Time Interaction Research Articles

A resiliency intervention adapted for older women with HIV: Results from a pilot randomized controlled trial in the northeastern US.

Aging with HIV often results in psychosocial and health-related challenges for women; however, no resiliency interventions exist for older women with HIV (WWH). WWH aged ≥50 were randomized to 10 group sessions of an adapted resiliency intervention or time-matched supportive psychotherapy. Assessments were conducted at three timepoints. Feasibility and acceptability metrics were defined a priori; differences in resilience, stress coping, anxiety, and depression across timepoints were assessed. Overall, 44 WWH enrolled; participants were 58 years old on average, and 56.4% identified as Black/African American. Among those who attended any sessions, all feasibility metrics were met, and the intervention was acceptable. The interaction of study arm and time was associated with significant decreases in depression and a trend toward significant decreases in anxiety. The intervention was not associated with changes in resilience or stress coping. Adjusting delivery modality may further reduce barriers to attendance, improving feasibility and clinical outcomes.

Potassium and harvest time interaction effect on alfalfa production and profitability

Potassium and harvest time interaction effect on alfalfa production and profitability

Effectiveness of a needs-tailored nurse-led recovery program for community-dwelling people with schizophrenia: a cluster-randomized controlled trial.

Meeting people's needs is positively correlated with their recovery. However, recovery services rarely include nurse-led programs tailored to the needs of these people. This study aimed to evaluate the effectiveness of a new needs-tailored recovery program by using a cluster-randomized controlled trial design. We conducted a parallel randomized controlled trial in two community psychiatric departments, employing nurse-level clustering for intervention delivery and selecting participants through convenience sampling. The participants were people diagnosed with schizophrenia that were receiving homecare services. The experimental group (n = 82) received needs-tailored recovery program for six months. The control group (n = 82) received traditional homecare. Data were collected at baseline, post-intervention, and the three-month follow-up (the study ran from February to December 2021). The outcomes were recovery, needs, hope, empowerment, psychotic symptoms, and medication adherence. We used repeated measures ANOVA tests to examine the effect of the group × time interaction. The participants in the experimental group demonstrated statistically significant improvements in recovery, hope, and medication adherence compared to the control group, both immediately post-intervention and at the three-month follow-up. Moreover, they exhibited statistically significant reductions in needs compared to the control group at the three-month follow-up (p < .05). While the interaction effect for psychotic symptoms was not significant, the time effect was significant (p < .05). No significant interaction or time effect was observed for empowerment. The findings increase our understanding of recovery-oriented care that prioritizes therapeutic alliance, integrated needs assessment, individual goals, hope, and empowerment. The Clinicaltrials.gov identifier NCT05304780 retrospectively registered on 03/31/2022.

Coupling of surface plasmon resonance and mass spectrometry for molecular interaction studies in drug discovery.

Various analytical technologies have been developed for the study of target-ligand interactions. The combination of these technologies gives pivotal information on the binding mechanism, kinetics, affinity, residence time, and changes in molecular structures. Mass spectrometry (MS) offers structural information, enabling the identification and quantification of target-ligand interactions. Surface plasmon resonance (SPR) provides kinetic information on target-ligand interaction in real time. The coupling of MS and SPR complements each other in the studies of target-ligand interactions. Over the last two decades, the capabilities and added values of SPR-MS have been reported. This review summarizes and highlights the benefits, applications, and potential for further research of the SPR-MS approach.

Longitudinal comparisons in health, participation, life satisfaction, and depressive symptoms from pre-to post Covid 19 pandemic among people with spinal cord injuries.

To identify changes in health, healthcare utilization, participation, and life satisfaction and depressive symptoms from pre-pandemic to post-pandemic among ambulatory and nonambulatory participants with spinal cord injury (SCI). Longitudinal study with the first measurement taken within three months prior to pandemic restrictions and two follow-ups at approximately one year intervals. Medical University in the Southeastern USA. 219 adult participants with SCI, including ambulatory (n= 155) and nonambulatory (n= 64). Self-report assessment of health outcomes from the Behavioral Risk Factor Surveillance System; health service utilization including physician visits, emergency department visits and hospitalizations; items from the Craig Handicap Assessment Reporting Technique; three life satisfaction scales from the Life Situation Questionnaire, and the brief version of the Patient Health Questionnaire. Not applicable. After using a Z score correction for nonnormality, none of the time effects or interaction effects of time by ambulatory status were significant. Six comparisons between ambulatory and nonambulatory were statistically significant. Ambulatory participants reported significantly 3 more days in poor physical health (p = .02) and 2 more days feeling worried, tense, or anxious in the last 30 days (p = .03). They visited emergency department on 0.3 fewer occasions (p = .02), while reporting leaving the house 1 more day every week (p = .02), 2 hours more sitting tolerance (p<.01), and 1 higher score of vocational satisfaction (p = .03). The absence of statistically significant changes from pre- to post-pandemic and the absence of time by ambulatory status interactions suggest stability of outcomes, even in the presence of pandemic challenges.

Effects of 3-nitrooxypropanol (3-NOP, Bovaer®10) and whole cottonseed on milk production and enteric methane emissions from dairy cows under Swiss management conditions.

The objective of this study was to determine the potential effect and interaction of 3- nitrooxypropanol (3-NOP; Bovaer®) and whole cottonseed (WCS) on lactational performance, and enteric methane (CH4) emission of dairy cows. A total of 16 multiparous cows, including 8 Holstein Friesian (HF) and 8 Brown Swiss (BS) [224 ± 36 d in milk, 26 ± 3.7 kg milk yield], were used in a split-plot design, where the main plot was the breed of cows. Within each subplot, cows were randomly assigned to a treatment sequence in a replicated 4 × 4 Latin Square design with 2 × 2 factorial arrangements of treatments with 4, 24-d periods. The experimental treatments were: 1) Control (basal TMR), 2) 3-NOP (60 mg/kg TMR DM), 3) WCS (5% TMR DM), and 4) 3-NOP + WCS. The treatment diets were balanced for ether extract, crude protein, and NDF contents (4%, 16%, and 43% of TMR DM, respectively). The basal diets were fed twice daily at 0800 and 1800 h. Dry matter intake (DMI) and milk yield were measured daily, and enteric gas emissions were measured (using the GreenFeed system) during the last 3 d of each 24-d experimental period when animals were housed in tie stalls. There was no difference in DMI on treatment level, whereas the WCS treatment increased ECM yield and milk fat yield. There was no interaction of 3-NOP and WCS for any of the enteric gas emission parameters, but 3-NOP decreased CH4 production (g/d), CH4 yield (g/kg DMI), and CH4 intensity (g/kg ECM) by 13, 14 and 13%, respectively. Further, an unexpected interaction of breed by 3-NOP was observed for different enteric CH4 emission metrics: HF cows had a greater CH4 mitigation effect compared with BS cows for CH4 production (g/d; 18 vs. 8%), CH4 intensity (g/kg MY; 19% vs. 3%) and CH4 intensity (g/kg ECM; 19 vs. 4%). Hydrogen production was increased by 2.85 folds in HF and 1.53 folds in BS cows receiving 3-NOP. Further, there was a 3-NOP ' Time interaction for both breeds. In BS cows, 3-NOP tended to reduce CH4 production by 18% at around 4 h after morning feeding but no effect was observed at other time points. In HF cows, the greatest mitigation effect of 3-NOP (29.6%) was observed immediately after morning feeding and it persisted at around 23% to 26% for 10 h until the second feed provision, and 3 h thereafter, in the evening. In conclusion, supplementing 3-NOP at 60 mg/kg DM to a high fiber diet resulted in 18 to 19% reduction in enteric CH4 emission in Swiss Holstein Friesian cows. The lower response to 3-NOP by BS cows was unexpected and has not been observed in other studies. These results should be interpreted with caution due to low number of cows per breed. Lastly, supplementing WCS at 5% of DM improved ECM and milk fat yield but did not enhance CH4 inhibition effect of 3-NOP of dairy cows.

Patients' Preferences for Sphingosine-1-Phosphate Receptor Modulators in Multiple Sclerosis Based on Clinical Management Considerations: A Choice Experiment.

Several sphingosine-1-phosphate receptor (S1PR) modulators are available in the US for treating relapsing forms of multiple sclerosis (RMS). Given that these S1PR modulators have similar efficacy and safety, patients may consider the clinical management characteristics of the S1PR modulators when deciding among treatments. However, none of the S1PR modulators is clearly superior in every aspect of clinical management, and for some treatments, clinical management varies based on a patient's comorbid health conditions (e.g., heart conditions [HC]). This study aimed to determine which S1PR modulator patients with relapsing-remitting multiple sclerosis (RRMS) would prefer based on clinical management considerations, and to estimate how different clinical management considerations might drive these preferences. Preferences were explored separately for patients with and without comorbid HC. A multicriteria decision analysis was conducted on S1PR modulators approved to treat RMS: fingolimod, ozanimod, siponimod, and ponesimod. Clinical management preferences of patients with RRMS were elicited in a discrete choice experiment (DCE) in which participants repeatedly chose between hypothetical S1PR modulator profiles based on their clinical management attributes. Attributes included first-dose observations, genotyping, liver function tests, eye examinations, drug-drug interactions, interactions with antidepressants, interactions with foods high in tyramine, and immune system recovery time. Preferences were estimated separately for patients with HC and without HC (noHC). Marginal utilities were calculated from the DCE data for each attribute and level using a mixed logit model. In the multicriteria decision analysis, partial value scores were created by applying the marginal utilities for each attribute and level to the real-world profiles of S1PR modulators. Partial value scores were summed to determine an overall clinical management value score for each S1PR modulator. Four hundred patients with RRMS completed the DCE. Ponesimod had the highest overall value score for patients both without (n=341) and with (n=59) HC (noHC: 5.1; HC: 4.0), followed by siponimod (noHC: 4.9; HC: 3.3), fingolimod (noHC: 3.4; HC: 2.8), and ozanimod (noHC: 0.9; HC: 0.8). Overall, immune system recovery time contributed the highest partial value scores (noHC: up to 1.9 points; HC: up to 1.2 points), followed by the number of drug-drug interactions (noHC: up to 1.2 points; HC: up to 1.7 points). When considering the clinical management of S1PR modulators, the average patient with RRMS is expected to choose a treatment with shorter immune system recovery time and fewer interactions with other drugs. Patients both with and without heart conditions are likely to prefer the clinical management profile of ponesimod over those of siponimod, fingolimod, and ozanimod. This information can help inform recommendations for treating RRMS and facilitate shared decision making between patients and their doctors.

Directed Assembly of Elastic Fibers via Coacervate Droplet Deposition on Electrospun Templates.

Elastic fibers provide critical elasticity to the arteries, lungs, and other organs. Elastic fiber assembly is a process where soluble tropoelastin is coacervated into liquid droplets, cross-linked, and deposited onto and into microfibrils. While much progress has been made in understanding the biology of this process, questions remain regarding the timing of interactions during assembly. Furthermore, it is unclear to what extent fibrous templates are needed to guide coacervate droplets into the correct architecture. The organization and shaping of coacervate droplets onto a fiber template have never been previously modeled or employed as a strategy for shaping elastin fiber materials. Using an in vitro system consisting of elastin-like polypeptides (ELPs), genipin cross-linker, electrospun polylactic-co-glycolic acid (PLGA) fibers, and tannic acid surface coatings for fibers, we explored ELP coacervation, cross-linking, and deposition onto fiber templates. We demonstrate that integration of coacervate droplets into a fibrous template is primarily influenced by two factors: (1) the balance of coacervation and cross-linking and (2) the surface energy of the fiber templates. The success of this integration affects the mechanical properties of the final fiber network. Our resulting membrane materials exhibit highly tunable morphologies and a range of elastic moduli (0.8-1.6 MPa) comparable to native elastic fibers.

Gravity-induced entanglement between two massive microscopic particles in curved spacetime: II. Friedmann–Lemaître–Robertson–Walker universe

In our previous work (Zhang and Shu in Eur Phys J. C 84(3):256, 2024), we have explored quantum gravity induced entanglement of masses (QGEM) in curved spacetime, observing entanglement formation between particles moving along geodesics in a Schwarzschild spacetime background. We find that long interaction time induces entanglement, even for particles with microscopic mass, addressing decoherence concerns. In this work, we build upon our previous work (Zhang and Shu 2024) by extending our investigation to a time-dependent spacetime. Specifically, we explore the entanglement induced by the mutual gravitation of massive particles in the Friedmann–Lemaître–Robertson–Walker (FLRW) universe. With the help of the phase shift and the QGEM spectrum, our proposed scheme offers a potential method for observing the formation of entanglement caused by the quantum gravity of massive particles as they propagate in the FLRW universe. Consequently, it provides insights into the field of entanglement in cosmology.

Impact of social isolation on corticosterone release and recovery after stroke in aged rats: A behavioral and biochemical analysis

Social isolation (SI) after stroke reduces recovery. The aim of this study was to evaluate the effects of SI on corticosterone release and recovery after stroke in aged rats. A total of 64 male Wistar rats (aged 24 months) were used in the present study. All rats were housed in pairs for two weeks. After two weeks, rats were randomly assigned to one of four groups: (1) rats underwent sham surgery and kept socially isolated (control/social isolated (CO/SI) group); (2) rats underwent sham surgery and kept pair housed (control/pair housed (CO/PH) group); (3) rats underwent middle cerebral artery occlusion (MCAO) surgery and kept socially isolated (stroke/isolated (ST/SI) group); (4) rats underwent MCAO surgery and kept pair housed (stroke/pair housed (ST/PH)) group. Behaviors were assessed using the adhesive removal test, rotarod test and social interaction test at 1st, 7th, 14th and 21st days after stroke. Serum biochemical analysis was also performed on the behavioral testing days. Results showed THAT serum corticosterone and MDA levels in CO/PH group were significantly lower than CO/SI group. Serum BDNF levels in CO/PH group was significantly higher than CO/SI group. Serum corticosterone and MDA levels in ST/PH group were lower than ST/SI group. In ST/PH group, serum Total antioxidant capacity (TAC) and BDNF levels were significantly higher than ST/SI group. Biochemical analysis of certain regions of the brain (hippocampus, striatum and cerebral cortex) was performed on 21st day after stroke. In the hippocampus of CO/PH group, BDNF and TAC levels were significantly higher than CO/SI group. The hippocampal MDA level of CO/PH group were significantly lower than CO/SI group. BDNF and TAC levels in the hippocampus, striatum and cerebral cortex of ST/PH group were significantly higher and MDA level was significantly lower as compared with ST/SI group. Both ischemic groups showed sensorimotor recovery over a 21-day period, but recovery of ST/PH group was significantly greater than ST/SI group. Total social interaction time in ST/PH group was significantly longer than ST/SI group. Based on the results of this study, social interaction after stroke enhances histologic and sensorimotor recovery through reduction of HPA activity and corticosterone release, leading to increased TAC and BDNF levels.

Effects of a ‘Rebuilding Myself’ intervention on enhancing the adaptability of cancer patients to return to work: a randomized controlled trial

ObjectivesTo explore the effects of a ‘Rebuilding Myself’ intervention on enhancing the adaptability of cancer patients to return to work.MethodsA single-center, single-blind, randomized controlled trial design was used. Eligible patients who were receiving routine hospital treatment were recruited from the university-affiliated hospital in our city. Patients in the control group only received usual care, while patients in the intervention group received additional ‘Rebuilding Myself’ intervention. Adaptability to return to work, self-efficacy of returning to work, mental resilience, quality of life and work ability were measured at baseline, the 6th and 12th of the intervention. The general estimation equations were used to compare the overall changes of each outcome index between the two groups at different time points. Considering that there may be patient shedding and rejection, Per-Protocol and Intention-to-Treat analysis were used to analyze the data in this study.ResultsThere were statistically significant differences between the two groups of patients in the cancer patients’ adaptability to return to work, self-efficacy to return to work, mental resilience, work abilities, the physical, emotional, cognitive function, fatigue, insomnia and overall health status dimensions of quality of life (P < 0.05). And no significant difference was found in other dimensions (P > 0.05). The group effect, time effect, and interaction effect of patients’ return to work adaptability and return to work self-efficacy were statistically significant in both groups (P < 0.05). Mental resilience, working ability, and quality of life had obvious time effect and interaction effect (P < 0.05).ConclusionThis intervention could improve cancer patients’ adaptability to return to work, self-efficacy to return to work, mental resilience, work abilities and quality of life. And it can be further expanded to improve the adaptability of patients to return to work, then to help patients achieve comprehensive rehabilitation.Implications for cancer survivorsThe application of ‘Rebuilding Myself’ interventions can effectively improve the adaptability of cancer patients returning to work.Trial registrationThis study was registered at the Chinese Clinical Trial Registry (Registration number: ChiCTR2200057943) on 23 March, 2022.

Influence of acute dietary nitrate supplementation on oxygen delivery/consumption and critical impulse during maximal effort forearm exercise in males: a randomized crossover trial.

Beetroot juice supplementation (BRJ) increases nitric oxide bioavailability under conditions of hypoxia and acidosis, characteristics of maximal effort exercise, which is required to identify forearm critical impulse. We hypothesized BRJ would improve oxygen delivery:demand matching and forearm critical impulse performance. Healthy males (20.8±2.4 years) participated in a randomized crossover trial between October 2017-May 2018 (Queen's University, Kingston, ON). Participants completed 10-minutes of rhythmic maximal effort forearm handgrip exercise 2.5 hours post placebo (PL) vs. BRJ (9 completed PL/BRJ vs. 4 completed BRJ/PL) within a 2 week period. Data are presented as mean±SD. There was a main effect of drink (PL > BRJ) for oxygen extraction (P=0.033,ηp2=0.351) and oxygen consumption/force (P=0.017,ηp2=0.417). There was a drink x time interaction (PL > BRJ) for oxygen consumption/force (P=0.035,ηp2=0.216) between 75-360s (1.25-6 min) from exercise onset. BRJ did not influence oxygen delivery (P=0.953,ηp2=0.000), oxygen consumption (P=0.064,ηp2=0.278), metabolites [[lactate] (P=0.196,ηp2=0.135), pH (P=0.759,ηp2=0.008)] or power-duration performance parameters [critical impulse (P=0.379,d=0.253), W' (P=0.733,d=0.097)]. BRJ during all-out handgrip exercise does not influence oxygen delivery or exercise performance. Oxygen cost of contraction with BRJ is reduced as contraction impulse is declining during maximal effort exercise resulting in less oxygen extraction. https://osf.io/pga37/registrations.

Yoga improves self-reported cognitive function among cancer survivors: results from the STAYFit trial

IntroductionVarious physical activity-based interventions have been tested to determine their efficacy in improving cancer related cognitive decline (CRCD), however the role of mind-body practices such as yoga remains to be explored. In this manuscript we present preliminary effects of yoga vs. aerobic and stretching-toning modalities of exercise on CRCD among adult cancer survivors.MethodsParticipants (N = 78) were randomized to one of the three exercise groups for a duration of 12-weeks and engaged in ≥150 min per week of supervised group exercises. At baseline and following the 12-week interventions, participants completed the Functional Assessment of Cancer Therapy–Cognitive Function.ResultsResults demonstrated a significant group*time interaction for FACT-Cog perceived cognitive abilities subscale, with participants in the yoga group demonstrating a significant increase as compared to the aerobic and stretching-toning groups. The FACT-Cog total score showed a significant time effect with all groups demonstrating a significant increase at follow-up. Other subscales did not show any significant improvements.DiscussionThese findings provide promising evidence for the effects of yoga on self-reported cognitive function in cancer survivors. Notably, 12-weeks of yoga showed an increase in the perceived cognitive abilities and demonstrated a clinically meaningful increase in total cognitive function as measured by the FACT-Cog, suggesting that this exercise modality has the potential to impact this important health outcome during cancer survivorship.Trial registrationClinicalTrials.gov, identifier: NCT03650322.

Investigation of Two Laser Heat Treatment Strategies for Local Softening of a Sheet in Age-Hardening Aluminum Alloy by Means of Physical Simulation

AbstractCar manufacturers increasingly aid high-strength aluminum alloys for their advantageous weight-to-strength ratio, but their limited formability poses challenges in plastic deformation processes. Tailored heat-treated blanks (THTBs) are a propitious approach to improve formability. Surface laser treatment is the predominant technology for obtaining THTB. To design this process quickly and accurately, without material waste, the use of physical simulation is increasingly promising. It allows replicating the process on a lab-scale and studying posttreatment mechanical and metallurgical properties. By adopting Gleeble® physical simulator, this study investigates the softening effects of local surface laser heat treatment on a EN AW 6082 T6 aluminum alloy blank. Two laser movement strategies—single linear path and multiple rectangular paths—were investigated at two treatment speeds for each. A finite element (FE) model was developed for simulating the process under all explored conditions. FE-derived thermal cycles were reproduced by means of physical simulation. After physical tests, alloy mechanical properties were evaluated. Results show that these properties depend on both the peak temperature thermal cycle and the interaction time between the laser source and the material surface. The comparison between the two strategies revealed that the multiple rectangular paths strategy allows to achieve a wider softened area at comparable interaction times.

Using generative AI in human resource development: an applied research study

ABSTRACT In this article, we report results of an experimental study investigating the use of ChatGPT in the design of an HRD intervention. Our goal was to identify the benefits and drawbacks of using this technology in HRD practice. The theoretical framework for the study was provided by the Activity Theory, an often used approach in Human-Computer Interaction research. The study participants were HRD doctoral students from two large public universities in the USA. The participants used ChatGPT in developing an HRD intervention plan, based on a supplied organisational scenario. Data for the analysis included the intervention plans, prompts used by participants, outputs received from ChatGPT, and participants’ responses to open-ended questions. The study revealed varied participant experiences with ChatGPT, including differences in time investment and interaction rules. Among key findings were the tendency to interact with ChatGPT as if it were a human consultant, and steps to establish division of labour between the tool and the human agent. ChatGPT was valued for its usefulness in initial planning and brainstorming ideas for HRD interventions, yet its limitations were apparent in generic responses and lack of contextual understanding. Across all participants, the crucial role of human expertise in refining ChatGPT’s output was consistently highlighted.

Original Investigation: Manipulating energy availability in male endurance runners: a randomised controlled trial.

This study investigated the effect of four days low energy availability (LEA) on physiological markers and mood states in male endurance runners. Twelve participants (mean (standard deviation)); age: 25.8 (3.8) years; fat free mass (FFM): 52.8 (5.5) kg) completed three 4-day conditions: Adequate Energy Availability, AEA: 45 kcal/kgFFM/day; LEA1: 30 kcal/kgFFM/day; and LEA2: 15 kcal/kgFFM/day), in a randomized order. Participants ran on a treadmill at 65% of V̇O2max until they expended 15 kcal/kg FFM/day of energy. Energy intake was adjusted to achieve the desired EA. Pre- and post- measurements of bone turnover, metabolism, testosterone and estradiol (plasma), resting metabolic rate (indirect calorimetry), and mood states (Brunel Mood Scale) were assessed. The results reported a significant decrease in testosterone (condition × time interaction, p=0.03) occurred on LEA2 (Pre: 23.8 (7.0) nmol/L vs Post: 20.3 (7.7) nmol/L) compared with AEA (Pre: 22.9 (5.5) nmol/L vs Post: 23.3 (6.1) nmol/L) or LEA1 (Pre: 23.6 (8.6) nmol/L vs Post: 20.9 (8.8) nmol/L). Fatigue level significantly increased (condition × time interaction, p=0.02) in LEA2 (Pre: 3.5 (1.7) vs Post: 6.5 (2.9)) but did not change in AEA (Pre: 2.8 (1.5) vs Post: 2.5 (2.7)) or LEA1 (Pre: 2.8(2.4) vs Post: 2.9 (2.0)). Other measures were unaffected by the interventions. In conclusion, this study suggests testosterone and fatigue may serve as early indicators of LEA in male runners. However, other physiological markers and mood states appeared largely unaffected, aligning with existing literature indicating minimal disruption of physiological functions during acute LEA in male athletes.

Translational Research of the Acute Effects of Negative Emotions on Vascular Endothelial Health: Findings From a Randomized Controlled Study.

Provoked anger is associated with an increased risk of cardiovascular disease events. The underlying mechanism linking provoked anger as well as other core negative emotions including anxiety and sadness to cardiovascular disease remain unknown. The study objective was to examine the acute effects of provoked anger, and secondarily, anxiety and sadness on endothelial cell health. Apparently healthy adult participants (n=280) were randomized to an 8-minute anger recall task, a depressed mood recall task, an anxiety recall task, or an emotionally neutral condition. Pre-/post-assessments of endothelial health including endothelium-dependent vasodilation (reactive hyperemia index), circulating endothelial cell-derived microparticles (CD62E+, CD31+/CD42-, and CD31+/Annexin V+) and circulating bone marrow-derived endothelial progenitor cells (CD34+/CD133+/kinase insert domain receptor+ endothelial progenitor cells and CD34+/kinase insert domain receptor+ endothelial progenitor cells) were measured. There was a group×time interaction for the anger versus neutral condition on the change in reactive hyperemia index score from baseline to 40 minutes (P=0.007) with a mean±SD change in reactive hyperemia index score of 0.20±0.67 and 0.50±0.60 in the anger and neutral conditions, respectively. For the change in reactive hyperemia index score, the anxiety versus neutral condition group by time interaction approached but did not reach statistical significance (P=0.054), and the sadness versus neutral condition group by time interaction was not statistically significant (P=0.160). There were no consistent statistically significant group×time interactions for the anger, anxiety, and sadness versus neutral condition on endothelial cell-derived microparticles and endothelial progenitor cells from baseline to 40 minutes. In this randomized controlled experimental study, a brief provocation of anger adversely affected endothelial cell health by impairing endothelium-dependent vasodilation.

Stimulant medication use and apparent cortical thickness development in attention-deficit/hyperactivity disorder: a prospective longitudinal study

BackgroundStimulant medication is commonly prescribed as treatment for attention-deficit/hyperactivity disorder (ADHD). While we previously found that short-term stimulant-treatment influences apparent cortical thickness development in an age-dependent manner, it remains unknown whether these effects persist throughout development into adulthood.PurposeInvestigate the long-term age-dependent effects of stimulant medication use on apparent cortical thickness development in adolescents and adults previously diagnosed with ADHD.MethodsThis prospective study included the baseline and 4-year follow-up assessment of the “effects of Psychotropic drugs On the Developing brain-MPH” (“ePOD-MPH”) project, conducted between June-1-2011 and December-28-2019. The analyses were pre-registered (https://doi.org/10.17605/OSF.IO/32BHF). T1-weighted MR scans were obtained from male adolescents and adults, and cortical thickness was estimated for predefined regions of interest (ROIs) using Freesurfer. We determined medication use and assessed symptoms of ADHD, anxiety, and depression at both time points. Linear mixed models were constructed to assess main effects and interactions of stimulant medication use, time, and age group on regional apparent cortical thickness.ResultsA total of 32 male adolescents (aged mean ± SD, 11.2 ± 0.9 years at baseline) and 24 men (aged mean ± SD, 29.9 ± 5.0 years at baseline) were included that previously participated in the ePOD-MPH project. We found no evidence for long-term effects of stimulant medication use on ROI apparent cortical thickness. As expected, we did find age-by-time interaction effects in all ROIs (left prefrontal ROI: P=.002, right medial and posterior ROIs: P&amp;lt;.001), reflecting reductions in apparent cortical thickness in adolescents. Additionally, ADHD symptom severity (adolescents: P&amp;lt;.001, adults: P=.001) and anxiety symptoms (adolescents: P=0.03) were reduced, and more improvement of ADHD symptoms was associated with higher medication use in adults (P=0.001).ConclusionWe found no evidence for long-term effects of stimulant-treatment for ADHD on apparent cortical thickness development in adolescents and adults. The identified age-dependent differences in apparent cortical thickness development are consistent with existing literature on typical cortical development.

The Edifice of Vasculature-On-Chips: A Focused Review on the Key Elements and Assembly of Angiogenesis Models.

The conception of vascularized organ-on-a-chip models provides researchers with the ability to supply controlled biological and physical cues that simulate the in vivo dynamic microphysiological environment of native blood vessels. The intention of this niche research area is to improve our understanding of the role of the vasculature in health or disease progression in vitro by allowing researchers to monitor angiogenic responses and cell-cell or cell-matrix interactions in real time. This review offers a comprehensive overview of the essential elements, including cells, biomaterials, microenvironmental factors, microfluidic chip design, and standard validation procedures that currently govern angiogenesis-on-a-chip assemblies. In addition, we emphasize the importance of incorporating a microvasculature component into organ-on-chip devices in critical biomedical research areas, such as tissue engineering, drug discovery, and disease modeling. Ultimately, advances in this area of research could provide innovative solutions and a personalized approach to ongoing medical challenges.

298 Impact of ruminal acidosis on cattle energy metabolism

Abstract The objective of this study was to determine the impact of a bout of ruminal acidosis on energy metabolism of cattle unadapted to a high concentrate diet. Eleven ruminally cannulated steers [body weight (BW) = 352 kg ± 27] were blocked into 3 groups based on initial BW. Before the start of the experiment, animals were consuming a forage-based diet as well as adapted to the headbox style respiration chambers. Additionally, before the experiment, gas emission data were collected over a 24-h period when cattle received an ad libitum forage-based diet for use as a covariate in the statistical analysis. For the experiment, steers were moved into headboxes at the conclusion of a 24-h fast and subsequently received 1 of 2 treatment diets: control (CON), forage-based diet or acidosis (AC), concentrate-based diet. Steers remained in the headboxes for 48 h. Gas concentrations from each headbox were collected hourly and analyzed with an infrared photoacoustic gas analyzer. Ruminal pH, fecal pH, volatile fatty acids, and lactate were also measured during the acidosis challenge. Data were analyzed with the MIXED procedure of SAS 9.4. A treatment × day effect (P = 0.03) was observed for dry matter intake with intake being similar for both CON and AC steers on d 1 but AC steers consuming 2.02 kg less on d 2. Treatment affected ruminal pH (P &amp;lt; 0.01) as CON steers had a greater ruminal pH than AC steers. Greater total volatile fatty acids (P &amp;lt; 0.01) were observed for steers on AC treatment compared with CON. A treatment × time interaction (P &amp;lt; 0.01) was observed for ruminal lactate concentration with AC steers having greater concentrations from h 16 to 36. Fecal pH was affected by a treatment × time interaction (P = 0.05) as AC steers had lower fecal pH from h 24 to h 32. Over the 48-h observation period, there was a treatment × time interaction (P = 0.04) for carbon dioxide production as AC steers produced less CO2 after h 24. No treatment × time interaction was detected for oxygen consumption. However, there was a time effect (P &amp;lt; 0.01). Respiratory quotient was not affected by treatment, day, or their interaction (P ≥ 0.19). Heat production tended (P = 0.06) to be different between treatments, with AC having less heat production than CON. Thus, the effects of ruminal acidosis on gas emissions and energetics may be more transient during the time course of an acidotic bout or have a longer duration even after ruminal pH has recovered.