Nanosized hydrogel particles prepared through inverse microemulsion polymerization of 2-acrylamido-2-methyl-1-propanesulfonic acid, using the combination of an oil soluble emulsifier (SPAN80) with a water soluble emulsifier (TWEEN 80), and precise determination of HLB range related to the formation of stable single phase microemulsions. The effect of crosslink density, water phase to oil phase ratio, and the hydrophilic-lipophilic balance (HLB) value on polymerization rate, particle size, and swelling ratio were investigated. It found that polymerization rate and particle size are strongly dependent on the water phase to oil phase ratio. Hydrogel samples prepared using oil soluble and water soluble initiators and the results showed that the initiator type had a great influence on monomer conversion and particle size. Effect of pH on equilibrium swelling of hydrogels was studied by dynamic light scattering and hydrogels showed pH-independent swelling behavior in a broad range of pH values. We also reported and discussed the crosslink density distribution in nanogels prepared by inverse microemulsion polymerization.