Cyclotron Resonance Research Articles

Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Applications for Metabolomics

Metabolomics is an interdisciplinary field that aims to study all metabolites < 1500 Da that are ubiquitously found within all organisms. Metabolomics is experiencing exponential growth and commonly relies on high-resolution mass spectrometry (HRMS). Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) is a form of HRMS that is particularly well suited for metabolomics research due to its exceptionally high resolution (105–106) and sensitivity with a mass accuracy in parts per billion (ppb). In this regard, FT-ICR-MS can provide valuable insights into the metabolomics analysis of complex biological systems due to unique capabilities such as the easy separation of isobaric and isomeric species, isotopic fine structure analysis, spatial resolution of metabolites in cells and tissues, and a high confidence (<1 ppm mass error) in metabolite identification. Alternatively, the large and complex data sets, long acquisition times, high cost, and limited access mainly through national mass spectrometry facilities may impede the routine adoption of FT-ICR-MS by metabolomics researchers. This review examines recent applications of FT-ICR-MS metabolomics in the search for clinical and non-human biomarkers; for the analysis of food, beverage, and environmental samples; and for the high-resolution imaging of tissues and other biological samples. We provide recent examples of metabolomics studies that highlight the advantages of FT-ICR-MS for the detailed and reliable characterization of the metabolome. Additionally, we offer some practical considerations for implementing FT-ICR-MS into a research program by providing a list of FT-ICR-MS facilities and by identifying different high-throughput interfaces, varieties of sample types, analysis methods (e.g., van Krevelen diagrams, Kendrick mass defect plot, etc.), and sample preparation and handling protocols used in FT-ICR-MS experiments. Overall, FT-ICR-MS holds great promise as a vital research tool for advancing metabolomics investigations.

Hydrothermal temperature-dependent compositions and copper complexing behaviors of hydrochar-derived dissolved organic matter: Insights from FT-ICR MS and multi-spectroscopic analysis

The copper complexing of dissolved organic matter released from hydrochar (HDOM) affects the former's environmental behavior. In this study, how hydrothermal temperatures (180, 220 and 260 °C) influence the molecular-level constitutions and Cu(Ⅱ) binding features of HDOM were elucidated via fourier transform ion cyclotron resonance mass spectrometry and multi-spectroscopic analysis. The findings demonstrated that the almost HDOM molecules had the traits of lower polarity and higher hydrophobicity. As the hydrothermal temperature increased, the molecules with particularly high relative strength gradually disappeared, average molecular weight, percentages of CHON and aliphatic of HDOM reduced while the percentages of CHO and aromatic compounds increased. In general, the fluorescence quenching of Cu(Ⅱ) weakened as hydrothermal temperature rose and the Cu(Ⅱ) binding stability constants of fluorophores in HDOM were 4.50–5.31. In addition, the Cu(Ⅱ) binding order of fluorophores in HDOM showed temperature heterogeneities, and polysaccharides or aromatic rings of non-fluorescent substances had the fastest responses to Cu(II) binding. Generally, fluorescent components tend to bind Cu(II) at relatively trace concentrations (0–40 µmol/L), whereas non-fluorescent substances tend to the bind Cu(II) at relatively higher concentrations (50–100 µmol/L). This study contributed to the prediction of the potential environmental behavior and risk of Cu(II) at the molecular level after hydrochar application.

Automatic Detection of Quasi-Periodic Emissions from Satellite Observations by Using DETR Method

The ionospheric quasi-periodic wave is a type of typical and common electromagnetic wave phenomenon occurring in extremely low-frequency (ELF) and very low-frequency ranges (VLF). These emissions propagate in a distinct whistler-wave mode, with varying periodic modulations of the wave intensity over time scales from several seconds to a few minutes. We developed an automatic detection model for the QP waves in the ELF band recorded by the China Seismo-Electromagnetic Satellite. Based on the 827 QP wave events, which were collected through visual screening from the electromagnetic field observations, an automatic detection model based on the Transformer architecture was built. This model, comprising 34.27 million parameters, was trained and evaluated. It achieved mean average precision of 92.3% on the validation dataset, operating at a frame rate of 39.3 frames per second. Notably, after incorporating the proton cyclotron frequency constraint, the model displayed promising performance. Its lightweight design facilitates easy deployment on satellite equipment, significantly enhancing the feasibility of on-board detection.

COSMONAUT: A COmpact spectrometer for measurements of neutrons at the ASDEX upgrade tokamak.

A COmpact Spectrometer for Measurements Of Neutrons at the ASDEX Upgrade Tokamak (COSMONAUT) has been developed for spectroscopy measurements of the 2.45MeV neutron emission from deuterium plasmas at the ASDEX Upgrade. The instrument is based on a CLYC-7 inorganic scintillator, whereby the detection of fusion neutrons occurs via their interaction with 35Cl nuclei in the detector crystal, leading to a peak in the detector response function and providing excellent neutron/gamma-ray discrimination capabilities. The diagnostics is installed along a radial line of sight and makes use of a digital system to record time resolved data for the whole duration of the discharge. Measurements in ASDEX Upgrade plasmas with neutral beam injection have been carried out and are successfully interpreted using state-of-the-art modeling codes. Next step applications of the diagnostics are in experiments aimed at generating energetic particles by ion cyclotron resonance heating schemes. In these scenarios, COSMONAUT will provide unique information on the acceleration of deuterons beyond the beam injection energy and on their confinement, for comparison with modeling.

Assessing the Impacts of Reservoirs on Riverine Dissolved Organic Matter: Insights From the Largest Reservoir in the Pearl River

AbstractExtensive reservoir construction has fragmented more than 70% of the world's rivers, significantly impacting river connectivity and carbon cycling. However, the response of riverine dissolved organic matter (DOM) to reservoir influence and its potential downstream effects remains unclear. In this study, we employed multiple analytical techniques, including Fourier transform ion cyclotron resonance mass spectrometry, radiocarbon dating, and environmental factor analysis, to investigate the dynamic changes in DOM and its controlling factors under different hydrological management regimes in the LongTan Reservoir, the largest reservoir in the Pearl River, which is the second largest river in China by water discharge. Our results indicate that the molecular diversity of riverine DOM is reduced in the reservoir. Oxygen‐rich and heteroatomic compounds, such as those containing nitrogen, sulfur, and phosphorus, are preferentially removed through enhanced photo‐ and biodegradation processes in the reservoir, particularly during the storage period. This leads to DOM that is enriched with oxygen‐poor compounds and shows a biodegraded Δ14C value downstream. This study highlights that the composition of riverine DOM is significantly altered by the reservoir, but these effects could potentially be mitigated by optimizing the outlet location.

An automated and high-throughput data processing workflow for PFAS identification in biota by direct infusion ultra-high resolution mass spectrometry.

The increasing recognition of the health impacts from human exposure to per- and polyfluorinated alkyl substances (PFAS) has surged the need for sophisticated analytical techniques and advanced data analyses, especially for assessing exposure by food of animal origin. Despite the existence of nearly 15,000 PFAS listed in the CompTox chemicals dashboard by the US Environmental Protection Agency, conventional monitoring and suspect screening methods often fall short, covering only a fraction of these substances. This study introduces an innovative automated data processing workflow, named PFlow, for identifying PFAS in environmental samples using direct infusion Fourier transform ion cyclotron resonance mass spectrometry (DI-FT-ICR MS). PFlow's validation on a bream liver sample, representative of low-concentration biota, involves data pre-processing, annotation of PFAS based on their precursor masses, and verification through isotopologues. Notably, PFlow annotated 17 PFAS absent in the comprehensive targeted approach and tentatively identified an additional 53 compounds, thereby demonstrating its efficiency in enhancing PFAS detection coverage. From an initial dataset of 30,332 distinct m/z values, PFlow thoroughly narrowed down the candidates to 84 potential PFAS compounds, utilizing precise mass measurements and chemical logic criteria, underscoring its potential in advancing our understanding of PFAS prevalence and of human exposure.

The mechanism on liquefaction of waste tire by ethanolysis

Thermo-chemical treatment methods are pivotal for effectively managing waste tire rubber (WTR) while promoting energy efficiency and emission reduction. This study investigates the liquefaction of WTR within a thermal ethanol environment, exploring temperature ranges (200-300 °C) and residence times (0-80 min) to discern the role of ethanol in tire degradation. Additionally, the study includes liquefaction experiments with cyclohexane as a solvent to contrast the effect of ethanol, and comparison between ethanol and water to elucidate solvent impact. Results indicate that at 300 °C for 40 minutes, oil yields peak at 53.05% for a WTR/ethanol mass ratio of 1:8 and at 51.64% for a ratio of 1:6, notably surpassing hydrolysis outcomes. By contracting with the results from the liquefaction with cyclohexane as solvent, the involvement of ethanol can facilitate liquid phase products, primarily alkenes and oxygen-containing compounds, attributed to aromatization inhibition. Heavy oils and heteroatomic compounds are characterized through Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS), detecting hydrocarbons and heteroatom compounds like NxOy, Ox, Sx, NxOySz, NxSy, and Nx. Notably, sulfur, a significant pollutant, predominantly manifests as sulfide in chars. These findings offer valuable insights into advancing waste tire recycling technologies.

Design of the electron cyclotron resonance (ECR) plasma source for the space plasma environment research facility.

The Space Plasma Environment Research Facility (SPERF) was built in Harbin to study the three-dimensional magnetic reconnection and wave-particle interactions relevant to space physics in laboratory settings. A 2.45GHz Electron Cyclotron Resonance (ECR) plasma source is adopted in the device to simulate the Earth's magnetosphere and achieve the scientific goals. In this paper, the design of the ECR plasma source is presented. The structures of the microwave source, the microwave transfer system, and the antenna are introduced. Additionally, the resonant surfaces are computed to predict the locations of microwave absorption. The absorption mechanisms of the microwave in the SPERF are also discussed. The discharge experiment demonstrates the utility of the ECR source in simulating the Earth's magnetosphere. The successful operation of the source indicates that the ECR discharge is a powerful tool for creating a plasma environment in a large plasma experimental device.

Rock-to-Pharma: Characterization of Shale Oil-Based Nonbiological Complex Drugs along the Production Process by High-Resolution Mass Spectrometry.

Antibiotic resistance has become a primary concern in medicine because of the overuse and misuse of classical pharmaceuticals. Recently, nonbiological complex drugs (NBCDs) have gained interest for their complex pharmacological profiles. Bituminosulfonates, which have lately been tentatively allocated toward NBCDs, are pharmacologically well-studied and show low potential in resistance development. However, molecular composition knowledge is limited. With this work, we present a comprehensive approach to investigate the manufacturing process of complex pharmaceuticals like bituminosulfonates on a molecular level via Fourier-transform ion cyclotron resonance mass spectrometry. The application of various hyphenations and ionization techniques comprehensively covers the entire mass and polarity range of the matrix, and the high sensitivity enables the identification of significant and minor chemical alterations caused by the multistep manufacturing process. The distillation of the shale crude oil eliminates highly aromatic PAH and PASH constituents. ESI(-) revealed strong PAH- and PASH-sulfonate formation after reacting the shale oil distillate with sulfuric acid. Increasing alkylation reduced the sulfonation yield, instead causing oligomerization side reactions, as observed by APPI analysis. Furthermore, multidimensional gas chromatography coupled with high-resolution mass spectrometry verified core structural motifs. With this work, we demonstrate the high potential of FT-ICR MS in NBCD process analysis. The results also give valuable information for future pharmacological investigations focusing on specific compound classes or properties.

Transformation of sedimentary dissolved organic matter in electrokinetic remediation catalogued by FT-ICR mass spectrometry

In electrokinetic remediation (EKR), the sedimentary dissolved organic matter (DOM) could impede remediation by scavenging reactive species and generating unintended byproducts. Yet its transformation and mechanisms remained largely unknown. This study conducted molecular-level characterization of the water-extractable DOM (WEOM) in EKR using negative-ion electrospray ionization coupled to 21 tesla Fourier transform ion cyclotron resonance mass spectrometry (21 T FT-ICR MS). The results suggested that ∼55 % of the ∼7,000 WEOM compounds identified were reactive, and EKR lowered their diversity, molecular weight distribution, and double-bond equivalent (DBE) through a combination of electrochemical and microbial redox reactions. Heteroatom-containing WEOM (CHON and CHOS) were abundant (∼ 35% of the total WEOM), with CHOS generally being more reactive than CHON. Low electric potential (1 V/cm) promoted the growth of dealkylation and desulfurization bacteria, and led to anodic CO2 mineralization, anodic cleavage of -SO and -SO3, and cathodic cleavage of -SH2; high electric potential (2 V/cm) only enriched desulfurization bacteria, and differently, led to anodic oxygenation and cathodic hydrogenation of unsaturated and phenolic compounds, in addition to cathodic cleavage of -SH2. The long-term impact of these changes on soil quality and nitrogen-sulfur-carbon flux may be need to studied to identify unknown risks and new applications of EKR.

Spectral Indices and Evidence of Wave–Wave Modulation in Observations of the Interplanetary Magnetic Field

We present wave and turbulence observations from the DSCOVR spacecraft during the 2017 September solar flare and coronal mass ejection (CME) events. On September 4–12, the spectral index within the magnetic field power spectral density inertial range was consistent with Kolmogorov −5/3. This is despite the 9 days being composed of a complex mix of different features, including solar flares, solar energetic particle events, and CMEs. When analyzing shorter time periods, the spectral index varies. For two days where there were consecutive CMEs, the index follows Kraichnan–Iroshinikov −3/2, while on two quiet days, it was a mixture of −1, −3/2, and −2. The inertial range spectral index taken over the entire 9 days hides or averages out spectral features that occur over shorter time periods. We use a more realistic estimate of the amount of Doppler shifting into the spacecraft frame a show that the break frequencies on most days were located close to the H+ cyclotron frequency. We present evidence of wave–wave modulation and suggest that lower-frequency waves in the solar wind can modulate the growth rates/propagation of ion cyclotron waves, providing a method to transfer energy in the solar wind to smaller scales. Furthermore, we suggest that the indices in the inertial range can be explained by combining containment due to wave generation/propagation and stochastic Brownian motion in the solar wind. When these two phenomena are equal, they combine to create a −3/2 index.

Applying Spatial Metabolomics To Investigate Age- and Drug-Induced Neurochemical Changes.

In an era when population aging is increasing the burden of neurodegenerative conditions, deciphering the mechanisms underlying brain senescence is more important than ever. Here, we present a spatial metabolomics analysis of age-induced neurochemical alterations in the mouse brain using negative ionization mode mass spectrometry imaging. The age-dependent effects of the acetylcholinesterase inhibitor tacrine were simultaneously examined. For ultrahigh mass resolution analysis, we utilized a Fourier-transform ion cyclotron resonance spectrometer. To complement this, a trapped ion mobility spectrometry time-of-flight analyzer provided high speed and lateral resolution. The chosen approach facilitated the detection and identification of a wide range of metabolites, from amino acids to sphingolipids. We reported significant, age-dependent alterations in brain lipids which were most evident for sulfatides and lysophosphatidic acids. Sulfatide species, which are mainly localized to white matter, either increased or decreased with age, depending on the carbon chain length and hydroxylation stage. Lysophosphatidic acids were found to decrease with age in the detailed cortical and hippocampal subregions. An age-dependent increase in the glutamine/glutamate ratio, an indicator of glia-neuron interconnection and neurotoxicity, was detected after tacrine administration. The presented metabolic mapping approach was able to provide visualizations of the lipid signaling and neurotransmission alterations induced by early aging and can thus be beneficial to further elucidating age-related neurochemical pathways.

Recent progress in improvement in ion cyclotron range of frequencies coupling and power absorption with new antennas of Experimental Advanced Superconducting Tokamak (EAST)

Efficient ion cyclotron range of frequencies (ICRF) wave heating requires good wave coupling at the plasma edge and good radio frequency power absorption in the plasma core. This study reviews recent progress in improving these two aspects of ICRF heating with the new two-strap antennas through various experiments and simulations on the Experimental Advanced Superconducting Tokamak (EAST). Our study shows that the ICRF coupling can be significantly improved by decreasing the parallel wave number, increasing the local scrape-off layer (SOL) density by midplane gas puffing, and increasing the global SOL density by decreasing the separatrix–antenna distance. It can also be improved by increasing the core plasma density, changing the divertor strike point position, and optimizing the antenna phasing. The core ICRF power absorption can be increased by optimizing the cyclotron resonance position and minority ion concentration and by applying new heating schemes such as three-ion heating. Although some of the methods have been previously studied on other devices, improving ICRF coupling by shifting the divertor strike point was tested on EAST for the first time. Quantitative characterization of these methods and the conclusions drawn from this study can provide important insights for achieving more efficient ICRF heating in current and future fusion machines.

Luminosity dependence of the multiple cyclotron lines in 4U 0115+63

The Be X-ray binary 4U 0115$+$63 underwent a giant outburst in 2023 with the X-ray luminosity of the source reaching 1038 erg s$^ $. During the outburst, two target of opportunity observations were made with NuSTAR The main goal of this work is to model the multiple cyclotron scattering features (CRSFs) present in 4U 0115$+$63 and study their dependence on the luminosity of the source. The 3$-$79 keV broadband X-ray coverage of NuSTAR allowed us to properly model the continuum and investigate the nature of the multiple cyclotron resonance scattering features present in the source spectrum. We used the epoch-folding technique to find the pulsation from the source and also studied the changes in the cyclotron line energy with an order of magnitude variation in the source luminosity. We detected five cyclotron lines during the 2023 outburst near 12, 16, 24, 34, and 47 keV. The sim 16 keV cyclotron line cannot be harmonically related to the other cyclotron lines at sim 12 keV and sim 24 keV. This indicates the presence of two fundamental lines in the spectrum of 4U 0115$+$63 at 12 keV and 16 keV. With the inclusion of the two latest NuSTAR observations, we have expanded the data set of the CRSF line center to encompass a broad range of luminosity. This enables us to comprehensively investigate the relationship between the centroid energy of the cyclotron lines and luminosity. The CRSF line center shows no anticorrelation with luminosity, unlike previously reported. Instead, a weak positive correlation is found in four out of the five detected cyclotron lines of 4U 0115$+$63. The luminosity variation of the two fundamental CRSFs could be well explained with the prediction from the collisionless shock model. A tentative negative correlation was observed in the fundamental CRSF at 16 keV and its harmonics beyond a "critical luminosity" of 1038 erg/s. This behavior was not present for the 12 keV fundamental CRSF and its harmonic at 24 keV.

Exploring transformation of dissolved organic matters and dissolved organic nitrogen in full-scale anammox wastewater treatment: Temperature and microbial roles

Variation of dissolved organic matters (DOM) in mainstream anammox process has received limited attention. This study systematically characterized DOM and dissolved organic nitrogen (DON) in a full-scale mainstream anammox wastewater treatment plant (WWTP) using spectroscopy and Fourier transform-ion cyclotron resonance mass spectrometry. Roles of bacterial community structures related with temperatures on DOM and DON transformations were analyzed. Results indicated that the WWTP removed highly bioavailable, S-containing DOM while producing more unsaturated, aromatic, and N-containing DOM. Higher relative abundances of Proteobacteria and Chloroflexi at low temperature resulted in greater removal rates of proteins, SMP-like and humic acid-like substances. At high temperature, higher relative abundance of Actinobacteriota increased lignin production. Principal component analysis revealed that temperature significantly impacted DOM characteristics compared to DON. These findings are crucial for understanding DOM and DON transformation during mainstream anammox WWTP

Interactions between Iron Minerals and Dissolved Organic Matter Derived from Microplastics Inhibited the Ferrihydrite Transformation as Revealed at the Molecular Scale.

Microplastic-derived dissolved organic matter (MP-DOM) is an emerging carbon source in the environment. Interactions between MP-DOM and iron minerals alter the transformation of ferrihydrite (Fh) as well as the distribution and fate of MP-DOM. However, these interactions and their effects on both two components are not fully elucidated. In this study, we selected three types of MP-DOM as model substances and utilized Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) and extended X-ray absorption fine structure (EXAFS) spectroscopy to characterize the structural features of DOMs and DOM-mineral complexes at the molecular and atomic levels. Our results suggest that carboxyl and hydroxyl groups in MP-DOM increased the Fe-O bond length by 0.02-0.03 Å through interacting with Fe atoms in the first shell, thereby inhibiting the transformation of Fh to hematite (Hm). The most significant inhibition of Fh transformation was found in PS-DOM, followed by PBAT-DOM and PE-DOM. MP-DOM components, such as phenolic compounds and condensed polycyclic aromatics (MW > 360 Da) with high oxygen content and high unsaturation, exhibited stronger mineral adsorption affinity. These findings provide a profound theoretical basis for accurately predicting the behavior and fate of iron minerals as well as MP-DOM in complex natural environments.

Electron Dynamics Associated With Advection and Diffusion in Self‐Consistent Wave‐Particle Interactions With Oblique Chorus Waves

AbstractChorus waves are intense electromagnetic emissions critical in modulating electron dynamics. In this study, we perform two‐dimensional particle‐in‐cell simulations to investigate self‐consistent wave‐particle interactions with oblique chorus waves. We first analyze the electron dynamics sampled from cyclotron and Landau resonances with waves, and then quantify the advection and diffusion coefficients through statistical studies. It is found that phase‐trapped cyclotron resonant electrons satisfy the second‐order resonance condition and gain energy from waves. While phase‐bunched cyclotron resonant electrons cannot remain in resonance for long periods. They transfer energy to waves and are scattered to smaller pitch angles. Landau resonant electrons are primarily energized by waves. For both types of resonances, advection coefficients are greater than diffusion coefficients when the wave amplitude is large. Our study highlights the important role of advection in electron dynamics modulation resulting from nonlinear wave‐particle interactions.

Independent-oscillator model and the quantum Langevin equation for an oscillator: a review

This review provides a brief and quick introduction to the quantum Langevin equation for an oscillator, while focusing on the steady-state thermodynamic aspects. A derivation of the quantum Langevin equation is carefully outlined based on the microscopic model of the heat bath as a collection of a large number of independent quantum oscillators, the so-called independent-oscillator model. This is followed by a discussion on the relevant ‘weak-coupling’ limit. In the steady state, we analyze the quantum counterpart of energy equipartition theorem which has generated a considerable amount of interest in recent literature. The free energy, entropy, specific heat, and third law of thermodynamics are discussed for one-dimensional quantum Brownian motion in a harmonic well. Following this, we explore some aspects of dissipative diamagnetism in the context of quantum Brownian oscillators, emphasizing upon the role of confining potentials and also upon the environment-induced classical-quantum crossover. We discuss situations where the system-bath coupling is via the momentum variables by focusing on a gauge-invariant model of momentum-momentum coupling in the presence of a vector potential; for this problem, we derive the quantum Langevin equation and discuss quantum thermodynamic functions. Finally, the topic of fluctuation theorems is discussed (albeit, briefly) in the context of classical and quantum cyclotron motion of a particle coupled to a heat bath.

An extensive analysis of SOL properties in high-δ plasmas in ASDEX Upgrade

A set of dedicated H-mode discharges with constant heating power combining Neutral Beam Injection and Electron Cyclotron Resonance Heating have been executed at the ASDEX Upgrade tokamak using a high triangularity magnetic geometry in order to investigate the impact of filamentary transport to divertor and non-divertor components. The evolution of upstream scrape-off layer (SOL) profiles have been correlated with dedicated separatrix quantities, mostly with the turbulence control parameter αt (Eich and Manz 2021 Nucl. Fusion 61 086016) describing the turbulence level at the separatrix. With increasing αt , a broadening of the upstream density profiles in the near-SOL together with the formation of a density shoulder in the far-SOL have been observed. This phenomenon is associated with an enhanced filamentary transport dominating the radial turbulent transport in the far-SOL and confirmed by means of the cooling water calorimetry on non-divertor components. The probe measurements conducted with the ball-pen probe-head mounted on the midplane manipulator and a retarding-field analyzer close to the limiter surface indicate that the key mechanism increasing the radial filamentary transport to the first wall is an increase of the particle flux Γr,fil , caused primarily by the packing fraction fPF,fil and the filament density ne,fil . At the same time, the electron temperature Te and ion temperature Ti measured close to the limiter surface show only small variations above αt > 0.5. Both the filamentary heat flux and the gross erosion derived from the first wall probe measurements reach a magnitude that should be considered in the design of future fusion reactors.

Study of the Electron cyclotron power deposition in TOMAS

Efficient Electron Cyclotron Resonance Heating (ECRH) breakdown and pre-ionization can be achieved with fundamental X-mode, while higher harmonics can introduce excessive stray radiation. Fundamental heating however is characterized by a low cut-off density, introducing additional power absorption mechanisms in the plasma. A good knowledge of these mechanisms is necessary to use fundamental X-mode as an efficient pre-ionization method. Numerous experiments were performed on the TOroidally MAgnetized System (TOMAS) to study the power deposition for ECRH in helium. It is a facility designed to study plasma production, wall conditioning and plasma-surface interactions and is operated by LPP-ERM/KMS at the FZ-Jülich. The influence of the injected power P EC and the magnetic field B 0 on the absorption mechanisms is examined, in order to reduce stray radiation and improve the absorption efficiency. This will allow to determine the best scenarios for plasma start-up and pre-ionization.