Intermittent Pattern Research Articles

Noninvasive modulation of the hippocampal-entorhinal complex during spatial navigation in humans.

Because of the depth of the hippocampal-entorhinal complex (HC-EC) in the brain, understanding of its role in spatial navigation via neuromodulation was limited in humans. Here, we aimed to better elucidate this relationship in healthy volunteers, using transcranial temporal interference electric stimulation (tTIS), a noninvasive technique allowing to selectively neuromodulate deep brain structures. We applied tTIS to the right HC-EC in either continuous or intermittent theta-burst stimulation patterns (cTBS or iTBS), compared to a control condition, during a virtual reality-based spatial navigation task and concomitant functional magnetic resonance imaging. iTBS improved spatial navigation performance, correlated with hippocampal activity modulation, and decreased grid cell-like activity in EC. Collectively, these data provide the evidence that human HC-EC activity can be directly and noninvasively modulated leading to changes of spatial navigation behavior. These findings suggest promising perspectives for patients suffering from cognitive impairment such as following traumatic brain injury or dementia.

Influence of momentum ratio on heat release rate and noise of co/counter-swirl non-premixed diluted CO/H2 flames

Understanding co/counter-swirl or twin-swirl flames remains challenging due to the complex interaction of two swirling streams. In the present study, we investigate the heat release features of non-premixed co/counter-swirl syngas/air flames and their’ influence on combustor noise in a ∼20 kW combustor using simultaneous high-speed OH*-chemiluminescence (5 kHz) and microphone measurement (50 kHz) by varying the momentum ratio (M) from 0.4 to 0.95. Furthermore, the velocity field is examined using a low-speed two-dimensional particle image velocimetry (2D-PIV, frequency = 7 Hz). For all studied momentum ratios, the frequency spectra of noise measurements for both co/counter-swirl flames consistently exhibit a dominant frequency (∼285 Hz), close to the fundamental axial mode of the combustor. A further analysis using spectrograms, phase spaces, and recurrence plots reveals intermittent patterns in noise measurements, featuring periodic (P) and aperiodic regions (A). In periodic regions (P), noise synchronizes with the global fluctuation of the heat release rate as observed in chemiluminescence. Along with global fluctuation, the chemiluminescence also reveals a rotational component of heat release rate with distinct frequencies for co and counter-swirl configurations. This rotational motion possibly originated from a precessing vortex core (PVC) as indicated by the zig-zag arrangements of vortices in the inner shear layer. Furthermore, the impact of M on global fluctuation and rotational motion has been investigated using the frequency spectrum of OH*-intensity and the distribution of peaks in noise measurement. The global fluctuation is found to be suppressed when M increases while the rotational component becomes prominent at higher M. Therefore, the study elucidates the co-existence of global fluctuation and rotational motion and how these motions evolve with the varying momentum ratio (M), thus enhancing the understanding of combustion characteristics of the complex twin-swirl (co/counter-swirl) flames.

Dynamic alterations and ecological implications of rice rhizosphere bacterial communities induced by an insect-transmitted reovirus across space and time

BackgroundCereal diseases caused by insect-transmitted viruses are challenging to forecast and control because of their intermittent outbreak patterns, which are usually attributed to increased population densities of vector insects due to cereal crop rotations and indiscriminate use of pesticides, and lack of resistance in commercial varieties. Root microbiomes are known to significantly affect plant health, but there are significant knowledge gaps concerning epidemics of cereal virus diseases at the microbiome-wide scale under a variety of environmental and biological factors.ResultsHere, we characterize the diversity and composition of rice (Oryza sativa) root-associated bacterial communities after infection by an insect-transmitted reovirus, rice black-streaked dwarf virus (RBSDV, genus Fijivirus, family Spinareoviridae), by sequencing the bacterial 16S rRNA gene amplified fragments from 1240 samples collected at a consecutive 3-year field experiment. The disease incidences gradually decreased from 2017 to 2019 in both Langfang (LF) and Kaifeng (KF). BRSDV infection significantly impacted the bacterial community in the rice rhizosphere, but this effect was highly susceptible to both the rice-intrinsic and external conditions. A greater correlation between the bacterial community in the rice rhizosphere and those in the root endosphere was found after virus infection, implying a potential relationship between the rice-intrinsic conditions and the rhizosphere bacterial community. The discrepant metabolites in rhizosphere soil were strongly and significantly correlated with the variation of rhizosphere bacterial communities. Glycerophosphates, amino acids, steroid esters, and triterpenoids were the metabolites most closely associated with the bacterial communities, and they mainly linked to the taxa of Proteobacteria, especially Rhodocyclaceae, Burkholderiaceae, and Xanthomonadales. In addition, the greenhouse pot experiments demonstrated that bulk soil microbiota significantly influenced the rhizosphere and endosphere communities and also regulated the RBSDV-mediated variation of rhizosphere bacterial communities.ConclusionsOverall, this study reveals unprecedented spatiotemporal dynamics in rhizosphere bacterial communities triggered by RBSDV infection with potential implications for disease intermittent outbreaks. The finding has promising implications for future studies exploring virus-mediated plant-microbiome interactions.2AL1xEb7k2nunVBmbuNCsVVideo

Investigation on intermittent flow characteristics in horizontal pipe by visualization measurement method

Accurate measurement and prediction of the intermittent flow characteristics in horizontal pipes is important for constructing multiphase flow models and ensuring pipe flow safety. In this paper, a quantitative image post-processing technique for intermittent flow characteristics based on gray histogram image similarity is proposed, which can realize the measurement of slug frequency. In addition, the technique also has the ability to classify a large number of images, and can quickly find the elongated bubble head, liquid film area and liquid slug area of intermittent flow. On the basis of this technique, combined with image post-processing methods such as gas–liquid interface feature analysis, a set of intermittent flow image processing technique with perfect route is formed. Based on this post-processing technique, the similarity image oscillation trajectories of plug flow and slug flow are obtained. There are differences in the similarity image oscillation trajectories of the two intermittent sub-flow patterns, and the similarity image of the plug flow has an obvious platform period and trailing rising line, which can be used as a basis for the classification of the two intermittent sub-flow patterns. A correlation for predicting the slug frequency of intermittent sub-flow patterns is developed. The accuracy of this slug frequency prediction correlation can be improved by about 10 % compared to not dividing the sub-flow patterns. When the mixture Froude number Frm is less than 5.0, the radial position of the elongated bubble head decreases linearly as the Frm increases. When the Frm is greater than 5.0, the elongated bubble head oscillates near the middle of the pipe. Prediction correlations for the radial position of the elongated bubble head and the slug velocity are established separately, and the maximum error is ± 10 %. The modified mixed Froude number is proposed, and based on this, a new prediction model for the transition from plug flow to slug flow is established.

Endomorphin-2 (Endo2) and substance P (SubP) co-application attenuates SubP-induced excitation and alters frequency plasticity in neonatal rat in vitro preparations

Substance P (SubP) and endomorphin-2 (Endo2) are co-localized presynaptically in vesicles of neurons adjacent to inspiratory rhythm-generating pre-Botzinger Complex (preBotC) neurons but the effects of co-released SubP and Endo2 on respiratory motor control are not known. To address this question, SubP alone or a combination of SubP and Endo2 (SubP/Endo2) were bath-applied in a sustained (15-min) or intermittent (5-min application, 5-min washout, x3) pattern at 10–100 nM to neonatal rat brainstem-spinal cord preparations. During neuropeptide application, SubP/Endo2 co-applications generally attenuated SubP-induced increases in burst frequency and decreases in burst amplitude. With respect to frequency plasticity (long-lasting increase in burst frequency 60 min post-neuropeptide application), SubP-induced frequency plasticity was increased with sustained SubP/Endo2 co-applications at 20 and 100 nM. Intermittent SubP/Endo2 co-applications tended to decrease the level of frequency plasticity induced by intermittent SubP alone applications. SubP/Endo2 co-applications revealed potentially new functions for neurokinin-1 (NK1R) and mu-opioid (MOR) receptors on respiratory rhythm-generating medullary neurons.

Grid-integrated solutions for sustainable EV charging: a comparative study of renewable energy and battery storage systems

IntroductionThe integration of electric vehicles (EVs) into the power network challenges the 1) grid capacity, 2) stability, and 3) management. This is due to the 1) increased peak demand, 2) infrastructure strain, and 3) intermittent charging patterns. Previous studies lack comprehensive integration of renewable energy and battery storage with EV charging.MethodsTo address these challenges, this study explores the effectiveness of incorporating renewable energy resources (RERs) and battery energy storage systems (BESS) alongside the traditional grid. The proposed study utilizes the HOMER Grid® and conducted a comprehensive analysis.ResultsThe proposed study compares two grid integrated scenarios: 1) Case-1 (grid and photovoltaic (PV) systems), and 2) Case-2 (grid, PV systems, and BESS). Both these scenarios are compared against a Base case relying solely on grid power. The evaluation employed techno-economic analysis while focusing on 1) net present cost (NPC), 2) cost of energy, and 3) annualized savings. Additionally, the proposed study analyzed 4) seasonal variations in EV charging demand, 5) grid interactions, 6) PV production, and 7) the operation of BESS in both summer and winter. The comparative analysis reveals that the Base case incurs a net present cost (NPC) of $546,977 and a cost of energy (COE) of $0.354 per kWh. In contrast, Case-1, which integrates a 100 kW PV system, shows a significantly lower NPC of -$122,962 and a reduced COE of -$0.043 per kWh, with annualized savings of $61,492. Case-2, incorporating both the 100 kW PV system and a BESS with a capacity of 9.8 kWh, has a higher NPC of $309,667 but a COE of $0.112 per kWh and provides annual savings of $51,233 compared to the Base case.DiscussionSeasonal analysis highlights that Case-2 achieves the lowest carbon emissions in summer, ranging from 2.0 to 2.5 tons, while Case-1 shows the lowest emissions in winter, ranging from 3.2 to 3.4 tons. This model 1) reduces operational costs, 2) minimizes carbon emissions, while 3) making it compelling for future energy systems in increasing EV adoption.

Does intermittent water supply influence beverage consumption patterns among adolescents and adults in Mexico? A nationally representative, cross-sectional analysis

Does intermittent water supply influence beverage consumption patterns among adolescents and adults in Mexico? A nationally representative, cross-sectional analysis

A Critical study of Somanathi Tamra Bhasma- A mercuro-arsenical formulation

Background: Tamra Bhasma has been mentioned in classics for its wider therapeutic utility, at the same time it is reported as poison as or more than that if not processed properly as per classical guidelines. To emphasize its toxic potential, Ashtamahadoshas (eight major ill effects) have been quoted in classics. Numerous methods preparation of Tamra Bhasma was available in classics of Rasashastra among them Somanathi Tamra Bhasma (STB) is specialized method. Preparation of Somanathi Tamra Bhasma involves less complex pharmaceutical procedures, duration, human efforts than Tamra Bhasma prepared by multiple incineration cycles. Considering this multiple research works has been carried out on Somanathi Tamra Bhasma. Hence present study is planned to compile available research works and published research article and provide brief information of pharmaceutical, analytical, pharmacological and clinical studies. Material and methods: Research works carried out at different institutes and published articles were compiled and screened to gather information on literary review, pharmaceutical, pharmacological and clinical aspects of Somanathi Tamra Bhasma. Results: Total four dissertation works and seven published research articles were found. Among four dissertation works two were related to pharmaceutical, three were related to pharmacological study and two were related to clinical study. Among published research articles one is related to literary review, two are related to pharmaceutical, four were related to pharmacological study. Conclusion: Somanathi Tamra Bhasma can be prepared with 3-36 hours of duration with intermittent heating pattern (highest temperature 615°C) by both traditional heating method and Kupipakwa method. In analytical study it is found to be mixture of copper sulphide (CuS) and copper oxide (CuO). Pharmacologically it is found safer after Amritikarana upto repeated dose 90-days oral toxicity study at therapeutic dose with appropriate adjuvant. Clinically it is found more effective than Tamra Bhasma in Shwasa, Grahani and Medoroga.

Search for a critical point of strongly-interacting matter in central 40\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$^{40}$$\\end{document}Ar + 45\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$^{45}$$\\end{document}Sc collisions at 13 A–75 A GeV/c beam momentum

The critical point of strongly interacting matter is searched for at the CERN SPS by the NA61/SHINE experiment in central 40\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$^{40}$$\\end{document}Ar + 45\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$^{45}$$\\end{document}Sc collisions at 13 A, 19 A, 30 A, 40 A, and 75 A GeV/c. The dependence of the second-order scaled factorial moments of proton multiplicity distributions on the number of subdivisions in transverse momentum space is measured. The intermittency analysis uses statistically independent data sets for every subdivision in transverse and cumulative-transverse momentum variables. The results obtained do not indicate the searched intermittent pattern. An upper limit on the fraction of correlated protons and the intermittency index is obtained based on a comparison with the Power-law Model.

Effect of micro and nanobubble size and concentration on membrane fouling: Strategies for energy-saving and ultrafiltration efficiency enhancement

The impact of micro and nanobubbles (MNBs) on preventing membrane fouling in various filtration techniques has been effectively demonstrated. However, there remains a gap in understanding the influence of their size and concentration on membrane fouling. Moreover, the substantial energy demand for MNBs-aided filtration processes hinders the wide adoption of this technology on a global scale. This study controlled MNB size and concentration by optimizing the release pressure of air-saturated water and introducing intermittent operation schemes. At 0.35 MPa, the MNBs averaged 91 μm and 117.9 nm with concentrations of 3.27E+3 and 6.85E+6 particles mL−1. Increasing the pressure to 0.60 MPa reduced the average size to 31 μm and 119.9 nm. Concentrations to 6.43E+5 and 9.40E+6 particles mL−1. Bubbles released at 0.60 MPa reduced TMP from −0.0109 MPa attained with 0.35 MPa bubbles to −0.0052 MPa making a 70 % decrease. Comparing continuous and optimal intermittent release patterns (30 min) revealed marginal differences: normalized transmembrane pressure (4 %), cleaning efficiency (9 %), and rejection efficiency (2.4 %). Our approach enhances membrane filtration efficiency, maintains performance, reduces energy consumption, and advances sustainable filtration technologies encouraging global adoption.

Investigation of landscape risk factors for the recent spread of varroa mite (Varroa destructor) in European honeybee (Apis mellifera) colonies in New South Wales, Australia.

In June 2022, an exotic pest of the European honeybee (Apis mellifera), the varroa mite (Varroa destructor), was detected in surveillance hives at the Port of Newcastle, New South Wales (NSW). Previously, Australia remained the only continent free of the varroa mite. In September 2023, the National Management Group decided to shift the focus of the response from eradication to management. It is estimated that the establishment of varroa mite in Australia could lead to more than $70 million in losses each year due to greatly reduced pollination services. Currently, there are no reported studies on the epidemiology of varroa mite in NSW because it is such a recent outbreak, and there is little knowledge of the factors associated with the presence of V. destructor in the Australian context. We sourced publicly available varroa mite outbreak reports from June 22 to December 19, 2022, to determine if urbanization, land use, and distance from the incursion site are associated with the detection of varroa mite infestation in European honeybee colonies in NSW. The outcome investigated was epidemic day, relative to the first detected premises (June 22, 2022). The study population was comprised of 107 premises, which were declared varroa-infested. The median epidemic day was day 37 (July 29, 2022), and a bimodal distribution was observed from the epidemic curve, which was reflective of an intermittent source pattern of spread. We found that premises were detected to be infected with varroa mite earlier in urban areas [median epidemic day 25 (July 17, 2022)] compared to rural areas [median epidemic day 37.5 (July 29, 2022)]. Infected premises located in areas without cropping, forests, and irrigation were detected earlier in the outbreak [median epidemic days 23.5 (July 15, 2022), 30 (July 22, 2022), and 15 (July 7, 2022), respectively] compared to areas with cropping, forests, and irrigation [median epidemic days 50 (August 11, 2022), 43 (August 4, 2022), and 47 (August 8, 2022), respectively]. We also found that distance from the incursion site was not significantly correlated with epidemic day. Urbanization and land use are potential factors for the recent spread of varroa mite in European honeybee colonies in NSW. This knowledge is essential to managing the current varroa mite outbreak and preventing future mass varroa mite spread events.

Enhancing Weather Forecasting Integrating LSTM and GA

Several sectors, such as agriculture and renewable energy systems, rely heavily on weather variables that are characterized by intermittent patterns. Many studies use regression and deep learning methods for weather forecasting to deal with this variability. This research employs regression models to estimate missing historical data and three different time horizons, incorporating long short-term memory (LSTM) to forecast short- to medium-term weather conditions at Quinta de Santa Bárbara in the Douro region. Additionally, a genetic algorithm (GA) is used to optimize the LSTM hyperparameters. The results obtained show that the proposed optimized LSTM effectively reduced the evaluation metrics across different time horizons. The obtained results underscore the importance of accurate weather forecasting in making important decisions in various sectors.

Analysis of spatial pattern of water supply continuity in an Indian metropolis: a case study of Hyderabad

ABSTRACT Water supply continuity is a crucial indicator of service efficiency. Multiple South Asian cities have Intermittent Water Supply (IWS). Water supply equity in cities depends on the spatial patterns of intermittency. The present study used Kernel Density Estimation (KDE) to investigate the spatial pattern of water supply intermittency for 3071 localities of Hyderabad, India. Further, the relationship between population density and supply continuity has been examined in 146 wards of Hyderabad using Spatial Autocorrelation and Geographically Weighted Regression (GWR). Spatial Autocorrelation defines two distinct clusters of central wards for high continuity (12 wards) and population density (19 wards). However, the ward clusters are different for continuity and density, with only three central wards (2.06% of all wards) being common in both the high-high clusters. The results highlight that the water supply continuity in Hyderabad has minimal consideration for population density, resulting in a spatially inequitable water supply.

Morphological identification of brugada type 1 pattern using loop recorder: a feasibility study

Abstract Introduction The presence of spontaneous type 1 Brugada pattern (BP1) is associated with an elevated risk of arrhythmic events. Diagnosis of this condition is often underestimated due to its intermittent nature. While periodic ambulatory ECG monitoring can distinguish patients with pharmacologically induced patterns from those with spontaneous patterns, the diagnostic capabilities of this method are limited. Implantable cardiac monitors (ICM), designed to continuously record heart rhythm, may offer higher sensitivity in detecting patients with intermittent patterns. However, the ICM's ability to morphologically identify the ST segment elevation associated with BP1 remains uncharted. Purpose This study aimed to assess the ICM's capacity to morphologically identify the ST segment elevation associated with BP1. Methods We enrolled all patients who underwent ajmaline testing (AT) between November 2022 and September 2023 in our centre. For positive patients, the external simulator of a loop recorder was utilized to evaluate four positions (I-II left intercostal space at a 45° tilt, II-III left intercostal space at a 45° tilt, II-III right parasternal intercostal space, and II-III left parasternal intercostal space). These positions were selected to capture the trace with the most significant ST segment elevation at the J point during the peak positivity of the ECG during the ajmaline test. Measurements from the recorded traces were acquired using dedicated software. Results Out of 50 patients, 21 (male-to-female ratio 1.62:1, mean age 43.4±13.1 years) had a positive AT result. Seventeen patients exhibited ST segment elevation in the simulator trace during the highest ajmaline dose. Among these patients, the optimal recording position was in the I-II left intercostal space at a 45° tilt in 66.7% of cases. The median ST segment elevation recorded by the simulator was 0.9 mm (interquartile range 0.6-1.1 mm). Notably, there was no observed correlation between the extent of ST segment elevation in the ECG and that recorded by the simulator trace (Pearson linear coefficient = 0.09). Conclusions ICM may offer the potential to record BP1, a finding that holds significant diagnostic and prognostic implications for asymptomatic patients with pharmacologically induced BP1. Further research is needed to develop useful programming algorithms for the automatic recording of this pattern.

Architecture and history of uranium-bearing Palaeocene–Eocene strata deposited on the eastern margin of the Peri-Tethys (Chu-Sarysu Basin, south Kazakhstan)

Deciphering the facies and architecture of alluvial successions provides invaluable insights into the interplay of tectonics, climate, eustasy, and autogenic processes affecting terrestrial sedimentary systems. The stratigraphic response of fluvial systems to variations in discharge and sediment-supply regimes is now well-understood and is tied to changes in climate, precipitation patterns, or sediment sources. The uraniferous Chu-Sarysu Basin in south Kazakhstan occupies the tectonically stable Turan Platform on the eastern margin of the Peri-Tethys, and the study of its Palaeocene–Eocene sedimentary fill offers an opportunity to unravel eustatic and climatic controls that drove the architecture of reservoirs hosting economically important deposits. The stratigraphic succession comprises two multistorey, laterally extensive, sheet-like sandstone bodies floored by prominent erosion surfaces and interpreted as the deposits of channel belts. These packages are interstratified with floodplain, coastal-wetland, and marine-embayment complexes reflecting major extra-channel belt avulsions and marginal-marine incursions on the low-gradient alluvial plain. The stratal architecture was controlled by changes in accommodation likely induced by well-documented sea-level changes in the Peri-Tethys during the Palaeogene, which permitted the development of a chronostratigraphic framework. Eustatic variations culminated in the widespread flooding of the Turan Platform in the middle Eocene, reflected by transgressive lags above a wave ravinement surface capping terrestrial and marginal-marine deposits. Marked changes in fluvial facies, floodplain styles, and inferred channel planforms between Palaeocene and Eocene strata suggest a climatic overprint on river discharge and sedimentation. Upper Palaeocene meandering channel deposits encased in well-drained floodplain strata are indicative of perennial discharge under a semi-arid climate, whereas lower Eocene low-sinuosity channel fills, displaying evidence for transcritical flows and abundant in-situ vegetation, point to intermittent runoff patterns consistent with a humid and seasonal climate. An evolution in atmospheric moisture at the Palaeocene–Eocene boundary from arid to humid conditions has been reported across the Tethys region, and linked to global climatic perturbations of the Palaeocene–Eocene Thermal Maximum.

Kinematics of Two Special Endurance Trials: A Methodological Contribution to 400-m Performance.

This study aimed to determine changes in the kinematics of sprint steps based on progressive muscular fatigue during high-intensity 350-m and 500-m trials. Twelve elite healthy male 400-m sprinters with a minimum of six years of regular sprint training experience were recruited. They were divided into two groups for the experiment: a 350-m and a 500-m trial group. Time and kinematics of sprinting step motion for specific segments, i.e., starting to final stages of each trial, were obtained using the Opto Jump-Microgate optical measurement system. The starting phase of each sprint was defined as the section without muscular fatigue (noF), and the final phase was the sprint under muscular fatigue (onF). Each last 25 m of the 50-m evaluated section containing ten complete running steps was selected for detailed statistical analysis. Various patterns of temporal and spatial variables of sprinting efforts were observed between 350-m and 500-m trials. Each trial result was influenced by significant individual changes (p < 0.05). All variables indicated that the two distances differed significantly in terms of running kinematics. This was confirmed by significant differences in the mean step frequency (p < 0.001), which presented a difference of 11.75%, and the mean step speed (p < 0.001). As a result of these changes, a hierarchical intermittent endurance training pattern was defined. The research concluded that special endurance (intermittent sprints) based on 350 m differed significantly in kinematics from sprints over 500 m. Therefore, it should be assumed that the distance of 350 m is more similar in its kinematics to the 400-m competition. This should encourage coaches and athletes to apply a 350-m distance in training developing special endurance, especially in the pre-competitive and competitive periods.

Historical biogeography of the Saharan horned viper enlightens past dynamics of hyperarid desert habitats

Hyperarid habitats comprise the most dominant and harsh portion of the Sahara Desert, yet how local biotas have responded to paleoclimatic shifts and landscape heterogeneity remains unclear. Here, we assess the historical biogeography of Cerastes cerastes, a conspicuous but poorly known vertebrate inhabiting the hyperarid Sahara, to understand the links between intermittent and stable climatic suitability and patterns of genetic diversity and differentiation in these extreme environments. We combine phylogeographic and ecological modelling tools using a comprehensive species dataset, which includes DNA samples (n = 62) and occurrence records (n = 557) covering most of its range. We show that paleoclimatic oscillations were major evolutionary and biogeographic drivers in C. cerastes, which exhibits different levels of intra-specific diversity and allopatric lineage structuration across the desert. High lineage richness in the north-western Sahara contrasts with an overall lack of genetic diversity throughout the central and eastern Sahara. Climatic refugia along the desert's periphery and around mountain patches harbour single endemic lineages, stressing the links between stable climatic conditions and population isolation and divergence. Increasing climatic suitability during historical arid phases prompted widespread connectivity across the desert. This study lays the groundwork for better understanding the historical dynamics of the hyperarid Sahara.

Impact of heating strategies towards energy-efficient buildings

Mitigating climate change and its impact requires intensive efforts to reduce greenhouse gas emissions, transition to renewable energy sources, and implement sustainable practices. Intermittent heating is considered a sustainable and effective way to achieve energy savings compared with continuous heating mode. However, recent studies have found that energy savings from intermittent heating depend on several factors, namely the building envelope solution. Therefore, this work intends to fill the research gap by comparing the energy efficiency and thermal performance of different construction systems, using two real-scale test cells, one built with a Light Steel Framing (LSF) solution and the other with concrete and Hollow Brick Masonry (HBM) solution. An experimental monitoring campaign was performed during the heating season, aimed at analysing the energy consumption, the responsiveness of the test cells to the heating system, and the influence of the outdoor climate on energy consumption, considering different heating strategies (intermittent patterns and continuous). In general, the LSF test cell registered lower energy consumption when compared to the HBM test cell. However, the LSF test cell presented significant fluctuations of the indoor temperature, confirming that it is more susceptible to the outdoor climate conditions. This research concluded that a system with lower thermal inertia has superior energy saving potential.

Diaphragm Pacing Elicits Ventilatory Plasticity in a Rodent Model of Spinal Cord Injury

Cervical spinal cord injury (cSCI) often results in respiratory impairments requiring the use of chronic mechanical ventilation (MV). Diaphragm pacing (DP) is an emerging clinical intervention designed to combat the deleterious effects of chronic MV by electrically stimulating the diaphragm muscle to maintain ventilation. Anecdotal clinical evidence suggests a rehabilitative benefit of DP; however, current clinical practice is limited by a lack of controlled investigations. Thus, the purpose of this ongoing study was to determine if daily DP could be used as a rehabilitative tool following cSCI. We hypothesized that following cSCI, DP would 1) induce a long-lasting increase in tidal volume 60 minutes after stimulation, and 2) result in sustained improvements in tidal volume after four days of daily DP in awake, behaving rats. To test these hypotheses, we implanted custom electromyography electrodes in the mid-costal region of the left and right hemidiaphragms of male Sprague-Dawley rats. One week later, all animals received a left C2 hemisection (C2Hx; n=14) and a subset also received a left C4-6 dorsal rhizotomy (n=3). One week after injury, rats received stimulation on the paralyzed hemidiaphragm (biphasic 30Hz, 0.8mA, 80 μs pulse width) in phase with breathing (Pacing n=5, Rhizotomy n=3), or served as controls (n=6). We delivered DP to animals within plethysmography chambers in an intermittent pattern (5 minutes on/5 minutes off) for 1 hour/day over 4 consecutive days. Using whole body plethysmography, we recorded ventilation before, during, and for 60 minutes after to examine the acute effects of DP. Additionally, we measured ventilation during normoxia (21% FiO2) and respiratory challenge (10.5% FiO2, 7% FiCO2) before and after C2Hx, and 24 hours after four days of DP to assess the rehabilitative potential of pacing. DP increased tidal volume (% change from pre-pacing) compared to control animals during stimulation (P =0.021). Animals receiving DP displayed an elevated tidal volume compared to controls for at least 60 minutes after DP (P=0.015). When compared to control animals 24 hours after the completion of the pacing protocol, DP animals displayed an increased tidal volume (mL/breath/100g) during both normoxia (P =0.006) and respiratory challenge (P =0.019). In contrast, animals pre-treated with rhizotomy showed a similar tidal volume (mL/breath/100g) compared to controls (normoxia: P=0.597; respiratory challenge: P=0.191), suggesting that DP-induced ventilatory plasticity requires phrenic afferents. Taken together, our data suggest that diaphragm pacing has rehabilitative potential and elicits functional respiratory improvements in rodents following C2Hx. Acknowledgements: This work was supported by funding from the National Institute of Health, grant numbers: R00 HL143207-01 (KAS), UL1TR001436 (TCH) and the APTA Acute Care Physical Therapy Seed Grant (TCH). This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Temporal Forecast of Maize Production in Tanzania: An Autoregressive Integrated Moving Average Approach

This paper aims to model and forecast maize production in Tanzania, emphasizing its crucial contribution to the agricultural sector. Utilizing secondary data on annual maize production in Tanzania from 1961 to 2021, measured in tonnes and sourced from the FAO database, the study employs statistical techniques such as unit root tests, the ARIMA model, and the Ljung-Box test for a comprehensive analysis. The optimal model for forecasting maize production in Tanzania is identified as ARIMA (5,1,5), and the adequacy assessment confirms its effectiveness in predicting maize production values. The ten-year forecast reveals an intermittent pattern, offering valuable insights into anticipated trends. While not explicitly developing an existing theory, this research enhances the understanding of maize production dynamics in Tanzania through the application of advanced statistical methods. Policymakers, agricultural stakeholders, and researchers can leverage these findings to enhance agricultural productivity and food security in Tanzania.