Isoxsuprine Hydrochloride Research Articles

Development of a New Isoxsuprine Hydrochloride-Based Hydroxylated Compound with Potent Antioxidant and Anti-Inflammatory Activities.

The scientific community actively pursuits novel compounds with biological activities. In this context, our study utilized the predicted data mining approach (PDMA), which can efficiently screen out biotransformable precursor candidates to produce new bioactive compounds. The PDMA was applied to Bacillus megaterium tyrosinase (BmTYR) to form new bioactive hydroxyl compounds from isoxsuprine hydrochloride (isoxsuprine). The results show that isoxsuprine could be biotransformed by BmTYR to form a new compound, 3''-hydroxyisoxsuprine. 3''-Hydroxyisoxsuprine exhibited 40- fold and 10-fold higher potent antioxidant and anti-inflammation activities than the precursor, isoxsuprine. The 3''-hydroxyisoxsuprine effectively mitigates the hyperimmune response in RAW 264.7 macrophages by inhibiting the upregulation of pro-inflammatory cytokine (IL-1β and IL-6) and inflammatory enzyme COX-2 gene expression triggered by LPS stimulation. This study illustrates that PDMA is an effective strategy for screening known natural and chemical compounds and for generating new bioactive compounds through biotransformation. Our newly produced compound has potential future applications in pharmacology and biotechnology.

Preterm Labor with Side Effects: Compare the Effectiveness of Magnesium Sulfate (MgSO4) with Isoxsuprine

Background: In India, 25% of pregnancies develop preterm labor (PTL), resulting in 10 to 69% cases of preterm birth. Medical intervention to stop labor, reduce infection rate, and avoid infant respiratory distress has been the subject of studies for a long time. PTL patients usually get tocolytics, corticosteroids, antibiotics, and other clinically symptomatic and supportive therapy to accomplish this goal. Studies further showed that these tocolytic drugs lower intracellular calcium bioavailability via biochemical pathways, hindering the interaction of actin-myosin. Due to the poor success rate of labor arrest, researchers concluded from their studies that widespread adoption of medical management for PTL has been hampered. The high rate of major side effects of tocolytic drugs, particularly beta-mimetic ones, exacerbated this. We know of no clinical evidence on PTL management in India. Objective: The effectiveness and maternal side effects of MgSO4 and isoxsuprine in PTL arrest. Methods: In our study, we included a total of 82 pregnant women who had PTL discomfort and were admitted to the labor department. Both groups were randomly assigned patients. “Group 1 patients received isoxsuprine hydrochloride, whereas group 2 patients received MgSO4”. After that, a comprehensive clinical examination included vital signs, general, systemic, external genitalia, and PV (per vaginal) results. Investigations include CBC, BT, CT, urine full examination, ABO, RH group, serum electrolytes, RBS, vaginal swab, and Renal function test (RFT). Result: Significant difference (p <0.05) indicated that MgSO4 was more effective. Conclusion: MgSO4 can be used as a tocolytic agent in PTL as it shows better tolerance capacity when compared to isoxsuprine.

Vaginal Drain to Prevent Ascending Infection in Preterm Premature Rupture of Membranes: A Novel Method.

Not to delay delivery for more than 24 hours after rupture of the membrane is the thumb rule in obstetrics. Ascending infection from the vagina results in dangerous chorioamnionitis, which threatens the lives of the baby and mother. We developed a novel method to prevent ascending infection by using a vaginal drain, which helps to continue pregnancies if the leak stops and buys some time for antenatal steroids to act if the leak doesn't stop. In this study, 20 uncomplicated singleton pregnant women with spontaneous preterm premature rupture of membranes at gestational ages <35 weeks were recruited. Under the speculum, vaginal epithelial debris and secretions were cleared by a saline wash. The tip of the Nelaton Catheter was kept in the posterior fornix and then strapped to the thigh. The outer end was connected to the collection bag system and allowed to hang down to the ground. The foot end of the cot was raised. Leaked amniotic fluid and amniotic fluid index (AFI) were measured daily. A daily AFOD estimation was done for leaked AF. Uterine activity was controlled with low-dose Isoxsuprine Hydrochloride rapid infusion tocolysis. Antenatal steroids (ANS) were given. The spread of infection was monitored by maternal pulse rate, fetal heart rate, TC, and CRP. Pregnancies were terminated when leaks didn't stop, AFI didn't raise, or mature AFOD was observed. The number of women in whom leaks stopped, the number of dayspregnancies continued, neonatal respiratory distress (NRD), birth weights, and perinatal deaths were recorded. Results: Leaks stopped in eight (40%) women. Pregnancy continuation ranged from 7 to 74 days. In 12 women, the leak did not stop, but we could buy 2-5 days' time for ANS to act. All parameters of the infection were within normal limits. Thirteen babies developed mild NRD, and we lost one baby. By using a vaginal drain for P(P)ROM, ascending infection can be prevented, leaks can be stopped in 40% of women, and can buy time for ANS to act.

EFFICACY OF ISOXSUPRINE AS AN ADJUVANT IN MANAGEMENT OF OSMF: A RANDOMIZED CLINICAL TRIAL

Introduction: Oral submucous brosis is a persistent, insidious mucosal condition that is primarily brought on by areca nut use. It can also be brought on by excessive use of peppers, dietary deciencies, autoimmune, genetics, and environmental factors. The aim of the Aim Of The Study: study was to evaluate the effectiveness of vasodilator isoxsuprine as an adjuvant in the oral submucous brosis management. Study Design: Twenty patients with OSMF were recruited and randomly two groups were divided. Group A: Patients got treated with Isoxsuprine hydrochloride 10 mg thrice daily and antioxidants (Lycopene) twice daily for 3 months. Whereas, in the Group B, antioxidants two times a day for 3 months were given. Both were instructed for mouth opening exercises. All individuals, mouth opening, burning sensation were recorded and reassessment was done at every 15 days during the treatment and after 3 months follow-up and followed by statistical analysis. Visual Analogue Scale Results: (VAS) score for severity of burning sensation was observed in both the groups A &amp;B was statistically signicant whereas interincisal opening was higher in group A patients. Isoxsuprine Hydrochloride can be suggested as an adjunctive t Conclusion: herapy in oral submucous brosis management.

Preterm Labour Management with Glyceryl Trinitrate Patch and Isoxsuprine: A Longitudinal Study

Introduction: Nitric oxide donors in the form of nitroglycerine and β-mimetic drugs (Isoxsuprine hydrochloride) are commonly used in managing preterm labour. It is one of the most challenging obstetric complications encountered by obstetricians, as well as preterm neonates for paediatricians. Preterm labour refers to the onset of uterine contractions of sufficient strength and frequency to effect progressive dilatation and effacement of the cervix between 20 and 37 weeks of gestation. Aim: To compare the effects of transdermal Glyceryl Trinitrate (GTN) with Isoxsuprine in terms of maternal complications, perinatal outcomes, and their tocolytic effectiveness. Materials and Methods: A hospital-based longitudinal study was conducted in the Department of Obstetrics and Gynaecology at JNU Medical College and Research Centre, Jaipur, Rajasthan, India. The study duration of the study was one year, from March 2022 to February 2023. A total number of 100 cases of preterm labour were enrolled and equally divided into two groups: Groups A (GTN patch) and group B (Isoxsuprine) with a defined dosing schedule. Singleton pregnancies with intact membranes and gestational age of 26-36 weeks were included in the study, metting the diagnostic criteria for preterm labour. Both the groups received pharmacological intervention, and outcome measures such as success rate of treatment (i.e., prolongation of labour),. maternal side effects, and foetal outcomes {Neonatal Intensive Care Unit (NICU) admission and Appearance, Pulse, Grimace, Activity, and Respiration (APGAR) score) were measured and evaluated. Predefined parameters and other data were tabulated in Microsoft Excel and statistical analysis was performed using Statistical Package for Social Sciences (SPSS) version 20.0. The Chi-square test was applied to identify significant differences. Results: The incidence of preterm labour was highest in the age group of 21-30 years (76% in Group A and 70% in Group B) among patients treated with nitroglycerine and isoxsuprine, respectively. The mean prolongation of pregnancy in the nitroglycerine group was 21.42 days, and in the isoxsuprine group, it was 16.22 days (p=0.042). The most common side effect in the nitroglycerine group was headache (60%), while in the isoxsuprine group, it was maternal hypotension (24%). The mean APGAR score in Group A and Group B was 8.18 and 8.06, respectively. It was observed that 24% of newborns in the nitroglycerine group and 36% in the isoxsuprine group required NICU admission. The success rate was 90% in the nitroglycerine group and 80% in the isoxsuprine group. Conclusion: Both drugs were effective in prolonging pregnancy for the first 48 hours, but nitroglycerine was found to be slightly better in terms of success rate. Nitroglycerin was more acceptable to patients with preterm labour, as it did not have to be administered through painful injections.

Sensitive Cloud Point Extraction Method for the Determination of Isoxsuprine Hydrochloride in Pharmaceutical forms using Spectrophotometry

A simple and highly sensitive cloud point extraction process was suggested for preconcentration of micrograms amount of isoxsuprine hydrochloride (ISX) in pure and pharmaceutical samples. After diazotization coupling of ISX with diazotized sulfadimidine in alkaline medium, the azo-dye product quantitatively extracted into the Triton X-114 rich phase, dissolved in ethanol and determined spectrophotometrically at 490 nm. The suggested reaction was studied with and without extraction and simple comparison between the batch and CPE methods was achieved. Analytical variables including concentrations of reagent, Triton X-114 and base, incubated temperature, and time were carefully studied. Under the selected optimum conditions, the linearity ranges of calibration curves were 1-9 and 0.5-8 µg/mL with detection limits of 0.26 and 0.09 µg/mL of ISX for batch and CPE methods respectively. A relative standard deviation (RSD %) best than 1.98 and 2.67 % with the percentage recoveries range 100.14 and 99.63 % were obtained for both methods respectively. The proposed methods were successfully used in routine analysis of ISX in pharmaceutical forms with high accuracy and reproducibility.

Effects of tocolytic drugs (isoxsuprine hydrochloride) during the implantation period in pregnant sheep.

In this study, the effects of isoxsuprine hydrochloride applied 14 and 15 days after insemination in Anatolian Merino Sheep on lamb yield and some blood parameters were investigated. The research was conducted during the breeding season and 54 ewes inseminated on the same day were used. The ewes were assigned to three groups. Group I: For the placebo effect, physiological saline was injected on the 14th day into half of the control group and on the 15th day into the other half after insemination (n=18). Group II: Tocolytic drug was injected on the 14th day after insemination (n=18). Grop III: Tocolytic drug was injected on the 15th day after insemination (n=18). As the tocolytic drug, isoxsuprine hydrochloride (HCl) 3 ml (Utelax, Sanovel, Türki̇ye) was used intramuscularly. The number of pregnant and viviparous ewes, single and multiple birth ewes, lambs per viviparous ewes were determined as reproductive parameters. Blood progesterone, cortisol and calcium concentration were determined. As a result, it was determined that the single application of isoxsuprine hydrochloride (3 ml) in the implantation period did not have a positive or negative effect on reproductive parameters, and did not change the blood progesterone, calcium and cortisol concentration in ewes.

Solid Phase Extraction-Spectrophotometric Determination Of Isoxsuprine Hydrochloride In Different Samples Using Modified Magnetic Nanoparticales

A green, simple and sensitive spectrophotometric method was proposed for the determination of isoxsuprine hydrochloride (ISX), based on the preconcentration with cetyltrimethylammonium bromide (CTAB) modified magnetic iron oxide nanoparticales (MNPs). ISX was coupled with diazotized metoclopramide drug via diazotization-coupling reaction in alkaline medium to form yellow-orange color product which was extracted and measured spectrophotometrically at λmax444 nm. Various parameters affecting the azo-dye removal by adsorption on modified magnetic nanoparticales (Fe3O4@CTAB) such as pH of solution, volume of diazotized reagent, volume of surfactant, amount of adsorbent and type of desorbing solvent were studied. The linear calibration graph were obtained in the concentration range of 0.3-7.0 µg/mL of ISX, the limit of detection and the limit of quantification calculated to be 0.29and 0.99µg/mL, respectively. Accuracy and precision described by relative standard deviation and relative recovery percentages. The results obtained show that the CTAB-coated MNPs could be employed in the extraction of the drug in the azo dye form from different samples. The ISX was extracted successfully in pharmaceutical forms and spiked samples of river water.

Multidimensional analysis for corrosion inhibition by Isoxsuprine on mild steel in acidic environment: Experimental and computational approach

This study investigates the impact of Isoxsuprine hydrochloride drug on mild steel corrosion in a 1.0 M HCl solution by employing weight loss, electrochemical methods, surface morphological techniques, and finally validated with theoretical studies like quantum calculation and MD simulation. Inhibitory efficacy of Isoxsuprine hydrochloride came out to be ∼ 97 % at 2000 ppm concentration. The analysis demonstrated the increase in the inhibition efficiency with the rising concentration of inhibitor and decreasing effectiveness with increased temperature. Thermodynamic parameters were also investigated by utilizing the data obtained from temperature. Potentiodynamic polarization validated that the Isoxsuprine hydrochloride retarded the cathodic as well as anodic reactions. During the adsorption process, the Isoxsuprine hydrochloride molecules followed the Langmuir adsorption isotherm. Theoretical calculations like quantum chemical calculation and molecular dynamics simulation also confirms excellent adsorption of Isoxsuprine hydrochloride on the metallic sample. The results from all of the different methods were consistent.

Spectrophotometric Determination of Salbutamol Sulphate and Isoxsuprine Hydrochloride in Pharmaceutical Formulations

A simple, sensitive and accurate spectrophotometric method has been developed for the determination of salbutamol sulphate (SAB) and isoxsuprine hydrochloride (ISX) in pure and pharmaceutical dosage. The method involved oxidation of (SAB) and (ISX) with a known excess of N-bromosuccinamid in acidic medium, and subsequent occupation of unreacted oxidant in decolorization of Evans blue dye (EB). This, in the presence of SAB or ISX was rectilinear over the ranges 1.0-12.0, 1.0-11.0 µg/mL, with molar absorptivity 4.21×104 and 2.58×104 l.mol-1.cm-1 respectively. The developed method had been successfully applied for the determination of the studied drugs in their pharmaceutical dosage resulting in a good agreement with certified value and standard addition procedure.

Different greenness assessment perspectives for stability-indicating RP-HPLC method used for the assay of isoxsuprine hydrochloride and four nephrotoxic and hepatotoxic photothermal degradation products

Nowadays, the analytical community focuses on applying principles of green analytical chemistry (GAC) to develop new analytical methods to reduce harmful effects on the environment and human beings. In this work, we developed, validated, and assessed the greenness profile of a RP-HPLC method for stability and purity assay of isoxsuprine hydrochloride (ISX) and four of its nephrotoxic/hepatotoxic photothermal degradation products; p-hydroxyacetophenone (AC), 1-methyl-3-phenylpropylamine (AM), and p-hydroxybenzaldehyde (HB) in pure form, laboratory-prepared mixtures, and in the pharmaceutical dosage form. A C18 Waters XTerra (3.5 μm, 4.6 × 100 mm) separated the five analytes using a mobile phase composed of 20 mM potassium dihydrogen phosphate (adjusted to pH 2.3 with ortho-phosphoric acid) and methanol. We performed gradient elution at a constant flow rate of 0.8 mL/min while monitoring the effluent absorbance at 216 nm. The method shows high sensitivity with limits of quantifications equal to 0.557, 0.304, 5.791, 0.446, and 0.497 μg/mL for ISX, AC, AM, BZ, and HB, moreover, ISX low limit of detection (0.184 µg/mL) enabled its detection in spiked human plasma at its therapeutic level of concentration (0.4 µg/mL). We used four greenness assessment tools to assess the method greenness, including the “National Environmental Method Index” (NEMI), the “Eco-Scale Assessment” (ESA), the “Green Analytical Procedure Index” (GAPI), and Analytical Greenness metric (AGREE); additionally, we used the tools as a comparative greenness study between the proposed and the reported HPLC methods. The comparison proved that the proposed method was the best regarding the principles and guidelines of GAC. Our method proved superior eco-friendly qualitative and quantitative scores relative to the reported methods, where the method achieved more green NEMI and GAPI quadrants, scored the higher ESA score (85) than reported (81,81, and 77), and the higher AGREE score (0.71) than reported (0.53,0.56,0.58).

Catalytic activity and structural changes of catalase in the presence of Levothyroxine and Isoxsuprine hydrochloride

Two drugs that pregnant women (with hypothyroidism) may use during pregnancy include Isoxsuprine hydrochloride (ISO) and levothyroxine (LEV); ISO to reduce uterine contractions and LEV for the treatment of hypothyroidism. In the current work, we explored the mechanism of binding affinity between the above drugs and antioxidant enzyme Bovine Liver Catalase (BLC). The experimental results confirmed that both drugs could bind with BLC to form drug−BLC complexes but LEV showed a higher binding affinity toward enzyme. The binding constants of LEV–and ISO–BLC were 0.42 × 105 and 0.13 × 104 M−1 at 310 K, respectively. LEV enhanced the catalase activity but the enzymatic activity of BLC reduced gradually in the presence of ISO. Both drugs were able to induce conformational changes in the BLC structure. The results of the molecular docking investigations confirmed the experimental data and showed that the main binding forces in the LEV−BLC and ISO–BLC systems were hydrogen bond and hydrophobic force. The best binding site of both drugs on BLC is located at a cavity among the wrapping domain, N-Terminal arm, and β-barrel.

Computational optimization of a novel solid-state sensor for stable assay of isoxsuprine hydrochloride in the presence of its nephrotoxic/hepatotoxic photothermal degradation products: application in different sampling matrices

The DOE optimization of a solid-state potentiometric sensor for isoxsuprine assay in the presence of its photothermal degradation products within complex matrices.

A Validated Simple Titrimetric Method for the Quantitative determination of Isoxsuprine hydrochloride from Pharmaceutical dosages

A simple precise, rapid accurate and sensitive titration method was developed for quantitative determination of isoxsuprine hydrochloride in pharmaceutical dosage form. The titration was carried out using standardized 0.1 N sodium hydroxide solution. The proposed method was found to be precise with % RSD 0.99) between 10% to 50% of 500 mg of drug, substance weight. The percentage recovery of isoxsuprine hydrochloride in the optimized method was 99.02% to 99.84%. The method is also found to be rugged when checked by different analysts and using different lots of reagents and different laboratories.

A Validated Potentiometric titration method for Quantitative Determination of Isoxsuprine hydrochloride from Pharmaceutical Preparation

A Validated Potentiometric titration method for Quantitative Determination of Isoxsuprine hydrochloride from Pharmaceutical Preparation

Effectiveness and Safety of Isoxsuprine Hydrochloride as Tocolytic Agent in Arresting Active/Threatened Preterm Labor and Its Role in Maintenance Tocolysis: A Prospective, Open-Label Study.

The aim of the study is to obtain insights on the short and long-term safety and effectiveness of isoxsuprine hydrochloride as a tocolytic agent in the management of PTL. In this prospective, single-center, noncomparative study, patients (with preterm labor at gestational age of 24-37 weeks) were administered intravenous (IV) infusion of 40-mg isoxsuprine hydrochloride until uterine quiescence, followed by intramuscular (IM) injection of isoxsuprine hydrochloride 10 mg/4-hourly for first 24 hours and maintained with retard 40-mg sustained release capsule (two times a day) till the time of delivery or 37 completed weeks of pregnancy. All patients (n = 50) achieved successful tocolysis in 24 hours and 48 hours postadministration of isoxsuprine hydrochloride (IV/IM/oral). Mean (±SD) gestation age at the time of delivery was 39.8 ± 2.1 weeks, with latency period of 58.5 ± 18.7 days. Pregnancy outcomes were normal in all the patients and no congenital anomaly/fetal infection was reported. Mean (±SD) fetal birth weight was 2.7 ± 0.3 kg; mean (±SD) Apgar score at 1 and 5 minutes were 7.5 ± 0.6 and 9.2 ± 0.4, respectively. Maternal tachycardia and vomiting (8.0% each) were the commonly reported adverse drug reactions, which were resolved with dose adjustment. Isoxsuprine was found to be an effective and well-tolerated tocolytic agent in arresting PTL, in turn resulting in the overall improvement in maternal and perinatal outcomes.

Binding interaction of isoxsuprine hydrochloride and levothyroxine to milk β-lactoglobulin; from the perspective of comparison

Isoxsuprine hydrochloride (ISO) and levothyroxine (LEV) are medicines which can be utilized alone or simultaneously by pregnant women. The purpose of this work is to investigate the separate and simultaneous interaction of ISO and LEV with β-LG. The results showed that both drugs can bind to β-LG; the static quenching was suggested for fluorescence quenching mechanism of β-LG.The values of binding constants (Kβ-LG-ISO = 2.69 × 104 M−1, Kβ-LG-LEV = 0.54 × 103 M−1 and Kβ-LG–ISO–LEV = 2.18 × 103 M−1 at 310 K) suggested that ISO has stronger binding affinity toward β-LG than LEV and affinity of β-LG to LEV is increased in the presence of ISO while the presence of LEV has no significant effect on the affinity of protein to ISO. Thermodynamic parameters showed that the binding of LEV to β-LG are hydrogen bonding and Van der Waals forces but the formation of β-LG–ISO is hydrophobic associations. The results of FT-IR and UV–visible measurements indicated that the binding of both drugs to β-LG may induce conformational changes of protein. In silico molecular docking analyses confirmed that ISO and LEV binds to residues located at site I and site II of β-LG, respectively.

Novel kinetic spectrophotometric method for estimation of certain biologically active phenolic sympathomimetic drugs in their bulk powders and different pharmaceutical formulations

A simple, selective and sensitive kinetic spectrophotometric method was described for estimation of four phenolic sympathomimetic drugs namely; terbutaline sulfate, fenoterol hydrobromide, isoxsuprine hydrochloride and etilefrine hydrochloride. This method is depended on the oxidation of the phenolic drugs with Folin-Ciocalteu reagent in presence of sodium carbonate. The rate of color development at 747–760nm was measured spectrophotometrically. The experimental parameters controlling the color development were fully studied and optimized. The reaction mechanism for color development was proposed. The calibration graphs for both the initial rate and fixed time methods were constructed, where linear correlations were found in the general concentration ranges of 3.65×10−6–2.19×10−5molL−1 and 2–24.0μgmL−1 with correlation coefficients in the following range 0.9992–0.9999, 0.9991–0.9998 respectively. The limits of detection and quantitation for the initial rate and fixed time methods were found to be in general concentration range 0.109–0.273, 0.363–0.910 and 0.210–0.483, 0.700–1.611μgmL−1 respectively. The developed method was validated according to ICH and USP 30 –NF 25 guidelines. The suggested method was successfully implemented to the estimation of these drugs in their commercial pharmaceutical formulations and the recovery percentages obtained were ranged from 97.63%±1.37 to 100.17%±0.95 and 97.29%±0.74 to 100.14±0.81 for initial rate and fixed time methods respectively. The data obtained from the analysis of dosage forms were compared with those obtained by reported methods. Statistical analysis of these results indicated no significant variation in the accuracy and precision of both the proposed and reported methods.

Spectrophotometric and spectrofluorimetric methods for determination of certain biologically active phenolic drugs in their bulk powders and different pharmaceutical formulations

Two simple and sensitive spectrophotometric and spectrofluorimetric methods for the determination of terbutaline sulfate, fenoterol hydrobromide, etilefrine hydrochloride, isoxsuprine hydrochloride, ethamsylate, doxycycline hyclate have been developed. Both methods were based on the oxidation of the cited drugs with cerium (IV) in acid medium. The spectrophotometric method was based on measurement of the absorbance difference (ΔA), which represents the excess cerium (IV), at 317nm for each drug. On the other hand, the spectrofluorimetric method was based on measurement of the fluorescent of the produced cerium (III) at emission wavelength 354nm (λexcitation=255nm) for the concentrations studied for each drug. For both methods, the variables affecting the reactions were carefully investigated and the conditions were optimized. Linear relationships were found between either ΔA or the fluorescent of the produced cerium (III) values and the concentration of the studied drugs in a general concentration range of 2.0–24.0μgmL−1, 20.0–24.0ngmL−1 with good correlation coefficients in the following range 0.9990–0.9999, 0.9990–0.9993 for spectrophotometric and spectrofluorimetric methods respectively. The limits of detection and quantitation of spectrophotometric method were found in general concentration range 0.190–0.787 and 0.634–2.624μgmL−1respectively. For spectrofluorimetric method, the limits of detection and quantitation were found in general concentration range 4.77–9.52 and 15.91–31.74ngmL−1 respectively. The stoichiometry of the reaction was determined, and the reactions pathways were postulated. The analytical performance of the methods, in terms of accuracy and precision, were statistically validated and the results obtained were satisfactory. The methods have been successfully applied to the determination of the cited drugs in their commercial pharmaceutical formulations. Statistical comparison of the results with the reference methods showed excellent agreement and proved that no significant difference in the accuracy and precision.

Tocólisis con sulfato de magnesio o clorhidrato de isoxuprina en amenaza de parto pretérmino

ObjectiveTo compare the efficacy of intravenous magnesium sulphate with isoxsuprine hydrochloride in the treatment of threatened pre-term labour. Materials and methodsA total of 82 patients with a gestational age between 24 and 34 weeks and threatened pre-term labour were selected to receive intravenous magnesium sulphate (GroupA) or intravenous isoxsuprine hydrochloride (GroupB). An analysis was performed on the time of ceasing of uterine contractions and maternal adverse effects. ResultsTocolysis was successful in the first 24hours in 61.0% of patients in GroupA and 61.0% in patients of GroupB (P=ns). After 7days of treatment, 36.6% of patients in GroupA and 36.6% of patients in GroupB still were without contractions (P=ns). A delay in delivery over 34weeks was obtained in 19.5% of patients treated with magnesium sulphate and 26.8% in patients treated with isoxsuprine hydrochloride (P=ns). Nausea, tremor, and vomiting were more frequent in GroupA, while tremors and sweating were more frequent in GroupB (P=ns). ConclusionMagnesium sulphate is as effective as isoxsuprine hydrochloride for tocolysis in threatened pre-term labour.