Iranian Medicinal Plants Research Articles

Unveiling the Larvicidal Potential of Golpar (Heracleum persicum Desf. ex Fisch.) Essential Oil and Its Main Constituents on Aedes and Anopheles Mosquito Vectors

Natural products are thoroughly studied as valuable alternatives to synthetic insecticides. Heracleum persicum Desf. ex Fisch. (Apiaceae), commonly known as Golpar, is an Iranian medicinal plant largely employed as a spice, which has previously revealed insecticidal potential. The chemical composition of H. persicum essential oil (EO) was investigated by GC-MS and was mainly dominated by hexyl butyrate (36.1%) and octyl acetate (23.7%). The EO and its main esters were tested on three mosquito species. Aedes aegypti (L.) larvae were the most sensitive to all tested products. Lethal concentrations (LC50) of 59.09, 53.59, and 47.05 ppm were recorded for the EO, hexyl butyrate, and octyl acetate, respectively. Aedes albopictus (Skuse) and Anopheles gambiae Giles demonstrated comparable sensitivity to the EO, with LC50 values of 102.97 and 97.91 ppm, respectively, whereas the isolated constituents appeared more active on An. gambiae (LC50 of hexyl butyrate and octyl acetate of 70.97 and 60.71 ppm, respectively) with respect to Ae. albopictus (LC50 of hexyl butyrate and octyl acetate of 85.40 and 91.38 ppm, respectively). Low toxicity was registered for both EO and single components against human embryonic kidney (HEK293) cells. Overall, the H. persicum EO, hexyl butyrate, and octyl acetate could be further considered for larvicide development.

Improvement of Bioactive Polyphenol Accumulation in Callus of Salvia atropatana Bunge.

Callus cultures of the Iranian medicinal plant Salvia atropatana were initiated from three-week-old seedlings on Murashige and Skoog (MS) medium supplemented with α-naphthaleneacetic acid (NAA) and various cytokinins. Although all tested hormonal variants of the medium and explant enabled callus induction, the most promising growth was noted for N-(2-chloro-4-pyridyl)-N'-phenylurea (CPPU)-induced calli. Three lines obtained on this medium (cotyledon line-CL, hypocotyl line-HL, and root line-RL) were preselected for further studies. Phenolic compounds in the callus tissues were identified using UPLC-MS (ultra-performance liquid chromatography-mass spectrometry) and quantified with HPLC (high-performance liquid chromatography). All lines exhibited intensive growth and contained twelve phenolic acid derivatives, with rosmarinic acid predominating. The cotyledon-derived callus line displayed the highest growth index values and polyphenol content; this was exposed to different light-emitting diodes (LED) for improving biomass accumulation and secondary metabolite yield. Under LED treatments, all callus lines exhibited enhanced RA and total phenolic content compared to fluorescent light, with the highest levels observed for white (48.5-50.2 mg/g dry weight) and blue (51.4-53.9 mg/g dry weight) LEDs. The selected callus demonstrated strong antioxidant potential in vitro based on the 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) tests. Our findings confirm that the S. atropatana callus system is suitable for enhanced rosmarinic acid production; the selected optimized culture provide high-quality plant-derived products.

Validation of the traditional antimicrobial use of the Iranian medicinal plant Thymus daenensis Celak through phytochemical characterization of its bioactive constituents

Thymus daenensis Celak (Lamiaceae family), known as denaian thyme, is an Iranian endemic plant, commonly used for its carminative, expectorant, antibacterial and antifungal properties. Previous studies report the chemical profile of the essential oil of T. daenensis aerial parts, but little is known about its non volatile constituents. Herein, phytochemical and biological investigation of the polar extract of T. daenensis aerial parts to provide further insight into traditional use of this plant has been accomplished. High-performance liquid chromatography coupled to linear ion-trap and orbitrap high-resolution mass spectrometry (LC-ESI/LTQOrbitrap/MS/MS) analysis of MeOH extract was performed to guide the isolation of specialized metabolites and successive characterization by NMR analysis. MeOH extract displayed antioxidant activity evaluated by DPPH (EC50 = 48.99 ± 1.47 μg/mL) and TEAC assay (1.37 mg/mL). Successively, the biofilm inhibitory activity of extract and isolated compounds against mature biofilms of Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Listeria monocytogenes, and Staphylococcus aureus, and their influence on the metabolism of sessile bacterial cells were evaluated. Two previously unreported thymol derivatives have been identified. The biofilm inhibitory activity of isolated compounds highlighted a promising antimicrobial action for the tested compounds. In particular, vanillic acid, (3S,5R,6R,7E,9S)-megastigm-7-ene-3,5,6,9-tetrol 3-O-β-D-glucopyranoside, thymoquinol-2-O-β-D-glucopyranoside and the never reported compound daenol resulted capable of exerting inhibitory activity vs all pathogenic strains. All compounds tested at a concentration of 10 μg/mL inhibited the metabolism of the sessile cells of E. coli at a percentage ranging between 37.7% and 77.39%, and of L.monocytogenes at a percentage ranging between 21.79% and 71.17%.

Cytotoxic and apoptotic effects of Ferula gummosa Boiss: extract on human breast adenocarcinoma cell line.

Ferula gummosa Boiss. is a well-known and valuable medicinal plant in Iran. Research has shown that this plant has several pharmacological properties, including anti-bacterial, anti-cancer and etc. In the present study, we investigated the cytotoxic properties of F. gummosa Boiss. extract in MCF-7 breast adenocarcinoma cells. The cytotoxicity and pro-apoptotic properties of the extract were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test and propidium iodide (PI) stained cells, respectively. Apoptosis and necrosis were evaluated by annexin V-PI staining. The levels of reactive oxygen species (ROS),malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD) was determined to evaluate oxidative stress. The cell migration and the gene expression were assessed by scratch assay and quantitative real-time polymerase chain reaction (q-RT-PCR), respectively. The extract of F. gummosa decreased the viability and cell cycle progression of MCF-7 cells by inducing apoptosis and necrosis, increasing ROS and MDA levels, and decreasing GSH levels and SOD activity. It also lowered the cells' migration capability by enhancing p53 mRNA levels and reducing MMP-9 mRNA expression. F. gummosa exhibited pro-apoptotic, anti-proliferative, and anti-metastatic effects on MCF-7 cells. It is therefore recommended that detailed future research be done on different parts of the plant or its secondary metabolites to find anti-cancer lead compounds.

In vitro antimicrobial and antibiofilm screening of eighteen Iranian medicinal plants

BackgroundNatural products are one of the best candidates for controlling drug-resistant pathogens, the advantages of which include low production costs and low side effects. In this study, as potential antimicrobials, the anti-bacterial and antibiofilm activities of several Iranian native medicinal plants were screened.MethodsThe antibacterial/antifungal and anti-biofilm activities of 18 medicinal plants including Reseda lutea L., Nepeta sintenisii Bunge., Stachys turcomanica Trautv., Stachys lavandulifolia Vahl, Diarthron antoninae (Pobed.) Kit Tan., Ziziphora clinopodioides Lam., Euphorbia kopetdaghi Prokh, Euphorbia serpens Kunth., Hymenocrater calycinus Benth., Scutellaria pinnatifida A.Ham., Viola tricolor L., Hypericum helianthemoides (Spach) Boiss., Hypericum scabrum L., Convolvulus lineatus L., Scabiosa rotata M.Bieb Greuter & Burdet, Delphinium semibarbatum Bien. Ex Boiss., Glycyrrhiza triphylla Fisch. & C.A.Mey., and Ziziphus jujuba Mill., against two Gram-positive bacteria, Staphylococcus aureus, Bacillus cereus, as well as two Gram-negative bacteria, Pseudomonas aeruginosa, Escherichia coli; and Candida albicans as a fungal strain, were evaluated. The minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) values of the extracts against tested microorganisms were reported and we investigated their effect on the biofilm inhibition of Pseudomonas aeruginosa PAO1, Staphylococcus epidermis, Staphylococcus aureus and Streptococcus mutans. In addition, the effect of the extracts on the eradication of the biofilms of these bacteria was evaluated.ResultsIn this study, H. scabrum was found to exhibit potentially significant activity against Gram-positive bacteria with the MIC range of 6.25–25 µg/mL. This extract also showed a significant effect on inhibiting the biofilm of S. aureus, S. mutans, and S. epidermidis and eradicating the biofilm of S. epidermidis DSMZ 3270. In addition, Hymenocrater calycinus root extract had moderate antibacterial activity against B. cereus with the MIC and MBC 62.5 µg/mL, respectively.ConclusionsThe results of this study showed that the root extracts of two plants, Hypericum scabrum and Hymenocrater calycinus, had antimicrobial and anti-biofilm effects. Based on the observed anti-biofilm effects, these two plants may be considered in future studies to find responsible antimicrobial compounds.

Catechol-type siderophore producer Streptomyces spp. with quorum-quenching activity isolated from wild medicinal plants rhizosphere

Traditional medicinal plants represent a unique source for the isolation of Streptomycetes and antimicrobial compounds. Antimicrobial activity of Streptomyces isolates collected from the rhizosphere of different native medicinal plants in Iran, was investigated against Gram-positive and Gram-negative bacteria. Multi-omics analysis was performed to identify active compounds corresponding to the observed bioactivities. Since cell-to-cell communication mediated by N-acyl homoserine lactones (AHLs) is important for the virulence of Gram-negative pathogenic bacteria, quorum quenching activity of the isolates and their ability to degrade C6-HSL and C8-HSL were tested. Two highly related Streptomyces isolates derived from the Helichrysum rubicundum and Rumex acetosa rhizosphere, respectively, exhibited bioactivity against pathogenic indicator bacteria. Metabolite analysis of extracts obtained from liquid cultures of both isolates revealed the production of the catechol-peptide siderophores streptobactin and tribenarthin. Proteomic analysis confirmed the presence of proteins encoded by the streptobactin and lidamycin biosynthetic gene clusters. Quorum quenching activity of the strains and AHL-degrading enzyme production was confirmed using a GFP producing biosensor and LC-MS analysis. This study demonstrates catecholate siderophore production and QQ potential of two Streptomyces isolates from medicinal plants rhizosphere from Mishan Plain, Hamedan Province, Iran using metabolomics and proteomics analysis. Production of streptobactin by terrestrial Streptomyces sp. strains was confirmed for the first time.

The Hepatoprotective Possessions of Specific Iranian Medicinal Plants

The Hepatoprotective Possessions of Specific Iranian Medicinal Plants

Effect of prechilling and nano-selenium on seed germination of Dorema ammoniacum

The aim of this research was to investigate the effectiveness of nano-selenium (nano-Se) on germination properties of vasha (Dorema ammoniacum), an endangered medicinal plant in Iran. The experiment was conducted in a completely randomised two-factor, experiment with five concentrations (0, 5, 10, 20, and 40 mg L –1) of nano-Se and seed prechilling treatment (4°C). All the germination characteristics, including percentage germination, mean germination and vigour indices were improved by nano-Se treatments (p <; 0.05). Among the treatments, the combination of prechilling and 20 mg L –1 nano-Se conditioning was the best treatment, both in improving germination and the growth characteristics of seedlings. It can be concluded that pretreatment of seeds with nano-Se and prechilling can be used for promoting germination of D. ammoniacum seeds.

Bibliometric and subject analysis of articles in the ethnobotanical field of Iranian medicinal plants (1999 – 2022)

Bibliometric and subject analysis of articles in the ethnobotanical field of Iranian medicinal plants (1999 – 2022)

Scrophularia amplexicaulis increases anti-cancer potential of doxorubicin in gastric cancer cells.

Increased drug resistance has reduced efficiency of chemotherapic drugs such as Doxorubicin (Dox). Scrophularia amplexicaulis (Scr) is one of the most important medicinal plants in Iran that has anti-cancer activity. The aim of this study was to investigate a novel approach to enhance therapeutic efficacy of Dox (as a chemotherapeutic agent) by co-administration of Scr (as a bioactive herbal compound) in gastric cancer treatment. In the present study, effects of Dox, Scr, and their combinations (Scr-Dox) were evaluated on viability and proliferation of two gastric cancer cell lines (AGS and MKN28). Moreover, morphological changes, invasion, migration, colony formation, and apoptosis rate in the treated cancer cells were evaluated. Expression of BAX, BCL2, SAMC, SURVIVIN, CASP9, P53, MMP9, and MMP2 in the treated cancer cells and untreated controls were evaluated by Real-Time PCR method. Treatments of cancer cells by Scr, Dox, and Scr-Dox significantly decreased proliferation, invasion, migration, and colony formation of gastric cancer cells. Treatments of cancer cells by Scr, Dox, and Scr-Dox significantly increased apoptosis rate as well as decreased cells mobility through modification of apoptosis- and metastasis-related genes expression. However, anti-cancer activity of Scr-Dox combination was significantly more than Scr and Dox treatments alone. In general, we demonstrated that Scr-Dox combination therapy exerts more profound anti-cancer effects on AGS and MKN28 cell lines than Scr and Dox monotherapy.

Intraspecific karyomorphological and genome size variations of in vitro embryo derived Iranian endemic Asafoetida (Ferula assa-foetida L., Apiaceae)

Asafoetida (Ferula assa-foetida L.) is one of the endemic medicinal plants in Iran. Analysis of karyomorphology and 2Cx DNA measurements (monoploid genome size) of 18 Iranian endemic Ferula assa-foetida populations were performed. The in vitro embryo-derived root tips were examined for karyological studies, via technique of squash and stain with 2% (w/v) aceto-orcein. Seeds of the Ferula samples and leaves of Solanum lycopersicum as standard reference (2C DNA = 1.96 pg) were stained with propidium iodide (PI), using flow cytometric (FCM) technique. All the studied populations were diploids (2n = 2x = 22) with mean chromosome length (CL) of 3.95 μm, varied from 3.05 μm (P7) to 4.94 μm (P18). The mean total chromosome volume (TCV) was 0.98 μm3, ranged from 0.47 μm3 (P7) to 1.57 μm3 (P3). Two-typed chromosomes (“m”, “sm”) formed three classes of karyotype formula. Karyotypes were mostly symmetrical and fell in 1A and 2A Stebbins category. The monoploid genome size of Iranian endemic Ferula assa-foetida populations is being stated for the first time; its mean value was 4.51 pg, ranged from 4.09 (P4) to 4.69 pg (P16). Intraspecific karyomorphological and genome size variations were clearly confirmed in studied Ferula assa-foetida.

Screening of some Iranian medicinal plants for anti-tuberculosis, anti-bacterial, and cytotoxic activities

Screening of some Iranian medicinal plants for anti-tuberculosis, anti-bacterial, and cytotoxic activities

Scanning electron microscopy, biochemical and enzymatic studies to evaluate hydro-priming and cold plasma treatment effects on the germination of Salvia leriifolia Benth. seeds.

Finding a suitable method to increase seed germination rates of medicinal plants is critical to saving them from extinction. The effects of cold plasma (CP) treatments (using surface power densities of 80 and 100 W, with exposure times of 0, 120, 180, and 240 s) and incorporating hydropriming (carried out for 24 and 2h on normal and uncovered seeds, respectively) to enhance the seed germination of Salvia leriifolia Benth a native endangered Iranian medicinal plant, were evaluated in this study. Scanning electron microscopy (SEM) images identified more destroyed mesh-like structures in hydro-primed and uncovered seeds than in normal and dry seeds. In comparison to the control, and other treatments, employing 100 W of CP for 240 s produced the maximum germination percentage and rate, as well as a seedling vigor of I and II in hydro-primed and uncovered seeds. The levels of α-amylase activity increased when the power and exposure times of CP were increased. The uncovering and hydropriming of S. leriifolia seeds resulted in increased enzyme activity. Malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents were enhanced by increasing the power and exposure time of CP, especially in uncovered and hydro-primed seeds. The activity of antioxidant enzymes, including catalase (CAT) and superoxide dismutase (SOD), was correlated to changes in MDA and H2O2 levels. Finally, direct contact of CP with uncovered seeds in a short exposure time can improve the germination of S. leriifolia seeds via microscopic etching and activation of enzymes.

Natural Products from Leaves of the Ancient Iranian Medicinal Plant Echium amoenum Fisch. & C. A. Mey.

For several millennia, leaves of Echium amoenum Fisch. & C. A. Mey., an important Iranian medicinal plant with nutritional value as nutraceutical, have been used as tea for the treatment of several conditions, including inflammation. The nutritional value of intake of E. amoenum tea has mainly been correlated to its rich content of mainly water-soluble antioxidants. Although the entire plant is utilized, only natural products of the flowers have previously been thoroughly investigated. The rare natural products bis(3-(3,4-dihydroxyphenyl)-1-methoxy-1-oxopropan-2-yl)-1-(3,4-dihydroxyphenyl)-6,7-dihydroxy-1,2-dihydronaphthalene-2,3-dicarboxylate, 4-Oxy-(E)-caffeoyl-2,3-dihydroxybutanoic acid methyl ester and 4-Oxy-(Z)-caffeoyl-2,3-dihydroxybutanoic acid methyl ester, in addition to the widely distributed compounds rosmarinic acid methyl ester and (E)-caffeic acid, were purified and characterized from leaves of Echium amoenum. The structures were determined by a combination of several 2D NMR spectroscopic techniques, circular dichroism spectroscopy and high-resolution mass spectrometry. The fact that bis(3-(3,4-dihydroxyphenyl)-1-methoxy-1-oxopropan-2-yl)-1-(3,4-dihydroxyphenyl)-6,7-dihydroxy-1,2-dihydronaphthalene-2,3-dicarboxylate belongs to a rare group of natural products which have previously been patented for their significant anti-inflammatory activity may rationalize the traditional treatment of inflammations with E. amoenum.

Evaluation of the total antioxidant capacity of <em>Oliveria decumbens</em> and <em>Capparis spinosa</em>

The anti-oxidative potential of extracts from two Iranian medicinal plants, Oliveria decumbens and Capparis spinosa, is assessed in this study. The Oliveria genus is part of the Apiaceae family and contains only one species, O. decumbensVent. Capers are either wild or cultivated plants that are found in many tropical and subtropical countries. Oliveria decumbens and Capparis spinosa aerial parts were dried and ground. The herbal solution was then created by combining herbal powder and methanol. Finally, the total antioxidant capacity of the plants was determined using the Ferric Iron Reducing Antioxidant Power (FRAP) assay and the ELISA reader at 570 nm. The total antioxidant capacities of O. decumbens, C. spinosa fruit, and C. spinosa leaves, respectively, were 3.82 mmol Fe2+/L, 1.96 mmol Fe2+/L, and 1.65 mmol Fe2+/L. These plants' essential oils are secondary metabolites that are widely used in the food, pharmaceutical, and health industries as antioxidant and antimicrobial compounds. In general, the traditional Iranian edible plants studied are good sources of natural antioxidant compounds.

A Review on Wound Healing with Iranian Medicinal Plants and Microbial Flora in Veterinary Medicine

A Review on Wound Healing with Iranian Medicinal Plants and Microbial Flora in Veterinary Medicine

Native Iranian Medicinal Plants with Anti-Vaginal Infection Properties: A Systematic Review.

The use of herbs has recently been considered an effective method in the treatment of infections. The purpose of this study was to introduce Iranian medicinal plants with an effect on vaginal infections. In our systematic review study, keywords including 'medicinal plants', 'Iranian medicinal plants', 'vaginal infection', 'essential oils', and 'extracts' were searched in PubMed, Web of Science Direct, Scopus, Scientific Information Database, and Google Scholar. This study focused on the articles published from 2000 to 2019. According to our investigation, Stachys lavandulifolia, Thymus vulgaris L., Origanum vulgare L., Allium jesdianum, Cichorium intybus, Trigonella foenum-graecum, Azadirachta Indica, Stachys lavandulifolia, Coriandrum sativum L., Rosmarinus officinalis, Ferula gummosa L., Origanum syriacum, Eucalyptus globulus, Myrtus communis, Zingiber officinale, Punica granatum, Vitex agnus-castus, Mentha suaveolens, Quercus Brantii Lindl, Zataria multiflora, Berberis vulgaris, Thymus vulgaris L., Echinophora platyloba, Stachys lavandulifolia, Viola odorata, Menthe piperita, Eucalyptus camaldulensis, Ziziphus mauritiana, Quercus infectoria, Hypericum perforatum, Glycyrrhiza glabra L., Calendula officinalis, Origanum vulgare, Ziziphus nummularia, Satureja Bachtiarica, Foeniculum vulgare, Nigella sativa, Anethum graveolens, Salvia officinalis, and Calendula officinalis are used to treat vaginal infections. Therefore, native plants of Iran have been reported to have antimicrobial properties for the treatment of vaginal infections and can be used as a suitable and effective alternative to antibiotics in future research.

Inhibitory Effect of Nepeta Crispa L. and Nepeta Cataria Extracts on Candida Albicans Growth: An In-vitro Study

Background and Objectives Considering the existence of many medicinal plants in Iran and the high prevalence of oral candidiasis, the present study aims to evaluate the inhibitory effects of Nepeta crispa L. and Nepeta cataria extracts on Candida albicans growth. Subjects and Methods After preparing the extracts of two plants, their minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC) for C albicans were measured by broth microdilution method. The inhibitory effect of the extracts was evaluated by the disk diffusion method and measuring the diameter of inhibition zone. Data were analyzed in SPSS software, version 22. The significant level was set at 0.05. Results The MIC of N. crispa L. and N. cataria extracts was 1.1 and 2.4 μg/mL, respectively. Their MLC was 4 and 6 μg/mL, respectively. The highest inhibition zone diameter was for nystatin (21.31 mm), while the lowest diameter was for N. cataria extract (11.60 mm). No. significant difference was observed in the inhibition zone diameter between nystatin and N. crispa L. extract (P>0.05). Conclusion The inhibitory effect of N. crispa L. extract on the candida albicans growth is not significantly different from the effect of nystatin. Although N. cataria extract has inhibitory effect, its effect is significantly lower than the effect of nystatin.

In Vitro Antimalarial Activity and Phytochemical Analysis of Aerial Parts of Artemisia fragrans Willd.

Background: Malaria is one of the most momentous transmittable diseases globally. Medicinal herbs like Artemisia species might be possible sources of new, effective, and cheap antiplasmodial products, making up the leading molecules to investigate new antimalarial drugs. The Artemisia genus, which belongs to the Asteraceae family, is a widely distributed medicinal plant in Iran. Methods: In this study, the antimalarial activities of essential oil, different extracts, and vacuum liquid chromatography (VLC) fractions of A. fragrans Willd. were examined by a cell-free β-hematin formation assay. The aerial parts of A. fragrans were extracted by a Soxhlet extractor, and essential oil was obtained by a Clevenger apparatus. Then, GC-MS analysis was used to identify volatile compounds of essential oil and the 100% VLC fraction of chloroform. Results: Among the extracts, chloroform extract illustrated considerable antimalarial activity compared to the control (P < 0.001), with the IC50 value of 1.22 ± 0.05 mg/mL. Among the fractions, 100% VLC fraction of chloroform extract illustrated potent antimalarial effects compared to the control (P < 0.001). The volatile oil demonstrated moderate antimalarial effects (P < 0.001) compared with the control. Besides, GC-MS determined that sesquiterpenes in the 100% ethyl acetate fraction of the chloroform extract and oxygenated monoterpenes in the essential oil might be responsible for the potent antimalarial activity of this plant. Conclusions: The 100% ethyl acetate fraction of chloroform extract along with the essential oil of A. fragrans indicated potent and moderate activity, possibly due to sesquiterpenes and oxygenated monoterpenes, respectively.

In vitro evaluation of the amount of flavonoids, flavanols, and total phenolic compounds in 10 medicinal plants used in Iran; focus on diabetes and kidney diseases

Introduction: Free radicals and reactive oxygen species (ROS) as oxidants are important concerns in medicine due to their adversative effects and risk of carcinogenicity. Antioxidants can neutralize free radicals and ROS thus possesses protective effect on the body. Plants are rich sources of natural antioxidants and phenolic compounds are among the most important antioxidants in the plants; therefore, many studies have investigated their protective effects. Objectives: This study was conducted to evaluate and compare the number of flavonoids, flavonols, and total phenolic compounds in 10 commonly used medicinal plants in Iran. Materials and Methods: Extraction was performed using 70% ethanol solvent. The extracts were concentrated by rotary evaporator at 40°C. Folin-Ciocalteu method was used to measure the amount of phenol. The amount of flavonol was measured using 2% aluminum chloride and 5% sodium acetate. In addition, to determine the flavonoid compounds, 2% aluminum chloride and 5% potassium acetate was used. Results: The outcomes of this study disclosed that the studied plants had reasonably high quantities of flavonoid and phenolic compounds. The uppermost amount of phenolic compounds was detected in the myrtle plant (62.7 mg/g), and the lowermost amounts were detected in fig leaves and lemon balm. Moreover, the highest amount of flavonoid compounds was detected in ginger, myrobalan, and myrtle; the measured amounts of flavonoid compounds in the mentioned plants were 42.49, 32.15, and 34.38 mg/g, respectively. The highest amount of phenolic compound was detected in hydroalcoholic extract of barberry. Conclusion: Hydroalcoholic extract of ginger, barberry, fig leaves, rhubarb, myrobalan, walnut diaphragm, pomegranate peel, lemon balm, cardamom, and myrtle had great quantities of phenolic, flavonol, and flavonoid compounds. As a result, these herbal plants can be considered and used as an important source of natural antioxidants.