Performance Of Japanese Quail Research Articles

Supplementation of Azolla (Azolla pinnata) meal on growth performance of Japanese quails (Coturnix small)

Supplementation of Azolla (Azolla pinnata) meal on growth performance of Japanese quails (Coturnix small)

Study the Effect of Cordyceps militaris Supplement in the Diet on Growth Performance of Japanese Quail

Study the Effect of Cordyceps militaris Supplement in the Diet on Growth Performance of Japanese Quail

Effect of Incorporating Soursop (Annona muricata) Leaves Powder on Reproductive Performance of Japanese Quail (Coturnix coturnix japonica)

Introduction: Soursop leaves are rich in various molecules, including total phenols, terpenes, and steroids, which possess a range of pharmacological properties that can be utilized in animal production to enhance both growth and reproduction of animals. The present study aimed to evaluate the effect of incorporating soursop (Annona muricata; A. muricata) leaves powder into feed on the reproductive performance of Japanese quail. Materials and methods: A total of 80 Japanese quails (64 females and 16 males) aged two weeks were randomly divided into four experimental groups, labeled T0, T1, T2, and T3, and received feed additives with 0 mg/kg body weight (bw), 250 mg/kg bw, 500 mg/kg bw, and 750 mg/kg bw of powdered soursop leaves, respectively. Additionally, water was provided ad libitum, and the quails' weights were measured every 7 days for a period of 75 days. At the end of the period, 12 female quails from each group were sacrificed after 24 hours fasting period. Blood was collected for hematological (Leukocyte, Erythrocyte, and Platelet indices) and serum biochemical (total serum cholesterol, total proteins, albumin, and globulin, Aspartate aminotransferase, Alanine aminotransferase, Urea, and Creatinine) analysis. The males were also sacrificed to evaluate the spermatozoa characteristics (mobility, concentration, and viability). Results: No statistical significant changes in growth characteristics or hematological parameters were observed. However, biochemical parameters increased significantly with the inclusion of Soursop (A. muricate) in quail feed, including increased total cholesterol, total protein, and globulin levels, and decreased malondialdehyde levels. This effect was most significant at dosage of 500 mg/kg bw. Serum levels of urea, Alanine aminotransferase, and Aspartate aminotransferase were not significantly affected by A. muricata whatever the concentration considered. A significant increase in fast progressive spermatozoa, along with a decrease in immotile spermatozoa, was observed with A. muricate at dosage of 500 mg/kg bw compared to the control. Sperm viability also increased significantly, particularly in live at dosage of 500 mg/kg bw. Significant increase was observed in fertility parameters, including increased fertility rate, hatchability rate of fertile eggs, total hatchability rate, and chick weight, along with decreased embryonic mortality at 500 mg/kg bw treatment compared to the control. Conclusion: In conclusion, the findings indicated that incorporating A. muricata leaf powder at 500 mg/kg bw into quail feed positively influences reproductive cells and boosts fertility growth promoters.

Combined Effects of Dichrostachys glomerata (phytobiotic) with Graded Levels of Probiotics on the Production Performance of Japanese Quails

Combined Effects of Dichrostachys glomerata (phytobiotic) with Graded Levels of Probiotics on the Production Performance of Japanese Quails

The influence of dietary supplementation with fermented agro-industrial residue of faba bean on Japanese quail performance, immunity, gut microbiota, blood chemistry, and antioxidant status

Antibiotic overuse in poultry feeds has disastrous implications; consequently, long-term alternatives must be developed. As a result, the current study aims to assess the impact of Aspergillus niger filtrate (ANF) high in organic acids grown on agro-industrial residue of faba bean (AIRFB) on quail diet, as well as their influence on bird productivity, digestion, carcass yield, blood chemistry, and intestinal microbiota. A total of 240 Japanese quails (aged 7 d) were used in this study, divided equally among 5 experimental groups with 48 quails each. Group 1 (G1) received a basal diet without any ANF, group 2 (G2) received a basal diet supplemented with 0.5 mL ANF/kg diet, group 3 (G3) received a basal diet supplemented with 1.0 mL ANF/kg diet, group 4 (G4) received a basal diet supplemented with 1.5 mL ANF/kg diet, and group 5 (G5) received a basal diet supplemented with 2 mL ANF/kg diet. The performance parameters were monitored at 1 to 3, 3 to 5, and 1 to 5 wk. Adding ANF increased body weight at 3 and 5 wk, as well as body weight gain at 1 to 3, 3 to 5, and 1 to 5 wk, compared to the control diet. The ANF fed quails had the highest feed conversion ratio compared to the control group. The addition of ANF to the quail diet had no effect on the weight of the carcass, gizzard, heart, liver, giblets, or dressing; however, it did lower triglycerides, low-density lipoprotein, and very low-density lipoprotein while increasing high-density lipoprotein levels. The quail groups that received ANF had enhanced immunological indices such as IgG, IgM, IgA, and lysozymes. It also increased the levels of superoxide dismutase and total antioxidant contents, as well as catalase, and digestive enzymes such as protease, amylase, and lipase. However, it lowered the blood MDA levels compared to control. It has been demonstrated that the total gut microbiota, Escherichia coli, total coliforms, and the population of Salmonella are all reduced in ANF-fed quails. Histological examination of ANF quails' liver and intestinal sections revealed normal hepatic parenchyma, typical leaf-like intestinal villi, and comparatively short and frequently free lumina. In conclusion, Japanese quail showed improvements in performance, digestive enzymes, antioxidant indices, immunity, and capacity to reduce intestinal pathogenic bacteria after consuming diet supplemented with ANF.

Effect of Pure Corn Oil on Growth Performance of Japanese Quail (Coturnix Coturnix Japonica)

In recent years, consecutive research has been conducted on the nutrient requirements of the quails to find an optimum and affordable legitimate growth promoter. Different types of vegetable oil have been tested so far. This study aimed to evaluate the effects of pure corn oil (PCO) on growth performance and live body weight gain in Japanese quails. A total of 80 four-day-old quail chicks were divided into two groups: control (20 checks) and experimental (60 checks). The experimental group was subdivided into three subgroups (G1, G2, and G3) of 20 chicks per group. All the control and experimental group chicks were fed a commercial grower quail diet in all three growth phases. Despite the commercial diet, the G1, G2, and G3 experimental groups were fed 1, 3, and 5% PCO, respectively. The values for live body weight did not significantly change after adding PCO to the quail’s diet. According to the study's findings, adding PCO to the commercial diet of Japanese quails in various amounts had no beneficial effects on feed consumption and growth rate. Additionally, it had an insignificant (p>0.05) detrimental impact on the growth performance of the quails compared to control groups. In conclusion, we can say that adding PCO to quail's commercial diet in the hot months of the year under Afghanistan's climate has no positive impact on growth and further slows down the rate of body weight gain.

Comparison of different papaya forms on the growth performance, immune response, antioxidative capacity, and caecal microbiota of Japanese Quails

Papaya is a sparsely branched tree and grows in many tropical and subtropical areas. Herein, this study compared the effects of papaya at 0.75 % as seeds meal (PSM), leaves meal (PLM), latex powder (PLP), or a mixture of them (PMIX) on the performances of Japanese quails. Two hundred unsexed Japanese quail (1st week-old) with an initial body weight of 21.08 ± 0.32 g were allocated into five experimental groups in cages with free access to water and test diets. Birds fed diets enriched with PSM, PLM, PLP, or PMIX for 55 weeks. The results showed that quail groups fed on papaya diets exhibited improved (P < 0.05) final weight, weight gain (WG), and feed conversion ratio (FCR) than the control. Dietary PLP or PMIX resulted in a remarkable WG enhancement, while PMIX reduced the FCR (P < 0.05). Activities of digestive enzymes (amylase, trypsin, and chymotrypsin) were enhanced (P < 0.05) with dietary papaya, especially in birds fed on PLP. Quails fed on PLM, PLP, or PMIX diets had reduced albumin/globulin ratio (A/G), triglycerides, total cholesterol, and low-density lipoprotein levels. At the same time, the level of high-density lipoprotein and the activity of aspartate aminotransferase increased (P < 0.001) by dietary PLP and PMIX. Moreover, higher total antioxidant capacity, superoxide dismutase, and lower malondialdehyde were recorded with papaya supplements than with the control. A similar trend was recorded for immunoglobulin G and M (IgG and IgM), phagocyte activity, and phagocyte index. Interestingly, low caecal harmful microbiota populations were observed in birds treated with papaya. In conclusion, the dietary inclusion of different papaya parts (leaves, seed, latex, and their mixture) can improve the productive performance, immunity, antioxidant indices, intestinal enzymes, and decline intestinal pathogens of growing Japanese quails.

Effects of prenatal dichromatic light exposure on hatching results and post-hatch performance of Japanese quail.

Present study evaluated the effect of lighted incubation on pre- and post-hatch performance of Japanese quail. In a completely randomized design, 1200 eggs were evenly divided into 4 treatments groups having six replicates (each tray was considered as replicate), 50 eggs each. Different dichromatic lights (Green + Red; GR, Green + Blue; GB, and Blue + Red; BR) of 250 lux were provided during incubation for 12 h daily and effects of these lights very evaluated on hatching results and post-hatch growth. After hatch, 600 quail chicks were divided into 4 treatments, 6 replicates, and 25 birds each. Regarding hatching traits, better hatchability was found in the GR group compared to GB, BR, and dark group; while early embryonic mortality was lower in BR, GB, and dark group than GR; mid embryonic mortality was lower in dark group and late embryonic mortality was noted in the GR group than those of other treatment groups. In addition, moisture loss during incubation was minimum in BR and dark groups; however, chick spread was better in the GR group. In terms of growth performance, weight gain was better in the GR group; feed intake in dark, feed conversion ratio in BR, and livability were better in BR and GR group. In morphometrics, keel and shank length were higher in all the colored groups (GB, BR, and GR) whereas body length, wing spread, shank circumference, drumstick length, and circumference were higher in the GR group. Regarding serum chemistry, glucose, albumin, and globulin levels were higher in the GR group. It was concluded that under the experimental conditions, GR light at the prenatal stage to Japanese quail eggs positively influenced hatching performance and post-hatch growth.

Effect of Spirulina and Probiotic on Growth Performance of Japanese Quail

The aim of current project is to fortify the quail feeds with various types of supplements such as spirulina, Used as a substitute for Prob. &amp; study the growth performance to recognize the best formula of spirulina for best production. untreated was feed on standard diet, Spirulina group was feed with Spirulina , Probiotics complex group was feed with probiotics complex, Mix Spirulina with probiotic complex group was feed with above Spirulina &amp; probiotic. complex. BW, BWG, FI &amp; FCE were daily recoded. Results noted at highest significant increase (P≤0.05) for the addition of spirulina occurred in the average body weight of Japanese quail compared to the control and other treated groups. The highest significant increase (P≤0.05) occurred in the BWG rates of birds treated with the addition of spirulina for weeks (1 and 4) and the cumulative (1-6) weeks. the highest rate (p≤0.05) of feed consumption was recorded in the group of birds treated with spirulina for weeks (2, 3, 4, 5, and 6). and the cumulative feed consumption rate from (1-6) weeks compared with the other treatments and the control group. Rresults showed FCE rates recorded a significant improvement in adding spirulina during (1,2,3,4,6) weeks and the cumulative feed conversion efficiency average (1-6) weeks for birds. From this research, we concluded that adding spirulina to quail diets improved productive traits.

Effects of black pepper and turmeric powder on growth performance, gut health, meat quality, and fatty acid profile of Japanese quail.

In poultry production, the search for alternatives to in-feed antibiotics continues unabated. This study investigated the effects of dietary supplementation of black pepper and turmeric powder, separately or in combination, on the growth performance, gastrointestinal microbiota population, intestinal morphology, serum biochemical parameters, meat quality, and meat fatty acid profile in Japanese quails. Five hundred-day-old mixed-sex Japanese quail chicks were randomly assigned to one of five treatments: a control diet (CON); CON +0.2% antibiotic flavomycin as an antibiotic growth promoter (AGP); CON +0.5% turmeric powder (TUP); CON +0.5% black pepper powder (BPP); and CON +0.5% TUP, and 0.5% BPP (MIX). The findings showed that quail chicks fed AGP and TUP throughout the rearing period had better body weight gain (p = 0.007) and feed conversion ratio (p = 0.02) than the other treatments. The TUP, BPP, and MIX feeds reduced (p = 0.005) abdominal fat percentage. The MIX group had a better breast muscle water-holding capacity (p = 0.04) and lightness index (p = 0.02) and lower (p = 0.02) malondialdehyde concentration after 7days of refrigerated storage. Feeding BPP, TUP, and MIX diets decreased (p = 0.001) serum cholesterol concentration. Quail chicks fed the CON diet showed significantly higher coliform counts in the crop and ileum (p < 0.001), whereas the lactic acid bacterial population was lower (p = 0.008) in the ileum. Birds that received the MIX diet exhibited a higher (p = 0.02) villus height to crypt depth ratio in the duodenum compared to the other groups. The tested feed additives increased (p < 0.001) villus height in the jejunum and ileum compared to other groups. Feeding the TUP, BPP, and MIX diets reduced (p < 0.001) total saturated fatty acid content and increased (p = 0.004) total polyunsaturated fatty acid concentration, where the MIX diet had the best results. Overall, the present data indicate that supplementing the basal diet with turmeric powder enhances the growth performance of Japanese quails. In some respects, such as gut health and meat quality, combining turmeric powder and black pepper powder was more effective than using them independently.

Effect of Egg Weight and Type of Breeding on the Productive Performance of Japanese Quail

This study was conducted in the farm of the Department of Animal Production of the College of Agriculture - Tikrit University for the period from 8/11/2021 to 2/2/2022, and the aim of the study was the effect of egg weight and breeding system on the productive performance of Japanese quail during the growth and egg production stages. 490 un naturalized one-day-old birds were raised from egg hatching collected and graded into three weight categories, low (8-10) gm, medium (10.1-11) gm and high (11.51-12.5) gm and divided into Three groups based on the weight of hatched eggs from (high, medium, low) to a ground breeding system and cages. The study included two stages, the first is the growth stage from (1-36) days old, and the second is the production stage from(37-78) days old. The results of the statistical analysis showed the following: (1) Effect of the breeding system: (A) There is a significant difference (P≤0.05) for quails raised in the ground breeding system in the following characteristics: weight at sexual maturity, while there were no significant differences in age at sexual maturity in the growth stage. (B) There is a significant (P≤0.05) superiority (P≤0.05) of quail birds raised by the ground breeding system in the following characteristics: average egg weight. While there were no significant differences in the weight of eggs for the first, second and third periods, the number of eggs. (2) Effect of egg weight, (A) There is a significant superiority (P≤0.05) of hatching quail birds from high-weight eggs in the characteristics of weight at sexual maturity, and age at sexual maturity for the stage of growth. Whereas, significant superiority (P≤0.05) was found in the following traits during the production stage: (A) High weight eggs: weight of the first egg, weight of eggs. (B) Low weight eggs: there were no significant differences between the treatments in terms of number of eggs,

Feeding Effect of Organic and Inorganic Zinc with Vitamin E on Growth Performance and Carcass Quality of Japanese Quail

The research facility of Avian Research &amp; Training (ART) Center (Poultry Production), UVAS, Lahore was selected to investigate the effect of feeding organic and inorganic zinc in addition to vit-E on the growth performance of Japanese quail. for a period of 6 months. The diet formulated by the inclusion of ZnO + ZnI (mg)+VE(IU) as 0+0+0 (A), 25+0+0 (B), 0+15+0 (C), 0+0+12 (D), 25+15+0 (E), 25+0+12 (F), 0+15+12 (G) and 25+15+12 (H), respectively. The effect of zinc and vit-E on growth and slaughter traits of Japanese quail was significant (P&lt;0.05) and it was perceived that in 6 weeks the broiler quails in groups H and F took higher feed (745.44 and 734.70 g/bird), with simultaneous effect on LBW of males (175.35 and 174.50 g), females (198.35 and 197.38 g), average (186.85 and 185.94 g), intestine filled (6.81 and 6.66 g) and empty weights (5.51 and 4.88 g), intestine length (44.87 and 44.75 g), cecal length (16.37 and 16.12 cm), the weight of carcass + giblets (142.01 and 141.48 g), carcass (130.88 and 130.25 g), heart (2.18 and 2.16 g), liver (5.02 and 5.06 g), gizzard (5.62 and 5.21 g), respectively. The dietary Zn + vit-E inclusion significantly (P&lt;0.05) increased the relative length of gut segments, villi height, villus thickness, the villi height to crypt depth proportion in jejunum and ileum and the number of goblet cells in various parts of the intestine of quails. The meat production was significantly higher in female quails than the meat of male quails. This suggested that the organic Zn proved to be better than the inorganic Zn source in addition to vit-E to achieve physiology-related traits in quails.

Sweet Potato Peel as Substitute Dietary Energy Source on the Early Laying Performance, Egg Quality Traits and Yolk Biochemical Composition of Japanese Quails (Coturnix coturnix japonica Temminck &amp; Schlegel, 1898)

This study evaluated the potential of sweet potato peels from different varieties as substitute dietary energy in the early laying performance of Japanese quails. A total of 240 heads of ready-to-lay quails were laid out in a Randomized Complete Block Design (RCBD) and were assigned to the following treatments; ACommercial feeds, B-Home-mixed ration with 10% NISC Sp-35, C- Home-mixed ration with 10% NISC Sp-36, and D- Home-mixed ration with 10% Sp Japonita. The data were analyzed using one-way ANOVA for RCBD, and differences were determined using Tukey's Honestly Significant Difference Test (HSD) at p&lt;0.05. The commercial diet improved hen-day egg production, FCR per dozen eggs, FCR per kilogram egg mass, and eggshell thickness. On the other hand, there were no significant differences (p&gt;0.05) observed in egg weight, egg shape index, dry shell weight, yolk-albumen ratio and yolk total antioxidant activity. The yolk color score of HMR diets was significantly higher (p&gt;0.05) and the yolk cholesterol deposition was lesser than those in the commercial diet. The financial analysis showed that the gross return was highest in the commercial ration. However, HMR diets performed better in terms of net income, return over feed cost, egg-to-feed price ratio, benefit-cost ratio, and return over investment.

Effect of using Guava and olive waste with or without enzyme addition in the diets on growing Japanese quail performance

Effect of using Guava and olive waste with or without enzyme addition in the diets on growing Japanese quail performance

Effect of feed particle size on growth and egg-laying parameters of Japanese quail (Coturnix japonica) in Côte d’Ivoire

In Côte d’Ivoire, the quail breeding is confronted with the lack of food specific to the species. Thus, in the south-east of this country, an experimental study was conducted at the ISMOREL farm in the sub-prefecture of Adiaké. This study, which aims to improve the productivity of day-to-day farms, focused on the grain size of the feed. From grower (GD) and layer (LD) diets, 5 experimental diets have been manufactured. There are: LD 5 with a particle size of 5 mm, LD 3 and GD 3 with a particle size of 3 mm as well as LD 2 and GD 2 with a particle size of 2 mm. The growth parameters related to 750 two-week-old unsexed subjects weighing 49.67 ± 0.17 g on average. For egg-laying, 300 female quails were used at 6-week-old. The results showed an influence of the 2 mm grain size (LD 2 and GD 2) on parameters such as feed intake (186.96 ± 4.73 g and 153.46 ± 5.33 g), average live weight (142.37 ± 1.76 g and 155.85 ± 1.85 g), the average daily gain (4.29 ± 1.22 g/d and 4.74 ± 1.25 g/d), the age of first egg laid (49.33 ± 0.58 d and 46.33 ± 1.26 d) and egg-laying rate (67.24 ± 30.97% and 74.12 ± 34.03%). At the end of this study, it appears that the 2 mm particle size induces better growth and egg-laying performance in Japanese quail.

Growth and egg production performance of Japanese quails (Coturnix coturnix japonica) fed diets containing graded levels of ginger waste meal (Zingiber officinale) as a partial replacement for maize

Growth and egg production performance of Japanese quails (Coturnix coturnix japonica) fed diets containing graded levels of ginger waste meal (Zingiber officinale) as a partial replacement for maize

Effect of three different processing techniques of soybean on nutritional and growth performance of Japanese quail (Coturnix japonica)

The experiment investigated the effects of various soya bean groups (boiled, fermented, and roasted) on Japanese quail at 3 weeks old. 160 Japanese quail were randomly assigned to four treatments (control, boiling soya beans, fermented soya beans, roasted soya beans) with four duplicates each. The 12-week trial lasted. Data on weekly body weights and feed conversion ratio were analyzed using analysis of variance (ANOVA) and Tukey's honestly significant at 5% probability test. The result shows there are significant differences in weekly weights of Japanese quail at weeks 1(828.12-1083.24g), 2(1026.47-1362.02g), and 3(1325.69-1528.20g) with the highest observed in birds in treatment 2(boiled soya beans). The maximum FCR was in week 1 for all treatments, while the lowest was in treatment 3 for weeks 5 and 9 (0.83; P&lt;0.005). Week 1 to week 12 feed conversion ratio decreases. The birds' feed conversion ratios varied significantly (P&lt;0.05). The quails in treatment 4 (roasted soybeans) had the greatest weekly weight after the trial (1742.34g). Thus, quails in treatment 3 (roasted soybean) had the best development performance than the control, boiled and fermented. So it advised that roasted soybean can be an efficient diet for Japanese quails for maximum performance.

Effect of seeds, oil, and black seed meal on Japanese Quail's productive performance

.The research aims to effect using seeds, oil and black seeds meal on the productive performance of Japanese Quail. Seven experimental diets were used in the study; T1 was the control (with 0% seeds, oil or black seed meal), and T2 and T3 contained black seeds (BC) at two levels, 0.80 and 1.60%, respectively. T4 and T5 black seed oil (BSO) was added at 0.50 and 1% levels, respectively, while the last two treatments, T6 and T7, contained black seed meal (BSM) at two levels of 7 and 14%, respectively. Mash feed and water were submitted ad libitum during the whole period. The study includes one stage; the growth period (1-35 days old). This research was conducted at a poultry farm, Animal Production Department, College of Agriculture and Forestry, University of Mosul, Mosul, Iraq. A total of 588 desert color Japanese quail (Coturnix coturnix japonica), unsexed at one day old, were randomly distributed in seven previous treatments of six replicates with 14 birds in each in the growth period. The results showed significant effect (p≤ 0.05) in body weight(g/bird),body weight gain(g/bird), Protein digestion coefficient(%), Preslaughter weight(g/bird), carcass weight(g/bird), for T3. While the data showed a significant effect (p≤ 0.05) for T2 in water intake. The data confirmed the superiority of the T3, T5 and T7 in carbohydrate digestion coefficient(%). The growth rate recorded a significant effect (p≤ 0.05) for T3, T4, T5 and T6. As well as, T3 reported a low significant effect (p≤ 0.05) on feed conversion ratio(g feed: g WG) and water conversion ratio(ml: g WG). The best net revenue(Iraqi Dinar: g WG ) was T7 and T3. No significant differences were founded in all other traits in this study. Keys words: seeds, oil, black seeds meal-productive performance -Japanese Quail.

Effect of Dietary Incorporation of Shatavari (Asparagus racemosus) on Production Performance of Japanese quail

The present research was conducted to evaluate the effect of incorporation of Shatavari (Asparagus racemosus) root powder (SRP)in the diet on the performance and carcass yield of Japanese quail. 150-day old quail chicks were randomly allotted to 5 dietary treatment groups with 3 replicates of 10 birds for 7 wk. The SRP was ground and included at 0% (Ts0; Control), 1.0 (Ts1), 1.5 (Ts2), 2.0 (Ts3) and 2.5% (Ts4) levels in isocaloric and iso-nitrogenous quailchick’s diets to meet the nutrient requirements as per NRC (1994).There was a significant increase (P&lt;0.05) in BWG, overall feed intake and improved (P&lt;0.05) FCR with incorporation of SRP at 2.5% in the diet compared to the control group. There was a significant difference (P&lt;0.05) in the carcass traits with respect to dressing wt, eviscerated wt, edible wt and giblet wt(i.e. liver, heart and gizzard). However, incorporation of SRP at 2.5% level had higher carcass yield (P&lt;0.05) compared to the control group. Thus, it is concluded that SRP can be safely incorporated at 2.5% level in quail diets without any adverse effects.

Impact of spraying eggs with betaine after exposure to short-term thermal stress during early embryogenesis on pre and post-hatch performance of Japanese quail

It is essential to understand and manage environmental factors for good quail production and welfare. One of the most important environmental stressors that hinder quail productivity is heat stress. This study aimed to evaluate the impact of spraying Japanese quail (Coturnix coturnix japonica) eggs with betaine after exposure to short-term high temperature during early embryogenesis on pre and post-hatch performance of quail. A total of 750 eggs were equally divided into two groups. Eggs in the first group were incubated at normal incubation temperature (37.5 °C/NIT), while those in the second group were incubated at high incubation temperature (39.0 °C/HIT) for 3 h daily from day 4–6 of incubation. Eggs in both groups were subjected to five treatments, NC (negative control), PC sprayed distilled water (positive control), while B0.5, B1, and B2 treatments were sprayed with distilled water supplemented with 500, 1000, and 2000 mg betaine/L, respectively. The chick weight at hatch, slaughter weight, and first egg weight was significantly impaired by the HIT treatment. The HIT group revealed a significant increase in cloacal temperature, H/L ratio, liver enzymes, triglyceride, and cholesterol and a significant decrease in hatchability, T3 hormone, and blood protein levels than the NIT group. Regarding betaine effects, the embryonic mortality rates, hatchability, hatched chick weight, and oviduct percentage in groups treated with 1000 or 2000 mg betaine/L were significantly improved compared with the control. Also, spraying betaine at 1000 or 2000 mg/L significantly increased blood protein and triiodothyronine (T3) hormone levels and significantly decrease liver enzyme levels and total feed consumption compared with the untreated group. The right/total ventricle ratio (RV/TV) of quail in HIT group was significantly increased, while betaine treatment significantly decreased this ratio. Considering these results, it is strongly suggested that spraying of betaine on eggs at 2000 mg/L optimizes Japanese quail performance.