Isolates Of Colletotrichum Graminicola Research Articles

A Method for Genetic Analysis ofGlomerella graminicola(Colletotrichum graminicola) from Maize

Strains derived from nine different isolates of Colletotrichum graminicola from maize participated in the production of perithecia when incubated on pieces of autoclaved corn leaves in a humidity chamber. Matings occurred between self-fertile and self-sterile strains, and also between certain self-sterile strains. As many as 200 ascospore progeny were recovered easily from individual perithecia. Characterization of progeny showed that sexual recombination and Mendelian segregation of distinct traits could be detected. Segregation of markers for chlorate resistance (Chl R ), benomyl resistance (Bml R ), and melanin deficiency (Mel − ) approximated a 1:1 ratio and defined three separate linkage groups (...)

Effect of Illuminance on the Resistance of Inbred Lines of Corn to Isolates ofColletotrichum graminicola

Effect of Illuminance on the Resistance of Inbred Lines of Corn to Isolates of<i>Colletotrichum graminicola</i>

FACTORS INFLUENCING PATHOGENICITY OF THREE ISOLATES OF COLLETOTRICHUM GRAMINICOLA ON WHEAT.

Factors such as varieties of wheat, hydrogen ion and various mineral salts concentrations of soil having possible influence on the pathogenicity of certain isolates ofColletotrichum graminicola (Ces.)Wils. on wheat were studied. Of the six varieties of wheat studied, the variety Dual was highly susceptible, and the variety Knox highly resistant. The percentage seedling blight was greatest in soil at the highest pH values, 7.0–9.0. Concentrations of potassium nitrate, ammonium nitrate and magnesium sulfate in soil greatly influenced disease development.

FACTORS INFLUENCING FORMATION OF ACERVULI AND CONIDIA BY COLLETOTRICHUM GRAMINICOLA.

Abundant sporulation of certain isolates ofColletotrichum graminicola (Ces.)Wils., occurred when sufficient aeration was provided to their liquid culture either by reducing the quantity of the media or by forcing air into them. Certain carbon and nitrogen compounds caused more sporulation of only orchard grass isolate of the fungus. When sufficient aeration was provided to the culture media more sporulation also occurred at a temperature range of 20–30°C. Glucose content of the media did not have any influence on sporulation.

Comparison of the physiology of three isolates of Colletotrichum graminicola.

Three isolates ofColletotrichum graminicola (Ces.)Wils. (from wheat, orchard grass and alfalfa) were used in an investigation of their comparative physiology. The carbon compounds, lactose, sucrose, d-mannitol, d(+) mannose, d(+) raffinose, dextrose and d(-) levulose and the nitrogen compounds, DL-histidine, L(+) asparagine, L-arginine, DL-tryptophane, ammonium nitrate and Difco Yeast extract were utilized by all three isolates. Differences were noticed only in the amounts utilized. The utilization of certain carbon and nitrogen compounds by the isolates depended on their precultural substrates light, and concentration of glucose in the medium.

Multiple temperature optima in collectotrichum graminicola

The dry weights of three isolates ofColletotrichum graminicola (Ces.)Wills., growing at 10°, 15°, 20°, 30°, and 35° C in yeast extract liquid medium were recorded. Two temperature growth optima and minima occurred at 20°C, 30°C and 10°C, 25°C respectively.