A single loop Pulsating Heating Pipe (PHP) experimental setup was developed for non-invasive measurement of the local heat transfer coefficient and the mass flow along the PHP evaporator. These measurements are valuable to validate modeling tools used in the design of PHP. A two-step differential heat input method was used for measurement of mass flow and the results were compared with the optical and theoretical results. The conditions tested in the PHP were 50, 60 and 70% filling ratio, R134a as working fluid, internal diameter of 2 mm, saturation temperature of between 30 to 40 °C, inclination angles from 30 to 90° (vertical bottom heat mode) and heat loads on the evaporator from 10 up to 68 W. The results indicate a high performance in heat transfer of the PHP, with minimum internal thermal resistance values close to 0.008 K/W as well as local heat transfer coefficients greater than 10 kW/m2K for observed values of mass velocities between 60 and 140 kg/m2s. The results were compared with correlations from the literature and these were able to predict the results with a 20% average error margin, suggesting many similarities with convective boiling mechanisms.