Interspecific Contacts Research Articles

Interacting effects of surface water and temperature on wild and domestic large herbivore aggregations and contact rates

Abstract Earth's climate is rapidly changing, bringing forth questions of how domestic and wild animals will alter their behaviour in response to increasing temperatures and dryland expansion. Dwindling water availability will likely impact animal behaviour and water foraging, potentially increasing animal aggregations and interspecific contacts. These interspecific contacts are especially important for competition, predation and disease transmission among wildlife and domestic animals. In this study, we analysed interspecific wildlife and cattle contacts using two years of camera trap data at an experimental water manipulation site at a conservancy in central Kenya. We found that on average, the hourly probability of any interspecific contact was approximately 3.4 times higher at water sources versus drained water sources and 18 times higher than surrounding matrix areas, and that this relationship was amplified by dry and hot conditions. Species‐specific analyses revealed variation in the magnitude of responses across wildlife and domestic cattle, although all animals had approximately 2–3 times higher interspecific contact probability with other species at water in hot conditions versus other conditions. Notably, we observed the largest behavioural changes for relatively water‐independent species, such as giraffe, which had 3.6 times higher interspecific contact probability at water sources in hot versus other conditions. Synthesis and applications. These findings show how elevated temperatures that will become increasingly common with future climate changes can increase interspecific contacts around critical water resources. In mixed wildlife‐livestock systems, maintaining wildlife‐only water sources may be a practical management tool to mitigate human‐wildlife conflict and disease transmission at this interface, especially during dry and hot conditions.

The Phenomenon of a Mixed Colony: the Case of Lasius brunneus and Lasius umbratus (Hymenoptera, Formicidae)

The aim of this work is to study to find out why, in the case of temporary social parasitism, a mixed colony of Lasius brunneus (Latreille, 1798) and Lasius umbratus (Nylander, 1846) exists in this state for many years. In 2023, a mixed colony of L. brunneus (host species) / L. umbratus (temporary social parasite) was discovered in Moldova. This mixed colony is located at the same site where another mixed colony containing Lasius fuliginosus (Latreille, 1798), L. umbratus disappeared in 2017. Video recordings of the intensity of movement of workers of the two species along the trails were made to study the frequency of antennal contacts and to calculate the colony population. Based on the previously used algorithms, it was assumed that the queen oviposition rate of L. umbratus is 1.5 times higher than that of L. niger, while that of L. brunneus is comparable to that of Lasius niger (Linnaeus, 1758). Chi-square test and post-hoc Dunn's test were used for statistical analysis of the data. Mathematical modelling was used to analyse the rate of colony development. Results of the analysis of worker activity on the trail showed that L. brunneus had 11 times more foragers than L. umbratus. A high number of interspecific antennal contacts between workers was recorded. The population of the mixed colony was estimated to be 48.880 L. brunneus and 24.433 L. umbratus workers. Mathematical modelling confirmed the failure of L. brunneus monogyny in a mixed colony and suggested that high L. brunneus abundance is ensured by multiple queens (oligogyny). Probably in 2021, the L. brunneus colony moved to a new habitat, which was invaded by the L. umbratus queen. After the invasion, several L. brunneus queens survived, allowing a mixed colony to exist.

Home range overlap between small Indian mongooses and free roaming domestic dogs in Puerto Rico: implications for rabies management

The small Indian mongoose (Urva auropunctata) is the primary terrestrial wildlife rabies reservoir on at least four Caribbean islands, including Puerto Rico. In Puerto Rico, mongooses represent a risk to public health, based on direct human exposure and indirectly through the transmission of rabies virus to domestic animals. To date, the fundamental ecological relationships of space use among mongooses and between mongooses and domestic animals remain poorly understood. This study is the first to report mongoose home range estimates based on GPS telemetry, as well as concurrent space use among mongooses and free roaming domestic dogs (FRDD; Canis lupus familiaris). Mean (± SE) home range estimates from 19 mongooses in this study (145 ± 21 ha and 60 ± 14 ha for males and females, respectively) were greater than those reported in prior radiotelemetry studies in Puerto Rico. At the scale of their home range, mongooses preferentially used dry forest and shrubland areas, but tended to avoid brackish water vegetation, salt marshes, barren lands and developed areas. Home ranges from five FRDDs were highly variable in size (range 13–285 ha) and may be influenced by availability of reliable anthropogenic resources. Mongooses displayed high home range overlap (general overlap index, GOI = 82%). Home range overlap among mongooses and FRDDs was intermediate (GOI = 50%) and greater than home range overlap by FRDDs (GOI = 10%). Our results provide evidence that space use by both species presents opportunities for interspecific interaction and contact and suggests that human provisioning of dogs may play a role in limiting interactions between stray dogs and mongooses.

Drought legacy effects on fine-root-associated fungal communities are modulated by root interactions between tree species

With climate change, the frequency of severe droughts is predicted to increase globally, resulting in increased forest dieback. Although fine-root systems and their associated fungi are considered crucial for tree nutrient exchange after a drought period and consequently for tree recovery, post-drought dynamics remain poorly understood. We rewatered mature European beech and Norway spruce after a 5-year experimental summer drought to shed light on belowground recovery processes. Therefore, we tracked the fine-root parameters growth, vitality, and mycorrhization in monospecific rooting zones with intraspecific root contact and mixed rooting zones with interspecific root contact of both tree species during the first 3 months of recovery, and we analyzed compositions of their root-associated fungal communities by DNA- and RNA-ITS2 sequencing. During recovery, the fine-root parameters differed between both tree species, with only minor effects of the tree rooting zone. Root-associated fungal communities showed no significant response to irrigation within 3 months after drought release. The rooting zone was the dominating factor affecting the root-associated fungal diversity, the abundance of trophic modes, and the response of individual saprotrophic and ectomycorrhizal (ECM) species. Furthermore, an analysis of the most abundant fungal species revealed that for ECM fungi, drought tolerance was common and for saprotrophs, a facultative, root-associated lifestyle. These results suggest that tree species-specific fungal communities are stable despite previous long-term drought and are closely associated with tree species-specific response patterns related to root survival and recovery. Moreover, an association between saprotrophic fungi and roots might be a strategy to support fungal drought survival.

Behavioural interactions between sika deer and Japanese serows: are larger and gregarious ungulates dominant?

Abstract In ungulate assemblages, although interspecific differences in body size and gregariousness are thought to have a direct impact on winning or losing in interference competition, this has not been fully investigated. We observed a total of 64 instances of interspecific contact between larger-gregarious sika deer and smaller-solitary Japanese serows over eight years field observations. Deer-to-serow aggression were never observed. Whereas, serows showed antagonistic behaviour, such as walk push and chasing, to deer, but could rarely displace deer. Serows showed higher alertness to deer than deer did to serows. These results suggest that larger-gregarious ungulates do not necessarily exclude smaller-solitary ones and serows are sensitive to the presence of deer. Differences in aggressiveness and alertness between deer and serows may reflect differences in territoriality: serows may be more sensitive to the invasion of deer into their territory. Serows’ sensitivity to deer may have a negative effect on their population.

Prevalence of pathogens in sympatric Ixodes ricinus and Dermacentor reticulatus ticks in Eastern Poland and their potential impact on oral-anal contacts between ticks.

Little is known about interspecific contacts between ticks. Therefore, this study focused on the investigation of factors that may influence interspecific contacts between Ixodes ricinus and Dermacentor reticulatus ticks. Ixodes ricinus males and D. reticulatus females involved in oral-anal contacts (group I) and questing specimens with no such behaviour (group II) collected in eastern Poland were examined using molecular techniques to detect Borrelia burgdorferi s.l. (Bb), Rickettsia spp. (Rs), Anaplasma phagocytophilum, Babesia microti, and Toxoplasma gondii. An extremely high infection rate of Bb and Rs was determined in I. ricinus males (in groups I: 100% and 46.15% and group II: 90% and 40%, respectively) and D. reticulatus females (in group I: 84.61% and 61.53% and in group II: 90% and 20%, respectively). The prevalence of other pathogens in these ticks was substantially lower. Co-infection with pathogens was detected in approximately 53% of ticks. The study suggests that tick-borne pathogens may have influenced the sexual behaviour of their vectors. The oral-anal contacts between I. ricinus and D. reticulatus ticks are probably stimulated by Bb and/or Rs. The presence of five pathogens and numerous co-infections in the analysed ticks indicates a risk of various human infectious diseases in the study region. Further studies are required to clarify the implications of oral-anal interspecific tick interactions.

The farther, the closer: geographic proximity and niche overlap versus genetic divergence in Caucasian rock lizards

Abstract Caucasian rock lizards (Darevskia) are a speciose group with 42 described species from the Caucasus Mountain region. We selected 11 species from Georgia and adjacent territories to explore whether their distribution depends on the distribution of congeneric taxa. We estimated two measures for each species pair: (1) niche overlap between all pairs of the studied species; (2) the extent of contact between the ranges of individual species. We then calculated the correlation between these measurements and the time of divergence between the species. The results showed that in sympatric and parapatric (but not allopatric) species, there is a highly significant correlation between the divergence time and the extent of the contact, as well as between the divergence time and the overlap of suitable habitats. The ranges of the evolutionarily closest species have the smallest extent of contact. Species separated later have overlapping ranges, although are separated by altitude. The most distant species, irrespective of their phenotype, are commonly found in the same location. We suggest that a gradual increase in the contact extent is compensated for by increasing the effectiveness of other mechanisms of niche shift or behavioural isolation and hence the frequency of potentially deleterious interspecific contacts is kept low.

BIOGEOGRAPHICAL CHARACTERISTICS OF SMALL MAMMALS OF THE HERB-BUNCHGRASS STEPPE AND ADJACENT TERRITORIES IN THE SOUTH TRANS-URAL REGION (KURGAN OBLAST)

A brief history of the study of small mammals of the South Trans-Ural region is discussed. On the basis of data obtained using two generally accepted zoological methods (ditch with pitfalls and trap-lines), materials on the small mammal communities are summarized. The species composition, groups of background and dominant species, total abundance of insectivores and rodents were shown to be largely determined by the specific method of animal surveys. In the herb-bunchgrass steppe 18 species of animals were found in the ditch with pitfalls and 11 species in the snap traps surveys. The core of small mammals in this subzone was the common vole, herb wood mouse, tundra and common shrews. In the herb-bunchgrass steppe, as well as in the forest-steppe subzone and subtaiga in spring-summer period, insectivorous and mouse-like rodents were most densely concentrated in near-water biotopes, where there is their high overlap and intra- and interspecific contact, which is especially important for the spread and persistence of various infections. In the subtaiga of the South Trans-Ural region, the optimum complex of conditions is formed for forest-meadow forms, i.e. the Laxmann's and taiga shrews, Siberian chipmunk and bank vole. At the same time, open-space species such as the tundra shrew, southern birch mouse and steppe lemming inhabit the herb-bunchgrass steppe most densely. Of the 22 small mammal species recorded in the South Trans-Ural region in 2020-2022, only one of them, the common shrew, was among the dominant species in all subzones. The taiga shrew and the Siberian chipmunk, which are peculiar only to the subtaiga and do not penetrate into the more southern subzones of the studied territory, made up a special originality among the recorded species of insectivores and rodents of the Southern Trans-Urals.

Diverse partitiviruses hosted by the ectomycorrhizal agaric Hebeloma mesophaeum and the natural transmission of a partitivirus between phylogenetically distant, sympatric fungi

Mycorrhizal fungi host diverse mycoviruses that contribute to our understanding of their diversity and evolution. Here we report on the identification and complete genome characterization of three novel partitiviruses naturally infecting the ectomycorrhizal fungus Hebeloma mesophaeum. During NGS derived viral sequence analyses, we identified a partitivirus that is conspecific with the previously reported partitivirus (LcPV1) described from a saprotrophic fungus Leucocybe candicans. The two distinct fungal specimens inhabited the same vicinity of a campus garden. RdRp sequences encoded by the LcPV1 isolates from both host fungi was found to be identical. Bio-tracking studies revealed that viral loads of LcPV1 drop significantly in L. candicans but not in H. mesophaeum within four years period. The physical proximity of the mycelial networks of both fungal specimens implied the occurrence of a virus transmission event with unknown mechanism. Nature of this virus transmission was discussed in relation to transient interspecific mycelial contact hypothesis.

World Health Organization critical priority Escherichia coli clone ST648 in magnificent frigatebird (Fregata magnificens) of an uninhabited insular environment.

Antimicrobial resistance is an ancient natural phenomenon increasingly pressured by anthropogenic activities. Escherichia coli has been used as markers of environmental contamination and human-related activity. Seabirds may be bioindicators of clinically relevant bacterial pathogens and their antimicrobial resistance genes, including extended-spectrum-beta-lactamase (ESBL) and/or plasmid-encoded AmpC (pAmpC), in anthropized and remote areas. We evaluated cloacal swabs of 20 wild magnificent frigatebirds (Fregata magnificens) of the Alcatrazes Archipelago, the biggest breeding colony of magnificent frigatebirds in the southern Atlantic and a natural protected area with no history of human occupation, located in the anthropized southeastern Brazilian coast. We characterized a highly virulent multidrug-resistant ST648 (O153:H9) pandemic clone, harboring blaCTX–M–2, blaCMY–2, qnrB, tetB, sul1, sul2, aadA1, aac(3)-VIa and mdfA, and virulence genes characteristic of avian pathogenic (APEC) (hlyF, iroN, iss, iutA, and ompT) and other extraintestinal E. coli (ExPEC) (chuA, kpsMII, and papC). To our knowledge, this is the first report of ST648 E. coli co-producing ESBL and pAmpC in wild birds inhabiting insular environments. We suggest this potentially zoonotic and pathogenic lineage was likely acquired through indirect anthropogenic contamination of the marine environment, ingestion of contaminated seafood, or by intra and/or interspecific contact. Our findings reinforce the role of wild birds as anthropization sentinels in insular environments and the importance of wildlife surveillance studies on pathogens of critical priority classified by the World Health Organization.

Some features of small mammalian’s ectoparasite communities formation in the mixed forests of the Nizhny Novgorod Region

The paper deals with some features of small mammalians ectoparasite communities formation in the mixed forests of the Nizhny Novgorod Region. It has been shown that on rodents and insectivores in the mixed forests and in the ecotone community from forest to agrocoenosis typical ectoparasite communities are observed. Only rare species demonstrate specificity in the choice of hosts. Two species of rodents Myodes glareolus and Apodemus flavicollis, being typically forest species, are characterized by the highest biodiversity of ectoparasites and high rates of their abundance. The presence of the same species of ectoparasites on the different species of small mammals indicates possible contacts between rodents and insectivores in the ecosystem and exchange of parasites. Using the method of principal components (PCA), the factors influencing parasite community have been identified. It has been shown that out of 10 factors influencing the small mammalian ectoparasites community, 5 are the most significant. Many factors influence the communities of ectoparasites of small mammals. The degree of that effect depends on different mammalian and mites biology. Factors regulate the number of parasite populations, and as a result, stable communities functioning for a long time are formed. Parasites, not showing specificity in the choice of hosts during interspecific contacts of animals in biocoenoses, spread from forest habitats to neighboring territories.

Host preferences inhibit transmission from potential superspreader host species.

Host species that are particularly abundant, infectious and/or infected tend to contribute disproportionately to symbiont (parasite or mutualist) maintenance in multi-host systems. Therefore, in a facultative multi-host system where two host species had high densities, high symbiont infestation intensities and high infestation prevalence, we expected interspecific transmission rates to be high. Instead, we found that interspecific symbiont transmission rates to caged sentinel hosts were an order of magnitude lower than intraspecific transmission rates in the wild. Using laboratory experiments to decompose transmission rates, we found that opportunities for interspecific transmission were frequent, where interspecific and intraspecific contact rate functions were statistically indistinguishable. However, most interspecific contacts did not lead to transmission events owing to a previously unrecognized transmission barrier: strong host preferences. During laboratory choice experiments, the symbiont preferred staying on or dispersing to its current host species, even though the oligochaete symbiont is a globally distributed host generalist that can survive and reproduce on many snail host species. These surprising results suggest that when managing symbiont transmission, identifying key host species is still important, but it may be equally important to identify and manage transmission barriers that keep potential superspreader host species in check.

Feeding sites promoting wildlife-related tourism might highly expose the endangered Yunnan snub-nosed monkey (Rhinopithecus bieti) to parasite transmission

An increasing number of studies have found that the implementation of feeding sites for wildlife-related tourism can affect animal health, behaviour and reproduction. Feeding sites can favour high densities, home range overlap, greater sedentary behaviour and increased interspecific contacts, all of which might promote parasite transmission. In the Yunnan snub-nosed monkey (Rhinopithecus bieti), human interventions via provisioning monkeys at specific feeding sites have led to the sub-structuring of a group into genetically differentiated sub-groups. The fed subgroup is located near human hamlets and interacts with domesticated animals. Using high-throughput sequencing, we investigated Entamoeba species diversity in a local host assemblage strongly influenced by provisioning for wildlife-related tourism. We identified 13 Entamoeba species or lineages in faeces of Yunnan snub-nosed monkeys, humans and domesticated animals (including pigs, cattle, and domestic chicken). In Yunnan snub-nosed monkeys, Entamoeba prevalence and OTU richness were higher in the fed than in the wild subgroup. Entamoeba polecki was found in monkeys, pigs and humans, suggesting that this parasite might circulates between the wild and domestic components of this local social–ecological system. The highest proportion of faeces positive for Entamoeba in monkeys geographically coincided with the presence of livestock and humans. These elements suggest that feeding sites might indirectly play a role on parasite transmission in the Yunnan snub-nosed monkey. The implementation of such sites should carefully consider the risk of creating hotspots of disease transmission, which should be prevented by maintaining a buffer zone between monkeys and livestock/humans. Regular screenings for pathogens in fed subgroup are necessary to monitor transmission risk in order to balance the economic development of human communities dependent on wildlife-related tourism, and the conservation of the endangered Yunnan snub-nosed monkey.

From Protein to Pandemic: The Transdisciplinary Approach Needed to Prevent Spillover and the Next Pandemic.

Pandemics are a consequence of a series of processes that span scales from viral biology at 10−9 m to global transmission at 106 m. The pathogen passes from one host species to another through a sequence of events that starts with an infected reservoir host and entails interspecific contact, innate immune responses, receptor protein structure within the potential host, and the global spread of the novel pathogen through the naive host population. Each event presents a potential barrier to the onward passage of the virus and should be characterized with an integrated transdisciplinary approach. Epidemic control is based on the prevention of exposure, infection, and disease. However, the ultimate pandemic prevention is prevention of the spillover event itself. Here, we focus on the potential for preventing the spillover of henipaviruses, a group of viruses derived from bats that frequently cross species barriers, incur high human mortality, and are transmitted among humans via stuttering chains. We outline the transdisciplinary approach needed to prevent the spillover process and, therefore, future pandemics.

Anoplura (Lice) of small mammals in the South Trans-Urals

For this the research, 2035 individuals of small mammals of 18 species were examined in order to find out the presence of obligate hematophages (Anoplura); from those 984 lice of 5 species were collected. The species composition of Anoplura, the indices of occurrence, infestation and the abundance of Anoplura on the Insectivora and Rodentia were analyzed. There have been noted repeated cases of Anoplura parasitizing the mammals unusual for them, which reflects interspecific contacts of small mammals. The features of the biotopic confinement of the parasite were considered.

Domestic Dogs and Wild Foxes Interactions in a Wildlife-Domestic Interface of North-Central Chile: Implications for Multi-Host Pathogen Transmission.

Domestic dogs (Canis familiaris) often cohabite at interfaces shared by humans and wildlife, interacting with wild canids as predators, prey, competitors and reservoirs of several multi-host pathogens, such as canid-borne micro and macro parasites that could impact on wildlife, livestock and public health. However, spatio-temporal patterns of indirect interactions as promoters of pathogen transfer between domestic and wild canids are largely unknown. In this study, we used camera traps to describe the activity patterns and habitat use of dogs, chilla (Lycalopex griseus) and culpeo (Lycalopex culpaeus) foxes and identify the local-scale factors that may affect the frequency of dog-fox interactions through an anthropization gradient of the Coquimbo region, Chile. We assessed local-scale variables that may predict the number of interactions between dogs and foxes, and compared the time interval between dog-culpeo and dog-chilla interactions. Our findings suggested that closeness to urbanized zones predicts the frequency of indirect interactions between dogs and foxes. We found higher number of dog-fox interactions (60 interactions) at a periurban site adjacent to two coastal towns (Tongoy and Guanaqueros), compared to other two more undisturbed sites (12 interactions) increasingly distanced from urbanized areas. We showed that dogs interacted more frequently with chilla foxes (57 interactions) than with culpeo foxes (15 interactions), and the first interaction type occurred almost exclusively at the periurban site, where dogs and chillas were more frequently detected than in the other sites. We detected a marked temporal segregation between dogs and foxes, but dog-chilla interactions resulted in shorter time intervals (2.5 median days) compared to dog-culpeo interactions (7.6 median days), suggesting a higher potential risk of pathogen spillover between the first species pairing. Based on previous studies, we suggest periurban zones may constitute a potential focus of pathogen exposure between dog and fox populations in the study area. Our research contributes to improving the knowledge on the spatio-temporal patterns of interspecific contact between invasive and native carnivores within the context of multi-host pathogen dynamics. Our outcomes will inform theoretical epidemiological models designed to predict and minimize the contact risk between domestic and threatened species, guiding effective control strategies at the wildlife-domestic interface.

Modeling current and potential distributions of mammal species using presence-only data: A case study on British deer.

AimDecisions on wildlife conservation, management, and epidemiological risk are best based on robust evidence. The continual improvement of species distributions, such that they can be relied upon in decision‐making, is important. Here we seek to refine aspects of a generic modelling approach and improve the utility of species distribution maps.LocationGreat Britain (GB).MethodsWe applied a modeling framework based on hierarchical Bayesian species distribution models exploiting opportunistic occurrence records from citizen science datasets to predict both current and potential distributions for each of the six deer species known to be present in GB. Using the resulting maps, we performed a simple analysis of the overlap between species to illustrate possible contact, which we interpret as the relative risk of potential disease spread given an introduction.ResultsPredicted distribution maps showed good agreement with the broader scale occurrence reported by a recent national deer survey with an average True Skill Statistics and AUC of 0.69 and 0.89, respectively. Aggregation of the maps for all species highlighted regions of central and eastern England as well as parts of Scotland where extensive areas of range overlap could result in interspecific contact with consequences for risk assessments for diseases of deer. However, if populations are allowed to expand to their predicted potential, then areas of overlap, and therefore disease interspecific transmission risk, will become extensive and widespread across all of mainland Britain.Main conclusionsThe generic modeling approach outlined performed well across all of the deer species tested, offering a robust and reliable tool through which current and potential animal distributions can be estimated and presented. Our application, intended to inform quantitative risk assessments, demonstrates the practical use of such outputs to generate the valuable evidence required to inform policy decisions on issues such as management strategy.

Poultry population dynamics and mortality risks in smallholder farms of the Mekong river delta region

BackgroundPoultry farming is widely practiced by rural households in Vietnam and the vast majority of domestic birds are kept on small household farms. However, smallholder poultry production is constrained by several issues such as infectious diseases, including avian influenza viruses whose circulation remains a threat to public health. This observational study describes the demographic structure and dynamics of small-scale poultry farms of the Mekong river delta region.MethodFifty three farms were monitored over a 20-month period, with farm sizes, species, age, arrival/departure of poultry, and farm management practices recorded monthly.ResultsMedian flock population sizes were 16 for chickens (IQR: 10–40), 32 for ducks (IQR: 18–101) and 11 for Muscovy ducks (IQR: 7–18); farm size distributions for the three species were heavily right-skewed. Muscovy ducks were kept for long periods and outdoors, while chickens and ducks were farmed indoors or in pens. Ducks had a markedly higher removal rate (broilers: 0.14/week; layer/breeders: 0.05/week) than chickens and Muscovy ducks (broilers: 0.07/week; layer/breeders: 0.01–0.02/week) and a higher degree of specialization resulting in a substantially shorter life span. The rate of mortality due to disease did not differ much among species, with birds being less likely to die from disease at older ages, but frequency of disease symptoms differed by species. Time series of disease-associated mortality were correlated with population size for Muscovy ducks (Kendall’s coefficient τ = 0.49, p-value < 0.01) and with frequency of outdoor grazing for ducks (τ = 0.33, p-value = 0.05).ConclusionThe study highlights some challenges to disease control in small-scale multispecies poultry farms. The rate of interspecific contact and overlap between flocks of different ages is high, making small-scale farms a suitable environment for pathogens circulation. Muscovy ducks are farmed outdoors with little investment in biosecurity and few inter-farm movements. Ducks and chickens are more at-risk of introduction of pathogens through movements of birds from one farm to another. Ducks are farmed in large flocks with high turnover and, as a result, are more vulnerable to disease spread and require a higher vaccination coverage to maintain herd immunity.

Physiological pathways to rapid prosocial evolution.

Dogs (Canis lupus familiaris) descend from wolves (Canis lupus) sharing the same ecological niche of cooperative hunters, as humans. Initially, humans and wolves were competitors starting interspecific communication in order to avoid risk of injury. The evolutionary continuity of mammalian brains enabled interspecific prosocial contacts between both of them, which reduced stress, and enabled behavioral cultures leading to genetic isolation of those wolves. Dogs are the first domesticated animal living together with humans for about 25,000 years. Domestication means decreased aggression and flight distance toward humans, thus changes in the stress axis are crucial. The hypothesis of Active Social Domestication considers genetic selection as a necessary prediction but not a sufficient explanation of dog domestication. In addition, dog domestication is suggested to be an epigenetic disclosure. Due to changed stress activity, epigenetic mechanisms affect cerebral receptor activity and regulate transposon expressions, thus shaping brain function and behavior. Interspecific prosocial contacts initiated via serotonin release an enzymatic cascade enhancing, epigeneti-cally, the glucocorticoid negative feedback loop. Reduced chronic stress improved social learning capability and inhibitory control. Over time, those wolves could integrate themselves into human social structures, thus becoming dogs. In analogy, human mental skills, such as creating art and culture, might have also improved during the Upper Paleolithic.

A unique phenomenon of oral-anal contact between ticks observed in two tick species Ixodes ricinus and Dermacentor reticulatus.

For the first time in the world literature, the presented study describes oral-anal contact between two sympatric Ixodes ricinus and Dermacentor reticulatus ticks, which are the vectors of many pathogens in Europe. Among the specimens collected in nature, an I.ricinus male was found with the hypostome and chelicerae inserted in the anal aperture of a D.reticulatus female. Given the biological and physiological traits of both species and microorganisms colonising these arthropods, it seems that the interspecific oral-anal contacts between ticks may be an unknown alternative route of transmission of pathogenic and symbiotic microorganisms present in ticks' alimentary tract, faeces, body surface, and in the haemolymph and organs, taking place when the male's mouthparts are inserted into the female's body. The authors suggest that interspecific contacts between ticks in natural conditions can contribute to the spread of some icroorganisms among different tick species and their hosts..