Javanese Thin-Tail Research Articles

Polymorphism and Association of the Novel KCTD2 Gene with Flavor and Odor in Indonesian Local Sheeps

A candidate marker that influences the flavor and odor of Indonesian sheep that is the Potassium Channel Tetramerization Domain Containing 2 (KCTD2) gene. This study aims to examine the polymorphism and association between the KCTD2 gene and lamb flavor and odor in Indonesian local sheeps. This study used DNA taken from the longissimus dorsi (LD) muscle of 100 rams including 75 Javanese Thin-Tail sheep (JTTS), 10 Javanese Fat-Tail Sheep (JFTS), and 15 Jonggol Sheep (JS), with ranged in weight from 20 to 35 kg and were 10 to 12 months old. The PCR-RFLP technique and GLM test analysis were used to identify polymorphisms and association of KCTD2 gene. The restriction enzyme BafI digests the PCR-RFLP product of the KCTD2 gene. The results showed that the KCTD2 gene was polymorphic (CC and CT). KCTD2 gene analysis showed a significant (P<0.05) association with 3-Methylphenol (MP). This research provide information regarding the role of the KCTD2 gene in lamb flavor and odor, especially in 3-Methylphenol (MP) compounds, and explain KCTD2 as a functional gene in the selection of premium sheep with low flavor and odor in lamb meat.

Polymorphisms KIF12 GENE|BfaI and its Association on Carcass Characteristics and Lamb Meat Quality

One of gene which involves on mediation an antioxidant cascade in beta cells as an intracellular target of excess fat intake is Kinesin Family 12 (KIF12). This study aims to identify the diversity of SNP g. 9617965 C>T KIF12 and association with carcass characteristic and meat quality. The total sample used were 102 ram DNA samples consisted of 10 barbados cross sheep (BCS), 52 javanese thin tail (JTT), 15 jonggol sheep (JS), 15 garut sheep (GS), and 10 compass agrinak sheep (CAS). Identification of the diversity of KIF12|BfaI was conducted using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. The results showed that the amplification product was 696 bp. The diversity of the KIF12 gene was found three genotypes namely CC, CT, and TT. The allele frequency in all types of sheep was in Hardy-Weinberg equilibrium. A SNP of the KIF12 was associated (P<0.05) with characteristic of carcass, including length of carcass. The association of KIF12 with meat quality was significant (P<0.05) too associated with pH and water holding capasity (WHC). It could be concluded that KIF12 gene is a potential candidate gene for carcass characteristic and meat quality traits in sheep

In Vitro and in Vivo Anthelmintic Activities of Aqueous Leaf Infusion of Azadirachta indica against Haemonchus contortus

Anthelmintic resistance of Haemonchus contortus worm has become a major problem in ruminant production. Traditional medicinal and potential tropical plants with high tannin contents have a great potency as an alternative source of bio-anthelmintic. The study was carried out to assess the in vitro egg hatches inhibiting (EHI) and adult worm motility (AWM) tests and in vivo anthelmintic activities of aqueous leaf infusion of Azadirachta indica against H. contortus. Leaf infusion at doses of 2%, 4%, and 6% were used for in vitro treatments, and albendazole at a dose of 2 mg/mL was used as a positive control and 0.9% of sodium chloride was used as a negative control. The EHI assay was conducted two times i.e., before treatment and 24 h after treatment. The AWM were monitored 15 and 30 min, and 1, 2, 3, 4, 5, 6, 7, and 8 h post treatment. Sixteen Javanese Thin Tail ewes at the ages of ±12 months that naturally positive of H. contortus with about 1.000 EPG were selected and allocated randomly to 4 groups (n= 4). They were subsequently received the ensuing treatments i.e., A. indica infusion at doses of 0% (A) as a control, 6% (B), 8% (C), and 5 mg/BW albendazole (D) at a single oral dose as a positive control. Experimental ewes in Groups B and C were given oral infusion weekly during 4 weeks of treatment. EPG’s fecal examinations were conducted weekly. The result showed that the leaf infusion of A. indica containing condensed tannin (CT) at a dose of 6% significantly inhibited egg hatching (P<0.05) with a better effect compared to doses of 0%, 2%, and 4%. In AWM test, three doses of infusion significantly difference each other, as well as both negative and positive controls (P<0.05). After treatment, both of the A. indica containing CT levels and albendazole reduced EPG count significantly (P<0.05). Meanwhile, significant difference was not detected in dose of 8% aqueous leaf infusion of A. indica and albendazole treatments on weeks 3, 4, 5, and 6 consecutively. In conclusion, the aqueous leaf infusion of A. indica is fully potential as a bio anthelmintic against H. contortus.

Single Nucleotide Polymorphisms (SNPs) in Exon 6 of Lecithin Cholesterol Acyltransferase (LCAT) Gene in Indonesian Local Sheep

Lecithin cholesterol acyltransferase (LCAT) is a soluble enzyme that converts cholesterol and lecithin to cholesteryl esters and lysolecithins on the surface of high density lipoprotein and plays an important role in lipoprotein metabolism. The research was aimed to explore single nucleotide polymorphisms of LCAT gene in Indonesian local sheep. A total of 118 genomic DNA of Indonesian local sheep were used in this research, consisted of Sumatera Thin Tail (43 heads), Garut (19 heads), Javanese Thin Tail (17 heads), Javanese Fat Tail (6 heads), Rote Island (7 heads), Kissar (7 heads), Sumbawa (10 heads), and Lembah Palu (9 heads). Polymerase chain reaction was used to amplify genomic DNA for exon 6 (250 bp) and direct sequencing method was used to identify polymorphism sequences. The sequences were analyzed with BioEdit and MEGA 5.2 software. The BLAST sequence was obtained from Gene Bank GQ 150556.1. The results showed three novel SNPs, i.e. c.742C&gt;T, c.770 T&gt;A and c.882C&gt;T. Substitution of cytosine to thymine c.742 is a synonymous mutation; thymine to adenine c.770 and cytosine to thymine c.882 are non-synonymous mutations. Polymorphisms of LCAT gene exon 6 was found in Sumatera Thin Tail, Javanese Thin Tail, Javanese Fat Tail, Garut, Lembah Palu, and Rote Island.

IDENTIFICATION OF MYOSTATIN GENE c.960delG LOCUS POLYMORPHISM IN INDONESIAN LOCAL SHEEP BY USING PCR-SSCP METHOD

Myostatin (MSTN) is a protein inhibit muscle growth. This protein is a member of a superfamily ofmolecules called transforming growth factors beta (TGF-b). Deletion in c.960delG (1-bp deletion atposition 960) disrupts the reading frame from amino acid (aa) position 320 to ending in a premature stopcodon in aa position 359 have been found in Norwegian White Sheep. This deletion in the myostatingene is responsible to increase muscle mass, also known as 'double muscling', in sheep. The purpose ofthis study was to identify the polymorphism of myostatin gene in c.960delG locus of local sheep inIndonesia. The 832 DNA sampels from sheep were collected from 13 populations belonging to thePriangan (86), Javanese Thin Tail (389 i.e. Jonggol, Banjar, Ciomas), Javanese Fat Tail (94), West NusaTenggara (136), Rote Island/East Nusa Tenggara (35), Kisar Island/Southwest Maluku (22),Donggala/South East Celebes (45) and Batur cross breed/Wonosobo, Central Java (25). A gene fragmentof MSTN c.960delG length 299 bp was successfully amplified by using the technique of PCR(polymerase chain reaction) and genotyped by SSCP (single strand conformation polymorphism). Theresult showed no polymorphism in this gene. All sheep tested had G/G genotype for c.960delG locus.

Single nucleotide polymorphism in exon two of CBFA1 gene is related to bone mineral denisty.

Duration of oestrus, time of ovulation and hormone profiles for progesterone and LH in prepubertal, pubertal and mature Javanese thin-tail sheep were studied at synchronized oestrus following progestagen-PMSG treatment and at the first natural oestrus after synchronization.The ewe lambs responded to progestagen-PMSG treatment by showing earlier onset of oestrus and an earlier and higher peak of LH concentration than mature ewes. For pre-pubertal, pubertal and mature ewes the mean LH peaks were 49.9, 43.9 and 37.9 ng/ml (P>0.05) at mean intervals of 7.5, 8.4 and 16.5 h (P < 0.05), respectively, after onset of oestrus. Duration of oestrus was 41.2 h in pubertal lambs and averaged 37.5 h in the other two groups (P>0.05). Except in one mature ewe, ovulation occurred between 24 and 36 h after onset of oestrus and the majority ovulated at around the end of oestrus. The corpora lutea developed normally, as indicated by plasma-progesterone changes. The patterns of plasma-progesterone changes were similar in all three groups, though the concentrations were lower in the ewe lambs.At the first natural oestrus after synchronization, mature ewes showed longer (P>0.05) oestrus (31.5 vs. 24.3 h), longer time interval from onset of oestrus to the LH peak (16.0 vs. 12.0 h) and from the LH peak to ovulation (21.0 vs. 19.6 h) than peri-pubertal lambs. Six of eight pre-pubertal lambs did not ovulate at their first natural oestrus, resulting in a conception rate of 11% for that group, while in pubertal lambs and mature ewes conception rates were 70% and 100%, respectively.

Mammary Gland Indices at the End of Lactation in the Superovulated Javanese Thin-Tail Ewes

Mammary Gland Indices at the End of Lactation in the Superovulated Javanese Thin-Tail Ewes

The Effect of Superovulation of Javanese Thin-Tail Ewes Prior to Mating on Lamb Birth Weight and Preweaning Growth

There is little information on optimum management of fodder tree legumes for maximum leaf production. The type of management depends on both their morphology and form of utilisation, which varies between species. As differences exist in relative growth rates and ability to persist over longer periods, their management is influenced by cutting height and cutting frequency (Horne et al., 1986). This study was undertaken at the University of Queensland to observe the responses of commonly used fodder tree legumes to cutting management. Four cutting treatments (60 cm, 100 cm, 150 cm and lopped) commencing in August, 1987 were imposed on five established tree legume species (Acacia angustissimus, Calliandra calothrysus, Albizia chinensis, Gliricidia sepium and Leucaena leucocephala). Each row of species contained five trees per treatment. Two months later the trees were cut at their respective heights and coppiced material lopped and subsequent harvests made at 1.5 monthly intervals until March. Materials harvested were separated into leaf (including flowers and pods) and stems (>6 mm diameter) and oven dried at 60C. The low spring temperatures reduced and inhibited leaf growth of all species and G. sepium respectively, but improved during summer. At 150 cm, L. leucocephala was better during spring and all species produced similar yields during summer. The overall mean leaf yields (%) at the respective heights over the period were 19, 26, 36, 19; 13, 24, 43, 19; 14, 22, 44 and 20. When lopped, leaf yield of C. calothrysus was high during spring and summer but similar to A. chinensis and G. sepium respectively in terms of forage quality. The overall mean leaf yields over the period were 7, 16, 10 and 65%. As cutting height increased, the number of remaining shoots, leaves and buds in the bottom canopy also increased from which regrowth was rapid. However, the proportion of leaves removed either by cutting, lopping or loss through leaf fall removed most photosynthetic tissues and reduced current photosynthate, resulting in reduced leaf yield. Regrowth and plant survival then depended on the reserve carbohydrate in the stem and rate of dry-matter accumulation, which varied between species. The different responses to cutting management provided some options for their use as reserve fodder in grazing systems. Horne, PM Catchpole D W and Ella A 1986 ACIAR

Correlations between lamb birth weight and the concentrations of hormones and metabolites in the maternal serum during pregnancy

Maternal serum hormones (progesterone, oestradiol, triiodothyronine and cortisol) and blood metabolites (β-hydroxy butyric acid [BHBA] and blood urea nitrogen [BUN]) concentrations at weeks 0, 4, 8, 12, 16 and 20 of pregnancy were measured in 39 pregnant Javanese thin-tail ewes (20 and 19 carrying single and multiple [2–3] foetuses, respectively), and six non-pregnant ewes as controls, to evaluate their correlations with lamb birth weight at parturition. All hormones and metabolites changed with the advance of pregnancy. Regression analyses showed that concentrations of triiodothyronine, cortisol, BHBA, and BUN in the maternal circulation during pregnancy had lower correlations with lamb birth weight at parturition than those of progesterone and oestradiol. Progesterone and oestradiol concentrations in the maternal circulation at week 8 of pregnancy had the greatest correlations with lamb birth weight at parturition. The higher the concentrations of progesterone and oestradiol in the maternal circulation at week 8 of pregnancy, the higher the lamb birth weight at parturition. The results suggested that raising maternal serum progesterone and oestradiol concentrations during early pregnancy in sheep, either by exogenous administration or superovulation, might improve prenatal growth and lamb birth weight.

Correlations of Serum Progesterone Concentration with Uterine and Fetal Weights at Weeks 7 and 15 of Pregnancy in Javanese Thin-Tail Ewes

Correlations of Serum Progesterone Concentration with Uterine and Fetal Weights at Weeks 7 and 15 of Pregnancy in Javanese Thin-Tail Ewes

Returns to investments in small ruminant research in Indonesia The Small Ruminant Collaborative Research Support Program (SR‐CRSP) in West Java

AbstractSmall ruminanl research carried out by the SR‐CRSP on management, nutrition and reproduction was developed and evaluated through an Outreach Pilol Program in West Java, Indonesia. On‐farm testing indicated that introduction of different management practices could increase birth rale and survivability of Javanese Thin Tail sheep. This article presents results from an assessment of research investments that measures the potential returns from this technological package, with various adoption and spillover effects, weighs the benefits against the research and training costs of the SR‐CRSP, and looks at distribution of benefits among consumers and producers of sheep in Indonesia. The rate of return in the most conservative scenario was 19%, with potential short‐run benefits exceeding the costs incurred by breeding and nutrition research, and training of 23 masters and doctoral students in 10 years. Market conditions favor producers in the distribution of benefits.

Prediction of Litter Size Based on Hormones and Blood Metabolites Concentrations during Pregnancy in Javanese Thin-Tail Ewes

Prediction of Litter Size Based on Hormones and Blood Metabolites Concentrations during Pregnancy in Javanese Thin-Tail Ewes

Returns to investments in small ruminant research in Indonesia The Small Ruminant Collaborative Research Support Program (SR-CRSP) in West Java

Abstract Small ruminant research carried out by the SR-CRSP on management, nutrition and reproduction was developed and evaluated through an Outreach Pilot Program in West Java, Indonesia. On-farm testing indicated that introduction of different management practices could increase birth rate and survivability of Javanese Thin Tail sheep. This article presents results from an assessment of research investments that measures the potential returns from this technological package, with various adoption and spillover effects, weighs the benefits against the research and training costs of the SR-CRSP, and looks at distribution of benefits among consumers and producers of sheep in Indonesia. The rate of return in the most conservative scenario was 19%, with potential short-run benefits exceeding the costs incurred by breeding and nutrition research, and training of 23 masters and doctoral students in 10 years. Market conditions favor producers in the distribution of benefits.

Maternal serum progesterone concentration during pregnancy and lamb birth weight at parturition in Javanese Thin-Tail ewes with different litter sizes

Pregnant ewes, ( N=38), with similar body weight and age (19, 13 and 6 carrying 1, 2 and 3 fetuses, respectively) were used to study the relationship between maternal serum progesterone concentration during pregnancy and lamb birth weight at parturition. Average maternal serum progesterone concentration in the ewes carrying 1, 2, and 3 fetuses was: 5.3±0.3, 6.2±0.7 and 6.6±0.5 ng/ml, during weeks 0 to 7 of gestation; 16.9±1.4, 25.3±1.5, and 26.8±2.5 ng/ml, during weeks 8 to 20; and 13.2±1.0, 18.7±1.0, and 19.8±1.7 ng/ml, during the entire gestation period respectively. Total lamb birth weight in ewes carrying 1, 2, and 3 fetuses was 1.9±0.1, 3.2±0.2, and 4.2±0.4 kg, respectively. In the respective litter sizes, ewes with higher mean serum progesterone concentration during the whole pregnancy gave birth to lambs with higher birth weight ( r 2=0.76, 0.42 and 0.46, for ewes carrying 1, 2 and 3 fetuses, respectively). The results of the study indicated that prenatal growth could probably be improved by increasing endogenous secretion of maternal progesterone during pregnancy.

Effect of superovulation on maternal serum progesterone concentration, uterine and fetal weights at weeks 7 and 15 of pregnancy in Javanese Thin-Tail ewes

Twenty-one pregnant ewe lambs (nine superovulated and 12 non-superovulated) were used to study the effects of superovulation (injecting 700 IU PMSG at the end of diestrus) on maternal serum progesterone concentrations, uterine and fetal weights at weeks 7 and 15 of pregnancy. In the ewes sacrificed at week 7 of pregnancy, superovulation increased the mean number of corpora lutea ( P<0.01), fetuses ( P<0.01), maternal mean serum progesterone concentration ( P<0.01), mean uterine weight ( P<0.05), total fetal weight ( P<0.01), and average fetal weight ( P<0.01) by 133%, 69%, 354%, 66%, 150% and 40%, respectively, when compared to non-superovulated ewes. In the ewes sacrificed at week 15 of pregnancy, superovulation increased the number of corpora lutea ( P<0.01), fetuses ( P<0.05), maternal serum progesterone concentration ( P<0.01), uterine weight ( P<0.05), total fetal weight ( P>0.05), and average fetal weight ( P<0.05) by 207%, 20%, 84%, 37%, 29% and 24%, respectively, compared to those non-superovulated ewes. It was concluded that the increased number of corpora lutea and, therefore, their hormonal secretions by superovulation could increase uterine and fetal growth and development.

Correlations of litter size and maternal serum progesterone concentration during pregnancy with mammary gland growth and development indices at parturition in Javanese Thin-Tail sheep

Correlations of litter size and maternal serum progesterone concentration during pregnancy with mammary gland growth and development indices at parturition in Javanese Thin-Tail sheep

The use of Wonnolas in controlling gastrointestinal nematode infections in sheep under traditional grazing management in Indonesia

Blocks containing 3% phenothiazine in solidified molasses (Wonnolas, Animeal Australia Ltd .) were tested to control gastrointestinal nematode infections and the effect on mineral status in sheep in villages in Cirebon, Indonesia. Two hundred and thirteen Javanese Thin Tail sheep which were grazed during the day and housed at night were divided into two groups on the basis of location . One group was allowed in an access to Wonnolas blocks when penned for 24 weeks while the other group remained untreated. The bodyweight was recorded and faecal samples were collected for egg counts and larval culture every four weeks. Sera and saliva samples were collected from 20 adult sheep in each group at weeks 0, 12 and 24 for mineral analysis by AAS and colorimetry. The results indicated that the mean egg counts of the treated group decreased from 576 epg to 123 epg and the percentage of sheep producing viable larvae decreased from 50% to 24%. In contrast egg counts of the control group increased from 768 epg to 4,840 epg and the percentage of sheep producing viable larvae increased from 65% to 84% over the same period . In the treated group the number of Haenronchus larvae declined significantly (36% to 80%) . Mineral analysis revealed deficiencies in sodium and copper, low levels of zinc and normal levels of potassium, calcium, magnesium and phosphorus . Wormolas had a significant effect on sodium and zinc status but not on copper although sufficient levels of this element were available. Comparison of bodyweight gains showed a significantly (P<0.05) higher rate of increase in the treated animals.

Effects of energy supplementation on lamb production of Javanese thin-tail ewes.

A 3-yr study was conducted in North Sumatra, Indonesia, as part of an evaluation of the feasibility of integrating sheep and rubber production. The objective was to evaluate the effects of increasing energy supplementation on reproduction and other performance criteria of Javanese Thin-Tail sheep grazing volunteer forages under 8- and 14-yr-old rubber trees. The control group was unsupplemented. The medium group was supplemented with high-energy feeds at 1% of the flock body weight, with the low and high groups receiving 60% or 140% of the daily energy provided by the medium group diet. Supplements provided 1.2 g protein per kilogram BW. There were 158 lambs born to the 152 ewes in the 1st year of the study. Preweaning mortality rates of lambs were reduced (P less than .01) with supplementation (45 vs 12, 3 and 12% for the control, low, medium and high groups, respectively). During the 3 yr, litter size was higher (P less than .01) in the high group (1.33, 1.31 and 1.34 vs 1.71 for ewes on the four respective diets). Observed repeatability of litter size of individual ewes in all treatment groups for the first three parities was higher (P less than .01) than would be expected if litter size were a random event. Of the lambs born in the 1st yr, kilograms of lamb weaned per ewe joined were 3.1, 7.8, 7.3 and 9.8. At prevailing prices, only the high supplement level was profitable compared to the control. For the high group, the added return from the sale of lambs born the 1st yr was 120% of the added cost of supplementing the ewes until all the lambs were weaned (15 mo). Response of sheep to the high level of energy supplementation, especially with regard to litter size, indicates that supplementing sheep grazing in rubber plantations at a high level can be profitable.

A pragmatic approach to improving small ruminant diets in the Indonesian humid tropics.

Research conducted since 1980 on the nutrition and feeding of Kacang goats and Javanese Thintail sheep for meat production in Indonesia is reviewed. Feeding practices in three villages were monitored, along with animal production criteria, to better understand the limitations imposed by feed resource availability. Village and experiment station data showed that native or cultivated tropical grasses fed alone did not support growth of young goats and that lambs fed napier grass alone grew at no more than 20 to 25 g/d. Supplementing these grasses with tree legume foliage or wilted cassava leaves at modest levels improved ADG to about 50 g for lambs and 20 g for kids. Faster gains were obtained with higher intakes of tree legume foliage, or with combinations of higher-energy supplements such as rice bran, cassava meal and molasses. A response to mineral supplementation also was shown. Reproductive performance of North Sumatran ewes grazing a native pasture under rubber trees also was improved with supplementation. These experiments show that economically effective options exist for improving small ruminant diets in the humid tropics.

Oestrous cycle dynamics in peri-pubertal and mature Javanese thin-tail sheep

Duration of oestrus, time of ovulation and hormone profiles for progesterone and LH in prepubertal, pubertal and mature Javanese thin-tail sheep were studied at synchronized oestrus following progestagen-PMSG treatment and at the first natural oestrus after synchronization. The ewe lambs responded to progestagen-PMSG treatment by showing earlier onset of oestrus and an earlier and higher peak of LH concentration than mature ewes. For pre-pubertal, pubertal and mature ewes the mean LH peaks were 49.9, 43.9 and 37.9 ng/ml ( P>0.05) at mean intervals of 7.5, 8.4 and 16.5 h ( P < 0.05), respectively, after onset of oestrus. Duration of oestrus was 41.2 h in pubertal lambs and averaged 37.5 h in the other two groups ( P>0.05). Except in one mature ewe, ovulation occurred between 24 and 36 h after onset of oestrus and the majority ovulated at around the end of oestrus. The corpora lutea developed normally, as indicated by plasma-progesterone changes. The patterns of plasma-progesterone changes were similar in all three groups, though the concentrations were lower in the ewe lambs. At the first natural oestrus after synchronization, mature ewes showed longer ( P>0.05) oestrus (31.5 vs. 24.3 h), longer time interval from onset of oestrus to the LH peak (16.0 vs. 12.0 h) and from the LH peak to ovulation (21.0 vs. 19.6 h) than peri-pubertal lambs. Six of eight pre-pubertal lambs did not ovulate at their first natural oestrus, resulting in a conception rate of 11% for that group, while in pubertal lambs and mature ewes conception rates were 70% and 100%, respectively.