Butterflies are important bio-indicators that should be protected to conserve the biodiversity and environment. They play an important role in the food chain and are valuable pollinators in the local environment. The present study investigated and compared the butterfly abundance and diversity within two different habitats (i) Undisturbed and (ii) Disturbed, in Jawaharlal Nehru Krishi Vishwavidyalaya Campus, Jabalpur Madhya Pradesh. A total of 24 butterfly species were recorded during the study from June 2022 to July 2023 using transects with the aid of sweep nets. An overall total of 2537 butterflies were recorded, which spread across 05 families 17 genera and 23 species. The most abundant family of butterflies caught in undisturbed ecosystems was Pieridae 38% followed by Nymphalidae 27%, Lycaenidae 14%, Papilionidae and Hesperiidae 7%. In disturbed ecosystems butterflies were distributed as Pieridae being dominated with 52% followed by Lycaenidae at 22%, Nymphalidae at 16%, Hesperiidae at 7% and Papilionidae at 3%. The undisturbed habitat was more diversified (H’-1.59) in butterfly diversity than the disturbed habitat (H’- 1.20).