Investigation Of Traumatic Injuries Research Articles

MRNA MPS tissue identification assay to aid in the investigation of traumatic injuries

Molecular analysis of the RNA transcriptome from a putative tissue fragment should permit assignment to a specific organ since each tissue will exhibit a unique pattern of gene expression. Determination of the organ source of tissues from crime scenes may aid in shooting and stabbing investigations. We have developed a new prototype massively parallel sequencing (MPS) mRNA profiling assay for organ tissue identification, designed to definitively identify 13 organ/tissue types using a targeted panel of 48 mRNA biomarkers. The identifiable organs and tissues include brain, spinal cord, lung, trachea, liver, skeletal muscle, heart, kidney, adipose, intestine, stomach, skin and spleen. The biomarkers were chosen after iterative specificity testing of numerous candidate genes in various tissue types. The assay is very specific with little cross reactivity with non-targeted tissue, and can detect RNA mixtures from different tissues, including two- to five-tissue admixtures. The sensitivity of the assay was evaluated as well as assay reproducibility between library preparations and sequencing runs. We also demonstrate the ability of the assay to successfully identify the tissue source of origin in cadaver samples, tissue samples with varying post mortem intervals (PMI) and mock and bona fide casework samples. We are using the data to train a multivariate statistical model that predicts the tissue type based on the mRNA profile. By considering co-expression of markers the model can recognize distinct expression patterns in each tissue.

Prevalence of Physical Injuries on the Head, Neck and Entire Body in, Hilla, Iraq

The aim of our study was conducted to assess the clinical profile of all patients with bruising injury, skeletal injury, head injury, ocular injury and oral injury. This study also included investigation of traumatic injuries associated with fracture, covering the period from 2010 to 2015. Knowledge of these associated injuries provides useful strategies for patient care and prevention of further complications. Eighty three males and twenty seven female (age range 15–75 years) were included in the study. Injuries were distributed randomly over the body surface and were mostly located in the skeletal [n=60 (66%)], but those to the bruising [n=1 (0.91%)], head [n=41 (37.21%)], ocular [n=2 (1.18%)], and oral [n=1 (0.91%)].