp extract (Ascophyllumnodosum) can improve health and drought tolerance of green roof plants

Corrosion protection of reinforcing steel with kelp extract as corrosion inhibitor

This study aims to evaluate the effectiveness of kelp extract as a corrosion inhibitor to resist the steel corrosion in mortars. The solvent extraction methods with several technologies were applied to prepare the kelp extracts, and their inhibiting efficacies were compared in the saturated calcium hydroxide solution. Besides, the extract with the best inhibition effect in the solution was applied in mortars. Several electrochemical techniques, compressive strength and flexural strength tests and visual inspection of steel surface were performed. Results indicate that all the extracts have an ability of inhibiting the steel corrosion in the solution. In principle, they are classified as a mixed inhibitor. The extract prepared by using 0·1 mol L−1 NaOH as a solvent exhibits a good inhibition effect in mortars. However, it decreases the compressive strength and flexural strength of mortar. Accordingly, the kelp extract is suggested to be applied cautiously as an inhibitor in the reinforced concrete.