Kanchi Kamakoti CHILDS Trust Hospital Research Articles

Changing Landscape of Antimicrobial Resistance in Neonatal Sepsis: An in silico Analyses of Multidrug Resistance in Klebsiella pneumoniae.

Neonatal sepsis poses a critical healthcare concern, as multidrug-resistant Klebsiella pneumoniae ( K. pneumoniae ) infections are on the rise. Understanding the antimicrobial susceptibility patterns and underlying resistance mechanism is crucial for effective treatment. This study aimed to comprehensively investigate the antimicrobial susceptibility patterns of K. pneumoniae strains responsible for neonatal sepsis using in silico tools. We sought to identify trends and explore reasons for varying resistance levels, particularly for β-lactams and fluoroquinolone. K. pneumoniae isolated from neonates at Kanchi Kamakoti CHILDS Trust Hospital (2017-2020) were analyzed for antimicrobial resistance. Elevated resistance to β-lactam and fluoroquinolone antibiotics was further investigated through molecular docking and interaction analysis. β-lactam affinity with penicillin-binding proteins and β-lactamases was examined. Mutations in ParC and GyrA responsible for quinolone resistance were introduced to investigate ciprofloxacin interactions. Of 111 K. pneumoniae blood sepsis isolates in neonates, high resistance was detected to β-lactams such as cefixime (85.91%, n = 71), ceftriaxone (84.9%, n = 106), cefotaxime (84.9%, n = 82) and fluoroquinolone (ciprofloxacin- 79.44%, n = 107). Molecular docking revealed low β-lactam binding toward penicillin-binding proteins and higher affinities for β-lactamases, attributing to the reduced β-lactam efficiency. Additionally, ciprofloxacin showed decreased affinity toward mutant ParC and GyrA in comparison to their corresponding wild-type proteins. Our study elucidates altered resistance profiles in neonatal sepsis caused by K. pneumoniae , highlighting mechanisms of β-lactam and fluoroquinolone resistance. It underscores the urgent need for the development of sustainable therapeutic alternatives to address the rising antimicrobial resistance in neonatal sepsis.

Correlation of SARS-CoV-2 Serology and Clinical Phenotype Amongst Hospitalised Children in a Tertiary Children's Hospital in India.

IntroductionChildren usually present with minimal or no symptoms of COVID-19 infection. Antibody responses to SARS-CoV-2 in children from low- and middle-income countries (LMIC) have not been well described. We describe the prevalence of anti-SARS-CoV-2 antibodies and clinical phenotype of seropositive children admitted to a tertiary children’s hospital in South India.MethodsTo determine the seropositivity and describe the clinical characteristics of COVID-19 infection amongst hospitalised children, we performed a prospective clinical data collection and blood sampling of children admitted to Kanchi Kamakoti CHILDS Trust Hospital, Chennai, India over 4 months of the COVID-19 pandemic. In seropositive children, we compared antibody titres between children with and without PIMS-TS.ResultsOf 463 children, 91 (19.6%) were seropositive. The median (range) age of seropositive children was 5 years (1 month–17 years). Clinical presentation was consistent with Paediatric inflammatory multisystem syndrome associated or related with SARS-CoV-2 infection (PIMS-TS) in 48% (44/91) of seropositive children. The median (range) antibody titre was 54.8 (11.1–170.9) AU/ml among all seropositive children. The median antibody titre among the children with PIMS-TS (60.3 AU/mL) was significantly (p = 0.01) higher when compared to the children without PIMS-TS (54.8 AU/mL).ConclusionWe describe the antibody responses to SARS-CoV-2 amongst hospitalised children in a LMIC tertiary children’s hospital. Almost half of the seropositive children had PIMS-TS. Antibody levels may be helpful in the diagnosis and disease stratification of PIMS-TS.LAY SUMMARYChildren usually present with minimal or no symptoms of COVID-19 infection. However, Multisystem Inflammatory Syndrome in Children (MIS-C) or Paediatric inflammatory multisystem syndrome associated or related with SARS-CoV-2 infection (PIMS-TS) has emerged as a distinctive paediatric illness related to SARS-CoV-2. Recently, antibody testing for SARS-CoV-2 is being used increasingly as a diagnostic test for PIMS-TS. However, data on the antibody responses to SARS-CoV-2 in children are sparse. We, therefore, attempted to identify the seropositivity and describe the clinical spectrum of COVID-19 infection amongst infants and children getting hospitalised in a children’s hospital in south India. Nearly one-fifth of the hospitalised children tested serology positive over 4 months. Antibody levels in children with PIMS-TS were significantly higher in comparison to the other two groups (acute COVID-19 infection and children without PIMS-TS). Results from our study suggest that all children are at risk of COVID-19 infection though they may present with mild illness or no symptoms. We also observed that antibody testing may have a possible role in diagnosis of PIMS-TS.

From the Case Records of Kanchi Kamakoti CHILDS Trust Hospital: Fever with Vesiculobullous Lesions

From the Case Records of Kanchi Kamakoti CHILDS Trust Hospital: Fever with Vesiculobullous Lesions

From the Case Records of Kanchi Kamakoti CHILDS Trust Hospital: An Adolescent with Recurrent Meningitis

From the Case Records of Kanchi Kamakoti CHILDS Trust Hospital: An Adolescent with Recurrent Meningitis

An Adolescent with Jaundice and Respiratory Distress from the Case Records of Kanchi Kamakoti CHILDS Trust Hospital

An Adolescent with Jaundice and Respiratory Distress from the Case Records of Kanchi Kamakoti CHILDS Trust Hospital

From the Case Records of Kanchi Kamakoti CHILDS Trust Hospital: An Infant with Post-varicella Angiopathy (Stroke)

From the Case Records of Kanchi Kamakoti CHILDS Trust Hospital: An Infant with Post-varicella Angiopathy (Stroke)

From the Case Records of Kanchi Kamakoti CHILDS Trust Hospital: An Unusual Infection Presenting as Pyrexia of Unknown Origin

From the Case Records of Kanchi Kamakoti CHILDS Trust Hospital: An Unusual Infection Presenting as Pyrexia of Unknown Origin

Sensitivity pattern and trend of multi drug resistance in Salmonella typhi occurring in South India

In a prospective cross sectional study conducted at Kanchi Kamakoti Childs Trust hospital, a major private tertiary care hospital for children in Chennai, India during the period October 2013 to September 2014. 105 cases were diagnosed as typhoid with their blood culture yielding the growth of Salmonella typhi. Those children were followed with drug sensitivity pattern and the trend of sensitivity was compared.

A Hospital-Based Epidemiologic Study on Acute Pediatric Poisonings in Chennai, India

Background: Toxic exposures in childhood are major health concern. In this hospital-based study, we sought to investigate socio-epidemiological factors contributing to acute pediatric poisoning in Chennai, Tamil Nadu, India. Methods: This prospective cross-sectional study was conducted at the Kanchi Kamakoti CHILDS Trust Hospital (KKCTH), a tertiary care hospital for children in Chennai. Children and adolescents less than 18 years of age with diagnosis of acute poisoning during June 2014 to January 2015 were included in the study. Results: During the study period, 10500 children were admitted to emergency department of the hospital; among which, 34 children presented with diagnosis of acute poisoning (0.32% of admissions). Eighteen patients (52.9%) were boys. The greatest proportion of patients (52.9%) aged 1 to 3 years. Regarding the intention of poisoning, 27 cases (79.4%) occurred following unintentional ingestion by children, 5 cases (14.7%) following inadvertent administration of medication(s) by a caregiver and 2 cases (5.9%) following inadvertent administration by a sibling. Children had relatively equal chance of being poisoned with medications (n = 18, 52.9%) and common household agents (n = 16, 47.1%). The most common medicines responsible for the poisonings were neuropsychiatric medicines (n = 6, 17.6%). None of the medications responsible for poisoning had childproof containers. On admission, only 14 children (41.2%) were symptomatic. Fifteen patients (44.1%) required admission to hospital wards and 4 patients (11.8%) required intensive care. The remaining patients only needed close observation for a few hours. All children made complete recovery and there was no mortality. Conclusion:Children especially toddlers of either gender are vulnerable to unintentional exposures and need constant supervision by an adult. Educating caregivers about the fatalities associated with unprotected storage of medications, and dangers of placing hazardous chemicals in the reach of children will reduce a great number of poisoning in children.

Correlation of Bone Mineral Parameters with Anthropometric Measurements and the Effect of Glucocorticoids on Bone Mineral Parameters in Congenital Adrenal Hyperplasia.

To correlate the bone mineral parameters [bone mineral content (BMC) and bone mineral density (BMD)] using Dual energy X ray Absorptiometry (DXA) scan with anthropometric measurements and to study the effect of glucocorticoid therapy on BMC/BMD in children with Congenital adrenal hyperplasia (CAH). A cross-sectional study was carried out in the Pediatric Endocrinology unit from January 2012 through March 2013 at Kanchi Kamakoti CHILDS Trust hospital, Chennai. Thirteen CAH children aged 0-132 mo with classic salt wasting due to 21 hydroxylase deficiency were included in the study. All children were treated with T.hydrocortisone @10-15 mg/m(2)/d twice daily and T. fludrocortisone 50 μg once daily orally at the time of enrollment into the study. The duration of glucocorticoid (hydrocortisone) treatment from the date of diagnosis till the time of enrollment into the study was noted and categorized as children receiving < 5 and > 5 y of glucocorticoid therapy. None received Vitamin D/calcium supplementation at the time of enrollment. BMC and areal BMD for the lumbar spine and total body less head (TBLH) were measured with Lunar DXA machine. The mean height, weight and BMI of children were 87.3 ± 33 cm, 13.49 ± 11.2 kg and 14 ± 4.07 kg/m(2) respectively. TBLH BMC was 369.14 ± 312.18 g and TBLH BMD was 0.63 ± 0.11 g/cm(2). There was a significant correlation between height and total body less head BMC/BMD in the index series [P < 0.05, significant]. The TBLH and spine BMD were also assessed with regards to the duration of glucocorticoid therapy and it has been observed that TBLH and spine BMD decreased with increased duration of steroid therapy (p < 0.05, significant). In the absence of normative data or z scores, BMC/BMD correlates well with height for age. Children who received more than 5 y of glucocorticoid treatment had lower TBLH and spine BMD scores and hence, calcium and Vitamin D supplementation should be considered.

Impact of cancer support groups on childhood cancer treatment and abandonment in a private pediatric oncology centre.

Aims:To analyze the impact of two cancer support groups in the treatment and abandonment of childhood cancer.Materials and Methods:This is a retrospective review of children with cancer funded and non-funded who were treated at Kanchi Kamakoti CHILDS Trust Hospital from 2010 to 2013. A total of 100 patients were funded, 57 by Ray of Light Foundation and 43 by Pediatric Lymphoma Project and 70 non-funded.Results:The total current survival of 80%, including those who have completed treatment and those currently undergoing treatment, is comparable in both the groups. Abandonment of treatment after initiating therapy was not seen in the financially supported group whereas abandonment of treatment after initiation was seen in one child in the non-funded group.Conclusions:Besides intensive treatment with good supportive care, financial support also has an important impact on compliance and abandonment in all socioeconomic strata of society. Financial support from private cancer support groups also has its impact beyond the patient and family, in reducing the burden on government institutions by non-governmental funding in private sector. Improvement in the delivery of pediatric oncology care in developing countries could be done by financial support from the private sector.

Management of acute complications of pediatric burns - our experience of 7 years

Material and Methods: This is a retrospective review of children with burns admitted to the Kanchi Kamakoti Childs Trust Hospital, a 220-bed tertiary-care children's hospital in Chennai, India. Results: Four hundred and twenty-nine children with burns were admitted and treated between 2007 and 2014. Among these, 40 patients with over 20% deep and deep partial thickness burns were transferred to the Pediatric Intensive Care Unit (PICU) for various acute complications. In the presence of invasive sepsis or complication at the time of admission or later, the children are transferred to the PICU. Of the 40 cases transferred, 13 died and 27 survived. Details of the management of these complications and the causes of death were analyzed. It was realized that the survival of 27 burnt children was possible due to the combined team approach of treatment by the burn and plastic surgeons and the intensivists of the PICU. Conclusion: Continued approach is necessary for better survival in pediatric burns.

Serum zinc levels are low in children with simple febrile seizures compared with those in children with epileptic seizures and controls

To investigate serum zinc levels in children with simple febrile and epileptic seizures and compare them with controls. A prospective, case-control study was conducted between July 2009 and September 2010 in Kanchi Kamakoti CHILDS Trust Hospital, Chennai. Sixty-seven children (22 with epileptic seizures, 23 with simple febrile seizures and 22 controls) were enrolled. Serum zinc level was estimated using atomic absorption spectrometry. Mean (SD) serum zinc level in children with epileptic seizures, simple febrile seizures and controls were 969·8 (177·3), 638·1 (206·8) and 939·9 (258·4) ug/L, respectively (p<0·05). Serum zinc levels are lower in children with febrile seizures than in those with epileptic seizures and normal children. Zinc supplementation of children at risk of febrile seizures requires further study.

Letters to the Editor

Letters to the Editor

Kawasaki disease in south India: a prospective, case–control study

To describe the clinical and laboratory profile of Kawasaki disease in south India and compare patients with normal and abnormal cardiac status. This was a nested case-control study. All patients admitted to Kanchi Kamakoti CHILDS Trust Hospital, Chennai with Kawasaki disease between January 2002 and March 2006 were included. They were stratified into two groups: cases, who had abnormal echocardiography (ECHO), and controls, who had normal ECHO. All clinical and laboratory investigations, results of echocardiography and management were recorded and follow-up was organised. Of 56 patients, 36 (64.3%) were boys and 58.9% were under 3 years of age. Only 17.9% had an atypical presentation. Oral cavity changes and cervical lymphadenopathy were the most common clinical parameters and were detected in 85.7%. Thrombocytosis and elevated CRP on admission were seen in 41% and 100%, respectively. Echocardiography was abnormal in 27 (48.2%). Apart from raised CRP, which was higher in those with cardiac abnormality, the clinical and laboratory profiles were similar in the two groups. Kawasaki disease is not uncommon in south India. The clinical and laboratory profiles were similar between those with and without cardiac echocardiographic abnormality.

Interstitial lung disease in infancy

To describe the clinical profile of interstitial lung disease in infancy. A retrospective analysis of cases diagnosed to have ILD was carried out in Kanchi Kamakoti CHILDS Trust hospital over a period of 2 yr. Infants aged 1 month to 1 yr of age were included if they had (1) respiratory symptoms (Cough, tachypnea or crepitations) for at least 1 month (2) diffuse infiltrates on chest radiography (3) Hypoxemia as defined by oxygen saturation less than 90% by pulse oximetry and (4) High Resolution Computed Tomography (HRCT) of the chest revealing findings of interstitial infiltrates or ground glass pattern. Their case records were analyzed for clinical data, treatment and follow up details. Of the 9 children, who were diagnosed to have ILD, 5 were boys and 4 were girls. The male: female ratio was 1.25: 1. The median age of onset of symptoms was 5 month. The common clinical features observed were tachypnea associated with chest indrawing (100%), cough (100%), hypoxia (100%), failure to thrive and fever (55%) each. The following radiographic patterns were observed in the chest skiagrams: reticulo-nodular pattern in 6(67%) and ground glass pattern in 3(33%). HRCT showed interstitial infiltrates in 6 (67%) and ground glass pattern in 3(33%). Evidence for cyto megalo virus (CMV) infection was detected in 5(56%), Adenovirus in 1 (11%) and Pneumocystis carinii (PCP) in 1(11%) infant. Open lung biopsy was performed in 2 infants, which detected CMV in 1 and PCP in the other. All children received oxygen therapy and systemic corticosteroids (oral/IV) in addition to specific therapy for infection and 3 of these infants succumbed to respiratory failure. CMV Infection was the commonest cause of ILD in infancy in our study. However, the consequences on long term follow up in these infants need to be ascertained.

Reference values for oxygen saturation by pulse oximetry in healthy children at sea level in Chennai

There is little information on oxygen saturation (SaO2) values in children in developing countries. To determine the reference values for oxygen saturation by pulse oximetry in healthy children living at sea level in Chennai and aged between 1 mth and 5 yrs. A prospective study was conducted in Kanchi Kamakoti CHILDS Trust Hospital from February to May 2005. A total of 626 healthy children aged between 1 mth and 5 yrs were examined for heart rate, respiratory rate and SaO2. The mean SaO2 levels for children in the age groups 1-3 mths, 3 mths to 1 yr, 1-3 years and 3-5 years were 98.5%, 98.8%, 98.9% and 99.1%, respectively. The overall mean and median SaO(2) values for the children in the different age groups were 99%. The mean -2 SD values of oxygen saturation in the age groups were 96.5%, 96.4%, 96.3% and 97.1%, respectively, with an overall mean of 96.6%. The reference value for mean SaO2 in healthy children aged between 1 mth and 5 yrs and living at sea level in Chennai city was 98.5% or more, but the -2SD values had a mean of 96.6%.

Infections in burn patients—experience in a tertiary care hospital

A retrospective study was conducted at the Kanchi Kamakoti Childs Trust Hospital to analyze the bacterial isolates from the wounds of patients admitted to the Burns Unit and to determine the sensitivity patterns of the commonly cultured organisms over a 6-year period, December 1998 to December 2004. A total of 535 samples were analysed. Single isolates were more common. Pseudomonas was the most commonly cultured organism followed by Staphylococcus aureus. Escherechia coli and Klebsiella were seen in equal numbers. ESBL rates were 37%. Antibiotic sensitivity patterns and periodic ESBL surveillance helped in selection of appropriate antibiotics.

Profile of pediatric burns: Indian experience in a tertiary care burn unit

Pediatric burns admitted to the tertiary care burn facility of Kanchi Kamakoti CHILDS Trust Hospital in Chennai (India) were retrospectively analysed between 1992 and 2003. Five hundred and thirty-five burn cases were admitted during these years. These children belonged to the age group of 0–18 years (as WHO has increased the pediatric age group range to 0 to 18 years). The etiology of these burns was looked into and the outcome of these patients in respect to etiology and complications were studied. After analysis, they were classified according to age, sex, TBSA and the occurrence of infection during the course of treatment. The complications that really affected the outcome were looked into and infection ranked first in fatal cases. Inhalation burns were not very common in our group and were associated only with large flame burns, which occur when a child is burnt while the mother commits suicide, or in cases of abuse of female children in a closed room with lots of inflammable upholstery. Scalds were the most common type of burn among children under 4 years of age. Flame burns predominated the older age group. Although there were 13 deaths among the entire group, the majority occurred within the 2–4 years age group. There was no significant gender difference with respect to mortality. Large burn size and infection were the strongest predictors of mortality.