Smart control techniques have been implemented to address fluctuating power levels within isolated microgrids, mitigating the risk of unstable frequencies and the potential degradation of power supply quality. However, a challenge lies in the fact that employing these computationally complex methods without stability preservation might not suffice to handle the rapid changes of this highly dynamic environment in real-world scenarios over communication delays. This study introduces a flexible real-time approach for the frequency control problem using an artificial neural network (ANN) constrained to stabilized regions. Our solution integrates stabilizing PID controllers, computed through small-signal analysis and tuned via an automated search for optimal ANN weights and reinforcement learning (RL)-based selected constraints. First, we design stabilizing PID controllers by applying the stability boundary locus method and the Mikhailov criterion, specifically addressing communication delays. Next, we refine the controller parameters online through an automated process that identifies optimal coefficient combinations, leveraging a constrained ANN to manage frequency deviations within a restricted parameter range. Our approach is further enhanced by employing the RL technique, which trains the tuning system using an interpolated stability boundary curve to ensure both stability and performance. This one-of-a-kind combination of ANN, RL, and advanced PID tuning methods is a big step forward in how we handle frequency control problems in isolated AC microgrids. The experiments show that our solution outperforms traditional methods due to its reduced parameter search space. In particular, the proposed method reduces transient and steady-state frequency deviations more than semi- and unconstrained methods. The improved metrics and stability analysis show that the method improves system performance and stability under changing conditions.