Effect Of Ionic Strength Research Articles

Recent Advances in Simulation Studies on the Protein Corona

When flowing through the blood stream, drug carriers such as nanoparticles encounter hundreds of plasma proteins, forming a protein layer on the nanoparticle surface, known as the “protein corona”. Since the protein corona influences the size, shape, and surface properties of nanoparticles, it can modulate their circulating lifetime, cytotoxicity, and targeting efficiency. Therefore, understanding the mechanism of protein corona formation at the atomic scale is crucial, which has become possible due to advances in computer power and simulation methodologies. This review covers the following topics: (1) the structure, dynamics, and composition of protein corona on nanoparticles; (2) the effects of protein concentration and ionic strength on protein corona formation; (3) the effects of particle size, morphology, and surface properties on corona formation; (4) the interactions among lipids, membranes, and nanoparticles with the protein corona. For each topic, mesoscale, coarse-grained, and all-atom molecular dynamics simulations since 2020 are discussed. These simulations not only successfully reproduce experimental observations but also provide physical insights into the protein corona formation. In particular, these simulation findings can be applied to manipulate the formation of a protein corona that can target specific cells, aiding in the rational design of nanomedicines for drug delivery applications.

Charge Mediated Changes to the Intrinsic Viscosity of Biopolymer Systems

A theoretical approach is presented to quantify the effect of ionic strength on the swelling and shrinkage of the hydrodynamic coil size of a generic biopolymer. This was conducted in view of extraction methods that often utilize acids and alkali combinations and, therefore, invariably impact the levels of salt found in commercially available biopolymers. This approach is supplemented by intrinsic viscosity measurements for the purpose of validation across a variety of biopolymer architectures, type of functionalization, as well as the quoted molar mass. By accurately capturing the magnitude of change in the coil size, it is discussed how a biopolymer coil size is far more sensitive to changes in the ionic strength than it is to the molar mass (or contour length) itself. In turn, it is highlighted why the current characterization strategies that make use of weight-averaged molar mass are prone to errors and cannot be used to establish structure—property relationships for biopolymers. As an alternative, the scope of developing an accurate understanding of coil sizes due to changes in the “soft” interactions is proposed, and it is recommended to use the coil size itself to highlight the underlying structure—property relationships.

Activated carbons prepared from stump wood of various tree species by chemical activation and their application for water purification

AbstractActivated carbons (ACs) were produced from stump wood of different tree species, such as pine, bearded birch, and American black cherry using chemical activation with KOH and NaOH. The activated carbons were characterized and evaluated as adsorbents for eliminating bisphenol A (BPA) from aqueous solutions. The kinetics of adsorption and equilibrium adsorption, as well as the impact of solution pH and ionic strength, were examined. The kinetics were analyzed using the pseudo-first-order, pseudo-second-order, intra-particle diffusion, and Boyd kinetic models. The findings suggest that the adsorption kinetics followed the pseudo-second-order model. Additionally, the film diffusion was found to be the rate-determining step for the adsorption of BPA on all of the activated carbons. The data for adsorption equilibrium were tested using the Langmuir, Freundlich, and Sips equations, with results indicating that the Langmuir model was the most applicable. The capacity of activated carbons to adsorb BPA was dependent on their surface area. Higher BET surface areas resulted in increased adsorption. The birch-derived AC activated by NaOH had a monolayer adsorption capacity of 1.980 mmol/g, while the AC from black cherry activated with KOH had 2.195 mmol/g. The adsorption of BPA was pH-dependent, and no effect of ionic strength was observed. The activated carbons had very high adsorption capacities, indicating that stump wood is an excellent precursor for the production of highly effective adsorbents.

Encapsulation of β-Galactosidase into Polyallylamine/Polystyrene Sulphonate Polyelectrolyte Microcapsules.

More than half of the global population is unable to consume dairy products due to lactose intolerance (hypolactasia). Current enzyme replacement therapy methods are insufficiently effective as a therapeutic approach to treating lactose intolerance. The encapsulation of β-galactosidase in polyelectrolyte microcapsules by using the layer-by-layer method could be a possible solution to this problem. In this study, adsorption and co-precipitation methods were employed for encapsulating β-galactosidase in polyelectrolyte microcapsules composed of (polyallylamine /polystyrene sulphonate)₃. As a result, the co-precipitation method was chosen for β-galactosidase encapsulation. The adsorption method permits to encapsulate six times less enzyme compared with the co-precipitation method; the β-galactosidase encapsulated via the co-precipitation method released no more than 20% of the initially encapsulated enzyme in pH 2 or 1 M NaCl solutions. In contrast, when using the sorption method, about 100% of the initially encapsulated enzyme was released from the microcapsules under the conditions described above. The co-precipitation method effectively prevents the complete loss of enzyme activity after 2 h of incubation in a solution with pH 2 while also alleviating the adverse effects of ionic strength. Consequently, the encapsulated form of β-galactosidase shows promise as a potential therapeutic agent for enzyme replacement therapy in the treatment of hypolactasia.

Preparation of chitosan/cellulose nanofibril composite aerogel and its adsorption performance for Cu(II)-MO binary pollutant

Heavy metals and organic dyes commonly coexist in water, which pose a serious threat to human health. Herein, a functional aerogel for adsorption of Cu(II)-methyl orange binary-polluted system was prepared. Cellulose nanofibril (CNF) was prepared by 2,2,6,6-tetramethylpiperidinyloxy (TEMPO)-NaBr-NaClO system using abandoned pineapple leaves as the main raw material, and chitosan/cellulose nanofibril (CS/CNF) composite aerogel was constructed by sol-gel method combined with freeze-drying. The structure of composite aerogel was characterized by XRD, FTIR, TG and SEM. The fabricated aerogels were ultra-lightweight and exhibited a highly porous 3D network structure. The effects of adsorbent dosage, ionic strength, solution pH, adsorbent concentration, adsorption time, and temperature on the adsorption of Cu(II) and methyl orange (MO) by composite aerogel were studied. The adsorption of composite aerogel towards mono-polluted of Cu(II) and MO reached equilibrium after 100 min with a maximum adsorption capacity of 116.69 and 295.86 mg/g, respectively. The adsorption of Cu(II) and MO by CS/CNF aerogel was mainly achieved through electrostatic attraction, metal chelation and hydrogen bonding interactions. More importantly, the adsorption of Cu(II) by CS/CNF aerogel has an inhibitory effect on its adsorption of MO in Cu(II)-MO binary-polluted system.

Water-Polymer Slide Electrification in Polyethylene Films Coated with Amphiphilic Compounds and Its Connection to Surface Properties Dependent on Water-Polymer Interactions.

Slide electrification experiments were performed on low-density polyethylene films (PE) and PE sprayed with five amphiphilic compounds, including antistatic and slip additives. Drops of aqueous solutions were delivered on the films and after sliding spontaneously acquired a net electrical charge (Qdrop), which is dependent on the pH and ionic strength. The slide electrification was detected in pristine PE films and those with five additives. An acid-base equilibrium model, based on the adsorption of hydroxides and protons on surface sites, accounted for the dependence of Qdrop on pH, allowing recovery of the acid-base equilibrium constants and the density of adsorption sites. The model was modified to account for ionic strength effects through activity factors. The surface conductivity, wettability, and friction coefficients were strongly modified by the additives. However, the observed trends are different from those observed in slide electrification, which better correlated with zeta potential determinations. This suggests that the interaction mechanisms among surface water, the considered additives, and the polymer, which are involved in slide electrification and the generation of zeta potential, are different from those associated with other surface processes involving surface water. Although additives are required for changing surface resistivity, friction coefficients, and wettability, the generation of sliding electrical charges in polyethylene is a spontaneous and highly effective process. For one specific additive, a simultaneous decrease in friction coefficients, zeta potential, and Qdrop was observed, assigned to the blockade of hydroxide adsorption sites and water repulsion by the compound.

Solubility-controlled early age hydration of carbonate-activated slag: Underlying mechanisms and acceleration via seeding

The low environmental impacts and good performance of carbonate-activated binders have attracted substantial interest, while their intrinsically delayed reaction and prolonged setting times constrain widespread implementation. This study aims to investigate the early-age reaction kinetics of carbonate-activated binders, elucidate the underlying mechanisms and propose controlling methods. Results reveal that hydrotalcite and C-A-S-H gel precursors rapidly reach supersaturation within hours, yet precipitation of these phases remains absent. This phenomenon, coupled with high Si/Ca ratios in the pore solution, exhibits characteristics that may contribute to the observed reaction delays. A prolonged induction period and lethargic strength development are observed owing to the lack of strength-giving phases. The hydrotalcite and C-A-S-H concentrations surpass their theoretical solubility thresholds due to rising ionic strength and salt-in effects. The addition of a pH-neutral layered double hydroxide salt accelerates the reaction, confirming seeding impacts control pore solution saturation limits, constituting the underlying accelerator mechanism.

Analysis of interactions between pharmaceuticals and humic acid: Characterization using entrapment and high-performance affinity microcolumns

The presence of pharmaceuticals as microcontaminants in the environment has become of particular concern given the growing increase in water reuse and recycling to promote global sustainability of this resource. Pharmaceuticals can often undergo reversible interactions with soluble dissolved organic material such as humic acid, which may be an important factor in determining the bioavailability and effects of these compounds in the environment. In this study, high-performance affinity microcolumns containing non-covalently entrapped and immobilized humic acid are used to examine the binding strength and interactions of this agent for tetracycline, carbamazepine, ciprofloxacin, and norfloxacin, all common pharmaceutical microcontaminants known to bind humic acid. The binding constants, as measured with Aldrich humic acid, have good agreement with values reported in the literature. In addition, the effects of temperature, ionic strength, and pH on these interactions are examined with the humic acid microcolumns. This technique makes it possible to determine the relative importance of electrostatic interactions vs non-polar interactions or hydrogen bonding on these binding processes. This study illustrates how affinity microcolumns can be used to screen and uniformly quantify binding by pharmaceuticals with humic acid, as well as to study the mechanisms of these interactions, with this information often being acquired in minutes and with small amounts of binding agent (∼10 mg per microcolumn, which could be used over 200–300 experiments). Use of entrapment and affinity microcolumns can support similar research for a wide range of other microcontaminants with humic acid or alternative binding agents found in water and the environment.

Co-doped NH2-UiO-66 for fast and effective adsorption of oxytetracycline: Kinetics, thermodynamics and mechanism

Novel type of micro mesoporous materials Co-doped NH2-UiO-66 had been successfully prepared. The properties of the material were understood through a series of characterization. When the dosage of Zr:Co is 2:1, the modified material showed specific surface area of 840.02 m2∙g−1, which is larger than the material without Co. Especially, the material was transformed from microporous NH2-UiO-66 to micro-mesoporous Co-doped NH2-UiO-66 through the adjustment of metal Co. The Co-doped nanocomposite was then applied to the adsorption of oxytetracycline. The effects of initial concentration, pH, humic acid and ionic strength on the adsorption were investigated. It was found that 50 mg∙L−1 oxytetracycline can be effectively adsorbed by Co-doped NH2-UiO-66 in pH 7. Further research showed that adsorption was pseudo-second-order kinetics and fitted Freundlich model with the maximum adsorption capacity 223.7 mg∙g−1. In addition, the adsorption equilibrium time was only 2 h, which is shorter than the adsorption time of most adsorbents. The mechanism of pore adsorption, π-π interaction, hydrogen bonds and electrostatic interactions was deeply discussed. In conclusion, Co-doped NH2-UiO-66 was a fast and effective absorbent for the removal of oxytetracycline in aqueous solution.

Heavy metal adsorption on yellow loam clay modified by biomass- and biochar-decomposed liquids from Ficus microcarpa

In studing the heavy metal adsorption effect of decomposed liquid (Dl) on yellow loam clay (C), biomass-Dl (BmDl) and biochar-Dl (BcDl) from Ficus microcarpa leaves were used to modify C. The different Dl-modified Cs (Dl-Cs) were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. A batch method was used to study the adsorption kinetics and thermodynamics of Pb(II) and Cd(II), as well as the effects of decomposition time, modification ratio, pH, temperature, and ionic strength on the adsorption process. Results of microscopic morphology indicated that Dl was modified on the C surface, and it changed the surface properties. The adsorption isotherms of Pb(II) and Cd(II) by different Dl-Cs conformed to the Langmuir and Freundlich model, with the maximum adsorption capacity (qm) maintained at 65.64–163.89mmol/kg (BmDl-Cs) and 77.68–235.63mmol/kg (BcDl-Cs). Under the same conditions, the peak value of qm was reached at decomposition time of 14 days and 50% of Dl modification. pH = 6, ionic strength of 0.1mol/L, and 30 ℃ were the optimum conditions for the adsorption of Pb(II) and Cd(II) by different Dl-Cs. Pb(II) and Cd(II) adsorption was a spontaneous, endothermic, and entropy-increasing process, and pseudo-first-order kinetic equation was suitable to characterize adsorption.

Experimental and kinetics of the oxidation of ammonium sulfite by hydrogen peroxide in ammonia flue gas desulfurization

ABSTRACT The oxidation of ammonium sulfite obtained from ammonia-based flue gas desulfurization by hydrogen peroxide in aqueous solutions was studied using a stirred reactor. An experimental study of the kinetic parameters of the oxidation process was investigated in the pH of 4.2–5.2 at 25–50°C. r = k 3 H + HS O 3 − H 2 O 2 is valid at sulfite concentrations of 10−4 mol/L for the pH range 4.2–5.2. At 298K and pH of 5.0, third-order rate constant k 3 was found to be (6.9 ± 0.21)×107 (mol/L)−2s−1. When the concentration of sulfite is 0.01 mol/L, the reaction first-order with respect to sulfite and hydrogen peroxide, and the second-order reaction rate constant at pH of 4.2–5.4 is lg k 2 = − 1.0135 pH + 7.4862 . Activation energy of E a = 32.26 kJ/moL. Kinetic data were compared with previous studies. A semi-empirical formula can express the effect of ionic strength on k 3. The kinetic data of ammonium sulfite oxidation with hydrogen peroxide have potential applications in the wet ammonia desulfurization process.

Bactericide adsorption by purple soil modified by decomposed liquid and humic substance from Bischofia javanica

To investigate the effect of plant decomposition products on bactericide adsorption, purple soil (P) was modified using different ratios of decomposed liquid (Dl) and humic substance (Hs) from Bischofia javanica. Isothermal adsorption characteristics of fenaminosulf and hymexazol by different Dl- and Hs-modified Ps (Dl-P and Hs–P) were studied using the batch method. The effects of pH, ionic strength, and temperature on bactericides adsorption were analyzed. The adsorption process of fenaminosulf and hymexazol on modified Ps was suitable to be described by the Freundlich model. Meanwhile, the adsorption capacity of two bactericides on Hs-Ps was higher than that on Dl-Ps. Acidic conditions and low temperatures were more favorable for the adsorption of fenaminosulf and hymexazol on modified Ps. The adsorption amount of fenaminosulf increased first and then decreased, but that of hymexazol decreased with the increase in ionic strength. The adsorption process of two bactericides was a spontaneous, exothermic entropy-increasing reaction, and the physical adsorption mechanism mainly controlled the adsorption rate. In conclusion, Dl-Ps and Hs-Ps showed potential environmental remediation applications.

Interactions between Hyaluronic Acid and Chitosan by Isothermal Titration Calorimetry: The Effect of Ionic Strength, pH, and Polymer Molecular Weight.

Hyaluronic acid (HA)/chitosan (CHI) complex coacervates have recently gained interest due to the pH-dependent ionization and semiflexibility of the polymers as well as their applicability in tissue engineering. Here, we apply isothermal titration calorimetry (ITC) to understand the apparent thermodynamics of coacervation for HA/CHI as a function of the pH, ionic strength, and chain length. We couple these ITC experiments with the knowledge of the charge states of HA and CHI from potentiometric titration to understand the mechanistic aspects of complex formation. Our data demonstrate that the driving force for the complex coacervation of HA and CHI is entropic in nature and this driving force decreased with increasing ionic strength. We also observed a decrease in the stoichiometry for ion-pairing with increasing ionic strength, which we suggest is a consequence of the changing degree of ionization for HA at higher ionic strengths. An increase in the strength of interactions with pH was hypothesized to also be a result of changes in the degree of ionization of HA, though stronger interactions were observed at the lowest pH tested, likely due to contributions from hydrogen bonding between HA and CHI.

Nano-Hydroxyapatite Modified Tobacco Stalk-Based Biochar for Immobilizing Cd(II): Interfacial Adsorption Behavior and Mechanisms

Biochar, an eco-friendly, porous carbon-rich material, is widely studied for immobilizing heavy metals in contaminated environments. This study prepared tobacco stalks, a typical agricultural waste, into biochar (TSB) modified by hydroxyapatite (HAP) at co-pyrolysis temperatures of 350 °C and 550 °C to explore its Cd(II) adsorption behavior and relevant mechanisms. XRD, SEM–EDS, FTIR, and BET analyses revealed that HAP successfully incorporated onto TSB, enriching the surface oxygen-containing functional groups (P–O and carboxyl), and contributing to the enhancement of the specific surface area from 2.52 (TSB350) and 3.63 m2/g (TSB550) to 14.07 (HAP–TSB350) and 18.36 m2/g (HAP–TSB550). The kinetics of Cd(II) adsorption onto TSB and HAP–TSB is well described by the pseudo-second-order model. Isotherm results revealed that the maximum adsorption capacities of Cd(II) on HAP–TSB350 and HAP–TSB550 were approximately 13.17 and 14.50 mg/g, 2.67 and 9.24 times those of TSB350 and TSB550, respectively. The Cd(II) adsorption amounts on TSBs and HAP–TSBs increased significantly with increasing pH, especially in HAP–TSB550. Ionic strength effects and XPS analysis showed that Cd(II) adsorption onto HAP–TSBs occurred mainly via electrostatic interaction, cation exchange with Ca2+, complexation with P–O and –COOH, and surface precipitation. These findings will provide a modification strategy for the reutilization of tobacco agricultural waste in the remediation of heavy metal contaminated areas.

Lipid digestion profiles of pickering emulsion gels stabilized by β-glucans microgel particles

Pickering emulsion gels (PEGs) stabilized by microgel particles (MPs) have a wide range of applications. However, the extent and mechanisms of lipid digestion in these PEGs are still unclear. In this study, we explored how the concentration of β-glucans MPs and different stabilizers influence the lipid digestion profiles. Our findings revealed a significant role of β-glucans MPs in modulating lipid digestion, with a reduction in lipid digestion rate and extent correlated with an increase in MPs concentration. Notably, compared to droplets stabilized by other agents, emulsions stabilized by β-glucans MPs exhibited the lowest initial release rate (0.81 ± 0.08 FFA%/min) and final degree of digestion (18.96% ± 0.42%) of free fatty acids. Lipid droplets stabilized by β-glucan MPs were able to effectively resist the effects of high ionic strength and complex components in the oral and gastric environments, maintaining their structural integrity and preventing droplet aggregation. Our results indicated that β-glucans MPs are not only effective stabilizers but also provide a novel approach to controlling lipid digestibility.

Carbon Nanomaterials with SOD-like Activity: The Effect of the Ionic Strength.

Electrogenerated hydrophilic carbon (EHC) nanomaterials emerge as a highly attractive option for mimicking the activity of the superoxide dismutase enzyme (SOD) due to their exceptional water solubility and electron-transfer reversibility. Motivated by these properties, the EHC nanomaterials were utilized to assess the effect of ionic strength on the SOD-like activity. Superoxide anion radicals (O2•-) were generated using the hypoxanthine-xanthine oxidase system, with nitro blue tetrazolium chloride serving as the detecting system. A significant boost in the SOD-like activity was found via the addition of an electrolyte to the as-prepared nanomaterial solution. The effect of the electrolyte cation (Na+ and K+), as well as its counterion (Cl-, CH3COO-, and H2PO4-/HPO42-) were analyzed. Based on these studies, a new formulation for the preparation of the carbon-based nanomaterial was established. It was demonstrated that the SOD-like activity follows an enzyme-type catalytic activity rather than the stoichiometric scavenging of the superoxide anion radical. It was concluded that 12.71 µg/mL of the EHC nanomaterial exhibits catalytic activity comparable to 15.46 µg/mL of the native Cu/Zn-SOD enzyme. This study provides a starting point for the development of a new nanotool to fight the oxidative stress associated with pathophysiological conditions where SOD activity is depleted.

Desmodium intortum (Mill.) Urb. Protein Isolate Aggregates as Pickering Stabilizers: Physicochemical Characteristics and Emulsifying Properties.

This work aimed to investigate the feasibility of fabricating Pickering emulsions stabilized by Desmodium intortum protein isolate (DIPI) aggregates. The DIPI aggregates were formed using heat treatment, and the effects of ionic strength and pH on their properties were investigated. The heat-treated protein exposes its hydrophobic groups due to structural damage, resulting in rapid aggregation of the protein into aggregates with a size of 236 nm. The results showed that the aggregates induced by ionic strength had larger particle size and higher surface hydrophobicity and partial wettability. Moreover, this study explored effective strategies for bolstering Pickering emulsion stability through optimized DIPI aggregate concentration (c) and oil fraction (ø). The DIPI Pickering emulsion (DIPIPE) formed at c = 5% and ø = 0.7 was still highly stable after 30 days of storage. As confirmed by laser confocal microscopy, DIPI aggregates could be adsorbed onto the oil-water interface to form a network structure that could trap oil droplets in the network. Collectively, the Pickering emulsion stabilized by DIPI aggregates exhibited excellent stability, which not only deeply utilizes the low-value protein resources in the Desmodium intortum for the first time, but also demonstrates the potential of DIPI for the bio-based field.

Utilization of dragon fruit (Hylocereus undatus) peel-derived biochar for the adsorptive removal of tetracycline from aqueous solution

The peel of Hylocereus undatus was employed in the preparation of biochar and firstly applied for tetracycline removal from aqueous solution. Based on different characterization techniques, the material was found to possess a variety of surface functional groups on a porous structure and a pH point of zero charge (pHpzc) of 9.3. Adsorption of tetracycline (TC) was conducted under varying conditions, revealing significant effects of carbonization temperature, solution pH, adsorbent dose, ionic strength, contact time and initial concentration of TC on the biochar adsorption capacity. Kinetic data on TC adsorption were best described using the Elovich kinetic model, with an initial adsorption rate of 167.3 mg g−1 min−1. Isotherm data on adsorption of the desired biochar showed the best fit with the Temkin isotherm model, followed by the Langmuir model, displaying maximum adsorption capacity at 12.4 mg g−1. The electrostatic interactions between the charged biochar surfaces and certain fractions of TC were proposed as the major mechanism, together with H-bonding, pore-filling effect and π–π interaction. This study demonstrates great potential of H. undatus peel as a starting material to prepare an effective and reusable adsorbent in the removal of TC.

Preparation of multi-modified/carbonized/gelatinized starch and its de-risking effect on Cd(II) and hymexazol in wastewater

In this study, starch (S) was gelatinized and carbonized to prepare carbonized/gelatinized S (CGS) as the research material. Then, peat extract (Pe) and surfactants with different ratios were single- and multi-modified on CGS, respectively, to prepare Pe-modified CGS (Pe-CGS) and multi-modified CGS, respectively. The microscopic morphology of multi-modified CGS was studied using various testing methods. The de-risking effect on Cd(II) and hymexazol in wastewater was investigated, and the effects of temperature, pH, and ionic strength were compared. The spheroidal structure of S was destroyed after carbonization, and Pe and surfactants were modified on the surface and changed the surface properties of CGS. The adsorption processes of Cd(II) and hymexazol were suitable to be described by the Langmuir and Freundlich models, respectively. The maximum adsorption capacities (qm) of Cd(II) and adsorption capacity parameter (k) of hymexazol on different modified CGSs presented the peak value at BS/Pe-CGS. With the increase in the modification ratio of Pe, BS, and SDS, qm and k increased, which showed a high value at 100 % modification. Increases in temperature and pH were beneficial to Cd(II) adsorption but were not conducive to hymexazol adsorption. The adsorption amount decreased for Cd(II) and increased first and then reduced for hymexazol with the rise in ionic strength. The adsorption process exhibited spontaneity, endothermic behavior for Cd(II), exothermic behavior for hymexazol, and an entropy-increasing reaction. The adsorption amount of Cd(II) and hymexazol by multi-modified CGS maintained approximately 81 % of the original sample after three rounds of regeneration.

Effect of pH and sodium ion on the interfacial adsorption abilities and emulsion stability of soy hull polysaccharide

To investigate the effect of ionic strength and pH on the interfacial adsorption capacity and emulsion stability of soy hull polysaccharide (SHP), a series of SHP emulsions with 10% oil content was prepared with 0–0.30 mol/L Na+ at pH 3.0 and 5.0. The results of droplets size, zeta potential, dilatational rheological, and cryo-scanning electron microscopy analyses showed that the droplets of the emulsion at pH 3.0 were smaller and the system was more stable than at pH 5.0, and Na+ effectively shielded the negative charge on the SHP surface, leading to more SHP adsorption to the interface and increasing viscosity; whereas too much Na+ weakened the repulsive forces between droplets, making it easier for the droplets to merge with each other and causing emulsion destabilization. The best emulsification capacity of SHP was achieved at pH 3.0 with 0.10 mol/L Na+, in which 58.62 ± 0.49% SHP would adsorb to the interface, and kept high zeta potential of interface with −30.00 ± 0.27 mV. Meanwhile, the emulsion prepared under this condition had good storage stability and exhibited small droplet size of 2.425 ± 0.004 µm after 30 days. In conclusion, above indicators demonstrated that SHP-stabilized emulsions were influenced by pH and Na+, this provides theoretical and practical support for selecting the most suitable acid-base and ionic environment to prepare food emulsion by SHP, or other anionic polysaccharides.