International Space Station Experiment Research Articles

Space experiments using superconducting magnet technology

We report on a fast-track experiment on the International Space Station (ISS) with the Earth’s magnetic field, which has been recognized as a major task for a better understanding of our planet. For this purpose, YBCO bulk superconductors have been prepared, transported and stored on board the ISS. The properties of single grain superconducting bulk will be investigated before and after the ISS experiments. In parallel, the magnetic interaction between BEarth and our YBCO bulk was investigated. An external 3D Helmholtz coil cancels unwanted electromagnetic fields down to 5 μT. Highly sensitive flux-gate sensors monitored the magnetic configuration as the ceramic plate was cooled to cryogenic temperatures. Shielding and field concentration effects of the Earth’s magnetic field were observed and discussed. Thermally induced magnetic flux compression for future long-duration space missions was successfully tested, enabling future spacecraft shielding and braking functions.

An investigation of phase change induced Marangoni-dominated flow patterns using the Constrained Vapor Bubble data from ISS experiments

Kinetic models of liquid-vapor phase change often implicitly assume that the interface is in equilibrium. This equilibrium assumption can be justified for large flat interfaces far from the source of thermal energy, but it breaks down when the liquid surface is near a solid wall, or there is significant interface curvature. The Constrained Vapor Bubble (CVB) experiments conducted on the International Space Station (ISS) provide a unique opportunity to probe this common assumption and also provide unique data and insight into phase change-driven flow physics. The CVB experiment consists of a quartz cuvette partially filled with pentane such that a vapor bubble is formed at the center. The setup is heated and cooled at opposite ends, resulting in simultaneous evaporation and condensation. CVB data from the NASA Physical Science Informatics (PSI) database was used to reconstruct the entire 3D interface shape using interferometric image analysis and obtain an estimate of the net heat input to the bubble. The reconstructed interface shape is used to develop a liquid-only CFD model embedded with a custom-built “active surface” method that sets a variable interfacial temperature/phase change flux boundary condition. Phase change flux varies in both the axial and transverse directions, leading to a small (∼1 K) but discernible temperature variation along the liquid-vapor interface. The positive phase change flux near the heater end (denoting evaporation) gradually reduces and becomes negative near the cooler end (denoting condensation), resulting in an axial bulk flow of liquid from the cold to the hot end. There is also a higher flux in the thin film as opposed to the thick film, resulting in a transverse bulk flow. However, the interfacial temperature gradients along both axial and transverse directions induce a separate thermocapillary flow in a direction opposite to the bulk flows, leading to complex “wavy” flows with recirculation. A qualitative analysis of the flow pattern is presented in this paper and correlated with optical signatures from experimental images.

Heat transfer and interfacial flow physics of microgravity flow boiling in single-side-heated rectangular channel with subcooled inlet conditions – Experiments onboard the International Space Station

This study is the culmination of a long-term collaborative effort between researchers from the Purdue University Boiling and Two-Phase Flow Laboratory (PU-BTPFL) and the NASA Glenn Research Center to investigate gravitational effects on flow boiling and flow condensation. The science and design concepts for this large-scale effort were initiated in 2011 and included several studies detailing various aspects of two-phase fluid physics in both Earth gravity and microgravity, culminating in construction of the large-scale experimental facility named “Flow Boiling and Condensation Experiment (FBCE)”. The experiment was launched to the International Space Station (ISS) in August 2021. Following the successful installation of FBCE, equipped with the Flow Boiling Module (FBM), onboard the ISS and completion of several safety checks, flow boiling experiments were performed for five months from February 2022 until July 2022. This resulted in a large flow boiling database covering broad ranges of operating parameters and heating configurations spanning several research objectives. This study investigates microgravity flow boiling of n-perfluorohexane with subcooled inlet in a single-side-heated rectangular channel of dimensions 114.6-mm heated length, 2.5-mm heated width, and 5.0-mm height. Key operating parameters investigated are mass velocity (199.90 – 3200.13 kg/m2s), inlet subcooling (0.10 – 45.76°C), and inlet pressure (113.30 – 164.29 kPa). Images and image sequences acquired via high-speed-video are presented to elucidate the interfacial flow physics. To analyze and explain the effects of various parameters in microgravity, heat transfer results are presented as flow boiling curves, streamwise profiles of wall temperature and heat transfer coefficient, and parametric trends of local and averaged heat transfer coefficient. Mass velocity and inlet subcooling significantly influenced most of the aforementioned aspects of flow boiling, whereas effects of inlet pressure were comparatively insignificant. Although the data and observed flow physics might be different, the parametric effects and trends in microgravity are similar to vertical upflow in Earth gravity. Some cases, especially low mass velocities, high heat fluxes, and large degrees of inlet subcooling, experienced temporally anomalous flow behaviors caused by two-phase flow instabilities manifesting as flow reversals and resulted in deviations in overall trends. Severe thermodynamic non-equilibrium is observed throughout the channel. Overall, FBCE's ISS experiments were successful for subcooled inlet with single-sided heating of rectangular channel, and the collected data well established the various effects on flow boiling physics in highly controlled long-term microgravity conditions.

Ground Testing of the 16th Materials International Space Station Experiment Materials

External spacecraft materials play an important role in satellite protection from the harsh space environment. Research has shown that the physical, chemical, and optical properties of matter change continuously as a result of exposure to solar radiation and aggressive chemical species produced in Earth’s upper atmosphere. Thorough knowledge of the material properties’ evolution throughout a planned mission lifetime helps to improve the reliability of spacecraft. Moreover, the establishment of correlation factors between true space exposure and accelerated space weather experiments at ground facilities enables accurate prediction of on-orbit material performance based on laboratory-based testing. The presented work aims to evaluate the radiation effects of a low-Earth-orbit environment, namely, exposure to the high-energy electrons and atomic oxygen (AO) of heritage and novel spacecraft material selection. The studied materials represent the “flight duplicates” of samples that are launched as a part of the 16th Materials International Space Station Experiment Flight Facility (MISSE-FF) mission in 2022.

Measurement of High-energy Cosmic-Ray Proton Spectrum from the ISS-CREAM Experiment

The Cosmic Ray Energetics And Mass for the International Space Station (ISS-CREAM) experiment successfully recorded data for 539 days from 2017 August to 2019 February. We report the energy spectrum of cosmic-ray protons from the ISS-CREAM experiment at energies from 1.60 × 103 to 6.55 × 105 GeV. The measured spectrum deviates from a single power law. A smoothly broken power-law fit to the data, including statistical and systematic uncertainties, shows the spectral index change at 9.0 × 103 GeV from 2.57 ± 0.03 to 2.82 ± 0.02 with a significance of greater than 3σ. This bump-like structure is consistent with a spectral softening recently reported by the balloon-borne CREAM, DAMPE, and NUCLEON, but ISS-CREAM extends measurements to higher energies.

Comprehensive analyses of plant hormones in etiolated pea and maize seedlings grown under microgravity conditions in space: Relevance to the International Space Station experiment “Auxin Transport”

Functional relationships between endogenous levels of plant hormones in the growth and development of shoots in etiolated Alaska pea and etiolated Golden Cross Bantam maize seedlings under different gravities were investigated in the “Auxin Transport” experiment aboard the International Space Station (ISS). Comprehensive analyses of 31 species of plant hormones of pea and maize seedlings grown under microgravity (μg) in space and 1 g conditions were conducted. Principal component analysis (PCA) and a multiple regression analysis with the dataset from the plant hormone analysis of the etiolated pea seedlings grown under μg and 1 g conditions in the presence and absence of 2,3,5-triiodobenzoic acid (TIBA) revealed endogenous levels of auxin correlated positively with bending and length of epicotyls. Endogenous cytokinins correlated negatively with them. These results suggest an interaction of auxin and cytokinins in automorphogenesis and growth inhibition of etiolated Alaska pea epicotyls grown under μg conditions in space. Less polar auxin transport with reduced endogenous levels of auxin increased endogenous levels of cytokinins, resulting in changing the growth direction of epicotyls and inhibiting growth. On the other hand, almost no close relationship between endogenous plant hormone levels and growth and development in etiolated maize seedlings grown was observed under μg conditions in space, as per Schulze et al. (1992). However, endogenous levels of IAA in the seedlings grown under μg conditions in space were significantly higher than those grown on Earth, similar to the cases of polar auxin transport already reported.

Flow visualization, heat transfer, and critical heat flux of flow boiling in Earth gravity with saturated liquid‐vapor mixture inlet conditions – In preparation for experiments onboard the International Space Station

This study investigates flow boiling of n-Perfluorohexane with saturated two-phase mixture inlet in a rectangular channel of dimensions 114.6 mm heated length, 2.5 mm width, and 5 mm-height. The experiments were performed as part of the Mission Sequence Testing of the Flow Boiling and Condensation Experiment's (FBCE) Flow Boiling Module (FBM) in the vertical upflow configuration in Earth gravity using the same experimental system that was launched to the International Space Station (ISS) in August 2021. The operating parameters varied are heating configuration (single- and double-sided), mass velocity (380–2400 kg/m2s), inlet quality (0.011–0.519), and inlet pressure (120–179 kPa). High-speed video photographs are presented to explain the two-phase flow patterns within the channel's heated length. Flow patterns are constituted by low-density and high-density fronts moving along the channel, with the high-density fronts gradually reducing in length due to evaporation. Heat transfer results in terms of flow boiling curves, streamwise wall temperature profiles, streamwise heat transfer coefficient profiles, and average heat transfer coefficients are presented and trends discussed. CHF data from the present experiments are combined with prior databases to compile a consolidated FBCE-CHF database for saturated inlet to expand the ranges of operating conditions and include other flow orientations in Earth gravity. Experimental CHF trends are also discussed. The interfacial lift-off model shows a good CHF predictive accuracy evidenced by a mean absolute error of 11.97% for this consolidated database after constraining it to mass velocities greater than or equal to 500 kg/m2s. Finally, this study confirmed reliability of the upcoming ISS experiments for saturated inlet conditions and the collected Earth-gravity data will be compared to ISS microgravity data.

A Model for the Anomalous Velocity-Undercooling Behaviour of Levitated Al-Ni Alloys On-board the International Space Station

Al-Ni alloys (for Ni < 45 at.%) show a unique property in that, over at least part of the accessible undercooling range, the recalescence velocity measured in electromagnetically levitated samples is observed to decrease as the undercooling increases. This result has been subject to careful validation, including microgravity experiments utilising the TEMPUS levitation facility on-board the International Space Station (ISS). In these experiments, anomalous growth is observed to coincide with a recalescence morphology comprising multiple circular growth fronts [Herlach et al. Phys. Rev. Mat. 3, 073,402 (2019)], termed “scales”. In this paper we present an analysis of high speed video data from the ISS experiments in which we show that such scale-like growth is consistent with a recalescence front that is initially confined to a thin layer on the surface of the sample. This then nucleates a slower, radial inward growth, which is consistent with microstructures observed in Al-Ni droplets. We show that such surface recalescence would be favoured for samples which were surface enriched in Ni, wherein the recalescence velocity (at fixed nucleation temperature) increases rapidly with Ni-concentration. Moreover, it is shown that the anomalous velocity behaviour can be matched in all compositions studied if the surface enhancement in Ni is a linear function of the nucleation temperature with a gradient of 0.03 at.% K−1. Analysis of historical results from the literature indicates that such surface Ni-enhancement may have been present, but overlooked, in other experiments on Al-rich Al-Ni droplets.

Asymmetric Sawtooth Microstructure-Induced Vapor Mobility for Suppressed Buoyancy Conditions: Terrestrial Experiment and Design for ISS Experiments

The lack of buoyancy forces in microgravity stagnates vapor bubbles on heated electronic surfaces, raising local surface temperatures above device operating limits. Though flow boiling can help dissipate higher heat fluxes, the addition of pumps and regulated flow loops increases design complexity for electronic systems. Passive directional motion of the fluid using an asymmetric sawtooth microstructure has demonstrated motion along the surface due to viscous forces. The current study explores the sawtooth microstructure in the nucleate boiling regime for a horizontal upward- and downward-facing orientation with varying sawtooth profiles (60°–30° and 75°–15°) in terrestrial gravity conditions as a precursor to the International Space Station (ISS) experiments. A laser powder bed fusion metal additive manufacturing technique was used to fabricate the test surfaces with 250- $\mu \text{m}$ nucleation sites located on the long slopes. For the test surface with cavities spaced 2-mm apart, the wall superheat was ~4 K lower compared to cavities spaced 1 mm apart in the upward-facing orientation. The downward-facing surface induced passive vapor mobility in the direction of the long slope, and a thin liquid film was observed between the vapor mass and microstructure. This liquid film thickness was predicted using a simplified force balance model modified from a prior parabolic flight experiment. The model predicted a uniform liquid film thickness of $49~\mu \text{m}$ for a vapor slug moving at 13.7 mm/s with the input heat flux at 1.25 W/cm2. These terrestrial tests in the adverse orientation suggest that the sawtooth microstructure will successfully induce vapor mobility in upcoming ISS microgravity experiments. Elements of the final flight ampoule, including the cooling zone, the two-stage pressure relief system, and the high-speed imagery setup, are discussed.

Multi-faceted simulation of atomic oxygen erosion of deorbit sail for cleaning space debris

Deorbit sails are used in low earth orbit to reduce the growing amount of orbital debris by rapidly removing satellites at the end of their operational missions. However, in low earth orbit, atomic oxygen (AO) is a potential threat to long-duration exposure facilities. In this work, a multi-faceted simulation for the AO erosion of the Taurus Deorbit Sail was developed to calculate the erosion yield for the membrane material and the undercutting profile at the crease site. The disintegration and erosion detail for the deorbit sail membrane during AO impacts can be studied on an atomic scale. The ReaxFF reaction force field program is used to provide a large enough calculation speed in the size of the system to describe all the chemical reactions of the reaction. The on-orbit flight data from the Materials International Space Station Experiment 2 were used to validate the simulated erosion rate in this study. The defects on the deorbit sails were experimentally measured using a scanning electron microscope and then modeled by the Monte Carlo method. The computational predictions of AO undercutting at the defect sites were validated by experimental data from the NASA long-duration exposure facility. The disintegration of the membrane could be divided into two stages: the physical reaction stage and the chemical reaction stage, after 150 AO collisions the membrane becomes highly volatilized. The maximum undercutting depth for the deorbit drag sail within the predicted 37-month deorbit time was 5.5 μm and 7.5 μm for a defect width of 500 nm and 1 μm, respectively. The undercutting width for a deorbit sail with a width of 500 nm or 1 μm was predicted to be 1.6 μm and 2 μm, respectively, and the breaker was formed at the bottom of the undercutting profile. AO erosion has been studied by researchers in terms of evaluating the effectiveness of protective coatings. This current study, however, seeks to understand the fundamental interactions between AO and creases in the deorbit sail, which provides deeper knowledge than what has been developed so far. The multi-faceted simulation results can provide design references for engineering applications for the deorbit sail.

Space survivability for printed electronics applications

The effects of atomic oxygen (AO) in low earth orbit and the physical effects of space launch on printed materials were studied for aerosol-jet printed Au and Ag through a 6 month exposure period on the Materials International Space Station Experiment (MISSE). RF and DC test platforms were designed for two printed electronics-compatible substrates (Rogers CLTE-XT and Ferro L8) to assess the amount of degradation caused by AO exposure in space. A passivation layer of CORIN-XLS applied over the printed metal traces was also studied for its effectiveness. Both bare and passivated test structures of printed Ag and Au traces were exposed to low earth orbit on the 10th MISSE mission. The effects of AO and the 6 month space exposure on the samples are reported here.

DNA microarray analysis of gene expression of etiolated maize seedlings grown under microgravity conditions in space: Relevance to the International Space Station Experiment “Auxin Transport”

DNA microarray analysis of gene expression of etiolated maize seedlings grown under microgravity conditions in space: Relevance to the International Space Station Experiment “Auxin Transport”

Rotating spherical gap convection in the GeoFlow International Space Station (ISS) experiment

The GeoFlow experiment on the International Space Station was the first experiment to investigate rotating convection in a radial gravitylike force field. This led to the first observation of the existence of global columnar cells in a microgravity environment. The flow structures are identified by a machine learning algorithm and complemented by numerical simulations.

Microarray profile of gene expression in etiolated Pisum sativum seedlings grown under microgravity conditions in space: Relevance to the International Space Station experiment “Auxin Transport”

This paper introduces the use of microarray data technology with Medicago (Medicago truncatula) microarrays to characterize global changes in the transcript abundance of etiolated Alaska pea (Pisum sativum L.) seedlings grown under microgravity (µg) conditions in comparison with those under artificial 1 g conditions on the International Space Station. Of the 44,000 genes of the Medicago microarray platform, more than 25,000 transcripts of pea seedlings were hybridized, suggesting that the microarray platform for Medicago could be useful in the study of gene expression of etiolated pea seedlings grown under µg conditions in space. Gene array data were analyzed according to stringent criteria that restricted the scored genes for specific hybridization values at least twofold. Expression of 1362 and 1558 genes in proximal side (the proximal side) and distal side of the epicotyl to the cotyledons (the distal side), respectively, were highly affected by µg conditions in space. Of the genes analyzed, 407 of 1362 transcripts in the proximal side and 740 of 1558 transcripts in the distal side were expressed at ratios at least twofold. However, in the presence of the auxin transport inhibitor TIBA, 212 of 399 transcripts and 255 of 477 transcripts were expressed at ratios at least twofold as high in the proximal and the distal sides of epicotyls in the seedlings grown under µg conditions, respectively. Based on Venn diagram analysis, 31 transcripts and 24 transcripts were found to commonly increase and decrease, respectively, under µg conditions in space. Venn analysis revealed six auxin-related genes and three water channel AQUAPORIN genes that were responsive to gravity. Among 6 auxin-related genes, the accumulation of transcripts of Auxin-induced protein 5NG4 and Indole-3-acetic acid-amido synthetase GH3.3 tended to increase, and that of Auxin-induced protein, Auxin response factor, SAUR-like auxin-responsive family protein and Auxin response factor tended to decrease under µg conditions, whereas there were no statistic differences between under µg and artificial 1 g conditions. Similarly there were no statistic differences between under µg conditions and artificial 1 g, but the accumulation of NIP3-1 and Plasma membrane intrinsic protein11, and AQUAPORIN1/Tonoplast intrinsic protein tended to increase and decrease, respectively. A possible role of auxin-related genes and AQUAPORIN genes in regulating growth of etiolated pea seedlings grown under µg conditions in space is discussed.

Dissipation of oscillatory contact lines using resonant mode scanning

Moving contact-lines (CLs) dissipate. Sessile droplets, mechanically driven into resonance by plane-normal forcing of the contacting substrate, can exhibit oscillatory CL motions with CL losses dominating bulk dissipation. Conventional practice measures CL dissipation based on the rate of mechanical work of the unbalanced Young’s force at the CL. Typical approaches require measurements local to the CL and assumptions about the “equilibrium” contact angle (CA). This paper demonstrates how to use scanning of forcing frequency to characterize CL dissipation without any dependence on measurements from the vicinity of the CL. The results are of immediate relevance to an International Space Station (ISS) experiment and of longer-term relevance to Earth-based wettability applications. Experiments reported here use various concentrations of a water-glycerol mixture on a low-hysteresis non-wetting substrate.

Coherent Capillary Wave Structure Revealed by ISS Experiments for Spontaneous Nozzle Jet Disintegration

A series of International Space Station (ISS) experiments were conducted to observe the disintegration feature of a water jet issued from an orifice/nozzle into atmospheric air. The purpose was to validate our proposal that any laminar liquid jet can spontaneously disintegrate by its own self-destabilizing loop formed along the jet. This paper reports the experiment results focusing on a water jet issued from a nozzle with a radius 0.4 mm and length 120 mm, in which the parabolic velocity profile relaxes toward that of a plug flow along the jet. As predicted in our proposal, the nozzle jet had a two-valued breakup distance in a certain jet issue speed range and exhibited hysteresis behaviors, indicating that the jet disintegration state is determined by past jet disintegration history. Analyses of video images suggest the establishment of a coherent capillary wave structure in the steady jet disintegration state. New fundamental theories were developed to examine the underlying physics involved. The short-length breakup mode was confirmed to essentially follow the same self-destabilizing mechanism as that of the plug flow jet, in which the upstream propagating capillary wave produced by the release of surface energy due to jet tip contraction or nonlinear unstable wave growth is reflected at the orifice, and becomes the unstable wave responsible for jet disintegration. In the long-length breakup mode, the velocity profile relaxation plays a role equivalent to an orifice and the average nozzle jet length is expressed as the sum of the velocity profile relaxation length and average orifice jet length at large jet issue speeds. This paper focuses on the coherent capillary wave structure of long-length breakup mode.

Altered localisation of ZmPIN1a proteins in plasma membranes responsible for enhanced-polar auxin transport in etiolated maize seedlings under microgravity conditions in space.

In the International Space Station experiment 'Auxin Transport', polar auxin transport (PAT) in shoots of etiolated maize (Zea mays L. cv. Golden Cross Bantam) grown under microgravity in space was substantially enhanced compared with those grown on Earth. To clarify the mechanism, the effects of microgravity on expression of ZmPIN1a encoding essential auxin efflux carrier and cellular localisation of its products were investigated. The amounts of ZmPIN1a mRNA in the coleoptiles and the mesocotyls in space-grown seedlings were almost the same as those in 1 g-grown seedlings, but its products were not. Immunohistochemical analysis with anti-ZmPIN1a antibody revealed a majority of ZmPIN1a localised in the basal side of plasma membranes of endodermal cells in the coleoptiles and the mesocotyls, and in the basal and lateral sides of plasma membranes in coleoptile parenchymatous cells, in which it directed towards the radial direction, but not towards the vascular bundle direction. Microgravity dramatically altered ZmPIN1a localisation in plasma membranes in coleoptile parenchymatous cells, shifting mainly towards the vascular bundle direction. These results suggest that mechanism of microgravity-enhanced PAT in maize shoots is more likely to be due to the enhanced ZmPIN1a accumulation and the altered ZmPIN1a localisation in parenchymatous cells of the coleoptiles.

Space Station conditions are selective but do not alter microbial characteristics relevant to human health

The International Space Station (ISS) is a unique habitat for humans and microorganisms. Here, we report the results of the ISS experiment EXTREMOPHILES, including the analysis of microbial communities from several areas aboard at three time points. We assess microbial diversity, distribution, functional capacity and resistance profile using a combination of cultivation-independent analyses (amplicon and shot-gun sequencing) and cultivation-dependent analyses (physiological and genetic characterization of microbial isolates, antibiotic resistance tests, co-incubation experiments). We show that the ISS microbial communities are highly similar to those present in ground-based confined indoor environments and are subject to fluctuations, although a core microbiome persists over time and locations. The genomic and physiological features selected by ISS conditions do not appear to be directly relevant to human health, although adaptations towards biofilm formation and surface interactions were observed. Our results do not raise direct reason for concern with respect to crew health, but indicate a potential threat towards material integrity in moist areas.

Gravity-regulated localization of PsPIN1 is important for polar auxin transport in etiolated pea seedlings: Relevance to the International Space Station experiment

To clarify the mechanism of gravity-controlled polar auxin transport, we conducted the International Space Station (ISS) experiment “Auxin Transport” (identified by NASA's operation nomenclature) in 2016 and 2017, focusing on the expression of genes related to auxin efflux carrier protein PsPIN1 and its localization in the hook and epicotyl cells of etiolated Alaska pea seedlings grown for three days in the dark under microgravity (μg) and artificial 1 g conditions on a centrifuge in the Cell Biology Experiment Facility (CBEF) in the ISS, and under 1 g conditions on Earth. Regardless of gravity conditions, the accumulation of PsPIN1 mRNA in the proximal side of epicotyls of the seedlings was not different, but tended to be slightly higher as compared with that in the distal side. 2,3,5-Triiodobenzoic acid (TIBA) also did not affect the accumulation of PsPIN1 mRNA in the proximal and distal sides of epicotyls. However, in the apical hook region, TIBA increased the accumulation of PsPIN1 mRNA under μg conditions as compared with that under artificial 1 g conditions in the ISS. The accumulation of PsPIN1 proteins in epicotyls determined by western blotting was almost parallel to that of mRNA of PsPIN1. Immunohistochemical analysis with a specific polyclonal antibody of PsPIN1 revealed that a majority of PsPIN1 in the apical hook and subapical regions of the seedlings grown under artificial 1 g conditions in the ISS localized in the basal side (rootward) of the plasma membrane of the endodermal tissues. Conversely, in the seedlings grown under μg conditions, localization of PsPIN1 was greatly disarrayed. TIBA substantially altered the cellular localization pattern of PsPIN1, especially under μg conditions. These results strongly suggest that the mechanisms by which gravity controls polar auxin transport are more likely to be due to the membrane localization of PsPIN1. This physiologically valuable report describes a close relationship between gravity-controlled polar auxin transport and the localization of auxin efflux carrier PsPIN1 in etiolated pea seedlings based on the μg experiment conducted in space.

Photochemistry on the Space Station-Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station.

Some microarray-based instruments that use bioaffinity receptors such as antibodies or aptamers are under development to detect signatures of past or present life on planetary bodies. Studying the resistance of such instruments against space constraints and cosmic rays in particular is a prerequisite. We used several ground-based facilities to study the resistance of aptamers to various types of particles (protons, electrons, neutrons, and carbon ions) at different energies and fluences. We also tested the resistance of aptamers during the EXPOSE-R2 mission outside the International Space Station (ISS). The accumulated dose measured after the 588 days of this mission (220 mGy) corresponds to the accumulated dose that can be expected during a mission to Mars. We found that the recognition ability of fluorescently labeled aptamers was not significantly affected during short-term exposure experiments taking into account only one type of radiation at a time. However, we demonstrated that the same fluorescent dye was significantly affected by temperature variations (-21°C to +58°C) and storage throughout the entirety of the ISS experiment (60% of signal loss). This induced a large variability of aptamer signal in our analysis. However, we found that >50% of aptamers were still functional after the whole EXPOSE-R2 mission. We conclude that aptamer-based instruments are well suited for in situ analysis on planetary bodies, but the detection step requires additional investigations.