Japanese Burkitt's Lymphoma Research Articles

A Case of Japanese Burkitt's Lymphoma Associated with EB Virus

A 6-year-old girl was admitted for cervical lymphadenopathy. She was diagnosed histologically as having Burkitt's lymphoma with partial involvement of follicles. Immunological study of a biopsy specimen demonstrated that many cells were surface immunoglobulin (lambda light chain)-positive, and chromosomal analysis revealed a translocation of (8;14) (q24;q32) in all blastic cells. Her serum antibody titers to Epstein-Barr virus (EBV) capsid antigen and to early antigen were 1:320 and 1:160, respectively, and most of the cells from the lymphoma biopsy specimen were EBV-associated nuclear antigen (EBNA)-positive. In addition, EBV DNA/DNA reassociation kinetic analysis showed 22 EBV genome equivalents per cell. Complete remission was obtained by CHOP therapy (consisting of cyclophosphamide, adriamycin, vincristine, and prednisolone) combined with cervical irradiation. She is in complete remission now (17 months after the onset).

Japanese Burkitt's Lymphoma: Clinicopathological Review of 14 Cases

The clinicopathological features of 14 Japanese patients with Burkitt's lymphoma (seven studied by us and seven reported by others) are reviewed. Five patients were 12 years of age or younger. Six patients presented with abdominal masses and five with jaw tumors. Twelve patients manifested blood and/or marrow involvement at the time of initial diagnosis (four patients) or later in the course of the disease (eight patients). Most of the patients had a rapid clinical course in spite of combination chemotherapy. The malignant cells from all patients exhibited a B-cell phenotype; the surface immunoglobulin was IgM in 13 and IgG in one. Two patients had tumors positive for Epstein-Barr virus-determined nuclear antigen while all the others lacked this antigen in their tumors. Chromosome analysis revealed a standard t(8;14) translocation in nine patients, a 14q+ chromosome without an identifiable donor chromosome in two and variant translocations t(2;8) in two and t(8;22) in one.

Chromosome translocations, surface immunoglobulins, and Epstein-Barr virus in Japanese Burkitt's lymphoma

Chromosome translocations, surface immunoglobulins, and Epstein-Barr virus in Japanese Burkitt's lymphoma

Burkitt's lymphoma with [formula omitted] translocation: A aase report with special reference to the clinical features

A case of Japanese Burkitt's lymphoma (BL) with a t(2; 8)(p11; q24), a variant translocation in BL, is reported. The patient was a 45-year-old woman in whom a subcutaneous right axillar tumor first occurred. Remission was not achieved despite extensive chemotherapy. Of the four nonendemic BL, two endemic BL, and one nonendemic Burkitt-type acute lymphocytic leukemia (ALL-L3) cases with a t(2; 8) reported so far, including the present case, four (two nonendemic and two endemic) were positive for Epstein—Barr virus-determined nuclear antigen (EBNA) and two revealed extremely high antibody titers against Epstein—Barr virus (EBV), the remaining one not having been tested for EBV. Thus, a possible close relationship between the t(2; 8) and EBV infection has to be considered. The t(2; 8) in nonendemic BL seems to occur more often in adults than in children.

Chromosome abnormalities in Japanese Burkitt lymphoma cell lines.

Six established Japanese Burkitt lymphoma (BL) cell lines including one case with null cell type were studied by chromosomal banding techniques. The modal chromosome number was diploid or nearly diploid in five cases and hyperdiploid in one case. The marker chromosome 14q+ was observed in four of the six cases; the origin of the extra band was a chromosome 8 in three including the null cell case but could not be identified in the other. The two cases lacking the 14q+ marker had variant translocations involving the long arm of chromosome 8, one of which carried a translocation, t(8;22) (q24;q13) and the other a translocation, t(2;8) (p12;q24). Although structural and/or numerical aberrations were found in all six cell lines, chromosome 8 was the one most consistently involved. This frequent involvement of chromosome 8 in aberrations; therefore, may be an important event in the development of BL rather than the presence of a 14q+ marker chromosome.

8-14 translocation in a Japanese Burkitt's lymphoma.

Chromosome analysis was performed on cells obtained from the pleural effusion of a Japanese patient with Burkitt's lymphoma. Two modal chromosomal numbers were found: 45 and 46. Five different karyotypes were present, all having a t (8q-;14q+) translocation. This case illustrates that Burkitt's lymphomas of Japanese are no exception to the frequent association of this chromosomal abnormality with Burkitt's lymphomas.

Studies on a Japanese Burkitt Lymphoma

Studies on a Japanese Burkitt Lymphoma

Studies on a Japanese Burkitt Lymphoma

Studies on a Japanese Burkitt Lymphoma

Establishment of an Epstein-Barr virus-negative B-cell lymphoma line from a Japanese Burkitt's lymphoma and its serial passage in hamsters

An Epstein-Barr virus (EBV)-negative lymphoma line (JBL) was established in vitro from pleural effusion of an EBV-seropositive 29-year-old Japanese female with Burkitt's lymphoma. JBL cells as well as her original lymphoma cells bore monoclonal surface IgM with lambda light chains. The JBL line grew in single cell suspension with a doubling time of 30 hours. Attempts were made to serially transplant JBL cells in antilymphocyte serum-treated newborn hamsters; intraperitoneal implantation of 1-3 X 10(7) cells gave rise to invasive tumors in all recipients with death after 10 to 14 days. The hamster-passage line, now in the 9th passage, has been converted to an ascitic form with progression to leukemia in some animals. A "starry sky" pattern closely resembling the human tumor material was preserved in every tumor through serial animal passage.