Background: Job satisfaction among healthcare professionals, particularly senior staff nurses, is essential for maintaining high-quality patient care. The study aims to assess the job satisfaction of senior staff nurses at Rajshahi Medical College Hospital. Method: A descriptive cross-sectional study was select 50 senior staff nurses working in various units at RMCH. Semi-structured interviews were conducted with the selected participants, taking place in private rooms during different shifts. Data collection spanned from November 2014 to April 2015 and covered 40 wards under 7 selected units. Demographic data, including age, gender, religion, educational qualifications, and years of service, were collected from the participants. Job satisfaction-related questions were explored, encompassing aspects such as training, promotion, rewards, job-related factors, feelings towards the job, and overall job experience. Results: The surveyed senior staff nurses were predominantly female (90%), aged 40-49 years (54%). Education levels varied: 68% held nursing diplomas, 24% had B.Sc. in nursing degrees, and 8% possessed MPH qualifications. The majority (34%) had 11-15 years of service, with a minority (4%) having over 25 years. Job Satisfaction Factors: Key job satisfaction drivers included training for skill improvement (80%), promotion opportunities (12%), and recognition through rewards (8%). A significant 92% reported high job satisfaction. Personal motivation (10%), financial incentives (40%), and a desire to help others (50%) played vital roles. Most found their work fulfilling, with 70% stating it fulfilled their desire to assist patients, describing their experiences as rewarding. Conclusion: This study underscores the importance of addressing key determinants of job satisfaction, such as training, promotion, and rewards, to maintain a high level of job satisfaction among senior staff nurses at RMCH. Improving job satisfaction can contribute to a more motivated and effective healthcare workforce, ultimately enhancing patient care.