To measure time use of Chlamydia trachomatis screening in non-clinic settings as part of a research study that provides venue-based screening and expedited treatment for 15-24 year old African American men who have sex with women. In a longitudinal, prospective time-motion study, a designated time-keeper observed eight community health workers (CHW) at five sites (two barbershops, two universities, and one job training program) periodically over a two-year period between 2017 and 2018. Ct screening activities were timed for each CHW from initial encounter with participant to completion of screening. Of the 607 screened, 54 men from five sites were followed for the time-motion study. Most (n=41, 76%) were screened at a university while the remaining were screened at either a barbershop (n=9, 17%) or job training program (n=4, 7%). A total of 270 activities were timed. The average time across sites for a CHW to screen a participant was 12 minutes and the average on-site screening staff cost was $4.17 (90% CI: $2.48, $6.35) per person screened. At universities, CHWs spent on average, the shortest time screening men at 11.34 minutes (90% CI: 13.57, 9.11), followed by barbershops and job training sites at 12.45 minutes (90% CI: 18.58, 6.32) and 12.97 minutes (90% CI: 14.39, 10.18), respectively. This resulted in universities having the lowest average on-site screening staff cost of $3.85 (90% CI: $2.29, $5.86) per person screened, followed by barbershops and the job site with average on-site screening staff costs of $4.22 (90% CI: $2.51, $6.43) and $4.40 (90% CI: $2.61, $6.70) per person screened, respectively. STD Screening for African American young men costs were similar across all three venue types studied. While, CHWs appear to be slightly more efficient at universities when screening black men, screenings time costs in all venues were similar.