Iron Carrier Research Articles

Antagonism of rhizosphere Trichoderma brevicompactum DTN19 against the pathogenic fungi causing corm rot in saffron (Crocus sativus L.) in vitro.

Corm rot in saffron (Crocus sativus L.) significantly impacts yield and quality. Non-toxic fungi, particularly Trichoderma species, are valuable for biological control due to their production of diverse and biologically active secondary metabolites. This study aimed to isolate an effective antagonistic fungus against the pathogenic fungi causing corm rot in saffron. Four pathogenic fungi (Fusarium oxysporum, Fusarium solani, Penicillium citreosulfuratum, and Penicillium citrinum) were isolated from diseased saffron bulbs in Chongming. Initial screening through dual culture with these pathogens re-screening from rhizosphere soil samples of C. sativus based on its inhibitory effects through volatile, nonvolatile, and fermentation broth metabolites. The inhibitory effect of biocontrol fungi on pathogenic fungi in vitro was evaluated by morphological observation and molecular biology methods. Antagonistic fungi were identified as Trichoderma brevicompactum DTN19. F. oxysporum was identified as the most severe pathogen. SEM (scanning electron microscope) and TEM (transmission electron microscope) observations revealed that T. brevicompactum DTN19 significantly inhibited the growth and development of F. oxysporum mycelium, disrupting its physiological structure and spore formation. Additionally, T. brevicompactum DTN19 demonstrated nitrogen fixation and production of cellulase, IAA (Indole acetic acid), and siderophores. Whole-genome sequencing of strain DTN19 revealed genes encoding protease, cellulase, chitinase, β-glucosidase, siderophore, nitrogen cycle, and sulfate transporter-related proteins. T. brevicompactum DTN19 may inhibit the propagation of pathogenic fungi by destroying their cell walls or producing antibiotics. It can also produce IAA and iron carriers, which have the potential to promote plant growth. Overall, T. brevicompactum DTN19 showed the development prospect of biological agents.

Magnetic-supported catalyst derived from red mud-based Fe-Co PBA for efficient degradation of organic pollutants by activating peroxymonosulfate under visible light

To alleviate the environmental pressure caused by the continuous accumulation of solid waste red mud (RM) and organic pollutants, we developed a magnetic-supported catalyst derived from RM-based Fe-Co Prussian blue analogues (RM-Co PBA-D) using RM as the iron source and carrier. Then, the catalyst was used to activate peroxymonosulfate (PMS) under visible light for the degradation of tetracycline (TC) and rhodamine B (RhB). Due to the superior dispersion, strong visible light response, and high photo-generated carrier separation efficiency of the RM-Co PBA-D, the RM-Co PBA-D/PMS/Vis system could completely degrade both TC (10 mg/L) and RhB (20 mg/L) in 30 min, with mineralization efficiencies as high as 70.12 % and 60.59 %, respectively. Several reactive oxygen species (ROS) such as SO4⋅−, ⋅OH−, ⋅O2−, 1O2, and h+ were involved in the degradation process, with SO4⋅− and 1O2 playing a dominant role. More importantly, except for the production of ROS, the regeneration of active sites (Co2+/Fe2+) mediated by photo-generated carriers was also a critical factor in enhancing the removal of organic pollutants. In addition, the constructed system demonstrated significant potential for practical applications, which was attributed to the stable structure and catalytic performance of the catalyst as well as the strong ability of the system to resist interference by impurity anions and detoxify organic pollutants.

Structure of Aquifex aeolicus lumazine synthase by cryo-electron microscopy to 1.42 Å resolution.

Single-particle cryo-electron microscopy (cryo-EM) has become an essential structural determination technique with recent hardware developments making it possible to reach atomic resolution, at which individual atoms, including hydrogen atoms, can be resolved. In this study, we used the enzyme involved in the penultimate step of riboflavin biosynthesis as a test specimen to benchmark a recently installed microscope and determine if other protein complexes could reach a resolution of 1.5 Å or better, which so far has only been achieved for the iron carrier ferritin. Using state-of-the-art microscope and detector hardware as well as the latest software techniques to overcome microscope and sample limitations, a 1.42 Å map of Aquifex aeolicus lumazine synthase (AaLS) was obtained from a 48 h microscope session. In addition to water molecules and ligands involved in the function of AaLS, we can observe positive density for ∼50% of the hydrogen atoms. A small improvement in the resolution was achieved by Ewald sphere correction which was expected to limit the resolution to ∼1.5 Å for a molecule of this diameter. Our study confirms that other protein complexes can be solved to near-atomic resolution. Future improvements in specimen preparation and protein complex stabilization may allow more flexible macromolecules to reach this level of resolution and should become a priority of study in the field.

A Bacillus velezensis strain isolated from oats with disease-preventing and growth-promoting properties

Endophytes have been shown to promote plant growth and health. In the present study, a Bacillus velezensis CH1 (CH1) strain was isolated and identified from high-quality oats, which was capable of producing indole-3-acetic acid (IAA) and strong biofilms, and capabilities in the nitrogen-fixing and iron carriers. CH1 has a 3920 kb chromosome with 47.3% GC content and 3776 code genes. Compared genome analysis showed that the largest proportion of the COG database was metabolism-related (44.79%), and 1135 out of 1508 genes were associated with the function “biosynthesis, transport, and catabolism of secondary metabolites.” Furthermore, thirteen gene clusters had been identified in CH1, which were responsible for the synthesis of fifteen secondary metabolites that exhibit antifungal and antibacterial properties. Additionally, the strain harbors genes involved in plant growth promotion, such as seven putative genes for IAA production, spermidine and polyamine synthase genes, along with multiple membrane-associated genes. The enrichment of these functions was strong evidence of the antimicrobial properties of strain CH1, which has the potential to be a biofertilizer for promoting oat growth and disease resistance.

Construction of ATMT system of Fusarium oxysporum and evaluation of its promoting effect on Glycyrrhiza uralensis

This study aims to evaluate the in vivo function of Fusarium oxysporum in Glycyrrhiza uralensis by salt tolerance,indoleacetic acid(IAA) production capacity, phosphate-dissolving capacity, and iron carrier production capacity. The stable genetic transformation system of the F. oxysporum was established by Agrobacterium tumefaciens-mediated genetic transformation( ATMT)technology, and the stability and staining efficiency of transformants were detected by the cloning of the marker gene green fluorescent protein(GFP) and the efficiency of β-glucuronidase staining(GUS). Efficient and stable transformants were selected for restaining G. uralensis and evaluating its influence on the growth of the G. uralensis seedlings. The results show that F. oxysporum has good salt tolerance and could still grow on potato glucose agar(PDA) medium containing 7% sodium chloride, but the growth rate slows down with the increase in sodium chloride content in PDA medium. F. oxysporum has the function of producing indoleacetic acid, and the concentration of IAA in its fermentation broth is about 3. 32 mg · m L~(-1). In this study, the genetic transformation system of F. oxysporum is successfully constructed, and the ATMT system is efficient and stable. One transformant with both high staining efficiency and genetic stability is selected, and the restaining rate of the transformant in G. uralensis is 76. 92%, which could significantly improve the main root length of one-month-old G. uralensis seedlings and promote the growth and development of G. uralensis seedlings. The results of this study can lay the foundation for the development of biological bacterial fertilizer and the growth regulation of high-quality G. uralensis.

The Effect of Two Siderophore-Producing Bacillus Strains on the Growth Promotion of Perennial Ryegrass under Cadmium Stress.

Cadmium (Cd) is a highly toxic and cumulative environmental pollutant. Siderophores are heavy metal chelators with high affinity to heavy metals, such as Cd. Ryegrass (Lolium perenne L.) has a potential remediation capacity for soils contaminated by heavy metals. Consequently, using ryegrass alongside beneficial soil microorganisms that produce siderophores may be an effective means to remediate soils contaminated with Cd. In this study, the Bacillus strains WL1210 and CD303, which were previously isolated from the rhizospheres of Nitraria tangutorum in Wulan and Peganum harmala L. in Dachaidan, Qinghai, China, respectively, both arid and sandy environments, were evaluated for heavy metal pollution mitigation. Our quantitative analyses have discerned that the two bacterial strains possess commendable attributes of phosphorus (P) solubilization and potassium (K) dissolution, coupled with the capacity to produce phytohormones. To assess the heavy metal stress resilience of these strains, they were subjected to a cadmium concentration gradient, revealing their incremental growth despite cadmium presence, indicative of a pronounced tolerance threshold. The subsequent phylogenetic analysis, bolstered by robust genomic data from conserved housekeeping genes, including 16S rDNA, gyr B gene sequencing, as well as dnaK and recA, delineated a species-level phylogenetic tree, thereby confirming the strains as Bacillus atrophaeus. Additionally, we identified the types of iron-carrier-producing strains as catechol (WL1210) and carboxylic acid ferrophilin (CD303). A genomic analysis uncovered functional genes in strain CD303 associated with plant growth and iron carrier biosynthesis, such as fnr and iscA. Ryegrass seed germination assays, alongside morphological and physiological evaluations under diverse heavy metal stress, underscored the strains' potential to enhance ryegrass growth under high cadmium stress when treated with bacterial suspensions. This insight probes the strains' utility in leveraging alpine microbial resources and promoting ryegrass proliferation.

Transcriptional regulation of the yersiniabactin receptor fyuA gene by the ferric uptake regulator in Klebsiella pneumoniae NTUH-K2044.

The ferric uptake regulator (Fur) is a global regulator that influences the expression of virulence genes in Klebsiella pneumoniae. Bioinformatics analysis suggests Fur may involve in iron acquisition via the identified regulatory box upstream of the yersiniabactin receptor gene fyuA. To observe the impact of the gene fyuA on the virulence of K. pneumoniae, the gene fyuA knockout strain and complementation strain were constructed and then conducted a series of phenotypic experiments including chrome azurol S(CAS) detection, crystal violet staining, and wax moth virulence experiment. To examine the regulatory relationship between Fur and the gene fyuA, green fluorescent protein (GFP) reporter gene fusion assay, real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), gel migration assay (EMSA), and DNase I footprinting assay were used to clarify the regulatory mechanism of Fur on fyuA. CAS detection revealed that the gene fyuA could affect the generation of iron carriers in K. pneumoniae. Crystal violet staining experiment showed that fyuA could positively influence biofilm formation. Wax moth virulence experiment indicated that the deletion of the fyuA could weaken bacterial virulence. GFP reporter gene fusion experiment and RT-qPCRanalysis revealed that Fur negatively regulated the expression of fyuA in iron-sufficient environment. EMSA experiment demonstrated that Fur could directly bind to the promoter region of fyuA, and DNase I footprinting assay further identified the specific binding site sequences. The study showed that Fur negatively regulated the transcriptional expression of fyuA by binding to upstream of the gene promoter region, and then affected the virulence of K. pneumoniae.

Analysis of clinical characteristics of infections caused by Shewanella species

PurposeThe Shewanella genus is a rare pathogen of marine origin. In recent years, there has been a continuous increase in infection cases caused by this bacterium, and we have observed the uniqueness of infections caused by this microorganism. Materials and methodsThis study conducted a retrospective analysis of the medical history and laboratory examination data of patients infected with the Shewanella genus over the past decade. Additionally, it employed bioinformatics methods to analyze the relevant virulence factors and antibiotic resistance genes associated with the Shewanella genus. ResultsOver the past 10 years, we have isolated 51 cases of Shewanella, with 68.82% being Shewanella putrefaciens (35/51 cases) and 31.37% being Shewanella algae (16/51 cases). Infected individuals often had underlying diseases, with 39.22% (20/51) having malignant tumors and 25.49% (13/51) having liver and biliary system diseases primarily characterized by stones. The majority of patients, 62.74% (32/51), exhibited mixed infections, including one case with a combination of infections from three other types of bacteria and five cases with a combination of infections from two other types of bacteria. The identified microorganisms were commonly resistant to ticarcillin-clavulanic acid (23.5%), followed by cefoperazone-sulbactam (19.6%), ciprofloxacin (17.6%), and cefotaxime (17.6%). Bioinformatics analysis indicates that Shewanella can express bile hydrolysis regulators and fatty acid metabolism regulators that aid in adapting to the unique environment of the biliary tract. Additionally, it expresses abundant catalase, superoxide dismutase, and two-component signal transduction system proteins, which may be related to environmental adaptation. Shewanella also expresses various antibiotic resistance genes, including beta-lactamases and aminoglycoside modification enzymes. Iron carriers may be one of its important virulence factors. ConclusionsWe speculate that the Shewanella genus may exist as a specific colonizer in the human body, and under certain conditions, it may act as a pathogen, leading to biliary infections in the host.

Deciphering Iberian Variscan Orogen Magmatism Using the Anisotropy of Magnetic Susceptibility from Granites

In this paper, we have synthesized the information derived from more than 20 papers and PhD theses on the anisotropy of the magnetic susceptibility (AMS) of 19 Variscan granite plutons, spanning the period between 320 Ma and 296 Ma. The AMS data are obtained from 876 sampling sites with more than 7080 AMS measurements and a re-interpretation is proposed. The studied granites exhibit a magnetic susceptibility (Km) ranging from 30 to 10,436 × 10−6 SI units. Most granites typically exhibit Km values below 1000 × 10−6 SI, indicative of paramagnetic behavior. Biotite serves as the main carrier of iron (Fe), emphasizing the reduced conditions prevalent during the formation of granite melts in the Variscan orogeny. The AMS fabrics of the studied granite plutons record the magma strain, expressing the chronologic evolution of the stress field during the orogeny. This chronologic approach highlights the magmatic events between around 330 and 315 Ma, occurring in an extensional regime, in which the Borralha pluton is an example of a suite that recorded this extensional AMS fabric. Plutons with ages between 315 and 305 Ma show AMS fabrics, pointing out their emplacement in a compressional tectonic regime related to the Variscan collision. The plutons, younger than 305 Ma, record AMS fabrics indicating that the tectonic setting for emplacement changes from a wrench regime to an extensional one at the end of the collision stage. This is evident as there is a chronological overlap between the granites that exhibit AMS fabrics indicating extension and the ones that have AMS fabrics indicating a wrench regime.

The Influence of Current Magnitudes and Profiles on the Sedimentation of Magnetorheological Fluids: An Experimental Work

Magnetorheological fluids (MRFs) are widely used for various kinds of controllable devices since their properties can be controlled by an external magnetic field. Despite many benefits of MRFs, such as fast response time, the sedimentation arisen due to the density mismatch of the compositions between iron particles and carrier oil is still one of bottlenecks to be resolved. Many studies on the sedimentation problem of MR fluids have been carried out considering appropriate additives, nanoparticles, and several carrier oils with different densities. However, a study on the effect of current magnitudes and profiles on the sedimentation is considerably rare. Therefore, this study experimentally investigates sedimentation behaviors due to different current magnitudes and different magnitude profiles such as square and sine waves in different diameters. The evaluation was performed by visual observation to obtain the sedimentation rate. It was found that the average sedimentation rate of the square type of current is slower compared to the sinusoidal type. It has also been identified that the higher intensity of the applied current results in a stronger electromagnetic field, which could slow down the sedimentation. The results achieved in this work can be effectively used to reduce particle sedimentation in the controller design of various application systems utilizing MRFs in which the controller generates a different magnitude and different profile of the external magnetic field.

Characteristics of bacterial communities in rhizosphere and bulk soil in Fe-deficient citrus growing in coastal saline-alkali land.

Citruses often occur with imbalance in iron nutrition in coastal saline-alkali lands, which severely limits the yield and quality of the fruit. In the rhizosphere, the salt content plays a crucial role in reducing uptake of iron, as well as the activity and abundance of bacteria. However, few studies have explored how salt content affects the effectiveness of iron and the community structure of bacteria across different vertical spatial scales. We investigated the citrus rhizosphere (0-30 cm) and bulk (0-60 cm) soil microenvironments of the coastal saline soil were analyzed using the 16S rRNA amplicon and inductively coupled plasma-optical emission spectroscopy. We found that the nutrient-related elements in the rhizosphere and bulk soil decreased with increasing soil depth, while the salinity-related elements showed the opposite trend. The nutrient-related element content in the rhizosphere was higher than that in the bulk, whereas the salinity-alkaline-related element content was lower than that in the bulk. The structure and diversity of bacterial communities are affected by the rhizosphere and soil depth. In the bulk, there are enriched bacteria such as WB1-A12, Nitrospiraceae and Anaerolineae that are tolerant to salt-alkali stress. In the rhizosphere, bacteria that promote plant nutrient absorption and secretion of iron carriers, such as Pseudomonas, Streptomyces, and Duganella, are prominent. The soil depth and rhizosphere affect soil nutrients and saline alkali-related factors. Changes in soil depth and rhizosphere determine the structure and diversity of bacterial communities. Rhizosphere enhances iron absorption promoting bacteria to alleviate iron deficiency stress in saline-alkali soils. Our results indicate that citrus roots maybe can resist the stress of iron deficiency in saline-alkali soils by enhancing iron absorption promoting bacteria.

METTL3 regulates TFRC ubiquitination and ferroptosis through stabilizing NEDD4L mRNA to impact stroke

BackgroundStroke is a major medical problem, and novel therapeutic targets are urgently needed. This study investigates the protective role and potential mechanisms of the N6-methyladenosine (m6A) RNA methyltransferase METTL3 against cerebral injury resulting from insufficient cerebral blood flow.MethodsIn this study, we constructed mouse MCAO models and HT-22 cell OGD/R models to mimic ischemic stroke-induced brain injury and neuronal damage. We generated NEDD4L knockout and METTL3 overexpression models and validated therapeutic effects using infarct volume, brain edema, and neurologic scoring. We performed qRT-PCR, western blotting, and co-immunoprecipitation to assess the influence of NEDD4L on ferroptosis markers and TFRC expression. We verified the effect of NEDD4L on TFRC ubiquitination by detecting half-life and ubiquitination. Finally, we validated the impact of METTL3 on NEDD4L mRNA stability and MCAO outcomes in both in vitro and in vivo experimental models.ResultWe find NEDD4L expression is downregulated in MCAO models. Overexpressing METTL3 inhibits the iron carrier protein TFRC by upregulating the E3 ubiquitin ligase NEDD4L, thereby alleviating oxidative damage and ferroptosis to protect the brain from ischemic injury. Mechanistic studies show METTL3 can methylate and stabilize NEDD4L mRNA, enhancing NEDD4L expression. As a downstream effector, NEDD4L ubiquitinates and degrades TFRC, reducing iron accumulation and neuronal ferroptosis.ConclusionIn summary, we uncover the METTL3-NEDD4L-TFRC axis is critical for inhibiting post-ischemic brain injury. Enhancing this pathway may serve as an effective strategy for stroke therapy. This study lays the theoretical foundation for developing m6A-related therapies against ischemic brain damage.

Pseudomonas weihenstephanensis through the iron metabolism pathway promotes in situ spoilage capacity of prepared beef steaks during cold storage

In this study, we evaluated the histomorphology, reactive oxygen species (ROS), protein degradation, and iron metabolism characteristics and differential expression analysis of genes for siderophores synthesis and protease secretion in prepared beef steaks inoculated alone or co-inoculated with P. weihenstephanensis, B. thermotrichothrix and M. caseolyticus at 4 °C for 12 days. The results showed that the P. weihenstephanensis was the key bacteria that degraded protein in the process of prepared beef steaks spoilage, which led to protein oxidation by promoting ferritin degradation to release free iron and inducing ROS accumulation. The highest expression of FpvA and AprE was detected in the P. weihenstephanensis group by comparing qRT-PCR of the different inoculation groups. Both qRT-PCR and Western blot revealed that ferritin heavy polypeptide and ferritin light chain polypeptide gene and protein expressions were significantly higher in the P. weihenstephanensis inoculation group compared to the other inoculation groups. Results suggested that FpvA and AprE might play roles in meat spoilage and were potential positional, physiological and functional candidate genes for improving the quality traits of prepared beef steaks. This work may provide insights on controlling food quality and safety by intervening in spoilage pathways targeting iron carrier biosynthesis or protease secretion genes.

Anti-sedimentation mechanism of rotary magnetorheological brake integrating multi-helix microstructure

Solving the sedimentation problem of magnetorheological (MR) fluids is the key to promoting the application of MR actuators for long-term storage. Current strategies for mitigating MR fluid sedimentation focus on improving the material constitution of MR fluids. However, the sedimentation is bound to emerge over time as a result of the density mismatch between carbonyl iron particles and carrier liquids. Therefore, more robust approach to solve the particle sedimentation should be considered, such as improving the robustness of the MR actuator. This paper proposes a novel anti-sedimentation MR brake (AS-MRB) that integrates a multi-helix microstructure on the shaft surface. The protrusion-shaped multi-helix microstructure applied a combination of shear stress, pressure, friction, and centrifugal force to MR fluids. Therefore, the AS-MRB can autonomously alter the internal flow state, thus performing the self-homogenization of sedimented particles. Remarkably, vortexes with axis displacement were generated in the MR working gap during the self-homogenization process. This phenomenon facilitated the mass transfer between different fluid layers, as confirmed by the speed reduction and torque test. To accurately describe the real-time rotational speed of the AS-MRB, an impact-introduced dynamic model of the shaft was also proposed. An inductance sensor-based test system was developed to characterize the volume fraction of the particle instantaneously, which is directly pertinent to the evaluation of the anti-sedimentation performance. Severely sedimented MR fluids were introduced into the AS-MRB for the performance evaluation in harsh conditions. The results show that the maximum volume fraction difference Δφmax of the sedimented MR fluid was decreased from 54.02 % to 2.46 % after the homogenization. The maximum shear stress τmax of the sedimented MR fluid was increased from 11.78 % to 80.7 % of that of the initial MR fluid. These demonstrate the strong anti-sedimentation performance of the AS-MRB. Consequently, this study has developed a unique method for solving the sedimentation problem of MR fluids and creates avenues for promoting the application of MR actuators in long-term storage.

NGAL Mediates Anaplastic Thyroid Carcinoma Cells Survival Through FAS/CD95 Inhibition.

Neutrophil gelatinase-associated lipocalin (NGAL), a siderophore-mediated iron binding protein, is highly expressed in human anaplastic thyroid carcinomas (ATCs) where it plays pleiotropic protumorigenic roles including that of a prosurvival protein. Here we show that NGAL inhibits FAS/CD95 death receptor to control ATC cell survival. FAS/CD95 expression in human specimens from patients with ATC and in ATC-derived cell lines negatively correlate with NGAL expression. Silencing of NGAL in ATC cells leads to FAS/CD95 upregulation, whereas NGAL overexpression determines the opposite effect. As a result, an agonist anti-FAS/CD95 antibody induces cell death in NGAL-silenced cells while it is ineffective on NGAL-overexpressing cells. Interestingly, the inhibitory activity of NGAL on FAS/CD95 is due to its iron carrier property given that perturbing iron homeostasis of NGAL-proficient and -deficient ATC cells directly influences FAS/CD95 expression. Accordingly, conditioned media containing a mutant form of NGAL unable to bind siderophores cannot rescue cells from FAS/CD95-dependent death, whereas NGAL wild type-containing conditioned media abolish the effects of the agonist antibody. We also find that downregulation of FAS/CD95 expression is mediated by iron-dependent NGAL suppression of p53 transcriptional activity. Our results indicate that NGAL contributes to ATC cell survival by iron-mediated inhibition of p53-dependent FAS/CD95 expression and suggest that restoring FAS/CD95 by NGAL suppression could be a helpful strategy to kill ATC cells.

Physiological Characteristics and Nitrogen and Iron Utilization of Marine Polycultures and Bundles of Hair Algae

In response to the problem of iron limitation in nitrogen-fixing cyanobacteria, this study explores the potential for symbiotic and mutually beneficial community survival of Polychaeta and Trichoderma. The presence of the two elements and their effects on the survival of the cyanobacteria were first analysed in the context of nitrogen and iron use and cycling in the ocean. The physiological characteristics of the two algae were also analysed in relation to environmental factors such as light, ph and temperature in the marine water column. 730The results of the incubation experiments showed that the filtrate of Trichoderma reesei had a significant effect on the growth of Polycoccus, with the OD730 rising to 0.0372 within 72 hours of the addition of Trichoderma reesei. The addition of the wild-type iron carrier secretion of G. globulus also promoted the growth rate and nitrogen fixation efficiency of the algae. The symbiotic relationship between the nitrogen and iron utilisation of the algae was shown to contribute to the growth and nitrogen fixation activities of both species.

Assembly of Tomato Rhizobacteria from Different Functional Groups Improves Seedling Photosynthesis and Growth

The rhizosphere harbors abundant plant growth-promoting rhizobacteria (PGPR) that are vital for plant health. In this study, we screened growth-promoting bacteria from tomato rhizosphere soil, verified their functions, and constructed the optimal combination of growth-promoting bacteria for promoting tomato growth. Furthermore, the effects of these bacteria on various physiological and biochemical parameters of tomato plants were evaluated. A total of 36 strains of rhizobacteria were isolated from tomato rhizosphere soil and their abilities to produce indole-3-acetic acid (IAA), solubilize phosphate and iron carriers were assessed. The bacterial strains with the highest capacities for IAA production (R62, R317), phosphate solubilization (R41, R219), and siderophore production (R25, R325) were selected to form three bacterial combinations: R62 + R219 + R317 + R325 (T1), R62 + R325 (T5), and R317 + R325 (T8). Fifteen days after inoculation, all three combinations showed a stimulatory effect on seedling growth compared to the un-inoculated control. Inoculation with T1, T5 and T8 increased the seedling vigor index by 173.7%, 204.1%, and 168.7%, respectively. Compared to the un-inoculated control, the T1 combination increased the activities of polyphenol oxidase, peroxidase, and the net photosynthetic rate by 132.7%, 18.7%, 58.5%, and upregulated the relative expression levels of the photosynthetic assimilation-related genes RbcL, RbcS, FBPase and FDA by 22.2-, 6.6-, 1.95-, and 2.0-fold, respectively. Our findings provide a potential for constructing rhizobacterial combinations of different functional groups for improving crop growth.

Potential of a novel endophytic diazotrophic Serratia sp. Wed4 for pyrene biodegradation

Endophytic diazotrophic Serratia sp. Wed4 with high nitrogenase activity (1260.9 ± 8.3 nmol C2H4 mL−1h−1) was isolated from Glycine max Linn growing in PAH contaminated soil for improving PAH removal and crop growth. Strain Wed4 biodegraded 37.0% of pyrene within 14 d via phthalate pathway in vitro and could biodegraded other PAHs with 2–5 rings effectively. Its capabilities on promoting crop growth included indole acetic acid (IAA) production (30.8 ± 3.3 μg mL−1), iron carrier production (A/Ar = 0.841 ± 0.002), inorganic phosphate dissolution (D/d = 1.184 ± 0.032) and organic phosphate dissolution (D/d = 1.154 ± 0.025). Strain Wed4 successfully colonized in barley tissues (>104 CFU g−1 FW), significantly promoted barley growth and nitrogen nutrition, reduced pyrene residues especially in shoots. Under sufficient nitrogen condition, strain Wed4 significantly promoted the dry weight of barley by 18.9%–33.9%, and decreased the pyrene residues by 46.4% in shoots and by 32.1% in roots. Meanwhile, under the deficient nitrogen condition, strain Wed4 significantly promoted the dry weight of barley by 40.1%–63.3% and decreased the pyrene residues by 39.0% in shoots and by 28.0% in roots. The pyrene contents were significantly negatively correlated with the total nitrogen contents in barley tissues, suggesting that nitrogen fixation of strain Wed4 also stimulated the pyrene removal. Based on its performance, Serratia sp. Wed4 provided a great potential to increasing crop yield and reducing PAH pollution in nitrogen deficient soils.

Promotional Properties of ACC Deaminase-Producing Bacterial Strain DY1-3 and Its Enhancement of Maize Resistance to Salt and Drought Stresses.

Salt stress and drought stress can decrease the growth and productivity of agricultural crops. Plant growth-promoting bacteria (PGPB) may protect and promote plant growth at abiotic stress. The aim of this study was to search for bacterial strains that can help crops resist rises in drought and salt stresses, to improve crop seed resistance under drought and salt stresses, and to investigate the effect of bacterial strains that can help crop resist external stresses under different stress conditions. Pseudomonas DY1-3, a strain from the soil under the glacier moss community of Tien Shan No. 1, was selected to investigate its growth-promoting effects. Previous studies have shown that this strain is capable of producing ACC (1-aminocyclopropane-1-carboxylic acid) deaminase. In this experiment, multifunctional biochemical test assays were evaluated to determine their potential as PGPB and their bacterial growth-promoting properties and stress-resistant effects on maize plants were verified through seed germination experiments and pot experiments. The results showed that strain DY1-3 has good salt and drought tolerance, as well as the ability to melt phosphorus, fix nitrogen, and produce iron carriers, IAA, EPS, and other pro-biomasses. This study on the growth-promoting effects of the DY1-3 bacterial strain on maize seeds revealed that the germination rate, primary root length, germ length, number of root meristems, and vigor index of the maize seeds were increased after soaking them in bacterial solution under no-stress, drought-stress, and salt-stress environments. In the potting experiments, seedlings in the experimental group inoculated with DY1-3 showed increased stem thicknesses, primary root length, numbers of root meristems, and plant height compared to control seedlings using sterile water. In the study on the physiological properties of the plants related to resistance to stress, the SOD, POD, CAT, and chlorophyll contents of the seedlings in the experimental group, to which the DY1-3 strain was applied, were higher than those of the control group of seedlings to which the bacterial solution was not applied. The addition of the bacterial solution reduced the content of MDA in the experimental group seedlings, which indicated that DY1-3 could positively affect the promotion of maize seedlings and seeds against abiotic stress. In this study, it was concluded that strain DY1-3 is a valuable strain for application, which can produce a variety of pro-biotic substances to promote plant growth in stress-free environments or to help plants resist abiotic stresses. In addition to this, the strain itself has good salt and drought tolerance, making it an option to help crops grown in saline soils to withstand abiotic stresses, and a promising candidate for future application in agricultural biofertilizers.

Developing garlic polysaccharide-Fe (III) complexes using garlic pomace to provide enhanced iron-supplementing activity in vivo

This study investigated the potential of garlic polysaccharides (GPs) from garlic pomace as iron carriers. The obtained GP-Fe (III) complexes had a higher molecular weight (5646 Da) and more fructose (90.46 %) than the GPs did and contained 9.7 % Fe (III). GPs were mainly composed of → 2)-β-d-Fruf (1 → and → 2)-β-d-Fruf (6 → residues, and their interactions with Fe (III) reduced the crystallinity, increased the thermal stability, and altered the morphological features through targeting the OH stretching vibrations of the hydroxyl groups and affecting the COC and OCO structures. The GP-Fe (III) complexes had high stability under simulated gastrointestinal digestion system and showed better therapeutic effects on iron deficiency anemia in mice than FeSO4 did, evidenced by improved hematological parameters, restored iron levels, and attenuated oxidative damage. Thus, GP-Fe (III) complexes are promising as novel Fe (III) supplements for Fe-deficient individuals, and promote the high-value utilization of garlic pomace.