A transfer radiometer (TR) applied to an on-orbit radiometric benchmark transfer chain has been developed, which can achieve the high-precision transformation of power and radiance responsivity and transmit the radiance responsivity traced to the cryogenic radiometer to remote sensors, such as an imaging spectrometer, so that the on-orbit remote sensors can achieve the high accuracy calibration of 10−3 magnitude. Radiance comparison experiments between the TR and the radiance standard of the National Institute of Metrology (NIM) were carried out to demonstrate the absolute accuracy of the TR radiance measurement. At 780.0 nm and 851.9 nm, the relative measurement uncertainties of the TR filter-free channel were 0.24% (k = 1). Additionally, the radiance measurement results of the TR were consistent with those of the NIM radiance meter, and the radiance measurement results’ relative differences between the TR and the NIM radiance meter were approximately 0.04% at 780.0 nm and 851.9 nm. The relative measurement uncertainties of TR 780.4 nm and 851.8 nm filter channels were 0.89% (k = 1) and 0.84% (k = 1), respectively. Additionally, the radiance measurement results of the TR 780.4 nm and 851.8 nm filter channels were consistent with the radiances of the integrating sphere source calibrated by the NIM at 780.4 nm and 851.8 nm; the relative differences between the radiances measured by the two TR filter channels and the radiances of the integrating sphere source itself were better than 0.56%. This proved that the TR could measure the monochromatic source radiance with a measurement uncertainty of 0.24% and measure the broadband source radiance with a measurement uncertainty better than 0.89%. The TR can be applied to the radiometric benchmark transfer chain to improve the measurement precision of on-orbit remote-sensing instruments.