Italian-type Salami Research Articles

Preparation, physicochemical and microbiological evaluations of italian type salami fermented with kefir

Industrialized meat product, known as salami, is composed of some types of meat, such as pork, beef or pork and beef together, added ingredients and bacon. This research aimed to elaborate Italian salami using the fermentation process with standard starter bacteria, such as control, water and milk kefir, and evaluate the physicochemical and microbiological characteristics of the final product. The use of kefir grains aimed to analyze the possibility for their use in the production of salami as starter cultures from their symbiotic association of yeasts, acid-lactic bacteria, acetic bacteria and also their probiotic and antimicrobial action. The microbiological and physicochemical composition indicates that kefir is a product with probiotic characteristics, because it has in its composition living microorganisms capable of improving the intestinal microbial balance producing beneficial effects on the health of the individual who consumes it. At the end of the analyses, it was observed that the salami based on standard starter bacteria culture presented greater diversity of fungi, and the fungus Exophiala spp was present in the 3 types of salamis. The samples showed absence of pathogenic bacteria such as Salmonella ssp, Shigella ssp and E.coli. There was little variation in the physical-chemical characteristics of the salamis evaluated. It was concluded that kefir can replace industrial fermentation culture without affecting the microbiological and physicochemical pattern of salami.

Avaliação da qualidade sensorial, instrumental e microbiológica de salames tipo Italiano, adicionados de culturas starters

Objetivou-se com esse estudo, produzir salames com e sem a adição de culturas starters e avaliar o efeito das mesmas sobre as características sensoriais, físicas e microbiológicas dos produtos. Foram elaborados 3 tratamentos de salames tipo italiano: T1 (controle - sem adição de cultura starter); T2, produto com adição de culturas starters Lyocarni RHM-33 (Pediococcus pentosaceus e Staphylococcus xylosus) e T3 com adição de culturas starters Lyocarni SBM-11 (Staphylococcus xylosus, Staphylococcus carnosus, e Lactobacillus sakei). A contagem de bactérias láticas nos produtos inoculados ao final do 21º dia de processamento, foi de 2,9 x 106 UFC/g, e 3,2 x 105 UFC/g, para os tratamentos 2 e 3 respectivamente. A contagem de coliformes totais e termotolerantes não foi afetada (P>0,05) pela interação entre tratamentos. A presença de culturas starters nos tratamentos não afetou os índices de luminosidade (L*) e Índice de amarelo (b*) em relação ao tratamento controle (p>0,05). Para índice de vermelho e saturação, o Tratamento 2 apresentou maiores valores (P<0,05). Apenas os parâmetros de dureza e mastigabilidade foram afetados (p<0,05) pela adição das culturas starters. Os dados obtidos com a análise sensorial mostraram que o tratamento T2, apresentou-se como o melhor aceito entre os salames produzidos, para os atributos de sabor, textura e impressão global. Com isso, este trabalho mostrou que devem ser estudados profundamente os aspectos físicos, microbiológicos e sensoriais para a escolha de culturas starters, pois a interação dos microrganismos interferem nos aspectos tecnológicos dos produtos.

Applicability of potentially probiotic Lactobacillus casei in low-fat Italian type salami with added fructooligosaccharides: in vitro screening and technological evaluation

The aim was to evaluate the use of Lactobacillus casei strains in the fermentation process of low-fat Italian type salami with fructooligosaccharides (FOS). A screening using probiotic strains was performed at pH 5.5, 5.0 and 4.5 and incubation temperatures of 15 and 25 °C. Lactobacillus casei SJRP66 and Lactobacillus casei SJRP169 were selected and added to the low-fat fermented sausage – C (control), FOS (25% reduced fat with 2% FOS), FOS_66 (25% reduced fat with 2% FOS and L.casei SJRP 66) and FOS_169 (25% reduced fat with 2% FOS and L.casei SJRP 169). The evaluation included pH, moisture, lactic acid bacteria count, probiotic count, weight loss, instrumental color, TBARS and texture parameters. FOS_66 and FOS_169 presented a good probiotic count (8 log CFU/g) and similar technological behavior to the control. The addition of the probiotic showed no effect on lipid oxidation and * value. These strains of probiotic showed promising properties for applications in low-fat Italian type salami with healthier appeal.

Evaluation of celery extract (Apium greveolens L.) as a natural curing agent in the production of Italian-type Salami with native starter cultures

The aim of this study was to evaluate celery extract as a natural curing agent in the production and characterization of Italian-type Salami with native starter cultures SxLp (S. xylosus AD1 and L. plantarum AJ2). Two formulations were produced for Italian-type Salami, to one of which was added 1.4% celery extract (T1) and to the other 0.3% commercial curing salt (T2). Maintaining the same conditions of temperature, relative humidity and air velocity, the salamis were matured for 26 days, and physicochemical, microbiological and sensorial analyses were performed. Starter culture SxLp produced a reduction in pH, promoting greater acidification of salami and reduction of nitrate, allowing color formation in the product. Moreover, the cultures remained viable (> 7 log CFU.g-1), in both treatments, during the 26 days of maturation. The combination of native starter culture with celery extract promoted microbiological safety, adequate texture and the characteristic color of cured meat sausage. Salami T1, containing starter cultures SxLp and celery extract, was well accepted by the judges, most of whom would often buy the product. Thus, the application of celery extract as a curing agent together with the addition of the native starter cultures, proved to be viable for production of Italian-type salami.

EFFECTS OF NATURAL ANTIOXIDANTS IN PROCESSING AND STABILITY OF ITALIAN TYPE SALAMI DURING STORAGE

The aim of the study was to elaborate formulations of Italian type salami by adding natural antioxidant of green tea (Camellia sinensis) and to evaluate the stability during the shelf life. Formulations (F1, F2 and F3) were elaborated varying the concentrations of green tea (0.008 to 0.016%), sodium erythorbate (0.048 to 0.097%), sodium nitrite (0 to 0.012%), sodium nitrate (0 to 0.016%), curing salt (0 to 0.189%) and dehydrated glucose (0.589 to 0.959%), keeping fixed the concentrations of swine meat, bacon, water, refined salt, pepper and starter culture. To verify oxidative stability, the moisture, water activity, sodium nitrate and nitrite, pH, acidity, TBARS and product acceptance were evaluated during 120 days of storage. F1 stood out in terms of acceptance, with a higher initial and residual concentration of nitrates and nitrites up to the 80 th day of storage and less lipid oxidation, that has 0.016% green tea, 0.012% sodium nitrite, 0.013% sodium nitrate, 0.081% sodium erythorbate and 0.806% dehydrated glucose in its formulation. The results indicated that the addition of green tea, associated with sodium nitrate/nitrite and sodium erythorbate reduced the formation of TBARS and did not affect the sensory quality of the product. Thus, this natural antioxidant can be easily used in Salami to improve quality and provide safer products.

Can dietary fiber improve the technological characteristics and sensory acceptance of low-fat Italian type salami?

To meet the needs of new consumers, meat researchers need to develop healthier products. Dietary fibers can be added for structural purposes, to present functional characteristics or to change the composition of the final product. In this study, mixture design was used to investigate the effects of partial substitution of pork fat by inulin, fructooligosaccharides and α-cyclodextrin on the technological and sensory quality characteristics of low-fat Italian type salami. The partial substitution of fat using dietary fibers shows no effect on weight loss, Aw and pH during ripening time. However, the addition of up to 2% α-cyclodextrin increased lightness and reduced redness and yellowness. Up to 2% of inulin or fructooligosaccharides added improved the sensory acceptance, texture parameters and redness. Healthier low-fat Italian type salami can be produced using inulin or fructooligosaccharides as fat substitute for pork fat and still obtain good technological and sensorial results.

Características físicas e sensoriais de Salame Tipo Italiano com adição de própolis

No presente estudo foram elaboradas quatro formulações de salame tipo italiano, sendo uma isenta de antioxidante (controle), uma adicionada de antioxidante sintético (BHT) e duas contendo extrato de própolis (0,01% e 0,05%). As amostras foram submetidas a análises microbiológicas, sensoriais e perfil de textura e cor, além da perda de peso e o pH durante o período de maturação. Os resultados mostraram perda de peso das formulações durante a maturação, indicando que a incorporação da própolis não afetou o processo de secagem. A formulação adicionada de 0,01% de própolis apresentou coloração vermelha mais acentuada no final da maturação em comparação as demais. Os valores de pH variaram entre algumas amostras, bem como em relação ao dia de maturação. A adição de BHT acelerou o processo de desidratação do salame, deixando-o com maior grau de dureza. As formulações controle e aquela adicionada de 0,05% de própolis apresentaram melhores resultados para o perfil de textura, embora não tenham diferido das demais em relação ao atributo textura durante o teste sensorial. A formulação controle e a contendo BHT apresentaram os melhores resultados para intenção de compra e preferência. Por outro lado, todas as formulações apresentaram percentuais de certeza de compra acima de 70%, sugerindo aceitação por parte dos consumidores.

Ochratoxin A production by Aspergillus westerdijkiae in Italian-type salami

The occurrence of ochratoxin A (OTA) in matured meat products can be attributed to mycotoxin coming from raw materials and/or molds that develop on the product surface during ripening. This work aimed to evaluate OTA production by Aspergillus westerdijkiae inoculated on the surface of sausages and its diffusion into the product throughout ripening, study the effect of relative humidity (RH) on the production of this mycotoxin, and investigate the presence of OTA in dry fermented sausages that naturally present intense contamination by A. westerdijkiae. Italian-type sausages were surface inoculated with A. westerdijkiae and two distinct experiments were performed. In the first, the sausages were matured in a chamber with decreasing RH (from 95 to 75%) for 35 days. In the other, the sausages were incubated under different RH (79, 85 or 95%) for 21 days. Samples were taken at the beginning of the experiments and every 7 days, subdividing into casing, outer border, and core for analyses. Sausage samples naturally spoiled by ochratoxigenic fungi were collected during sanitary inspection. Even in the presence of A. westerdijkiae mycelia, no OTA was detected for up to 7 days of sausage maturation. On the other hand, this study demonstrated that the growth of A. westerdijkiae on salami surface produces high amounts of OTA on the casing and allows its diffusion through the casing with contamination to the outer border of sausages. In the same way, it shows that under similar water activity values of substrate, RH influences the amount of OTA produced. Conversely, OTA was restricted to the casing in the naturally contaminated sausages.

Application and evaluation of propolis, the natural antioxidant in Italian-type salami

Summary This study aimed to characterize propolis with respect to its antioxidant activity and apply it to the elaboration of Italian-type salami. A propolis sample was collected and subjected to chemical and physicochemical characterization and its antioxidant capacity determined. Four salami formulations were developed: F1 (no antioxidants); F2 (addition of 0.01% BHT); F3 (addition of 0.01% propolis) and F4 (addition of 0.05% propolis). The salamis were evaluated with respect to their physicochemical properties and lipid oxidation. The characterization of the propolis showed a high level of waxes and low levels of phenolic compounds and flavonoids, although in sufficient quantity to prove their antioxidant activity. The Italian-type salamis showed moisture, protein and lipid contents which conformed to the limits preconized by Brazilian legislation. The F4 formulation (0.05% propolis) showed a better result when compared to the formulations F3 (0.01% propolis) and F1 (no antioxidant). However, formulation F2 (0.01% BHT) showed the lowest value of lipid oxidation. The results showed that propolis inhibits oxidative action and can be added to meat products as a natural antioxidant.

Chemical composition, microbiological properties, and fatty acid profile of Italian-type salami with pork backfat substituted by emulsified canola oil

ABSTRACT: Vegetable oils have been used to substitute pork backfat to improve the fatty acid profile of fermented sausages. The aim of this study was to assess the chemical composition, microbiological properties, and fatty acid profile of Italian-type salami with pork backfat substituted (15% and 30%) by emulsified canola oil. Fat contents decreased while moisture contents increased in Italian-type salami with emulsified canola oil. The growth of lactic acid bacteria in salami was not affected by canola oil and absence of fecal coliforms, coagulase-positive staphylococci, and Salmonella were reported during processing of fermented sausages. Lower levels of saturated fatty acids (SAFAs), higher levels of monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) were observed in salami with emulsified canola oil. Together, our results indicated that substituting pork backfat with emulsified canola oil improved the nutritional values of Italian-type salami.

Evaluation of Biogenic Amines Levels, and Biochemical and Microbiological Characterization of Italian-type Salami Sold in Rio de Janeiro, Brazil.

The objective of the present study was to evaluate the levels of biogenic amines (cadaverine, putrescine, tyramine, histamine, spermidine and spermine) by high performance liquid chromatography (HPLC) and the physicochemical (moisture, lipids, proteins, pH, water activity and fixed mineral residue) and microbiological (lactic acid bacteria and aerobic heterotrophic mesophilic bacteria count) characteristics of six Italian-type salami brands sold in the city of Niteroi (Rio de Janeiro, Brazil). The salami showed lactic acid bacteria count from 5.7 to 8.6 CFU•mL-1, and heterotrophic mesophilic bacteria count from 5.8 to 8.7 CFU•mL-1. Three brands showed moisture contents above 35% and one brand had protein content below 25%. The mean values obtained for the amines were: 197.43, 143.29, 73.02, 4.52, 90.66 and 36.17 mg•kg-1 for tyramine, putrescine, cadaverine, spermidine, histamine, and spermine respectively. Two brands presented histamine contents above the legal limit established in 100 mg•kg-1. We concluded that the evaluated salami presented a wide variation in the count of the bacterial groups with a predominance of lactic acid bacteria. The moisture contents indicate insufficient drying before commercialization and protein content had values below the minimum limit determined by the Brazilian legislation. Finally, the levels of biogenic amines found could cause adverse reactions in susceptible consumers, depending of the amount and frequency of intake of these products.

Characterization of a two-peptide plantaricin produced by Lactobacillus plantarum MBSa4 isolated from Brazilian salami

The aim of this study was to explore the biochemical and genetic features of the two-peptide bacteriocin produced by a Lactobacillus plantarum strain isolated from Italian type salami produced in Brazil (Lb. plantarum MBSa4). Identification of bacteriocinogenic Lb. plantarum MBSa4 was performed by 16S rRNA sequencing. Expressed bacteriocin was evaluated for spectrum of activity, heat and pH stability, mechanism of action, and molecular mass. Partial purification was achieved by cation-exchange, and reversed phase - HPLC. Total DNA of Lb. plantarum MBSa4 was extracted and tested for presence of previously described bacteriocin genes. Bacteriocin MBSa4 was heat-stable, unaffected by pH 2.0 to 6.0 and active against all tested Listeria monocytogenes strains and most of tested fungi. Maximal production (1600 AU/ml) in MRS broth occurred after 22 h at 25 °C, presenting bacteriostatic activity as result of combined action of two components. The molecular mass determined by SDS-PAGE was 2.3 kDa. PCR-amplified DNA indicated the same nucleotide sequence of plantaricin W. Results indicate that Lb. plantarum MBSa4 produces plantaricin W, a two-peptide lantibiotic with remarkable anti-Listeria activity.

Características físico-químicas e aceitação sensorial de salame tipo Italiano com adição de óleo de canola

The aim of this study was to evaluate the effects of partial pork fat replacement with emulsified canola oil in Italian-type salami. Three treatments were done: Control (100% pork fat, without fat replacement), T1 (15% pork fat was replaced by emulsified canola oil) and T2 (30% pork fat was replaced by emulsified oil canola). There were evaluated the salamis’ physicochemical characteristics (pH, water activity, weight loss, color and lipid oxidation) during the manufacture and storage period, and sensory evaluation after the manufacture process. The emulsified canola oil addition at different levels did not change the pH and color during the manufacture process, even though significant differences were found in these parameters during the storage period. The water activity did not differ significantly among the treatments. However, the treatments with emulsified canola oil added have a lower weight loss than the control. It was possible to observe an elevation on lipid oxidation values in the T2 during manufacture and storage periods, while in the T1, the values did not differ from the control at the end of manufactures and remained lower than the control during the storage period. Even more, the partial replacement of pork fat by emulsified canola oil did not affect the acceptance of the product for aroma, flavor, color, texture and visual appearance. Thus, the 15% pork fat replacement by emulsified canola oil in Italian-type salami is a viable alternative for the product diversification.

Comparative Analysis and Validation Methodologies of GC and HPLC for Analysis of Cholesterol in Meat Products

This study validated different extraction methodologies and compared the quantification of cholesterol by gas chromatography (GC) and high performance liquid chromatography (HPLC) in mg per 100 g of Italian-type salami and traditional bologna. The GC method used was direct saponification of the samples without derivatizations and the HPLC method was used to extract of the lipid samples. The GC limits of detection and quantification obtained for cholesterol were, respectively, 0.001 and 0.003 mg.g–1. The HPLC values were 0.005 mg.g–1 and 0.016 mg.g–1. The GC recovery rate was 97.10 ± 0.13 and that of HPLC was 93.33 ± 0.22. Comparison of the cholesterol quantity found using the two chromatographic techniques shows that both are capable of quantifying cholesterol in the foods. With regard to costs, analysis time, the cost/benefit relationship was better with gas chromatography than that obtained with high performance liquid chromatography.

Análise físico-química de salames coloniais comercializados no município de Toledo, Estado do Paraná

Italian immigration in the west of Paraná State contributed for the development of small meat industries. The colonial Italian-type salami is one of the main commercialized meat products in this region, therefore making necessary its quality evaluation. The aim of this work is to verify whether the composition of the colonial salami is in accordance with Brazilian law, and the nutritional facts information on the labels of these products. Four different marks of colonial Italian-type salami produced in the region of Toledo, Paraná State and one great trade mark of Italian-type salami were herein evaluated. Properties like moisture, percentages of proteins, ashes, lipids and cholesterol were evaluated. We recorded a great discrepancy between values presented in the nutritional facts information and determined values, except for the industrialized salami. The moisture content was higher than the maximum levels recommended by the legislation. On the other hand, protein contents were lower than the minimum values recommended, and only the fat content matched the values established by Brazilian law. The cholesterol contents were lower than described on the labels when they contain them. According to the composition of the product and the Brazilian legislation, such salami could not be classified as Italian-type salami.

Lipid and protein oxidation in the internal part of italian type salami containing basil essential oil (Ocimum basilicum L.)

Different concentrations of basil essential oil (Ocimum basilicum L.) (0.19; 0.38; 0.75; 1.87; 3.75 and 6.00 mg.g-1) were evaluated in relation to their antioxidant activity using the DPPH● radical methodology. From the IC50 obtained data, the concentrations of 0.19; 0.38; 0.75; 1.87; 3.75; 6.00 and 12.00 mg.mL-1 were applied directly to the product and these were sensorially evaluated by the test of control difference. The concentrations related to the highest acceptability (0.19; 0.38 and 0.75 mg.g-1) were tested for antioxidant activity in the internal part of Italian type salami - during the processing and after 30 days of storage, in terms of lipid and protein oxidation. The oxidation of lipids was determined using the method of TBARS. The method of carbonyl compounds was employed for proteins oxidation. Five different formulations of salami were elaborated: blank (without the use of antioxidant); control (using sodium eritorbate as antioxidant); and adding 0.19; 0.38 and 0.75 mg.g-1 of basil essential oil. The product was kept between 25 oC and 18 oC and UR between 95% and 70%, for 28 days. Analyses were carried out on the processing day and after 2, 7, 14, 21 and 28 days, and also following 30 days of storage. The basil essential oil in vitro presented an antioxidant activity of IC50 12 mg.mL-1. In the internal part of the Italian type salami the commercial antioxidant (control) and the formulation containing 0.75 mg.g-1 of basil essential oil presented antioxidant activity in relation to the lipids, but not to the proteins - during processing and storage.

Reduction of Drying and Ripening Times During the Italian Type Salami Production

Reduction of Drying and Ripening Times During the Italian Type Salami Production

Cholesterol oxide, cholesterol, total lipid and fatty acid contents in processed meat products during storage

The effects of storage time on the formation of cholesterol oxides and on alterations in the fatty acid composition of processed meat products manufactured by Brazilian industries were investigated in this study. Cholesterol oxides and cholesterol were determined by HPLC using photodiode array and refractive index detectors. Samples of jerked beef, Italian-type salami, chicken mortadella and Chester mortadella were analysed at 30 day intervals starting at zero time, for 90 days for the mortadella and 120 days for the jerked beef and salami. The mortadellas were stored under refrigeration at 6 °C and the jerked beef and salami at room temperature, but protected from the light. No cholesterol oxides were formed during the storage time in any of the samples. The cholesterol content, the fatty acid composition and total lipid contents showed no significant differences during storage with the exception of the total lipid content of the jerked beef, which varied from 3.5 at zero time to 2.4 g/100 g after 120 days storage.

Atividade antimicrobiana de bactérias lácticas isoladas de produtos cárneos frente a Listeria monocytogenes e Staphylococcus aureus

De seis amostras de pernil desossado curado e três amostras de salame tipo italiano artesanal foram isoladas 288 cepas de bactérias lácticas. Pelo teste de inibição direta 59 delas foram capazes de inibir in vitro o desenvolvimento de cepas de Listeria monocytogenes e de Staphylococcus aureus, o que demonstra que produzem compostos inibitórios.

Production of salami from ostrich meat with strains of Lactobacillus sake, Lactobacillus curvatus and Micrococcus sp.

The aim of this study was to produce Italian-type salami from ostrich meat using different combinations of Lactobacillus sake, Lactobacillus curvatus and Micrococcus sp., and to compare the sensory characteristics of these products to that of salami produced with glucono-delta-lactone (GdL). Meat inoculated with starter cultures was fermented for four days (20–22 °C, 97–99% RH) and ripened for a further 11 days (16–18 °C, 40–60% RH). Cell counts of lactic acid bacteria and micrococci, and changes in pH were determined daily during fermentation. According to texture and sensory evaluation, the best salami was produced by a starter culture containing L. curvatus DF 38 and Micrococcus sp. MC 50.