Relative Isolation Research Articles

Segatella clades adopt distinct roles within a single individual’s gut

Segatella is a prevalent genus within individuals’ gut microbiomes worldwide, especially in non-Western populations. Although metagenomic assembly and genome isolation have shed light on its genetic diversity, the lack of available isolates from this genus has resulted in a limited understanding of how members’ genetic diversity translates into phenotypic diversity. Within the confines of a single gut microbiome, we have isolated 63 strains from diverse lineages of Segatella. We performed comparative analyses that exposed differences in cellular morphologies, preferences in polysaccharide utilization, yield of short-chain fatty acids, and antibiotic resistance across isolates. We further show that exposure to Segatella isolates either evokes strong or muted transcriptional responses in human intestinal epithelial cells. Our study exposes large phenotypic differences within related Segatella isolates, extending this to host-microbe interactions.

Characterisation of Neisseria gonorrhoeae strain differences in patients with multisite infection

BackgroundNeisseria gonorrhoeae, the aetiological agent of gonorrhoea, is an increasing global health priority due to high levels of antimicrobial resistance (AMR). It is estimated that up to 42% of patients...

Candida albicans isolates contain frequent heterozygous structural variants and transposable elements within genes and centromeres.

The human fungal pathogen Candida albicans poses a significant burden on global health, causing high rates of mortality and antifungal drug resistance. C. albicans is a heterozygous diploid organism that reproduces asexually. Structural variants (SVs) are an important source of genomic rearrangement, particularly in species that lack sexual recombination. To comprehensively investigate SVs across clinical isolates of C. albicans, we conducted long read sequencing and genome-wide SV analysis in three distantly related clinical isolates. Our work included a new, comprehensive analysis of transposable element (TE) composition, location and diversity. SVs and TEs are frequently close to coding sequences and many SVs are heterozygous, suggesting that SVs might impact gene and allele-specific expression. Most SVs are uniquely present in only one clinical isolate, indicating that SVs represent a significant source of intra-species genetic variation. We identified multiple, distinct SVs at the centromeres of Chromosome 4 and Chromosome 5, including inversions and transposon polymorphisms. These two chromosomes are often aneuploid in drug resistant clinical isolates, and can form isochromosome structures with breakpoints near the centromere. Further screening of 100 clinical isolates confirmed the widespread presence of centromeric SVs in C. albicans, often appearing in a heterozygous state, indicating that SVs are contributing to centromere evolution in C. albicans Together, these findings highlight that SVs and TEs are common across diverse clinical isolates of C. albicans and that the centromeres of this organism are important sites of genome rearrangement.

Epidemiological and clinical features of a large blastomycosis outbreak at a paper mill in Michigan.

Blastomycosis is an environmentally acquired fungal infection that can result in severe pulmonary illness and high hospitalization rates. In 2023, a blastomycosis outbreak was detected among workers at a paper mill in Delta County, Michigan. We included patients with clinical and laboratory evidence of blastomycosis who had spent ≥40 hours in Delta County since September 1, 2022 and had illness onset December 1, 2022-July 1, 2023. We assessed epidemiological and clinical features of patients and evaluated factors associated with hospitalization. We performed whole-genome sequencing to characterize genetic relatedness of clinical isolates from eight patients. In total, 131 patients were identified; all had worked at or visited the mill. Sixteen patients (12%) were hospitalized; one died. Compared with non-hospitalized patients, more hospitalized patients had diabetes (p=0.03) and urine antigen titers above the lower limit of quantification (p<0.001). Hospitalized patients were also more likely to have had ≥1 healthcare visits before receiving a blastomycosis diagnostic test (p=0.02) and to have been treated with antibiotics prior to antifungal prescription (p=0.001). All sequenced isolates were identified as Blastomyces gilchristii and clustered into a distinct outbreak cluster. This was the largest documented blastomycosis outbreak in the United States. Epidemiologic evidence indicated exposures occurred at or near the mill, and genomic findings suggested a common exposure source. Patients with diabetes may have increased risk for hospitalization, and elevated urine antigen titers could indicate greater disease severity. Early suspicion of blastomycosis may prompt earlier diagnosis and treatment, potentially reducing unnecessary antibiotic prescriptions and improving patient outcomes.

Integrative genomic, virulence, and transcriptomic analysis of emergent Streptococcus dysgalactiae subspecies equisimilis (SDSE) emm type stG62647 isolates causing human infections.

This study integrated genomic sequencing, mouse virulence assays, and bacterial transcriptomic analysis to advance our understanding of the molecular mechanisms contributing to Streptococcus dysgalactiae subsp. equisimilis emm type stG62647 pathogenesis. We tested a large cohort of genetically closely related stG62647 isolates for virulence using an established mouse model of necrotizing myositis and discovered a broad spectrum of virulence phenotypes, with near-mortality rates ranging from 20% to 95%. This variation was unexpected, given their close genetic proximity. Transcriptome analysis of stG62647 isolates responsible for the lowest and highest near-mortality rates suggested that these isolates used multiple molecular pathways to alter their virulence. In addition, some genes encoding transcriptional regulators and putative virulence factors likely contribute to SDSE emm type stG62647 pathogenesis. These data underscore the complexity of pathogen-host interactions in an emerging SDSE clonal group.

Paleoasian Substrate in the Gene Pool of Koryaks According to Data on Autosomal SNP Polymorphism and Y-Chromosome Haplogroups

The gene pool of the Koryaks was studied in comparison with other Far Eastern and Siberian peoples using a genome-wide panel of autosomal single-nucleotide polymorphic markers and Y-chromosome markers. The results of analyzing the frequencies of autosomal SNPs using various methods, the similarity in the composition of Y-chromosome haplogroups and YSTR haplotypes indicate that the gene pool of the Koryaks is as close as possible to the Chukchi one and was formed as a result of the unification of several groups whose ancestors moved from the territory of modern Yakutia and the Amur region. The two dominant Y-chromosome haplogroups of the Koryaks with different sublineages and haplotype clusters demonstrate their contacts with the Chukchi, Evens, Yukaghirs and Eskimos. Analysis of the composition of genetic components and IBD blocks on autosomes indicates the maximum genetic proximity of the Koryaks to the Chukchi. Among the Siberian populations, the Chukchi, Koryaks and Nivkhs form a separate cluster from the main group of Siberian populations, while the Chukchi and Koryaks are more closely related. Far Eastern populations are divided in full accordance with geographic localization into the northern group (Chukchi and Koryaks) and the southern group, including the Nivkhs and Udege. A more detailed analysis of the component composition of gene pools in some populations reveals components specific to them. The isolation of such components is associated with founder effects and a shift in allele frequencies for these populations. The Koryaks and Chukchi are one of the most striking examples of long-standing genetic kinship. In their populations, maximum values of the level of genomic inbreeding FROH 1.5 (0.0422, 0.0409) were found, which is natural due to their relative isolation.

Identifying Candida auris transmission in a hospital outbreak investigation using whole-genome sequencing and SNP phylogenetic analysis.

Candida auris poses a global public health challenge, causing multiple outbreaks within healthcare facilities. Despite advancements in strain typing for various infectious diseases, a consensus on the genetic relatedness threshold for identifying C. auris transmission in local hospital outbreaks remains elusive. We investigated genetic variations within our local isolate collection using whole-genome-based single nucleotide polymorphism (SNP) phylogenetic analysis. A total of 74 C. auris isolates were subjected to whole-genome sequencing (WGS) and SNP phylogenetic analysis via the QIAGEN CLC Genomics Workbench. Isolates included known related strains from the same patient, strains from different hospitals, strains from our hospital patients with no epidemiological link, and 19 patient isolates from a recent C. auris outbreak. All but three isolates were identified to be Clade IV. By examining the genetic diversities of C. auris within patients and between patients, we identified a SNP variation range of 0-13 for identifying related isolates. During an outbreak investigation, utilizing this range, maximum likelihood phylogenetic analysis revealed two distinct clusters that aligned with the epidemiological links. Determining a SNP variation range to delineate genetic relatedness among isolates is crucial for the application of WGS and SNP phylogenetic analysis in identifying C. auris transmission during hospital outbreak investigations. The use of WGS SNP phylogenetic analysis via the CLC Genomics Workbench has emerged as a valuable method for typing C. auris in clinical microbiology laboratories.

Molecular Typing of Pseudomonas aeruginosa Isolates Collected in Abidjan Hospitals (Côte d'Ivoire) Using the Multiple-Locus Variable Number of Tandem Repeats Method.

Background/objectives:Pseudomonas aeruginosa can cause community-acquired infections affecting various body sites. The present retrospective study investigated the genetic diversity of 173 isolates (166 clinical, 7 environmental) of P. aeruginosa collected from clinical pathology laboratories in Abidjan, Côte d'Ivoire (2001-2011). Methods: Multiple-Locus Variable Number of Tandem Repeats (VNTR) Analysis (MLVA) using 13 loci was applied to all isolates and compared to published MLVA data. The antibiotics status of the isolates was compiled when available and compared to published profiles. Results: Among 95 isolates analyzed for their antibiotics status, 14 displayed concerning resistance profiles: five multidrug-resistant (MDR) and nine extensively drug-resistant (XDR). MLVA typing revealed a high genetic diversity (>130 genotypes), with many genotypes represented by a single strain. Notably, thirteen clusters (≥4 related isolates) were observed. Some clusters displayed close genetic relatedness to isolates from France, Korea, and well-studied strains (ST560, LES and PA14). Comparative analysis suggested the presence of international high-risk MDR clones (CC233, CC111) in Côte d'Ivoire. Importantly, MLVA clustering revealed a close relationship of CC235-MDR strains with a locally identified cluster (group 9). Conclusions: These findings support MLVA as a reliable and cost-effective tool for low-resource settings, allowing the selection of relevant strains for future whole genome sequence analyses. This approach can improve outbreak investigations and public health interventions aimed at curbing MDR P. aeruginosa transmission within hospitals and at the national level.

COVID-19 and University Students' Well-Being: An Ecological and Multidimensional Perspective on Post-Pandemic Effects.

In February 2020, the Italian government started to adopt measures to contain the spread of COVID-19. This emergency had a strong impact on people's lives and daily activities, negatively affecting their well-being. One of the groups of people that suffered the most from the pandemic emergency and the related isolation was university students. Based on these considerations, this article analyzes the effects of COVID-19 on Italian students' well-being during three periods: the first lockdown (March-April 2020), one year later (March-April 2021), and two years after the lockdowns (March-April 2022). Three samples comprising a total of 765 participants (M = 21 years, SD = 2.87) completed an online self-report questionnaire, which included the I COPPE scale (its short form), a tool that measures the perception of present and future well-being, both as an overall evaluation and its six specific domains: interpersonal, community, occupational, physical, psychological, and economic. The results indicated a general trend in the well-being levels of university students from the beginning of the pandemic to 2022. Compared to 2020, in 2021, there was a sharp decline in well-being, whereas in 2022, there was an increase in well-being levels. Practical implications, limitations, and future recommendations arising from the present study are extensively discussed.

Multilayered politicized constructions of COVID-19 vaccines: A global south view of Pfizer and Sinovac

Despite their overwhelming public health benefits for pandemic response, Covid-19 vaccines have been subject to political controversy around the world. Multidisciplinary scholarship in the health and social sciences points to a wide range of factors that influence the public’s divided views of vaccines. But these factors have largely been considered in relative isolation from each other as independent influences on vaccine beliefs. In this paper, we propose a multilayered politico-psychological model of collective constructions of the Covid-19 vaccines. Borrowing from Montiel and Christie’s (2007) theoretical framework, we examine how the Covid-19 vaccines are holistically constructed in relation to interdependent social meanings across micro-level, meso-level, and macro-level socio-ecological layers. Harnessing a mixed methods research design, we apply this framework to analyze a large corpus of Filipino tweets (N = 229,236) about the Pfizer and Sinovac vaccines. Micro-level discourses feature competing individual emotions which construct Pfizer as an object of desire and Sinovac as an object of fear. Meso-level constructions invoke collective contexts of information and resource scarcity within which Pfizer is a serendipitous prize while Sinovac is a mandated responsibility. Finally, macro-level constructions embed the Covid-19 vaccines within national and international structures, framing Pfizer as a symbol of political integrity and Sinovac as an instrument of political corruption for government leaders. In conclusion, these multilayered discourses around the Covid-19 vaccines illuminate complex negotiations of agency among a Global South public during the pandemic. We discuss multilevel interventions and politico-psychological implications for public health campaigns more broadly.

The Peculiar Emergence of Mpox (Monkeypox): Directions for the Search for the Natural Reservoir and Vaccination Strategies

Background/Objectives: Mpox (monkeypox) is a zoonosis with origins in a currently unknown African reservoir. The first epidemiological accounts of mpox date back to the early 1980s, yet mpox only emerged as a pandemic threat in 2022–2023, more than 40 years later. This scenario is very different from those of other emerging diseases such as HIV and SARS, which immediately spread globally, in fully susceptible populations, starting from patients zero. Methods: We use mathematical modeling to illustrate the dynamics of mpox herd immunity in small communities in touch with the mpox natural reservoir. In particular, we employ an SEIR stochastic model. Results: The peculiar emergence of mpox can be explained by its relationship with smallpox, which was eradicated through universal mass vaccination in 1980. Mpox first emerged in small rural communities in touch with mpox’s animal reservoir and then spread globally. The relative isolation of these communities and their herd-immunity dynamics against mpox worked to delay the introduction of mpox in large urban centers. Conclusions: Mathematical modeling suggests that the search for the mpox animal reservoir would be most fruitful in communities with high mpox seroprevalence and small outbreaks. These are communities is tight contact with the mpox natural reservoir. We propose vaccinating individuals in communities in these communities to severely reduce the importation of cases elsewhere.

Potential Use of Fourier-Transform Infrared Spectroscopy as a Rapid Screening Tool for Investigating Nosocomial Outbreaks of ST-80 Vancomycin-Resistant Enterococcus faecium

Core genome single-nucleotide polymorphism phylogeny was used to characterise a nosocomial outbreak caused by ST-80 Vancomycin-resistant Enterococcus faecium (VREf). It identified 22 of 25 epidemiologically related isolates as belonging to an outbreak cluster. The use of Fourier-transform infrared (FT-IR) spectroscopy with a cluster-defining cut-off of 0.071 resulted in the correct classification of 21 of 22 phylogenetically related isolates in a single cluster. It successfully distinguished three phylogenetically unrelated isolates from the outbreak cluster, along with five ST-80 unrelated control isolates, and five isolates from a previous outbreak in May 2023, yielding only one mischaracterised environmental isolate. These findings support the potential use of FT-IR spectroscopy as a rapid screening tool to assist outbreak investigations. Notably, this study is the first to focus on the performance of FT-IR spectroscopy in the epidemiological analysis of VREf isolates with the same sequence type.

Novel oil-associated bacteria in Arctic seawater exposed to different nutrient biostimulation regimes.

The Arctic Ocean is an oligotrophic ecosystem facing escalating threats of oil spills as ship traffic increases owing to climate change-induced sea ice retreat. Biostimulation is an oil spill mitigation strategy that involves introducing bioavailable nutrients to enhance crude oil biodegradation by endemic oil-degrading microbes. For bioremediation to offer a viable response for future oil spill mitigation in extreme Arctic conditions, a better understanding of the effects of nutrient addition on Arctic marine microorganisms is needed. Controlled experiments tracking microbial populations revealed a significant decline in community diversity along with changes in microbial community composition. Notably, differential abundance analysis highlighted the significant enrichment of the unexpected genera Lacinutrix, Halarcobacter and Candidatus Pseudothioglobus. These groups are not normally associated with hydrocarbon biodegradation, despite closer inspection of genomes from closely related isolates confirming the potential for hydrocarbon metabolism. Co-occurrence analysis further revealed significant associations between these genera and well-known hydrocarbon-degrading bacteria, suggesting potential synergistic interactions during oil biodegradation. While these findings broaden our understanding of how biostimulation promotes enrichment of endemic hydrocarbon-degrading genera, further research is needed to fully assess the suitability of nutrient addition as a stand-alone oil spill mitigation strategy in this sensitive and remote polar marine ecosystem.

Evaluation of Biocides Susceptibility and Expression of Qace-Δ1 And Suge1 Genes of Pseudomonas Aeruginosa in Exposure to Biocides and Meropenem

Pseudomonas aeruginosa is a species of medical significance that plays a role in nosocomial infections, particularly those resulting from burns and wounds. Bacterial antibiotic resistance may have developed as a result of the abuse of biocides in hospitals. This study aimed to determine the minimum inhibitory concentrations (MICs) of carbapenem-resistant P. aeruginosa isolates in relation to ethylenediaminetetraacetic acid (EDTA), chlorohexidine (CHX), and resazurin dye using a microtiter plate assay. Additionally, it evaluated the expression of the efflux pump genes (qacE-Δ1 and sugE1) at sub-inhibitory concentrations of the biocides using RT-qPCR (delta delta Ct method) to improve their efflux pump system. The MICs of 24 P. aeruginosa isolates (14 isolates were resist to carbapenems and 10 isolates sensitive to carbapenems) against Chloroxylenol (Dettol) revealed that the range of MICs were (0. 125-2%). The results of MICs of Chlorohexidine (CHX) revealed that the range of inhibitory activity was (0.8-102.4 µg/ml), also the findings of MICs of EDTA demonstrated that the range of inhibitory activity was (125-2000 µg/ml). The genes involved in efflux pump (qacE -Δ1 and sugE1) were found to be significantly up-regulated in the local isolates, according to our findings.. The four isolates' sugE1 gene expression folds ranged from 1.81 to 5.39; this result was more than the fold of the control (the fold change is 1), with the meropenem isolate having the highest fold value (5.39) and sub-MIC value (8 µg/ml). Furthermore, the four isolates' qacE-Δ1 gene expression folds ranged from 2.11 to 5.01, which was higher than the fold of the control, where the highest fold value was recorded for the EDTA (5.01) with the sub-MIC value (125-500 µg/ml). Also, a high value of upregulation of the gene qacE –Δ1, during the treatment with dettol with sub-MIC (0.25-0.5%), and the fold change was 4.19. In conclusion, the present findings indicated to the significant relationship between the treatments with meropenem and the high values of gene expression of the gene sugE1 in comparison with the control and other agents, Furthermore, compared to the antibiotic and control, there was a strong correlation found between the high fold changes of the gene qacE -Δ1 with the presence of dettol and EDTA.

Whole genome sequencing and characterization of Corynebacterium isolated from the healthy and dry eye ocular surface

BackgroundThe purpose of this study was to characterize Corynebacterium isolated from the ocular surface of dry eye disease patients and healthy controls. We aimed to investigate the pathogenic potential of these isolates in relation to ocular surface health. To this end, we performed whole genome sequencing in combination with biochemical, enzymatic, and antibiotic susceptibility tests. In addition, we employed deferred growth inhibition assays to examine how Corynebacterium isolates may impact the growth of potentially competing microorganisms including the ocular pathogens Pseudomonas aeruginosa and Staphylococcus aureus, as well as other Corynebacterium present on the eye.ResultsThe 23 isolates were found to belong to 8 different species of Corynebacterium with genomes ranging from 2.12 mega base pairs in a novel Corynebacterium sp. to 2.65 mega base pairs in C. bovis. Whole genome sequencing revealed the presence of a range of antimicrobial targets present in all isolates. Pangenome analysis showed the presence of 516 core genes and that the pangenome is open. Phenotypic characterization showed variously urease, lipase, mucinase, protease and DNase activity in some isolates. Attention was particularly drawn to a potentially new or novel Corynebacterium species which had the smallest genome, and which produced a range of hydrolytic enzymes. Strikingly the isolate inhibited in vitro the growth of a range of possible pathogenic bacteria as well as other Corynebacterium isolates. The majority of Corynebacterium species included in this study did not seem to possess canonical pathogenic activity.ConclusionsThis study is the first reported genomic and biochemical characterization of ocular Corynebacterium. A number of potential virulence factors were identified which may have direct relevance for ocular health and contribute to the finding of our previous report on the ocular microbiome, where it was shown that DNA libraries were often dominated by members of this genus. Particularly interesting in this regard was the observation that some Corynebacterium, particularly new or novel Corynebacterium sp. can inhibit the growth of other ocular Corynebacterium as well as known pathogens of the eye.

Expression of Pili Genes (ebpA and ebpC) Associated with Multi Drug Resistant Enterococcus faecalis Isolates

The antibiotic susceptibility was determined for 118 clinical E. faecalis isolates , the isolates showed various levels of susceptibilities to different antibiotics and 70.5% of these isolates were multidrug resistant. The relative expression of ebpA gene in six multidrug resistance E. faecalis isolates was determined in comparison with antibiotic-sensitive isolates selected as a control sample. The isolate 21 from root canal (multi drug resistance) showed increasing in expression in comparison with antibiotic sensitive isolates of E. faecalis. The relative expression of ebpC gene in multidrug resistance E. faecalis isolates was determined in comparison with antibiotic sensitive isolates which were selected as control samples. The isolate 21 from root canal and the isolates 19 from urine (multi drug resistance) showed high expression in comparison with antibiotic sensitive isolates of E. faecalis. The expression of ebpA and ebpC genes in multi drug resistance isolates in relation to their sources showed that urine group gave a high rate of expression of ebpC followed by root canal in comparison with ebpA expression. In contrast, the vagina group gave a higher expression of ebpA than ebpC. The findings of the present investigation revealed a significant correlation (P=0.05) between the multi-drug resistance and gene expression of ebpA gene, while there is no significant (P=0.05) correlation with ebpC.

Genomics of Staphylococcus aureus and Enterococcus faecalis isolated from the ocular surface of dry eye disease sufferers

Ocular surface inflammatory disorders, such as dry eye, are becoming increasingly prevalent. Developing new treatment strategies targeting harmful bacteria could provide significant therapeutic benefits. The purpose of this study was to characterize the common ocular pathogen Staphylococcus aureus and the rarer endophthalmitis-associated species Enterococcus faecalis isolated from the ocular surface of dry eye disease patients in Norway. Together the 7 isolates (5 S. aureus and 2 E. faecalis) comprise the complete set of members of each species isolated in our previous study of the ocular microbiome of 61 dry eye sufferers. We aimed to investigate the pathogenic potential of these isolates in relation to ocular surface health. To this end, we used whole genome sequencing, multiplex PCR directed at virulence genes and antibiotic susceptibility tests encompassing clinically relevant agents. The E. faecalis isolates showed resistance to only gentamicin. S. aureus isolates displayed susceptibility to most of the tested antibiotics, except for two isolates which showed resistance to trimethoprim/sulfamethoxazole and three isolates which were resistant to ampicillin. Susceptibilities included sensitivity to several first-line antibiotics for treatment of ocular infections by these species. Thus, treatment options would be available if required. However, spontaneous resistance development to gentamicin and rifampicin occurred in some S. aureus which could be a cause for concern. Whole genome sequencing of the isolates showed genome sizes ranging from 2.74 to 2.83 Mbp for S. aureus and 2.86 Mbp for E. faecalis, which is typical for these species. Multilocus sequence typing and phylogenetic comparisons with previously published genomes, did not suggest the presence of eye-specific clusters for either species. Genomic analysis indicated a high probability of pathogenicity among all isolates included in the study. Resistome analysis revealed the presence of the beta-lactamase blaZ gene in all S. aureus isolates and the dfrG gene in two of them; while E. faecalis isolates carried the lsa(A) gene which confers intrinsic resistance to lincosamides and streptogramin A in this species. Screening for virulence factors revealed the presence of various pathogenicity associated genes in both S. aureus and E. faecalis isolates. These included genes coding for toxin production and factors associated with evading the host immune system. Some of the identified genes (tst, hylA &hylB) are suggested to be linked to the pathophysiology of dry eye disease. Lastly, the presence of specific S. aureus virulence genes was confirmed through multiplex PCR analysis.

“An Era More than Bleak”

AbstractFerenc Farkas, a remarkable twentieth-century Hungarian composer, was one of the most influential professors of composition throughout the history of the Liszt Academy of Music, Budapest. A less known chapter of his life is his involvement in the ethnicist and anti-Semitic political movements of Hungary, during the late 1930s and early 1940s. In 1940, he was among three musicians who elaborated a proposal to establish, in line with the rise of corporatism, a Hungarian Music Chamber. One of the main aims of the new organization would have been a total exclusion of Jewish musicians from all branches of Hungarian musical culture. The Chamber was never actually founded. In 1941, Farkas left Budapest for the Transylvanian city Kolozsvár (Cluj), where he was appointed professor of composition at the Conservatory. One of his first students was György Ligeti, a native Transylvanian, born into a Hungarian family suffering under the anti-Semitic legislation of the Hungarian state. As Ligeti recollected, Farkas “wanted to teach me everything he had learned from his teacher Ottorino Respighi.” Based on archival sources, this study offers new insights into the personal and professional connection between Ligeti and Farkas during the 1940s, and also follows Farkas's post-war path from relative isolation and marginalization to the elite of state socialist music culture.

Environmental bacterial load during surgical and ultrasound procedures in a Swedish small animal hospital

BackgroundEnvironmental bacteria in animal healthcare facilities may constitute a risk for healthcare-associated infections (HAI). Knowledge of the bacterial microflora composition and factors influencing the environmental bacterial load can support tailored interventions to lower the risk for HAI. The aims of this study were to: (1) quantify and identify environmental bacteria in one operating room (OR) and one ultrasound room (UR) in a small animal hospital, (2) compare the bacterial load to threshold values suggested for use in human healthcare facilities, (3) characterise the genetic relationship between selected bacterial species to assess clonal dissemination, and (4) investigate factors associated with bacterial load during surgery.Settle plates were used for passive air sampling and dip slides for surface sampling. Bacteria were identified by Matrix Assisted Laser Desorption—Time Of Flight. Antimicrobial susceptibility was determined by broth microdilution. Single nucleotide polymorphism-analysis was performed to identify genetically related isolates. Linear regression was performed to analyse associations between observed explanatory factors and bacterial load.ResultsThe bacterial load on settle plates and dip slides were low both in the OR and the UR, most of the samples were below threshold values suggested for use in human healthcare facilities. All settle plates sampled during surgery were below the threshold values suggested for use in human clean surgical procedures.Staphylococcus spp. and Micrococcus spp. were the dominating species. There was no indication of clonal relationship among the sequenced isolates. Bacteria carrying genes conveying resistance to disinfectants were revealed.Air change and compliance with hygiene routines were sufficient in the OR. No other factors possibly associated with the bacterial load were identified.ConclusionsThis study presents a generally low bacterial load in the studied OR and UR, indicating a low risk of transmission of infectious agents from the clinical environment. The results show that it is possible to achieve bacterial loads below threshold values suggested for use in human healthcare facilities in ORs in small animal hospitals and thus posing a reduced risk of HAI. Bacteria carrying genes conveying resistance to disinfectants indicates that resistant bacteria can persist in the clinical environment, with increased risk for HAI.

Between Refusal and Refuge. Queer Feminist Bookstore Savannah Bay

Feminist bookstores have played an important role in the creation of feminist spaces since the end of the 20th century. In the Netherlands, Savannah Bay is one of the last remaining in a previous network of feminist bookstores. This article explores how the bookstore manages to uphold its function as a feminist space while operating in relative isolation. The data used for this analysis consists of a series of interviews with volunteers working at Savannah Bay. This data is analysed via Bonnie Honig’s &lt;i&gt;Feminist Theory of Refusal&lt;/i&gt; (2021), which&lt;i&gt; &lt;/i&gt;connects three forms of feminist resistance within one arc of feminist refusal. Crucial to this arc is the circular movement where the women first leave the city, then organise a new way of living, and then return to the city to implement their ideas. By reading the experiences of Savannah Bay volunteers via Honig’s theory of refusal, this article analyses how the bookstore manages to uphold a feminist space while being embedded in a predominantly patriarchal public sphere. It demonstrates the complex ways in which Savannah Bay continuously negotiates its relationship to the customers and volunteers it caters for on the one hand, and a patriarchal public sphere which it seeks to reform on the other hand. Additionally, this reading extends and nuances Honig’s theoretical approach by relating it to empirical data, which raises questions about the conditions for fulfilling Honig’s feminist arc of refusal, and about the relations between the various moments on the arc.