This study evaluated the effects of dietary supplementation of Iranian commercial multi-strain probiotic (IMSP) or different levels of Pediococcus acidilactici (PA), isolated from Iranian camel milk, on some biological parameters in male Ross broilers. A total of 240 1-d-old chicks were divided into four groups as follows: (1) Control-Unsupplemented, (2) supplemented with MSP, (3)106 CFU PA g−1diet, (4)108 CFU PA g−1diet (4). Groups 2 and 3 exhibited a higher total growth rate and feed conversion ratio, compared to the control and group 4 (p < .05). Group 3 showed a larger villus length/crypt-depth compared to the control (p < .05). Groups 2 and 4 displayed lower and larger count of jejunal Gram (−) bacteria and Lactobacillus spp. compared to the control, respectively (p < .05). The control group showed the lowest levels of total serum protein (p < .05). Group 3 had lower blood triglycerides compared to the control and group 2 (p < .05). These results indicated that the IMSP and 106 CFU PA g−1diet can improve growth and FCR and can be used in the poultry industry of Iran. HIGHLIGHTS Supplementation diet with native multi-strain probiotic is useful. Pediococcus acidilactici isolated from Iranian camel milk can improve broilers performance and reduce blood triglycerides.