In the construction industry, reinforced concrete (R.C.) structures are externally strengthened using bonded steel plates, Carbon Fiber Reinforced Polymer (CFRP), and Glass Fiber Reinforced Polymer (GFRP) laminates. However, these materials' disadvantages include the high price of CFRP and GFRP laminates and the corrosion of steel plates. The alternative for strengthening R.C. structures might therefore be natural fiber-based composite sheets. This study’s goal was to create composite sheets made of local natural fibers (jute fiber), which are cheap, environmentally friendly, and sustainable, and a new local epoxy resin (NOVO BOND EB) with high strength, especially in bonding to concrete, and low cost for use in shear strengthening of R.C. structures. In the experimental programme, the sheets were made from jute fiber-reinforced polymer (JFRP). Three samples of JFRP were tested to determine the mechanical properties of this material. Twelve RC specimens of beams were prepared to study the effect of the variables of laminate manufactured from the JFRP, namely: number of layers (1-2–4), “wrapping configuration (strips – full length sheet) wrapping”, angle of strengthening (90°–45°), and shape (U–U with top cap–box). The proposed strengthened technique of externally bonded reinforcement (JFRP) was compared with a reference sample not strengthened in the shear. The failure patterns were monitored, in addition to recording the failure loads and vertical deflection values. According to the results, JFRP strengthening increased the RC beams' ultimate shear strength by 28 %–131 % when using the strip wrapping technique and by 140 %–175 % when using the full-length wrapping technique. Abaqus (Advanced Tool for Engineering Nonlinear Analysis) was also utilised for finite element analysis. The analysis’s findings agree well with the test findings. Jute textile FRP material has a great deal of potential as a structural strengthening material, as shown by JFRP strengthening. On the verified model, a parametric study was also carried out. Finally, equations for predicting shear strength were employed, and the outcomes of the experiments were compared.