INTRODUCTION: Telemedicine, the delivery of healthcare services remotely using communication technologies, has rapidly emerged as a transformative solution in the healthcare industry. The successful implementation of any new technology is influenced by several factors, including the knowledge and comprehension of the concept, acquired skills, attitudes towards technology, and the working environment of the professionals involved. AIM: The main objective of this study was to assess the awareness, knowledge, attitude and skills of telemedicine among the health professionals working in various private and government teaching hospitals in Jhansi district of Uttar Pradesh, India. MATERIALS AND METHOD: A cross-sectional survey was conducted among 110 healthcare professionals, including teaching faculties and practitioners from preclinical, para-clinical, and clinical departments in Jhansi district of Uttar Pradesh, India. A pre-validated self-administered questionnaire was used to assess the awareness, knowledge, attitude, and practice of telemedicine. Quantitative data was analyzed using descriptive statistics and the application of the t-test. The study provides valuable insights into the current state of telemedicine among healthcare professionals in the region. RESULTS: The study found that among the respondents, 41% demonstrated good knowledge of telemedicine, while 35% had fair knowledge, and 24% lacked adequate knowledge. Regarding attitude, 39% exhibited a high attitude towards telemedicine, 31% had a moderate attitude, and 30% had a low attitude. In terms of practice, only 9% were skilfully practicing telemedicine on a regular basis, 35% were in learners or beginners stage of practice and 56% were unskilled and incapable of practicing telemedicine and handling related equipments. CONCLUSION: The study findings indicate that while the respondents have limited experience and knowledge in telemedicine technology, a significant proportion of them exhibit a positive attitude towards telemedicine. It is imperative to prioritize the education and training of teaching faculty, practicing physicians, residents, medical students, and other healthcare professionals regarding telemedicine and its associated considerations.