This paper investigates a novel method for exploring soliton behavior in ion transport across biological membranes. This study uses the Hirota bilinear transformation technique together with the Poisson–Nernst–Planck equation. A thorough grasp of ion transport dynamics is crucial in many different scientific fields since biological membranes are important in controlling the movement of ions within cells. By extending the standard equation, the suggested methodology offers a more thorough framework for examining ion transport processes. We examine a variety of ion-acoustic wave structures using the Hirota bilinear transformation technique. The different forms of solitons are obtained including breather waves, lump waves, mixed-type waves, periodic cross-kink waves, M-shaped rational waves, M-shaped rational wave solutions with one kink, and M-shaped rational waves with two kinks. It is evident from these numerous wave shapes that ion transport inside biological membranes is highly relevant, and they provide important insights that may have an impact on various scientific disciplines, medication development, and other areas. This extensive approach helps scholars dig deeper into the complexity of ion transport, illuminating the complicated mechanisms driving this essential biological function. Additionally, to show the physical interpretations of these solutions we construct the 3D and their corresponding contour plots by choosing the different values of constants. So, these solutions give us the better physical behaviors.