Insulin Resistance In Postmenopausal Women Research Articles

Whole-Body Cryotherapy Improves Asprosin Secretion and Insulin Sensitivity in Postmenopausal Women-Perspectives in the Management of Type 2 Diabetes.

Type 2 diabetes (T2DM) is a global problem. The effect of whole-body cryotherapy (WBC) on metabolism in humans is postulated. The aim of this study was to determine the effect of WBC on asprosin concentrations, glucose homeostasis and insulin resistance in postmenopausal women with T2DM. Changes in fasting blood glucose (FBG), glycated haemoglobin (HbA1c), insulin, asprosin, insulin-resistance indices (HOMA-IR, Quicki), the triglyceride-glucose index (TyG) and C-reactive protein (CRP) were determined. Determination was carried out after 30 WBCs (3 min, -120 °C), applied in six series of five treatments, with 2-day breaks in postmenopausal women with T2DM and the results were compared to changes in postmenopausal women without T2DM (CON). Blood was collected before 1 WBC (T0), after 30 WBCs (T1) and 2 weeks after their completion (T2). In the T2DM group, there was a significant decrease in FBG and HbA1c in T1 and T2, as well as a significant decrease in insulin, HOMA-IR and CRP, and an increase in the Quicki index in T2. In the CON group, the concentration of asprosin at T2 was significantly lower than at T0. There was a significantly positive correlation between asprosin and FBG and HOMA-IR, and a trend towards a decrease of asprosin concentration in T2 in postmenopausal women with T2DM.

Relationship between vitamin B12 levels and insulin resistance in postmenopausal women from Colombia Caribbean

Introduction: The high prevalence of low vitamin B12 serum levels has been recognized as a public health problem in Latin America; however, the current magnitude of this deficiency in Colombia is uncertain. Low levels of vitamin B12 can induce clinical and subclinical hematological and neurological disorders. Epidemiological studies have demonstrated a relationship between vitamin B12 deficiency and cardiovascular diseases (CVDs). However, the role of vitamin B12 in insulin resistance has been poorly studied. Objective: This study aimed to evaluate the relationship between vitamin B12 serum levels and biochemical and anthropometric markers related to CVDs and insulin resistance in postmenopausal women from Colombia Caribbean. Methods: Correlational, descriptive study. By convenience sampling, 182 postmenopausal women from the medical consultation service of a health institution were linked. Serum vitamin B12 levels, anthropometric variables (body mass index, abdominal perimeter), and biochemical variables (glycemia, insulin, lipid profile, HOMA IR) were evaluated. Results: The average value of the vitamin B12 serum level was 312.5 ± 122.5 pg/mL (230.6 ± 90.4 pmol/L); 46.7% of the women had less than adequate levels of 300 pg/mL (> 221 pmol/L), and 9. 9% were deficient, with levels of less than 200 pg/mL (148 pmol/L). The women with metabolic syndrome were 63.7%, and according to HOMA IR, 52.7 % had insulin resistance. A significant inverse relationship was shown between serum vitamin B12 levels with basal glycemic (P =0.002) and HOMA-IR (P =0.040). Conclusions: A significant inverse relationship between vitamin B12 levels and basal glycemia and HOMA-IR was observed. These findings highlight vitamin B12 deficiency in postmenopausal women and suggest nutritional supplementation.

Abstract 3030: Effect of 6 months of bazedoxifene and conjugated estrogen on measures of insulin resistance in postmenopausal women at increased risk for breast cancer

Abstract Background: Bazedoxifene 20 mg (BZA) + Conjugated estrogen 0.45 mg (CE) as Duavee™ is FDA approved for treatment of hot-flashes and prevention of osteoporosis. We are investigating 6 months of BZA + CE vs wait list control in a multisite Phase IIB trial for primary prevention of breast cancer in high-risk peri-and postmenopausal women with vasomotor symptoms. Primary endpoints are change in mammographic fibroglandular volume and normal breast epithelial proliferation as assessed by Ki-67. Menopause transition is marked by an increase in insulin resistance and incidence of metabolic syndrome which in turn are implicated in the development of breast cancer. Tamoxifen can induce insulin resistance due to effects on small adipocytes, pancreatic beta cells, and hepatic lipid accumulation. In contrast, preclinical studies suggest that BZA + CE may have favorable effects on insulin resistance, particularly in obese animals. Consequently, we are exploring effects of BZA + CE on measures of insulin resistance in our clinical trial. Methods: Fasting plasma was assessed for insulin and glucose in a CLIA laboratory the same day as drawn. HOMA-IR (insulin resistance) was computed as insulin (µU/ml) × glucose (mg/dl)]/405) and HOMA-%S (measure of insulin sensitivity) as 100/HOMA-IR. HOMA-IR ≥ 1.9 is considered an indication of early insulin resistance and HOMA-IR >2.8 significant insulin resistance. HOMA-%S values of ≥100% are considered an indication of insulin sensitivity. Results: 21 women have been randomized in the initial 10 months of accrual at the University of Kansas Cancer Center with 8 women completing the 6 -month randomized period to date. Median age was 53 (48-56), and median BMI was 25 kg/m2 (22-35 kg/m2). All women were normoglycemic. However, 2 women (1 each BZA + CE and wait list) had baseline evidence of insulin resistance by HOMA IR (≥1.9). In the woman randomized to BZA + CE, HOMA-IR improved from 2.29 at baseline to 1.0 at 6 months and HOMA-%S improved from 44 to 100%. There was little change in insulin resistance measures in the woman with baseline insulin resistance randomized to wait list. HOMA-IR was 2.83 at baseline and 2.76 at 6 months and HOMA-%S 35% at baseline and 36% at 6 months. Another waitlist participant with normal baseline HOMA-%S of 141% dropped to 71% at 6 months. Results will be available for 20 study participants at the meeting. Summary: In our initial accrual group there is no indication that 6 months of BZA +CE is associated with development of insulin resistance. Funded in part by NIH grant CA249437 and Breast Cancer Research Foundation grants BCRF-21-049 and BCRF-22-049. Citation Format: Carol J. Fabian, Erin D. Giles, Katherine L. Cook, Kandy Powers, Christy Altman, Amy Kreutzjans, Teresa Phillips, Adrian Zelenchuk, Krystal Pittman, Stephen D. Hursting, Bruce Kimler. Effect of 6 months of bazedoxifene and conjugated estrogen on measures of insulin resistance in postmenopausal women at increased risk for breast cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 3030.

Concurrent exercise and aerobic-resistance exercise ameliorate the serum of Retinol-Binding Protein-4 level and insulin resistance in postmenopausal women

The plasma concentration of retinol-binding protein-4 is higher in older women than younger women. This study determined the effect of concurrent exercise on the serum concentration of retinol-binding protein-4 and postmenopausal women's insulin resistance. Based on an In-silico study and bioinformatic analysis, 31 genes are overlapped between Cardiovascular diseases, Diabetes, Hypertension, and Obesity. Enrichment and data mining performed in STRING, KEGG, Reactome, DAVID, and BIOGRID. Finally, interactions between hub genes, pathways, and disease designed with Cytoscape software. Moreover, Twenty healthy, physically inactive postmenopausal women with an average age of 65.45 ± 2.52 years old and weight of 82.83 ± 11.98 Kg were selected in a targeted manner to participate and were divided into two Treated ( n = 10) and control ( n = 10) groups randomly. Physical composition, serum glucose level, serum insulin, and retinol-binding protein-4 were measured in both groups before and after the intervention. The experimental group participated in 12 weeks of combinatory exercise. Data mining illustrated RBP4 involved in lipid metabolisms, inflammation pathway, Cytokine Signaling in Immune system, Retinoid metabolism disease, Retinoid Metabolism, and Transport. We found that Combinatory exercises significantly reduced plasma retinol-binding protein-4, glucose, insulin, insulin resistance. We analyzed data collected from the bioinformatics database and indicated that RBP4 increased via up-regulation of lipogenesis and overexpressed adipokines in obesity and inactivity conditions. We predicted that physical activity via increasing β-oxidation of free fatty acid and lipolysis affects RBP4, glucose, and insulin production modulation. Combinatory exercises caused a reduction in body composition and resistance to insulin and reduced the retinol-binding protein-4 level. La concentration plasmatique de la protéine de liaison du rétinol (RBP4 pour retinol binding protein 4 ) est plus élevée chez les femmes avançant en âge, comparativement aux femmes plus jeunes. Cette étude a déterminé l’effet de l’entrainement combiné de la force et de l’endurance sur la concentration sérique de RBP4 et la résistance à l’insuline des femmes ménopausées. Sur la base d’une étude in-silico et d’une analyse bioinformatique, on retrouve 31 gènes communs entre les maladies cardiovasculaires, le diabète, l’hypertension artérielle et l’obésité. Une analyse en data mining (procédé permettant de retrouver des corrélations et d’extraire des connaissances de plusieurs bases de données) a été réalisée sur les bases de données String, Kegg, Reactome, David et Biogrid. Enfin, les interactions potentielles entre les gènes en réseau (hub genes), les voies de signalisation et les maladies cibles ont été déterminées avec le logiciel Cytoscape. De plus, vingt femmes ménopausées en bonne santé et physiquement inactives, âgées en moyenne de 65,45 ± 2,52 ans et pesant 82,83 ± 11,98 kg ont été sélectionnées pour participer à l’étude clinique et ont été divisées de manière aléatoire en deux groupes, entrainées ( n = 10) et témoins ( n = 10). La composition corporelle, le taux de glucose sanguin, l’insuline et la RBP4 ont été mesurés dans les deux groupes avant et après le programme d’entrainement. Le groupe de femmes entrainées a participé à 12 semaines d’exercices combinés. L’exploration de données en data mining a montré que la RBP4 est impliquée dans le métabolisme des lipides, l’inflammation, la signalisation des cytokines dans le système immunitaire, les maladies du métabolisme du rétinol, le métabolisme des rétinoïdes et leur transport. Nous montrons que les exercices combinés de la force et de l’endurance réduisent de manière significative la RBP4 plasmatique, le glucose, l’insuline et la résistance à l’insuline. Nous avons analysé les données collectées à partir de bases de données bioinformatiques et montré que la RBP4 augmentait via une régulation positive de la lipogenèse et des adipokines surexprimées dans des conditions d’obésité et d’inactivité. L’activité physique via l’augmentation de la β-oxydation des acides gras libres et la lipolyse affecte la modulation de la production de RBP4, de glucose et d’insuline. Un programme de 12 semaines d’exercices combinés a entraîné une correction de la composition corporelle, une réduction de la résistance à l’insuline et une réduction du taux de RBP4.

Interleukin-15 are associated with insulin resistance in postmenopausal women with metabolic syndrome

Objective To determine if higher levels of circulating interleukin (IL)-15 are positively associated with improvement in insulin resistance in postmenopausal women (PW) with metabolic syndrome (MS). Methods According to the median value of IL-15 at baseline, PW older than or equal to 45 years were divided into two groups: higher (n = 43) and lower (n = 42) IL-15. There was a 9-month follow-up period with clinical assessments at baseline and at 9 months (criteria of metabolic syndrome, body fat, and insulin resistance). Insulin resistance (IR) was calculated according to the Homeostasis Model Assessment-estimated insulin resistance (HOMA-IR). For IL-1β, IL-6, IL-10, IL-13, IL-33, IL-15, and TNF-α was determined using immunoassay Magnetic Bead Panel. Results There was an interaction between the time and group only for insulin (p = .008) and HOMA-IR (p = .024). After adjusting for confounding variables (clinical and ILs), the HOMA-IR (p = .006) and insulin (p = .003) were lower in the higher-IL-15 group [HOMA-IR: 2.2 (95% CI: 1.9–2.5) and insulin: 9.1 µIU/mL (95% CI: 7.9–10.3)] when compared to the lower-IL-15 group [HOMA-IR: 3.1 (95% CI: 2.6–3.6) and insulin: 12.9 (95% CI: 11.1–14.9)] after 9 months of follow-up. Conclusion Higher levels of circulating IL-15 are positively associated with improvements in IR in PW with MS.

Heterogeneity in resistance training-induced muscle strength responses is associated with training frequency and insulin resistance in postmenopausal women.

In older adults, muscle strength (MS), a key component of sarcopenia, is essential to maintaining independence and physical capacity. The rate of MS decline typically accelerates during the menopausal transition. Although MS has been shown to increase with resistance training (RT), the response to training is quite heterogeneous. Thus, if contributing factors to RT non-responsiveness to MS gains are identified, it may be possible to develop more effective and personalized ways to improve MS or identify individuals who may benefit from RT interventions. This study assessed potential factors that may contribute to MS response heterogeneity in postmenopausal women: training frequency, serum FSH and estrogen levels, adiposity, inflammation marker, and insulin resistance. One hundred and thirteen individuals participated in a 16-week program of supervised RT (3 sets, 8-12 repetitions, and 2-3 times/week). A control group (CTL, n=63 - no performed the RT) was used as the comparator arm. Body composition (skinfold) and blood samples (metabolic and inflammatory indicators and hormones) were measured at baseline. Knee extensor strength (1RM) was measured at baseline, 8weeks, and 16weeks. Only the RT group increased 1RM after 8weeks (RT=14±12% vs. CTL=6±15%). Both groups increased 1RM after 16weeks, with the RT group showing a greater increase than the CTL group (RT=31±23% vs CTL=13±25%). After 8weeks of RT, 41 (36% of total) individuals were considered non-responders (based on control group responses) and 27 (24% of total) individuals after 16weeks. At week 8, lower RT frequency (2 times/week vs. 3 times/week) was associated with higher odds of being non-responder (3 times, P=0.048). At week 16, lower RT frequency (13 times, P=0.009) and higher HOMA-IR (for every unit increase, odds increase by 40%, P=0.022) were associated with higher odds of being non-responder. Higher QUICKI was associated with lower odds of being non-responder (for every unit increase, odds decrease by 16%, P=0.039). Moreover, higher RT frequency (17 times, P=0.028) and higher QUICKI (for every unit increase, odds increase by 41%, P=0.017) were associated with higher odds of becoming a responder at week 16, being a non-responder at week 8. Heterogeneity in RT-induced MS responses is associated with training frequency and insulin resistance in postmenopausal women.

The relationship between insulin resistance, bone mineral density, and fracture risk in postmenopausal women

Background/Aim. Skeletal muscles and bones are essential tissues that, in addition to supporting the body, are the primary site of postprandial glucose intake, which is significantly associated with insulin resistance. The aim of this study was to determine the effect of insulin resistance on bone mineral density (BMD) and fracture risk and re-evaluate the relationship between muscle properties and BMD and insulin resistance in postmenopausal women in Serbia. Methods. Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) was calculated in postmenopausal women who were divided into two groups. The ?cut-off? value of insulin resistance for the group with ?Low HOMA-IR? was < 2, and for ?High HOMA-IR? > 2. Fat mass (FM), lean mass (LM), and BMD were measured on the hip and spine using a densitometer with dual-energy X-ray absorptiometry. Results. FM and LM had an evident impact on BMD. The decrease in LM and fat buildup was associated with a higher incidence of insulin resistance. A positive correlation was confirmed between HOMA-IR and BMD on the spine and hip, but there was no correlation between insulin resistance and fracture risk. Conclusion. LM and FM have significant effects on BMD. The association between LM, FM, BMD and the onset of insulin resistance in postmenopausal women is confirmed. However, women with higher insulin resistance levels and higher BMD do not have a lower fracture risk.

Evaluation of hemorheological parameters as biomarkers of calcium metabolism and insulin resistance in postmenopausal women.

Calcium, vitamin D and insulin resistance are linked to osteoporosis and cardiovascular disease in menopause. Determine if hemorheological parameters related to blood viscosity in microcirculation are linked to calcium metabolism and insulin resistance in menopause. 25-Hydroxyvitamin D (25(OH)D)), 1, 25-dihydroxyvitamin D3 (1, 25(OH)2D), parathyroid hormone, ionized calcium, glucose, insulin and hemoglobin A1c were measured in blood from 43 volunteers. Red blood cells (RBC) aggregation, RBC deformability and whole blood viscosity were also performed. 25(OH)D showed a positive correlation with RBC deformability 0.60 Pa. Subjects with 25(OH)D≤29.00 ng/mL had lower RBC deformability 0.60 Pa, and higher RBC aggregation and higher HOMA-IR. Ionized calcium showed a negative correlation with RBC aggregation. Subjects with ionized calcium ≤1.24 mmol/L showed higher RBC aggregation. There was a positive correlation between HOMA-IR and RBC aggregation and HOMA-IR showed a negative correlation with RBC deformability 0.30 Pa. Subjects with HOMA-IR <1.80 showed lower RBC aggregation and higher RBC deformability at 0.30 Pa, 0.60 Pa, 1.20 Pa, 3.0 Pa and 6.0 Pa. Low 25(OH)D, low ionized calcium and high HOMA-IR are related to impaired hemorheology in menopause. RBC aggregation and deformability can be used as biomarkers of calcium metabolism and insulin resistance in menopause.

Insulin resistance and breast cancer incidence and mortality in postmenopausal women in the Women's Health Initiative.

Insulin resistance is associated with higher all-cause and cancer-specific mortality in postmenopausal women. However, to the authors' knowledge, information regarding insulin resistance and breast cancer mortality risk is limited. Therefore, the authors examined associations between insulin resistance and breast cancer incidence and mortality in a subsample of Women's Health Initiative participants. A total of 22,837 postmenopausal women with fasting baseline glucose and insulin levels were followed for incident breast cancer and breast cancer mortality. Breast cancers were verified by medical record review and serial National Death Index linkage-enhanced mortality findings. Insulin resistance was estimated using the homeostatic model assessment of insulin resistance (HOMA-IR). Multivariable Cox proportional hazards models were used to compute hazard ratios (HRs) with 95% confidence intervals (95% CIs) for quartile comparisons. Outcomes included breast cancer incidence, deaths from breast cancer, and deaths after breast cancer (breast cancer followed by death from any cause). During a median of 19.8 years of follow-up of 1328 breast cancer cases, there were 512 deaths reported, 151 of which were from breast cancer. Breast cancer incidence was higher in women in the highest HOMA-IR quartile (HR, 1.34; 95% CI, 1.12-1.61 [P for trend=.003]). Although HOMA-IR was not found to be associated with risk of death from breast cancer (HR, 1.04; 95% CI, 0.60-1.79), women in the highest versus those in the lowest HOMA-IR quartile were at a higher risk of death after breast cancer (HR, 1.78; 95% CI, 1.32-2.39 [P for trend <.001]). Higher levels of insulin resistance in postmenopausal women are associated with higher breast cancer incidence and higher all-cause mortality after breast cancer.

Agrimonia pilosa Ledeb. Ameliorates Hyperglycemia and Hepatic Steatosis in Ovariectomized Rats Fed a High-Fat Diet

Estrogen deficiency is associated with obesity, dyslipidemia, and increased insulin resistance in postmenopausal women. An efficient therapeutic agent prevents or improves postmenopausal conditions induced by estrogen deficiency. Here, we investigated the effects of aqueous Agrimonia pilosa Ledeb. extract on glucose and lipid metabolism in ovariectomized rats fed a high-fat diet (HFD). Female Sprague-Dawley rats were sham-operated or ovariectomized, and 3 weeks later were assigned to the following groups: sham-operated + HFD (S); ovariectomized + HFD (OVX); and ovariectomized + HFD with 0.5% A. pilosa aqueous extract (OVX + 0.5A) groups. Ovariectomy significantly increased body weight and dietary intake relative to the S group. However, A. pilosa treatment did not significantly affect weight gain or dietary intake. Blood triacylglycerol, total cholesterol, and low-density lipoprotein cholesterol levels tended to decrease in the A. pilosa-supplemented group. Blood glucose levels were significantly lower in the OVX + 0.5A group than those in the OVX group. Blood adiponectin and insulin concentrations increased significantly after A. pilosa treatment in the ovariectomized group. A. pilosa supplementation tended to decrease liver weights and prevented lipid accumulation. These effects correlated with reduced hepatic expression of lipogenesis-related genes (fatty acid synthase, acetyl-coenzyme A carboxylase alpha, and 3-hydroxy-3-methylglutaryl-coenzyme A reductase). Therefore, A. pilosa may improve metabolic disorders in ovariectomized rats.

Marrow adiposity as an indicator for insulin resistance in postmenopausal women with newly diagnosed type 2 diabetes – an investigation by chemical shift-encoded water-fat MRI

BackgroundMarrow fat accumulates in diabetic conditions but remains elusive. The published works on the relationships between marrow fat phenotypes and glucose homeostasis are controversial. PurposeTo detect the association of insulin resistance with marrow adiposity in postmenopausal women with newly diagnosed type 2 diabetes (T2D) using chemical shift-encoded water–fat MRI. MethodsWe measured vertebral proton density fat fraction (PDFF) by 3T-MRI in 75 newly diagnosed T2D and 20 nondiabetic postmenopausal women. Bone mineral density (BMD), whole body fat mass and lean mass were determined by dual-energy X-ray absorptiometry. Insulin sensitivity was estimated using the homeostasis model assessment of insulin resistance (HOMA-IR). ResultsLumbar spine PDFF was higher in women with T2D (65.9 ± 6.8%) than those without diabetes (59.5 ± 6.1%, P = 0.009). There was a consistent inverse association between the vertebral PDFF and BMD. PDFF had a positive association with glycated hemoglobin and HOMA-IR but not with fasting plasma glucose and insulin. PDFF was significantly increased, and BMD was decreased in a linear trend from the lowest (<1.90) to highest (≥2.77) HOMA-IR quartile. Multivariate linear regression analyses revealed a positive association between log-transformed HOMA-IR and PDFF after adjustment for multiple covariates (ß = 0.382, P < 0.001). The positive association of HOMA-IR with PDFF remained robust when total body lean mass and fat mass, BMD was entered into the multivariate regression model, respectively (ß = 0.293 and ß = 0.251, respectively; all P <0.05). ConclusionsElevated HOMA-IR was linked to higher marrow fat fraction in postmenopausal women with newly diagnosed T2D independently of body compositions.

Use of Oral Contraceptives at Child-Bearing Age Are Associated with the Prevalence of Diabetes in Postmenopausal Women

Objective: The effect of past use of oral contraceptives (OCs) at child-bearing on the incidence of diabetes and insulin resistance (IR) after menopause is not clearly elucidated. This study aimed to evaluate the association of past use of OCs with the development of diabetes and IR in post-menopausal women. Methods: This cross-sectional study was based on data from the Korea National Health and Nutrition Examination Survey from 2007 to 2012. Of the 50,4participants, 6554 post-menopausal women were selected and included in the analysis. The long-term effects of OCs use on the prevalence of diabetes in post-menopausal women were examined using multivariate logistic analysis. In addition, fasting glucose and insulin levels were measured in 3338 nondiabetic post-menopausal women, and the association between IR and OCs was examined by analysis of covariance. Results: The prevalence of diabetes was significantly higher in post-menopausal participants who had taken OCs for more than 6 months than in those who had never taken OCs. The association remained significant after adjusting for multiple confounding factors (odd ratio, 1.379; 95% CI, 1.115-1.707; P=0.003). The duration of OCs use was also positively associated with the prevalence of diabetes. Furthermore, taking OCs for more than 6 months led to a significant increase in fasting insulin levels and HOMA-IR in nondiabetic participants. Conclusion: Past use of OCs for more than 6 months led to a significant increase in the prevalence of diabetes in post-menopausal women, and increased IR in nondiabetes participants. These results suggested that prolonged use of OCs at a reproductive age is an important risk factor for developing diabetes in post-menopausal women. Disclosure S. Kim: None. R. Kim: None. E. Jeon: None. J. Lee: None. H. Shon: None.

Ectopic Lipid Deposition Is Associated With Insulin Resistance in Postmenopausal Women.

Menopause is associated with an increased incidence of insulin resistance and diabetes. The aim of this study was to explore the lipid deposition in liver and skeletal muscle and investigate the association with insulin sensitivity in postmenopausal and premenopausal women. Single-center cross-sectional study of 55 healthy women between 45 and 60 years of age. We measured lipid deposition in the liver with magnetic resonance spectroscopy, intramuscular and intra-abdominal lipid deposition with MRI, body composition with a dual-energy X-ray absorptiometry scan, and insulin sensitivity with the composite Matsuda Index. We studied the association between fat distribution, ectopic lipid deposition, and insulin sensitivity in pre- and postmenopausal women. Postmenopausal women had an increased lipid deposition in the liver [0.68% (0.44 to 0.99) vs 0.49% (0.38 to 0.64), P = 0.01] and skeletal muscle [3% (2 to 4) vs 2% (1 to 3), P = 0.001] and had a 28% lower Matsuda insulin sensitivity index during an oral glucose tolerance test (6.31 ± 3.48 vs 8.78 ± 4.67, P = 0.05) compared with premenopausal women. Total fat mass and leg fat mass were stronger predictors of ectopic lipid deposition, and visceral fat mass was a stronger predictor of both ectopic lipid deposition and insulin resistance in postmenopausal women compared with premenopausal women. For a given subcutaneous and visceral fat depot size, postmenopausal women show increased ectopic lipid deposition and insulin resistance compared with premenopausal women. It is suggested that lipid deposition in liver and skeletal muscle may represent important mechanistic links between the changes in fat depots and the increased incidence of insulin resistance seen after menopause.

Effect of Agrimonia Pilosa Ledeb. on glucose tolerance and hepatic steatosis in ovariectomized rats fed high fat diet

Estrogen deficiency is associated with obesity, dyslipidemia, and increased insulin resistance in postmenopausal women. Development of an efficient therapeutic agent without side effects is needed to prevent or improve postmenopausal symptoms and diseases induced by estrogen deficiency. This study was performed to investigate the effects of water extract from Agrimonia pilosa Ledeb. on glucose and lipid metabolism in ovariectomized rats fed high fat diet. Female Sprague Dawley rats were sham operated or ovariectomized and after 3 weeks, assigned to the following groups: sham‐operated + high fat diet(S); ovariectomized + high fat diet (OVX); ovariectomized + high fat diet with 0.1% A. pilosa water extract (OVX+0.1A); ovariectomized + high fat diet with 0.5% A. pilosa water extract (OVX+0.5A). In our results, ovariectomy significantly increased body weight and dietary intake compare with sham group (p&lt;0.05), however there was no significant difference in weight gain and dietary intake by A. pilosa treatment. Blood triglyceride, total‐cholesterol, and LDL‐cholesterol showed a tendency to decrease in A. pilosa supplemented groups. In glucose metabolism, we observed the blood glucose levels of the OVX+0.1A and OVX+0.5A groups were lower than those of the OVX group (p&lt;0.05). Blood adiponectin concentration was also increased significantly by A. pilosa treatment in ovariectomized group (p&lt;0.05). Additionally, we investigated the suppressive effect of A. pilosa aqueous extract on hepatic lipid accumulation. These effects were accompanied by reduced hepatic tissue expression of steatosis‐related genes. These data suggest that A. pilosa improves glucose tolerance and hepatic steatosis in ovariectomized rats.Support or Funding InformationThis study was supported by a research project of the National Institute of Agricultural Science of the Rural Development Administration, Republic of Korea (project no. PJ008554), and by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIT) (No. 2017R1C1B5018328).This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Poor Sleep Quality is Associated with Insulin Resistance in Postmenopausal Women With and Without Metabolic Syndrome.

Poor sleep quality has previously been shown to be related to insulin resistance in apparently healthy adults. However, it is unclear whether an association between sleep quality and insulin resistance exists among adults with metabolic syndrome (MetS). Participants included 347 overweight/obese postmenopausal women without type 2 diabetes (age: 57.5 ± 6.5 years; body mass index [BMI]: 31.7 ± 3.7 kg/m2; 54% with MetS). Sleep quality was assessed with the six-item Medical Outcomes Study Sleep Scale; values were categorized into quartiles. Insulin resistance was calculated from fasting glucose and insulin with the homeostasis model assessment of insulin resistance (HOMA2-IR) method. Analysis of covariance models were used to examine the association between sleep quality and HOMA2-IR after accounting for MetS and covariates (e.g., BMI, cardiorespiratory fitness, and energy intake). Women with the worst sleep quality had significantly higher HOMA2-IR values than women in all other quartiles (P ≤ 0.05 for each), and women with MetS had significantly higher HOMA2-IR values than women without MetS (P < 0.0001), but the relationship between sleep quality and HOMA2-IR did not differ between those with or without MetS (P = 0.26). Women with MetS in the worst quartile of sleep quality had higher HOMA2-IR values than all other women (P < 0.02). Taking >30 min to fall asleep, frequent restless sleep, and frequent daytime drowsiness were each related to higher HOMA2-IR values (each P < 0.04). Sleep quality is an important correlate of insulin resistance in postmenopausal women with and without MetS. Intervention studies are needed to determine whether improving sleep improves insulin resistance in populations at elevated cardiometabolic risk.

A positive association between interleukin-1 receptor antagonist and insulin resistance in postmenopausal women

Objective: The purpose of this study was to determine whether higher circulating interleukin-1 receptor antagonist (IL-1Ra), an anti-inflammatory cytokine, was associated with insulin resistance in postmenopausal women.Methods: We measured IL-1Ra concentrations in 160 naturally postmenopausal women without a history of diabetes mellitus. A Pearson coefficient was computed to assess the relationship between plasma IL-1Ra and homeostasis model assessment of insulin resistance (HOMA-IR). The association between HOMA-IR and IL-1Ra plasma level above the median was assessed by logistic regression. Linear regression was used to explore the determinants of IL-1Ra plasma levels.Results: A significant positive correlation existed between IL-1Ra and HOMA-IR (r = 0.42, p < .0001). The upper-tertile group of HOMA-IR was associated with approximately 4.5-fold increased risk of plasma IL-1Ra level above the median compared with the low-tertile group after adjustments. When multiple correlates were entered into the regression model simultaneously, only Log HOMA-IR remained significantly related to Log IL-1Ra (p = .007).Conclusions: Our results demonstrated a positive association between plasma IL-1Ra and insulin resistance in postmenopausal women. This analysis suggested that insulin resistance was an important determinant of circulating IL-1Ra for these women.

Increased body fat mass explains the positive association between circulating estradiol and insulin resistance in postmenopausal women.

The relationship between circulating estrogen levels and cardiometabolic risk factors such as insulin resistance is unclear in postmenopausal women. High estradiol (E2) levels have been reported to predict increased risk of type 2 diabetes in this population. We aimed to examine associations among estrogen levels, adiposity measurements, and cardiometabolic risk variables including insulin resistance in postmenopausal women. One hundred-one healthy participants (mean ± SD: age 57 ± 4 yr, BMI 27.9 ± 4.8 kg/m2) were included in the analysis. Fifteen plasma steroids or metabolites were measured by liquid chromatography-tandem mass spectrometry. Insulin sensitivity was assessed with a hyperinsulinemic-euglycemic clamp. Body composition and fat distribution were determined with hydrostatic weighing and computed tomography, respectively. Blood lipids and circulating cytokines were also measured. Circulating E2 was positively correlated with all adiposity indexes ( r = 0.62 to 0.42, P < 0.0001) except waist-to-hip ratio. E2 was positively correlated with VLDL-cholesterol, plasma-, VLDL-, and HDL-triglyceride levels ( r = 0.31 to 0.24, P < 0.02) as well as with hs-CRP and IL-6 ( r = 0.52 and 0.29, P < 0.005) and negatively with HDL-cholesterol, adiponectin, and insulin sensitivity ( r = -0.36 to -0.20, P < 0.02). With adjustments for percent body fat, correlations between E2 and metabolic risk variables were no longer significant. Similar results were observed for circulating estrone (E1) and estrone-sulfate (E1-S) levels. In conclusion, circulating estrogen concentrations are proportional to adipose mass in postmenopausal women, although they remain in the low range. Insulin resistance as well as altered blood lipids and cytokines are observed when circulating estrogen levels are high within that range, but these differences are explained by concomitant variation in total adiposity.

Effects of Two Training Modalities on Body Fat and Insulin Resistance in Postmenopausal Women.

Henríquez, S, Monsalves-Alvarez, M, Jimenez, T, Barrera, G, Hirsch, S, de la Maza, MP, Leiva, L, Rodriguez, JM, Silva, C, and Bunout, D. Effects of two training modalities on body fat and insulin resistance in postmenopausal women. J Strength Cond Res 31(11): 2955-2964, 2017-Our objective was to compare the effects of a low-load circuit resistance training protocol and usual aerobic training in postmenopausal women. Postmenopausal women with at least 1 feature of the metabolic syndrome were randomly allocated to a low-load circuit resistance training protocol or traditional aerobic training in a braked cycle ergometer. The intervention consisted in supervised sessions lasting 40 minutes, 3 times per week, during 6 months. At baseline and at the end of the intervention, fasting serum lipid levels, serum interleukin 6, C-reactive protein, 8 isoprostanes, and insulin resistance (assessed through QUICKI and HOMA-IR) were measured. Body fat was measured by double-beam X-ray absorptiometry and by computed tomography densitometric quantification at lumbar 3 vertebral level. Twenty-one women aged 58 (54-59) years were allocated to aerobic training and 21 women aged 55 (52-61) years were allocated to the low-load circuit resistance training protocol. Eighteen and 16 women in each group completed the 6 months training period. Women in both groups experienced significant reductions in blood pressure, total body, subcutaneous, and intraabdominal body fat. Reductions in total cholesterol and triacylglycerol levels were also observed. No changes in insulin resistance indexes, 8 isoprostanes, C-reactive protein, or interleukin 6 were observed in either group. No significant differences between treatment groups were observed in any of the measured parameters. We conclude that low-load circuit resistance training and aerobic training resulted in the same reductions in body fat and serum lipid levels.

Parity and increased risk of insulin resistance in postmenopausal women: the 2010 Korean National Health and Nutrition Examination Survey.

The objective of this study was to assess the association between parity and insulin resistance in nondiabetic, postmenopausal women. This cross-sectional study was conducted using data from the 2010 Korean National Health and Nutrition Examination Survey administered by the Korean Ministry of Health and Welfare. A total of 1,243 nondiabetic postmenopausal women were included in this study and subdivided into three groups according to parity (1-2, 3-4, and ≥5 live births). Insulin resistance was measured using the homeostasis model assessment of insulin resistance (HOMA-IR) index. The relationship between parity and insulin resistance was investigated using analysis of covariance. HOMA-IR showed a positive relationship with parity. Mean HOMA-IR (geometric mean and 95% CI) increased according to increasing parity group (1-2, 3-4, and ≥5 live births) after adjustment for age, smoking, alcohol consumption, exercise, education, income, and body mass index as follows: 2.1 (2.0-2.2) < 2.2 (2.1-2.3) < 2.5 (2.2-2.8) (P = 0.040 and P for trend = 0.012). In addition, this positive association was more apparent when insulin resistance was accompanied by obesity. The mean parity of the obese and insulin-resistant group was significantly higher than that of the nonobese insulin-sensitive group (3.6 ± 0.1 vs 3.2 ± 0.1, P = 0.047). Our study provides the first evidence that parity is significantly associated with insulin resistance in nondiabetic postmenopausal women. Further prospective longitudinal studies are needed to confirm the impact of parity on insulin resistance.

Systematic review and meta-analysis on the effect of exercise on insulin resistance in postmenopausal women: A study from the Health Outcomes and Systematic Analyses (HOUSSAY) group

Background: Insulin resistance is common among aging women. However, the extent of this problem is not clear and there are controversial results regarding the effect of exercise on insulin resistance-related outcomes (IRRO) after menopause.