Japonica Fruit Research Articles

UPLC/HR-ESI-MS/MS and GC/MS profiling of Eriobotrya japonica L. fruit in correlation to its antioxidant, anti-inflammatory, and anti-arthritic effects.

Eriobotrya japonica Lindl. (Loquat) fruit is a subtropical edible fruit originally from China. It grows well in Egypt, but it is not widely known. In the current study, the fruit was extracted with 80% ethanol to get the total ethanol extract (TEE). A part of which was fractionated by dichloromethane to yield polar and nonpolar fractions (PF and NPF). The antioxidant and anti-inflammatory activities of the TEE were in vitro evaluated. The complete Freund's adjuvant (CFA) arthritis model was used to explore the in vivo biological assessment of the anti-arthritic properties in vivo of the TEE, PF, and NPF of the fruit. Additionally, the inspected limbs detached from all animals were subjected to histological inspection. Moreover, GC/MS analysis of the unsaponifiable (USF) and saponifiable (SF) fractions of the NPF was performed. Furthermore, 64 metabolites from various chemical classes were identified using UHPLC/HR-MS/MS analysis of the TEE of the fruit in both positive and negative ionization modes. The positive ionization mode of loquat fruit allowed for the first time the detection of two kinds of lyso-glycerophospholipids (Lyso-GPLs): lyso-glycerophosphoethanolamines (Lyso-PtdEtn) and lyso-glycerophosphocholines (Lyso-PtdCho). The fruit extracts exhibited a notable in vivo anti-arthritic activity by decreasing paw thickness in the treated rats and adjusting the inflammatory mediators. The TEE showed the highest anti-arthritic activity, followed by the PF that showed an observed activity, while the NPF exhibited the lowest activity. Histopathological findings showed a marked improvement in the arthritic condition of the excised limbs. Thus, E. japonica fruit may be considered as a promising natural antioxidant and anti-arthritic agent.

Inactivation effect of extracts of gardenia fruit, licorice and Torilis japonica fruit against Bacillus spores

Inactivation effect of extracts of gardenia fruit, licorice and Torilis japonica fruit against Bacillus spores

Experimental study of solar drying kinetics and evaluation of activation energy of Eriobotrya japonica fruits

AbstractGiven the seasonal nature and high perishability of Eriobotrya japonica fruits, employing effective preservation techniques like drying is crucial to minimize economic losses and extend the fruit's availability throughout the year. This study rigorously examines the drying kinetics of Eriobotrya japonica fruits using an indirect forced convection solar dryer and highlights the technological advantages of utilizing this dryer over traditional open‐air methods, showing that controlled conditions provide improved drying rates. The research develops the equation for the characteristic drying curve. The Midilli‐Kucuk model was demonstrated to be the most appropriate for describing these kinetics under various conditions, offering a reliable method for predicting drying behavior at untested temperatures. The thermal diffusivity was determined and rises as the temperature increases. The application of the Arrhenius approach enabled the determination of an activation energy of 17.79 kJ/mol, confirming the substantial impact of temperature on the drying process.Practical applicationsThe global challenge of food preservation, especially for perishable fruits like Eriobotrya japonica, requires innovative and efficient solutions to minimize economic losses and ensure the year‐round availability of nutritious produce. Our research demonstrates the technological advantages of using a forced convection solar dryer over traditional methods for drying E. japonica. This system maintains a consistent air temperature, ensuring a stable energy supply, faster, and more uniform drying. A major contribution of this study is the development of the characteristic drying curve equation, which is crucial for optimizing drying parameters and designing an efficient solar dryer specifically for E. japonica. This research not only advances the understanding of the drying kinetics and thermal properties of E. japonica but also provides a foundation for the development of more efficient and sustainable drying technologies. By optimizing the drying process, this study contributes to improving the preservation and quality of loquat fruits, thereby supporting agricultural sustainability and reducing post‐harvest losses. This work is highly relevant to the broader energy community, as it showcases the potential of solar drying technologies in enhancing food preservation while promoting the use of renewable energy sources.

Effect of Torilis japonica Fruit Extract for Endothelium-Independent Vasorelaxation and Blood Pressure Lowering in Rats.

Torilis japonica (TJ) fruit, is a herb that is traditionally used for erectile dysfunction (ED). Given the shared mechanisms of ED and hypertension through vascular smooth muscle, we hypothesized that TJ would be effective in vasodilation and blood pressure reduction. This study confirmed the authenticity of TJ samples via DNA barcoding and quantified the main active compound, torilin, using HPLC. TJ was extracted with distilled water (TJW) and 50% ethanol (TJE), yielding torilin contents of 0.35 ± 0.01% and 2.84 ± 0.02%, respectively. Ex vivo tests on thoracic aortic rings from Sprague-Dawley rats showed that TJE (3-300 µg/mL) induced endothelium-independent, concentration-dependent vasodilation, unlike TJW. Torilin caused concentration-dependent relaxation with an EC50 of 210 ± 1.07 µM. TJE's effects were blocked by a voltage-dependent K+ channel blocker and alleviated contractions induced by CaCl2 and angiotensin II. TJE inhibited vascular contraction induced by phenylephrine or KCl via extracellular CaCl2 and enhanced inhibition with nifedipine, indicating involvement of voltage-dependent and receptor-operated Ca2+ channels. Oral administration of TJE (1000 mg/kg) significantly reduced blood pressure in spontaneously hypertensive rats. These findings suggest TJ extract's potential for hypertension treatment through vasorelaxant mechanisms, though further research is needed to confirm its efficacy and safety.

Loquat (Eriobotrya japonica) fruit consumption and preference by cloud forest birds: Implications for woody native species regeneration

Secondary forests from abandoned shade coffee plantations are commonly found close to Neotropical cities. These peri-urban forests provide important environmental services; however, their abandonment and proximity to the city can promote an increase of exotic species that could potentially change the structure and function of the system by creating novel ecosystems. The loquat (Eriobotrya japonica) is a tree native to Asia and is recognized as a potentially invasive species in the tropical montane cloud forest region of Central Veracruz, Mexico. We analyzed the consumption of and preference for loquat fruits in cloud forest frugivorous birds. In November 2020, we established six feeding stations with camera traps in a peri-urban cloud forest fragment in Xalapa, Mexico. To determine which bird species could consume and disperse E. japonica, fruits of this and another four native species (Psychotria nervosa, Hoffmannia excelsa, Picramnia antidesma, and Xylosma flexuosa) were exposed separately. After 444 camera-days, we recorded eight frugivorous bird species, seven of which consumed E. japonica fruits. Chlorospingus flavopectus was the only species to present a level of loquat fruit consumption greater than that expected by chance. We conducted experiments to assess whether the birds preferred to consume E. japonica fruits compared to those from three native species (Cestrum miradorense, P. nervosa, and X. flexuosa) that were offered simultaneously. All bird species exclusively consumed E. japonica fruits when contrasted against P. nervosa or X. flexuosa. The only native species fruits that were consumed were those of C. miradorense, although the birds also preferred to consume loquat fruits rather than this native species. The loquat therefore represents an important food resource and could act to influence the consumption and dispersal of native cloud forest plant species. The implications for cloud forest management are discussed.

РОЛЬ МИКРОВОЛНОВОГО И УЛЬТРАЗВУКОВОГО ИЗЛУЧЕНИЯ В ПРОЦЕССЕ ЭКСТРАКЦИИ ФЛАВОНОИДОВ ИЗ ПЛОДОВ СОФОРЫ ЯПОНСКОЙ

The influence of ultra-high-frequency and ultrasonic radiation on the process of extraction of flavonoids from Sophora japonica fruits was studied. Treatment with ultra-high-frequency and ltrasonic radiation was carried out after swelling of pre-crushed Sophora japonica fruits in the extractant. Then the flavonoids were extracted by maceration and heating. The amount of flavonoids in the extract was determined by photocolorimetry. It was shown that the yield of flavonoids depends on the duration and method of exposure and on the extraction method. The highest yield after xposure to ultra-high-frequency and ultrasonic radiation is achieved by heating. The processing time corresponding to the maximum yield of flavonoids was determined

Chromatographic and Biological Screening of Chosen Species of Schisandraceae Family: Schisandra chinensis, S. rubriflora, S. sphenanthera, S. henryi and Kadsura japonica.

HPLC and TLC profiling was carried out for leaf and fruit extracts of five Schisandraceae species: Schisandra chinensis, S. rubriflora, S. spehenanthera, S. henryi and Kadsura japonica. HPLC measurements confirmed presence of lignans and phenolic compounds in fruits and leaves of all tested species. The most abundant in lignans was S. chinensis fruit extract in which 15 compounds were detected (e. g.: schisandrol A, schisanhenol, γ-schisandrin, gomisin N). The effect-directed detection, i. e., TLC-direct bioautography against Bacillus subtilis, showed exceptionally high activity for S. chinensis and S. rubriflora fruit extracts. On the other hand, TLC-DB enzyme tests (α-glucosidase, lipase, tyrosinase and acetylcholinesterase (AChE) inhibition assays) showed that all fruit and leaf extracts have ability to inhibit the above-mentioned enzymes (except for the K. japonica fruit). The leaf extracts showed much stronger antioxidant activity than the fruit ones, which were assessed and compared using both TLC-direct bioautography and spectrophotometric measurements based on ABTS, DPPH and FRAP tests.

Antioxidant and Antiproliferative Activities of Phenolic Extracts of Eriobotrya japonica (Thunb.) Lindl. Fruits and Leaves.

Increasing interest in new sources of secondary metabolites as biologically active substances has resulted in an advanced study of many plant species. Loquat (Eriobotrya japonica (Thunb.) Lindl. = Rhaphiolepis bibas (Lour.) Galasso & Banfi, Rosaceae family), an evergreen, subtropical fruit tree, native to China and Japan, but cultivated in southern countries of Europe, is a species commonly used in folk medicine and may be an excellent source of bioactive compounds. Therefore, the aim of the present study was to evaluate the profile of the phenolic constituents of E. japonica fruits and leaves originating from Tuscany (Italy), as well as their in vitro antioxidant and chemopreventive activities on human cancer cell lines breast adenocarcinoma (MCF-7), colon adenocarcinoma (Caco-2 and HT-29), and glioblastoma (U87MG). Results revealed that the extract of leaves displayed higher antioxidant and anticancer potential than the fruit extract and contained 25 individual phenolic compounds that have been characterized and quantified by the UPLC-PDA-MS method. The antiproliferative activity was correlated with the content of polyphenolic compounds indicating that both fruits and leaves are a good source of antioxidants and may be exploited as nutraceuticals enriching food or as components for the cosmetic/pharmaceutical industry.

Dried Loquat Fruit Extract Containing Chlorogenic Acid Prevents Depressive-like Behaviors Induced by Repeated Corticosteroid Injections in Mice.

Eriobotrya japonica (loquat tree) has been used in traditional medicine to treat respiratory ailments, inflammation, and skin diseases; however, its potential antidepressant-like effects have not been extensively investigated. In this study, we evaluated the antidepressant-like effects of E. japonica fruit extract (EJFE) in a mouse model of corticosterone (CORT)-induced depression. An HPLC analysis revealed that chlorogenic acid (CGA) is the major compound in EJFE. Male ICR mice (5weeks-old) were injected with CORT (40 mg/kg, intraperitoneally) once daily for 21 days to induce depressive-like behaviors. Various behavioral tests, including the open field test, rotarod test, elevated plus maze (EPM), passive avoidance test (PAT), tail suspension test (TST), and forced swim test (FST), were conducted 1 h after the oral administration of EJFE at different doses (30, 100, and 300 mg/kg) and CGA (30 mg/kg). High-dose EJFE and CGA significantly alleviated CORT-induced depressive-like behaviors, as indicated by the reduced immobility times in the TST and FST. A decrease in the step-through latency time in the PAT, without an effect on locomotor activity, suggested an improvement in cognitive function. Moreover, EJFE- and CGA-treated mice exhibited significantly reduced anxiety-like behaviors in the EPM. Our results imply the promising potential of EJFE containing CGA as a therapeutic candidate for depression.

A Review on Torilis japonica: Ethnomedicinal, Phytochemical, and Biological Features.

The present study was aimed at comprehensive overviewing a phytochemically and biologically important species namely Torilis japonica (Apiaceae family). Treatment of dysentery, fever, haemorrhoids, spasm, uterine tumors, lymphadenitis, rheumatism, impotence, infertility, women's diseases, and chronic diarrhea are reported as the main folk medicinal applications of the T. japonica fruits. So far, the plant is phytochemically characterized for its diverse terpene derivatives, predominantly sesquiterpenes. The plant's fruit is a rich source of torlin, a guaiane-type sesquiterpene, possessing various potent bioactivities. To date, anticancer, anti-inflammatory, antimicrobial, antioxidant, skin photoaging activities of the plant extracts and its constituents have been evaluated. Further investigation of the plant, specifically bioassay-guided isolation and identification of its major bioactive constituents can lead to discover potential phytopharmaceutical candidates.

Assessment of the anti-hair loss potential of Camellia japonica fruit shell extract in vitro.

Hair loss is caused by various factors. Impacts of these factors are often overlapped and intensified. Currently, mitigation of hair loss is being studied by proliferating dermal papilla cells (DPCs) and inhibiting deleterious factors such as dihydrotestosterone (DHT) and oxidative stress on hair growth. Camellia japonica (C.japonica) fruit shell is a discarded part. Its biological activity remains to be elucidated. In this study, we investigated the capacity of C.japonica fruit shell extract (CJFSE) for hair loss mitigation. MTT assay, spheroid culture and quantitative RT-PCR were performed to observe the proliferative effect of CJFSE on hair follicle dermal papilla cells (HFDPCs). Effects of CJFSE on DHT-induced hair loss were confirmed by Dkk-1 ELISA, β-galactosidase (β-gal) and 5α-reductase activity assay. In addition, effects of CJFSE on oxidative stress were confirmed through DPPH and ROS production assays. CJFSE increased the proliferation and spheroid size of HFDPCs. Expression levels of VEGF-A, Wnt-1, c-Myc and Cyclin D1 were upregulated by CJFSE. CJFSE also suppressed 5α-reductase activity and DHT-induced decrease in cell proliferation, Dkk-1 secretion and β-gal activity. Moreover, CJFSE showed DPPH scavenging activity and ameliorated hydrogen peroxide-induced ROS production and β-gal activity. Finally, gallic acid and protocatechuic acid were observed in CJFSE through HPLC analysis. CJFSE has the potential to alleviate hair loss by promoting hair cell growth and suppressing effects of DHT and oxidative stress on hair.

Improvement of Immune and Hematopoietic Functions by Litsea japonica Fruit Extract in Cyclophosphamide-Treated BALB/c Mice

Loss of immunity is an important cause in the pathology of infectious disease. This study investigates the effect of Litsea japonica fruit extract (LJFE) as a potential functional food on immunity and hematopoietic function in immunosuppressed BALB/c mice. Immunity-stimulating activity was observed in mice supplemented with LJFE at low (25 mg/kg), medium (50 mg/kg), and high (100 mg/kg) dosage for seven days after administration of cyclophosphamide. LJFE treatment significantly improved spleen injury score (p < 0.001) and body weight (p < 0.02) by approximately two-fold with a high dosage of LJFE (100 mg/kg). Spleen-derived lymphocyte analysis demonstrated that the numbers of clusters of differentiation (CD)4+ and CD8+ T-cells were notably increased by approximately two-fold (p < 0.001) with a high dosage of LJFE (100 mg/kg). In mouse splenocytes differentiated into T- and B-lymphocytes, LJFE significantly induced proliferation up to approximately 90% of control for T- (p < 0.001) and B-lymphocytes (p < 0.01) with a high dosage of LJFE (100 mg/kg). Furthermore, LJFE significantly recovered the numbers of white blood cells, red blood cells, and platelets. Enzyme-linked immunosorbent assay revealed that serum levels of immune-related cytokines, such as tumor necrosis factor-α, interleukin (IL)-1β, IL-6, IL-2, and interferon (IFN)-γ, were notably Increased. In addition, serum levels of immunoglobulin (Ig) A, IgM, and IgG were restored by LJFE treatment. This study provides a reference to use L. japonica as a functional food ingredient to improve immunity and hematological function in humans.

까마귀쪽나무 열매 분말을 첨가한 스콘의 이화학적 품질 특성

This study examined the quality characteristics and antioxidant activities of scones prepared using Litsea japonica fruit powder (LJFP). The density of scone dough with LJFP was 1.07-1.13 g/mL, and the pH of scone dough was the highest in LJFP0. Baking loss rate, volume, and soluble solid content of scones did not differ among samples, but moisture content was the highest in LJFP0. In terms of color, L* and b* values of scones decreased and a* value increased with increasing LJFP content. The texture properties revealed that hardness, cohesiveness, gumminess, and chewiness but not springiness of scones decreased with the addition of LJFP. The total polyphenol contents, total flavonoid contents, and the DPPH and ABTS+ radical scavenging activities of scones were determined to increase with increasing LJFP contents. Reducing power varied from 0.53-1.32. Based on a sensory evaluation test, the overall preference, flavor, and texture were the lowest in LJFP0. The appearance and color of scones showed higher scores in LJFP0 and LJFP1, and taste was the highest in LJFP1. These results suggest that adding 0.625-2.5% LJFP is optimal for making scones.

Efficient decolorization of cationic dye (malachite green) by natural-based biosorbent (nano-magnetic Sophora Japonica fruit seed biochar)

Today, the importance of materials produced by natural means or by synthesis is increasing in the prevention of increasing water pollution. Sophora Japonica fruit seeds (SJfs) were pyrolyzed at 450 °C to produce SJfs-biochar (SJfsB) in this study. Iron nanoparticles were immobilized to the SJfsB structure to form a more active biosorbent matrix. SJfsB and the resulting novel biosorbent (nanomagnetic-Sophora Japonica fruit seeds biochar (nM-SJfsB) were used to remove malachite green (MG) from an aqueous solution. Fourier transform infrared analysis (FTIR) and scanning electron microscopy were used to elucidate the details of the biosorbent properties. The effect on removal was investigated using various parameters. A combined strategy was used to define the adsorption parameters such as adsorbent amount, time, initial dye concentration, pH, and temperature. The maximum adsorption capacity determined by the Langmuir model was 51.020 mg/g for SJfsB-MG and 101.010 mg/g was found for nM-SJfsB-MG. The pseudo second order equation made the equilibrium data suitable. After regeneration, nM-SJfsB can be used for re-adsorption studies and reused for multiple cycles in adsorption and desorption applications without discharging nano-iron particles into aqueous medium.

First report of Colletotrichum scovillei causing anthracnose fruit rot on Eriobotrya japonicain in Zhejiang province, China.

Loquat (Eriobotrya japonica (Thunb.) Lindl.) is a fruit tree of high economic impotance in China. In May 2021, fruit rot on cv. "Baozhu" was observed in Yuhang district (119°40'E, 30°09'N), Zhejiang province, at an incidence of more than 30% within five orchards (the total affected area were about 121, 000 m2). Early symptoms of naturally affected fruits were dark brown, necrotic lesion (Fig. 1 A). Lesions subsequently expanded, and orange conidia were observed (Fig. 1 B). Conidia were transferred using a sterilized needle into sterile water, diluted to several conidia (approximately 10 conidia/µL) in the field of light microscope, and spread onto potato dextrose agar (PDA). After 24 h, single colonies were transferred, and six single-spore strain isolated from different fruits or locations were obtained. Six-day old colonies grown on PDA at 27°C had gray-green centers with white edges, and on the reverse side, the centers were brown (Fig. 1 C). Isolate PPGS2 was selected for further characterization. Conidia were unicellular, smooth-walled, hyaline, cylindrical with one rounded and one acute end, or with both ends rounded and they (n = 30) measured 11.1 to 16.0 µm (mean = 13.3 µm) × 3.0 to 4.6 µm (mean = 4.1) (Fig. 1 D). Appressoria were single or in smalln groups, light brown to dark brown, ovoid or elliptical with a smooth or undulate outline, and measured (n = 30) 4.6 to 8.7 µm (mean = 6.5 µm) × 4.0 to 5.6 µm (mean = 4.9 µm) (Fig. 1 H). These features of PPGS2 were consistent with species of the Colletotrichum acutatum species complex (Damm et al. 2012). For species identification, the internal transcribed spacer (ITS), beta-tubulin (TUB), chitin synthase (CHS-1), calmodulin (CAL), and actin (ACT) genes were sequenced (O'Donnell et al. 2000; Weir et al. 2012). The five sequences were deposited in GenBank (OK054581, OK077960, OK077987, OK077988 and OK077989, respectively). A BLAST search of these sequences showed 99% identity with sequences of the ex-holotype C. scovillei CBS126529 (Damm et al. 2012). The evolutionary tree shown that PPGS2 and C. scovillei CBS126529 were clustered in a branch (SH-aLRT/approximate Bayes test/bootstrap support = 88.6/0.999/96). Combining morphological characteristics with phylogenetic analysis, PPGS2 was identified as C. scovillei. To fulfill Koch's postulates, E. japonica fruit were disinfected with 75% alcohol and air-dried for 5 mins to allow the alcohol to volatilize. A conidial suspension (10 mL of 1 × 106 conidia/ml) of PPGS2 was sprayed onto six fruits, and six fruit sprayed with sterilized water served as controls. The experiment was repeated three times. Fruits were kept on a mist bench at 27°C and 80% relative humidity for 5 days. Inoculated fruit developed dark brown necrotic lesions that later fused and expanded (Fig. 1, E-G), whereas control fruit remained symptomless. C. scovillei was re-isolated from lesions of inoculated fruit. C. scovillei has been reported from fruit of Capsicum sp., Musa sp., Mangifera indica, and Clausena lansium in China (Farr and Rossman 2021). To our knowledge, this is the first report of C. scovillei causing anthracnose fruit rot of E. japonica in China. This disease results in severe economic losses both in the field and after harvest, and it is necessary to develop more effective prevention and control strategies.

Kadsura japonica fruits exert immunostimulatory and anti-obesity activity in RAW264.7 and 3T3-L1 cells

ABSTRACT Under the COVID-19 pandemic, interest in immune enhancement and anti-obesity is increasing. Thus, in this study, we investigated whether Kadsura japonica fruits (KJF) exhibits immunostimulatory activity and anti-obesity activity. KJF increased the production of immunostimulatory factors and phagocytosis in RAW264.7 cells. Inhibition of TLR2 and TLR4 blocked KJF-mediated production of immunostimulatory factors in RAW264.7 cells. In addition, the inhibition of MAPK and PI3 K/AKT signaling pathway reduced KJF-mediated production of immunostimulatory factors, and the activation of MAPK and PI3 K/AKT signaling pathway by KJF suppressed the inhibition of TLR2/4. KJF attenuated the lipid accumulation and the protein expression such as CEBPα, PPARγ, perilipin-1, adiponectin, and FABP4 related to the lipid accumulation in 3T3-L1 cells. In addition, KJF inhibited excessive proliferation of 3T3-L1 cells and protein expressions such as β-catenin and cyclin D1 related to cell growth. These findings indicate that KJF may have immunostimulatory activity and anti-obesity activity.

C-CorA: A Cluster-Based Method for Correlation Analysis of RNA-Seq Data

Correlation analysis is a routine method of biological data analysis. In the process of RNA-Seq analysis, differentially expressed genes could be identified by calculating the correlation coefficients in the comparison of gene expression vs. phenotype or gene expression vs. gene expression. However, due to the complicated genetic backgrounds of perennial fruit, the correlation coefficients between phenotypes and genes are usually not high in fruit quality studies. In this study, a cluster-based correlation analysis method (C-CorA) is presented for fruit RNA-Seq analysis. C-CorA is composed of two main parts: the clustering analysis and the correlation analysis. The algorithm is described and then integrated into the MATLAB code and the C# WPF project. The C-CorA method was applied to RNA-Seq datasets of loquat (Eriobotrya japonica) fruit stored or ripened under different conditions. Low temperature conditioning or heat treatment of loquat fruit can alleviate the extent of lignification that occurs because of postharvest storage under low temperatures (0 °C). The C-CorA method generated correlation coefficients and identified many candidate genes correlated with lignification, including EjCAD3 and EjCAD4 and transcription factors such as MYB (UN00328). C-CorA is an effective new method for the correlation analysis of various types of data with different dimensions and can be applied to RNA-Seq data for candidate gene detection in fruit quality studies.

ASSESSMENT OF ANTIOXIDANT ACTIVITY, TOTAL PHENOLICS, AND FLAVONOIDS OF DIFFERENT SOLVENT FRACTIONS OF Eriobotrya japonica Lindl. FRUIT EXTRACT

The purpose of the study was to determine the antioxidant potential, total phenol, and total flavonoid content in the nhexane fraction, ethyl acetate fraction, and water fraction of Eriobotrya japonica fruit. The research method for testing the antioxidant activity of the fruit of Eriobotrya Japonica was the DPPH (1.1-diphenyl-2-picryhydrazil ) method by UV-VIS spectrophotometry, while phenol and flavonoids contents were carried out by colorimetric method UV-Vis spectrophotometry. The determination of total phenol with Folin-Ciocalteu reagent and gallic acid as the standard, and the determination of total flavonoid content with AlCl3 reagent and quercetin as the standard. The results revealed that the IC50 values for the antioxidant activity of the n-hexane fraction, ethyl acetate fraction, and water fraction are 170.21, 68.33, and 351.95 g/mL, respectively. The total phenol concentration in the n-hexane fraction, ethyl acetate fraction, and water fraction, respectively, was 13.08, 95.34, and 15.24 mg GAE/g of material. The n-hexane fraction, ethyl acetate fraction, and water fraction contained 0.88, 12.65, and 2.6 mg QE per gram of material, respectively. Compared to the n-hexane and water fractions, the ethyl acetate fraction of Eriobotrya Japonica fruit had the highest quantities of total phenol and total flavonoid.

Soluble cuticular wax composition and antimicrobial activity of the fruits of Chaenomeles species and an interspecific hybrid

Plants of the genus Chaenomeles Lindl. (Rosaceae) naturally grow in Southeast Asia and represent the richest resource of biologically active compounds with beneficial properties for humans. Plants of C. japonica (Thunb.) Lindl. and C. speciosa (Sweet) Nakai species, and interspecific hybrid C. × superba (Frahm) Rehder (C. japonica × C. speciosa, Superba group) have been successfully introduced in the steppe zone of Ukraine and bear fruits. In this study, we evaluated chemical composition of fruit cuticular waxes and antimicrobial activity of fruit extracts. The soluble waxes were characterized using gas chromatography-mass spectrometry (GC-MS), and 26–36 compounds, representing 91.7–96.6% of the total soluble cuticular waxes, were identified. Waxes of Chaenomeles fruits belonged to six classes, namely fatty acids, alcohols, aldehydes, esters, ethers and alkanes. Aldehydes 7-hexadecenal and heptacosanal, and alkanes hexatriacontane and tetrapentacontane were the main constituents in the soluble cuticular waxes of C. speciosa and C. × superba fruits, accounting for more than half of the total contents. However, alkane tetrapentacontane, alcohol 8,10-hexadecadien-1-ol and heptacosanal prevailed in C. japonica fruit waxes. Isopropanolic fruit extracts exhibited dose-dependent antimicrobial activity against four Gram-negative bacteria, five Gram-positive bacteria and one fungal strain in the disc diffusion assay. In general, extracts from the Chaenomeles fruits demonstrated higher activity against Gram+ bacteria than Gram- strains. The strongest inhibiting activity was shown against Staphylococcus epidermidis (by the fruit extracts of C. × superba and C. speciosa), Micrococcus lysodeikticus and Candida albicans (both by C. × superba fruit extract). Results of the study confirmed accumulation of the bioactive compounds in the fruit waxes of different Chaenomeles species and antimicrobial ability of Chaenomeles fruits as well. These findings revealed the bioactive compounds in fruit cuticular waxes and suggested health-promoting properties of introduced Chaenomeles species.

Soluble cuticular wax composition and antimicrobial activity of the fruits of Chaenomeles species and an interspecific hybrid

Plants of the genus Chaenomeles Lindl. (Rosaceae) naturally grow in Southeast Asia and represent the richest resource of biologically active compounds with beneficial properties for humans. Plants of C. japonica (Thunb.) Lindl. and C. speciosa (Sweet) Nakai species, and interspecific hybrid C. × superba (Frahm) Rehder (C. japonica × C. speciosa, Superba group) have been successfully introduced in the steppe zone of Ukraine and bear fruits. In this study, we evaluated chemical composition of fruit cuticular waxes and antimicrobial activity of fruit extracts. The soluble waxes were characterized using gas chromatography-mass spectrometry (GC-MS), and 26–36 compounds, representing 91.7–96.6% of the total soluble cuticular waxes, were identified. Waxes of Chaenomeles fruits belonged to six classes, namely fatty acids, alcohols, aldehydes, esters, ethers and alkanes. Aldehydes 7-hexadecenal and heptacosanal, and alkanes hexatriacontane and tetrapentacontane were the main constituents in the soluble cuticular waxes of C. speciosa and C. × superba fruits, accounting for more than half of the total contents. However, alkane tetrapentacontane, alcohol 8,10-hexadecadien-1-ol and heptacosanal prevailed in C. japonica fruit waxes. Isopropanolic fruit extracts exhibited dose-dependent antimicrobial activity against four Gram-negative bacteria, five Gram-positive bacteria and one fungal strain in the disc diffusion assay. In general, extracts from the Chaenomeles fruits demonstrated higher activity against Gram+ bacteria than Gram- strains. The strongest inhibiting activity was shown against Staphylococcus epidermidis (by the fruit extracts of C. × superba and C. speciosa), Micrococcus lysodeikticus and Candida albicans (both by C. × superba fruit extract). Results of the study confirmed accumulation of the bioactive compounds in the fruit waxes of different Chaenomeles species and antimicrobial ability of Chaenomeles fruits as well. These findings revealed the bioactive compounds in fruit cuticular waxes and suggested health-promoting properties of introduced Chaenomeles species.