Interspecific Origin Research Articles

Study and evaluation of agrobiological indicators of grape varieties and hybrid forms in the conditions of Southern Dagestan

The article presents the results of many years of research on the study of the genetic fund of grapes concentrated in the ampelographic collection of the Dagestan selection experimental station for viticulture and vegetable growing. Selection and variety study of grapes play a major role in the accelerated promotion to the market and the introduction into industrial production of promising varieties for various uses, which is very important in our time. A comprehensive study of 104 varieties and hybrid forms of grapes of intraspecific and interspecific origin was carried out. The purpose of the study is an agrobiological assessment of promising varieties and hybrid forms of grapes of interspecific and intraspecific origin for various uses. Methods. The research was carried out at the ampelographic collection of the Dagestan selection experimental station for viticulture and vegetable growing, located in Derbent. The object of research was 104 varieties and hybrid forms of grapes of domestic and foreign selection. Of these, 30 table varieties, 70 technical varieties and 4 universal varieties. The zoned varieties Karaburnu (table) and Muscat white (technical) were used as a standard. The research was carried out according to the following methods: “Study of grape varieties” and “Agrotechnical research on the creation of intensive grape plantings on an industrial basis”. Results. The article presents the results of assessing the indicators of fruiting and fruitfulness of varieties and hybrid forms of grapes of interspecific and intraspecific origin. According to the indicators of fruiting (K1) and fruitfulness (K2) among table varieties and hybrid forms, the following are distinguished: SV-23-657, SV-12-304, Dekabrsky, Ialoveni sustainable, V-95-1, XI-36-6/100 of which K1 ranged from 0.94 to 1.33 and K2 from 1.17 to 1.51. Among the technical varieties and hybrid forms based on these indicators, the following were distinguished: Bianca, Viorica, Gift of Magaracha, Yubileiny Magaracha, Pervenets Magaracha, in which K1 ranged from 1.17-1.50 and K2 from 1.34-1.60. For most varieties and hybrid forms, the fruiting coefficient (K1) varies from 0.67 to 1.30, and the fruiting coefficient (K2) ranges from 1.03 to 1.42. In universal varieties, the fruiting coefficient (K1) ranges from 0.70-1.02 and the fruiting coefficient ranges from 0.96-1.31. According to the yield of table varieties, interspecific hybrids are distinguished: Dekabrsky, SV-12-304, SV-20-365, SV-23-657, XI-36-6/100 in which the yield per bush varies from 11.2 to 19.5 kg/bush. Among the technical varieties in terms of yield per bush, the following stand out: TSKHA-3-2, Pierrel, Bianka, SV-20-473, Strogoziya, Podarok Magaracha, SV-12-375, for which the yield per bush was 12.4-22.2 kg. Conclusion. The introduction of selected varieties and hybrids into production will ensure an increase in crop yields and will contribute to import substitution and ensure the country's food security. Individual varieties will be used as sources of valuable traits in breeding work.

First investigation into the genetic control of meiosis in sugarcane.

The sugarcane (Saccharum spp.) genome is one of the most complex of all. Modern varieties are highly polyploid and aneuploid as a result of hybridization between Saccharum officinarum and S. spontaneum. Little research has been done on meiotic control in polyploid species, with the exception of the wheat Ph1 locus harboring the ZIP4 gene (TaZIP4-B2) which promotes pairing between homologous chromosomes while suppressing crossover between homeologs. In sugarcane, despite its interspecific origin, bivalent association is favored, and multivalents, if any, are resolved at the end of prophase I. Thus, our aim herein was to investigate the purported genetic control of meiosis in the parental species and in sugarcane itself. We investigated the ZIP4 gene and immunolocalized meiotic proteins, namely synaptonemal complex proteins Zyp1 and Asy1. The sugarcane ZIP4 gene is located on chromosome 2 and expressed more abundantly in flowers, a similar profile to that found for TaZIP4-B2. ZIP4 expression is higher in S. spontaneum a neoautopolyploid, with lower expression in S. officinarum, a stable octoploid species. The sugarcane Zip4 protein contains a TPR domain, essential for scaffolding. Its 3D structure was also predicted, and it was found to be very similar to that of TaZIP4-B2, reflecting their functional relatedness. Immunolocalization of the Asy1 and Zyp1 proteins revealed that S. officinarum completes synapsis. However, in S. spontaneum and SP80-3280 (a modern variety), no nuclei with complete synapsis were observed. Importantly, our results have implications for sugarcane cytogenetics, genetic mapping, and genomics.

ТЕХНОЛОГИЧЕСКАЯ ОЦЕНКА СОРТА ВИНОГРАДА МЕГРАБУЙР В УСЛОВИЯХ НИЖНЕГО ПРИДОНЬЯ

The paper presents the results of a technological assessment of red technical grapevine variety of interspecific origin of Armenian breeding Megrabuir, growing in the Don Ampelographic Collection named after Y.I. Potapenko" (Novocherkassk, Rostov region) in 2020–2022. The research was carried out in the laboratory of ampelography and technological assessment of grapevine varieties, in microwinemaking conditions. The weight of one batch of grapes was 5−15 kg. Samples of dry rose and red, as well as rose and red liqueur wines were prepared. The main physical and chemical parameters of must and wine have been determined. They all met the criteria of The National Standards. An organoleptic assessment of wine materials showed that all samples (dry red and rose, and liqueur) had a color characteristic of a certain type of wine, had a bright, complex aroma, with tones of berries, and light shades of flowers and herbs, had a balanced taste, and harmonious composition. Based on the results of the research, conclusions were drawn that the technical grapevine variety Megrabuir is promising for the preparation of various types of wines (dry rose, red, liqueur).

IMPROVEMENT OF GENETIC POTENTIAL CULTURAL FORM OF EGGPLANT DUE TO HYBRIDIZATION WITH A LINE OF INTERSPECIES ORIGIN

As a result of domestication, the created varieties and hybrids F1 of the cultivated form of eggplant (Solanum melongena L.) are characterized by low genetic variability and unsatisfactory adaptive potential for a set of quantitative traits useful for breeding and therefore require a significant renewal of the genetic plasma due to interspecific hybridization with other representatives of the Solanum genus. The goal is to create, for adaptive breeding, the starting material of a cultured form of eggplant (Solanum melongena L.) due to hybridization with a line of interspecies origin, derived from the fertile progeny of the hybrid F1(S. melongena / S. aethiopicum Gilo group). Methods: general scientific, measuring, weighting, variational statistics (calculation of root mean square deviation (σ), coefficient of variation (V)). Results. As a result of the hybridization of varieties and lines of the cultivated form of eggplant (Solanum melongena L.) with the line of interspecific origin BC2[F5 (Pavlotas-20 / Almaz variety)], the hybrid progeny F2 and the inbred progeny I1, derived from the hybrids F1, which according to the levels of manifestation quantitative traits valuable for breeding had a steady tendency to exceed the standard Almaz variety. The hybrid F2(BiboF8 / ВС2[F5(Pavlotas-20 / Almaz variety)]) (Xmed = 1101.8 g/plant) was highly productive. The high level of manifestation of the traits “number of fruits per plant” (Xmed = 5.56 pcs.) and “average weight of the fruit” (Xmed = 263.1 g) was noted by the hybrid F2(ВС2[F5(Pavlotas-20 / Almaz variety)] / BiboF8). Compared to the standard variety, the same F2 hybrid showed trait of early ripeness (by 2 days) in terms of the duration of the period from the appearance of mass seedlings to the technical ripeness of the fruits. In the studied sample of different eggplant progeny, those in which the coefficient of variation (V) did not exceed the limit of 33.33% already in the second year of breeding, which, according to the theory of statistical analysis, is a criterion of genetic stabilization of the corresponding quantitative trait at the level of the varietal population of plants. According to this criterion, samples F1 (Aisberh variety / ВС2[F5(Pavlotas-20 / Almaz variety)])I1 and F1(ВС2[F5(Pavlotas-20 / Almaz variety)] / BiboF8)I1, according to the trait “average fruit weight” – F2(Long Violet / ВС2[F5(Pavlotas-20 / Almaz variety)]), according to the trait “productivity of one plant” – F1(ВС2[F5(Pavlotas-20 / Almaz variety)] / BiboF8)I1. Discussion. Valuable genetic sources obtained on an interspecies basis will be involved in the breeding process of creating highly adaptive varieties and F1 hybrids of eggplant, suitable for organic growing technologies due to high abiotic resistance and the presence of genes for resistance to fungal diseases (Fusarium oxysporum f.sp. melongenae, Ralstonia solanacearum).

Supernumerary B Chromosomes of Tetragonisca fiebrigi Share Repeat Content with Standard Chromosome Set of both T. fiebrigi and Tetragonisca angustula (Apidae: Meliponini)

The stingless bees Tetragonisca angustula and Tetragonisca fiebrigi are widely distributed in Brazil, and both are commonly known as “jataí.” Our goal was to investigate the possible origin of the B chromosomes in T. fiebrigi, a cytotaxonomic trait that differentiates T. fiebrigi from T. angustula. We analyzed diploid chromosome number (2n), B chromosome incidence, patterns of constitutive heterochromatin, and in situ localization of different repetitive DNA probes in T. angustula and T. fiebrigi. Both species displayed 2n = 34, with similar karyotype structures. One to three B chromosomes were observed in T. fiebrigi only. Constitutive heterochromatin was distributed on one arm of all chromosomes in both species, and T. fiebrigi B chromosomes were mainly heterochromatic with one euchromatic extremity. The (GA)₁₅ and (CAA)₁₀ microsatellite probes marked the euchromatic arms of all chromosomes in both species without marking the B chromosomes. The 18S ribosomal DNA (rDNA) probe marked 10 chromosomes in T. angustula and 6 A chromosomes in T. fiebrigi with an additional marking on 1B in individuals with 3B. The Tan-Bsp68I repetitive DNA probe marked the heterochromatic portion of all T. fiebrigi A and B chromosomes. This probe also marked the heterochromatic portion of all T. angustula chromosomes; therefore, both alternative hypotheses to the B chromosome origin are possible: (i) from the A chromosome complement of T. fiebrigi (intraspecific origin); or (ii) a by-product of genome reshuffling following the hybridization between T. fiebrigi and T. angustula (interspecific origin).

The Nectariy grape cultivar for the northern zone of Russian industrial viticulture

The domestic assortment of fruit and berry crops should include new varieties with high biological plasticity, group resistance against adverse environmental conditions, different ripening duration, and sufficiently high quality of the final products. These requirements are highly relevant in grape breeding. Nektariy, a new grape сultivar of interspecific origin, was admitted to state testing in 2021. This cultivar was created at Y.I. Potapenko All-Russian Research Institute of Viticulture and Winemaking under the state task for cultivation in the northern zone of industrial viticulture of Russia and for production of high-quality Muscat wine of various types. The studies were carried out according to conventional ampelography methods. The Nektariy agrobiological characteristics include the following: a cultivar of industrial purpose with an early ripening period. During the 2018–2022 period, the production period lasted, on average, 115 days under the effective heat sum of 2501°C. Nektariy is characterized by high winter hardiness and the budburst percentage from 84 to 100. Under the negative temperature sum of 586°C and the absolute minimum temperature of minus 24.6 °C, 49 % of live buds was registered. The number of fruiting shoots comprised 90–98 % under the fruiting coefficient of 1.4. On average, the yield per plant was 5.9 kg on a single-plane trellis under the planting scheme of 3×1.5 m. The cultivar ampelographic description is as follows: androgyne flower, cylindro-conical bunches of medium density weighing on average 153 g. The knot is very short, green, sometimes woody, green ridge. The berry is rounded, greenish-yellow, with an intense pruinous stain, with an average weight of 1.9 g. The flesh is juicy with a muscat aroma. In order to create a passport of the cultivar, the Nektariy leaf blade was measured in terms of 33 parameters using the SIAMS Mesoplant soft ware for analyzing the morphology and structure of plants. This cultivar is characterized by a high accumulation of sugars up to 29.3 g/100 cm3 under a moderate acidity of 5.8 g/dm3 . The resistance to major fungal diseases is high, which allows this cultivar to be used in breeding as a donor of resistance genes to mildew. The shoot growth strength is medium; shoots mature well. The affinity with rootstock varieties is high. The tasting score of dry wine and liqueur wine averages 8.65 and 8.8 points, respectively, on a ten-point evaluation scale. The cultivation of Nektariy grape is economically efficient due to low expenditures associated with uncovered cultivation and disease protection measures. Nektariy is used for the production of high-quality Muscat wine with the profitability 140 %. Nektariy is recommended for uncovered cultivation in wine-making Russian regions with an absolute minimum of winter temperatures up to minus 26 °C.

Meiotic Behaviors of Allotetraploid Citrus Drive the Interspecific Recombination Landscape, the Genetic Structures, and Traits Inheritance in Tetrazyg Progenies Aiming to Select New Rootstocks.

Sexual breeding at the tetraploid level is a promising strategy for rootstock breeding in citrus. Due to the interspecific origin of most of the conventional diploid citrus rootstocks that produced the tetraploid germplasm, the optimization of this strategy requires better knowledge of the meiotic behavior of the tetraploid parents. This work used Genotyping By Sequencing (GBS) data from 103 tetraploid hybrids to study the meiotic behavior and generate a high-density recombination landscape for their tetraploid intergenic Swingle citrumelo and interspecific Volkamer lemon progenitors. A genetic association study was performed with root architecture traits. For citrumelo, high preferential chromosome pairing was revealed and led to an intermediate inheritance with a disomic tendency. Meiosis in Volkamer lemon was more complex than that of citrumelo, with mixed segregation patterns from disomy to tetrasomy. The preferential pairing resulted in low interspecific recombination levels and high interspecific heterozygosity transmission by the diploid gametes. This meiotic behavior affected the efficiency of Quantitative Trait Loci (QTL) detection. Nevertheless, it enabled a high transmission of disease and pest resistance candidate genes from P. trifoliata that are heterozygous in the citrumelo progenitor. The tetrazyg strategy, using doubled diploids of interspecific origin as parents, appears to be efficient in transferring the dominant traits selected at the parental level to the tetraploid progenies.

Изучение трансформации компонентного состава плодов яблони в сидр

Cider is obtained by fermenting mashed apples of special cider varieties. The Russian State Register of Selection Achievements includes 476 varieties of apples, some of which can be used in commercial cider production. To identify potential cider cultivars, food scientists study the transformation of chemicals in apple mash during fermentation.
 The research involved 16 samples of apple mash and cider obtained from apples of foreign and domestic selection. Their physicochemical, biochemical, and sensory parameters were identified using standard methods, as well as the methods of high-performance capillary electrophoresis and gas chromatography.
 The samples revealed a wide range of concentrations of titratable acids, phenolic substances, ascorbic acids, and phenolcarboxylic acids, depending on the cultivar. After fermentation, the content of ascorbic acid decreased by an average of 76%. The content of phenolcarboxylic acids in the cider samples increased by an average of 51% compared with the apple mash samples. The ciders contained succinic, oxalic, lactic, and acetic acids, which were not registered in the apple mash, and the concentration of amino acids doubled. The cider from the Virginia variety had the best sensory profile, and it also had the highest concentration of phenolic substances (1121.6 mg/dm3).
 In this research, the best characteristics belonged to the ciders from apple varieties with a complex interspecific origin, obtained by a complex of polyploidy and distant hybridization methods, and with high concentrations of sugars and phenolic substances in the apple mash. Further research will test varieties of other origins and physicochemical properties for their potential use in cider, vodka, and calvados production.

ИЗУЧЕНИЕ СОРТОВ ВИНОГРАДА МЕЖВИДОВОГО ПРОИСХОЖДЕНИЯ В УСЛОВИЯХ РОСТОВСКОЙ ОБЛАСТИ

The studies were carried out in order to clarify the agrobiological and uvological characteristics of grape varieties of interspecific origin, selection of Federal Rostov Agrarian Research Center, in non-covering culture. The work was carried out in the northern zone of industrial viticulture in 2018–2021 on the ampelographic collection (Rostov region) of grapes. We studied 3 technical varieties (Grushevsky white, Saperavi northern, Flower) and 3 table varieties (Vostorg, Talisman, Rusmol). The collection was founded in 2008 by grafted vine seedlings (rootstock Kober 5BB), planting scheme 3×1.5 m. The height of the stem of the bushes was 70-100 cm. There were no severe winters during the study period. On average, over the years of the study, the number of blossoming eyes and fruitful shoots in industrial varieties was 82...97 and 83...89%, respectively, in canteens - 71...95 and 57...79%, respectively. According to the duration of the growing season, the varieties were distributed as follows: Vostorg - very early (109 days), Talisman - early (125 days), Rusmol, Tsvetochny, Saperavi northern - medium (139 ... 143 days), Grushevsky white - medium-late (146 days). The yield of technical varieties varied from 4.5 (Saperavi Severniy) to 8.4 kg/bush (Tsvetochny); canteens - from 5.5 (Talisman) to 9.7 kg/bush (Rapture). According to the average weight of the bunch, Grushevsky white (312 g) stood out among the technical varieties, surpassing other varieties of this group by 111 ... 200 g, among table varieties - Rusmol (619 g), ahead of other table varieties by 133 ... varieties was high and very high (from 20.7 to 23.4 g/100 cm3), in table varieties - medium and low (from 16.2 to 18.9 g/100 cm3). Tasting scores of wines from berries of technical grades were at the level of 8.4…8.5 points, from table fruits – 7.8…8.5 points. In general, changes in the climatic conditions of the Lower Don region did not affect the economically valuable traits of the studied varieties of domestic selection.

Формирование бессемянных ягод у винограда сорта Кодрянка при применении регуляторов роста

The article presents the results of studying the influence of growth regulators on the formation of seedless berries in the grape variety of interspecific origin Codrianka. The size of berries of different types has been studied depending on the presence or absence of seeds in them. The preparations contributing to the formation of large seedless berries in the Codrianka variety have been identified. Keywords: GRAPES, GROWTH REGULATORS, SEEDLESSNESS, UVOLOGICAL ANALYSIS, SMALL DIAMETER BERRIES, PARTHENOCARPY

ОСОБЕННОСТИ СОСТАВА ОРГАНИЧЕСКИХ КИСЛОТ СУСЕЛ И ВИН ИЗ КРАСНЫХ СОРТОВ ВИНОГРАДА МЕЖВИДОВОГО ПРОИСХОЖДЕНИЯ

The aim of the work was to study the composi-tion of organic acids of musts and wines made from red grapevine varieties of interspecific origin growing in the conditions of Rostov re-gion. The objects of the study were must and wines prepared under conditions of micro winemaking from red grapevine varieties of interspecific origin bred by ARRIV&W, a branch of FSBSI FRASC ‒ Denisovsky, Cab-ernet Severniy, Augusta, Cabernet Sauvignon (control), according to the classical technology adopted for red dry wines. As a result of the study the following results were obtained. In must of all studied varieties the predominance of tartaric acid over malic acid was observed in several times, while in the interspecific grape-vine varieties the concentration of tartaric acid was 5‒6 times higher than malic acid, while in the control only 2.5 times higher. The greatest amount of tartaric acid was observed in the must from Cabernet Severniy grapes ‒ 5.6 g/dm3. Malic acid accumulation was signifi-cantly lower in all variants (0.48‒1.1 g/dm3) compared with the control Cabernet Sauvignon (2 g/dm3). There was a slight accumulation of succinic acid in the must from Denisovsky and Cabernet Severniy grapes. In all variants, with the exception of Augusta wine, the concentra-tion of tartaric and malic acids decreased. The total proportion of tartaric and malic acids in the experimental wines was 38‒74.7 % of all acids in wine. The highest concentration of succinic acid was observed in wines from Den-isovsky and Cabernet Sauvignon varieties.

БИОХИМИЧЕСКИЕ И ОРГАНОЛЕПТИЧЕСКИЕ СВОЙСТВА ВИН ИЗ КРАСНЫХ СОРТОВ ВИНОГРАДА СЕЛЕКЦИИ ВНИИВиВ

The aim of the work is to study the biochemi-cal and organoleptic properties of red table wines obtained from grape varieties of inter-specific origin growing in the vineyards of the Novocherkassk branch of the experimental field of ARRIV&W. The research was carried out on red grape varieties of interspecific origin Denisovskiy, Cabernet severniy, Avgusta. After the research, the following results were obtained. The largest stock of phenolic and nitrogenous substances was ob-served in the must from the Denisovsky and Augusta grapevine varieties. The highest con-tent of extractive, phenolic and coloring sub-stances was noted in the experimental wine from the Augusta variety. The content of phenolic and coloring substances in the stud-ied sample from the Cabernet Severny variety was almost two times less than in the control Cabernet Sauvignon. According to organolep-tic analysis, the highest tasting score was ob-tained from the Augusta grape variety (8.7 points)

Effective Technological Schemes for the Cultivation of Industrial Open-Earth Vineyards in the Don Area

Over the long years of the development of agro-technological approaches to the methods of grape growing, researchers paid special attention to the issues of pruning grape bushes, the methods of their formation and maintenance and planting plans. In the environmental conditions of the Lower Don of the Russian Federation, many years of research were conducted in order to establish the optimal parameters of various agro-technological methods used in the vineyard (the methods of maintaining, forming, pruning and the rates of bush loading) through the example of a promising variety of Crystal grapes of interspecific origin. At the same time, a prerequisite for this research was the link with the scheme of planting vines and its effect on the productivity of uncultivated vineyards, as well as the quality of the crop. In this study, we proposed the technological and economic advantages of industrial and intensive methods of maintaining and forming vineyards on an industrial basis. These methods were new for the Don conditions. We conducted research and studied various ways of maintaining, forming, pruning and loading grape bushes in the Rostov Region, in the conditions of the experimental field, which increased the use of photosynthetic active radiation (PAR) in the production process on standard open-earth vineyards of industrial and intensive type, when cultivating Crystal grape varieties of interspecific origin. Processing and analysis of the results of the application of various agricultural methods in the experimental areas will reveal the most optimal ones from an economic point of view.
 Keywords: grapes, architectonics, management method, productivity, biomass

The utility of ISSRs for the identification of interspecific hybrids between pearl millet (Pennisetum glaucum [L.] R.Br.) × napier grass (Pennisetum purpureum Schumach)

AbstractInterspecific hybrids between pearl millet (Pennisetum glaucum) and napier grass (Pennisetum purpureum) give rise to perennial fodder crops characterized by high biomass, broad clumps and good palatability. These hybrids are triploid and developed by hand pollination of napier grass pollen on pearl millet panicles. The progeny shows a high percentage of pearl millet genotype due to self-pollination in the female parent. Identification of hybrids at a young stage based on morphological characters is difficult. DNA-based molecular markers have high discriminating power and were used to assess genetic differences between hybrids and their parents. Genetic diversity was studied in 18 pearl millet × napier grass hybrids along with their parents and two released national checks using inter simple sequence repeat (ISSR) markers. Eight ISSR primers gave rise to 125 bands, of which 120 bands were polymorphic. Polymorphic information content and ISSR primer index ranged from 0.40 to 0.49 and 8.88 to 11.14, respectively. The hybrids showed the presence of unique bands, besides those shared with male and female parents. Female (pearl millet) parents formed a separate group in the dendrogram constructed based on ISSR polymorphism. The male (napier grass) parents formed a separate group along with hybrids, indicating a higher similarity of hybrids with the male parents. Principal component analysis and STRUCTURE analyses showed a similar grouping. The close resemblance of hybrids to the male parents confirmed their interspecific origin. The study revealed that ISSR marker analysis could be a quick and reliable method to identify interspecific hybrids at an early stage of growth.

Characterization of Odor-Active Compounds of Ichang Lemon (Citrus wilsonii Tan.) and Identification of Its Genetic Interspecific Origin by DNA Genotyping.

Ichang lemon is a citrus fruit whose rind gives off a delicious and much appreciated fragrance and flavor. The volatile components of the fruit peel of Ichang lemon were investigated by GC-MS and GC-O (AEDA method). Simultaneously, its genetic origin was identified by using diagnostic SNP markers specific to ancestral species and multiallelic SSR and InDel markers. Ichang lemon combines three ancestral genomes (Citrus maxima, Citrus ichangensis, and Citrus reticulata) and may be a pummelo × Yuzu hybrid. Although the major compounds of the Ichang lemon aromatic profile were present in Citrus junos, a few pummelo-specific compounds were also detected, such as indole and nootkatone, in agreement with its maternal lineage. 3-Methyl-3-sulfanylbutyl acetate, reported to occur in passion fruit and brewed coffee, was identified by GC-MS, GC-QTOF-MS, and GC-FTIR for the first time in citrus. This odor-active compound has a sulfurous, tropical fruity, green note.

Analysis of the development of grafted grape seedlings on a nursery garden of different graft-rootstock combinations

In 2019-2020, experiments were laid on the fields of the ARRIV&W – Branch of FRARC with the aim of experimentally determining the phenotypic signs of compatibility of scion-rootstock combinations with the participation of grape varieties of interspecific origin Denisovskiy and Prestige and rootstock varieties Kober 5 BB and Riparia Rupestris 101-14 introduced to the State Register of Breeding Achievements of the Russian Federation, approved for use. According to the results of the studies, it can be concluded that the selection of the optimal scion-rootstock for growing the necessary scion varieties has a direct effect on the yield of seedlings, which, with the same level of agricultural technology, will minimize production costs for inoculating seedlings and repairing grape plantations. The influence of the rootstock variety on the phenotypic characteristics of the scion variety has been established. The yield of seedlings is the most objective indicator of the compatibility of the scion-rootstock combination. According to the research results 2019-2020. it was found that the yield of first-class seedlings of the Denisovsky and Prestige varieties significantly depends on the used rootstock variety. The Riparia Rupestris 101-14 rootstock has the highest yield of seedlings of the Denisovskiy variety, 45.4%, which is 21.6% more than on the Kober 5 BB rootstock variety. On the Denisovskiy variety, the seedling yield was 48.0%, which is 39.9% more than on the Kober 5 BB rootstock variety.

Biochemical characteristics of roots and quality of own-rooted planting material of grape varieties tolerant to phylloxera when using biologically active substances and hormones

The aim of the research is to conduct a comparative assessment of the biochemical status of roots and the influence of physiologically active compounds on the formation of the root system of phylloxera-tolerant grape varieties. It was established, that the hormonal status and the content of BAS in the root system of phylloxera-tolerant varieties of interspecific origin are different. It is assumed that the different survival rate of cuttings of V. vinifera L. varieties and varieties of interspecific origin are due to different hormonal status, in particular, the level of endogenous auxins and the degree of gene expression, including exogenous use of auxin drugs. On varieties of interspecific origin, with the non-root use of a solution of FAS based on cytokinin, auxin and trophic factor, in a ratio of 10:1:25, the formation of a powerful root system, an increase in the number of heel roots and the number of roots with a large diameter, an increase in the trophic potential of the elements of the root system compared with the pre-planting use of auxin preparation (NUC) is observed. this will contribute to the better development and early fruiting of root-related plants in the following years.

МОРФО-БИОЛОГИЧЕСКИЕ ОСОБЕННОСТИ ФОРМИРОВАНИЯ ДИАФРАГМЫ У IN VITRO И EX VIRO РАСТЕНИЙ ВИНОГРАДА МЕЖВИДОВОГО ПРОИСХОЖДЕНИЯ

Clonal micropropagation of grape varieties of interspecific origin Moscow white (Vitis amurensis Rupr. × Vitis vinifera L.) showed that on the 40th day of the animation stage, a monopodial type of branching is typical for micro-plants, and there is no diaphragm in all nodes of micro-shoots. On the 120th day of growing in containers, the researchers revealed that the nodes of the plant shoots, from the 1st to the 6th, have a monopodial branching type. Parenchymal cells which form an incomplete diaphragm are present in the seventh node. A fully formed diaphragm appears from the eighth node, and the branching type switches to a sympodial-monopodial one.

Insights into the heterochromatin evolution in the genus Melipona (Apidae: Meliponini)

The species of the four Melipona subgenera can be subdivided into two cytogenetic groups: species belonging to Group I are characterized by having low heterochromatin content in their karyotypes ( 50%). To study the patterns of heterochromatin accumulation in this genus, we isolated moderate to highly repetitive sequences in M. (Michmelia) mondury (Mmon probe) and M. (Melikerria) fasciculata (Mfas probe) obtained based on the renaturation kinetics of C0t − 1 DNA and used these fragments as probes to hybridize in thirteen species belonging to the four Melipona subgenera. The Mmon probe marked all chromosomes in all Michmelia species and additionally the secondary constriction of M. (Eomelipona) puncticollis. The Mfas probe showed positive markings only within Melikerria. These results indicate the independence of the heterochromatin growth between Michmelia and Melikerria. Absence of markings in the regular set of chromosomes in M. (Melikerria) quinquefasciata and positive markings in the Bs, with the Mfas probe, suggests an interspecific origin of these chromosomes. We also hypothesize about the possible scenario that led to the heterochromatin growth in the genus Melipona.

Origin, clonal diversity, and evolution of the parthenogenetic lizard Darevskia unisexualis

BackgroundThe hybridization of female D. raddei and male D. valentini gave rise to the parthenogenetic Caucasian rock lizard Darevskia unisexualis. A previously identified genetic polymorphism in the species consisted of one common and two allozyme clones. Analysis of microsatellites and single nucleotide polymorphisms (SNPs) from the three species yields estimates of clonal diversity and tests the hypothesis of a single origin for D. unisexualis.ResultsGenotyping and sequencing of four microsatellite-containing loci for 109 specimens of D. unisexualis, 17 D. valentini, and 45 D. raddei nairensis identified 12 presumptive clones, including one widespread and 11 rare clones. Most individuals in some localities had a rare clone. Clone-specific alleles in D. unisexualis were compared with those of the parental species. The results inferred a single hybridization event. Post-formation mutations best explain the less common clones.ConclusionsInterspecific analyses identify alleles inherited by D. unisexualis from its bisexual ancestors. SNP analyses fail to reject the hypothesis of a single interspecific origin of D. unisexualis, followed by microsatellite mutations in this initial clone. Microsatellites detect higher clonal diversity in D. unisexualis compared to allozymes and identify the likely origins of clones. Our approach may be applicable to other unisexual species whose origins involve interspecific hybridization.