Insufficient Preservation Research Articles

Photo-responsive Cu-tannic acid nanoparticle-mediated antibacterial film for efficient preservation of strawberries

The existing films used for fruit preservation suffer from insufficient preservation abilities. This study introduces Cu-tannic acid (Cu-TA) nanoparticles, synthesized from tannic acid (TA) and Cu2+, to enhance food packaging properties. Integrated into a chitosan-gelatin (CG) matrix, the resultant Cu-TA nanocomposite films exhibit superior antibacterial efficacy and killing rates of Escherichia coli and Staphylococcus aureus more than 99 %, and double the shelf life of strawberries, underscoring the exceptional freshness preservation capabilities of film. Additionally, the tensile strength of the Cu-TA nanocomposite films increased by 1.75 times, the DPPH radical scavenging percentage increased from 29.4 % to 68.4 %, and the water vapor permeability (WVP) decreased by about 60 % compared to the pure CG films. Comprehensive cytotoxicity and migration assessments confirm the safety of film, paving the way for their application in food packaging. The excellent performance of the Cu-TA nanocomposite films positions them as a formidable solution for protecting perishable food items.

Managing Postharvest Losses of Vegetables and Fruits: A Methodological Review.

Vegetables and fruits are highly perishable agricultural commodities cultivated all over the world. However, inadequate handling practices have led to significant postharvest losses of these agricultural commodities, as well as the wastage of valuable resources, such as time and money. Hence, it can be observed that cultivators often experience significant financial setbacks as a result of inadequate comprehension regarding the nature and origins of these losses, insufficient preservation practices, and ineffective approaches to transportation and marketing. In addition, the utilization of suitable chemical agents during both the pre- and postharvest phases has the potential to prolong the shelf life of agricultural products. This preservation technique safeguards vegetables and fruits from pathogenic organisms and other forms of environmental harm, thereby enabling their availability for an extended duration. Therefore, this review proposes a methodology for managing fruits and vegetables postharvest to minimize losses and optimize returns.

Coordination of Online Shopping Supply Chain Considering Fresh Product Preservation Efforts and Cargo Damage Costs

To reduce the losses caused by insufficient preservation efforts during transportation, the preservation effort level has been the focus of research. In the fierce competition of online sales, it is particularly important to reduce the cost of damaged goods by improving the level of preservation efforts. Therefore, according to Stackelberg game theory, this article establishes five decision-making models and incorporates the damage rate and preservation effort level into the research. Finally, this article coordinates the online shipping supply chain (SC) through a joint contract. After comparing and analyzing the model results, research has found that: (1) in centralized model, the level of preservation effort reaches its optimal level and the system benefit is maximized; (2) under third-party logistics (TPL) leading decision-making, the different bearers of cargo damage costs will not affect the profits of both parties and the system; (3) among the four decentralized models, the level of preservation efforts and system profit are highest when the decision is led by online store and TPL bears the cost of damaged goods; and (4) under a given sharing ratio, when the logistics service quotation satisfies a certain range of condition, the online shopping SC can achieve Pareto improvement. This paper studies the differences and reasons for decision models in the supply and demand relationship between online stores and TPL, which provides fresh product e-commerce decision-makers with a theoretical basis.

On the use of collections with unreliably determined sex and age characteristics in model train-ing for sex determination by traits of the standard craniometric program

The study is concerned with the feasibility of applying machine-learning methods to determine the sex from craniometric features when working with materials from archaeological excavations. A specific feature of such materials is subjectively estimated sex and age characteristics of individuals. The main object of the analysis was a sample measured by V.P. Alekseev and comprised of 258 crania (137 male and 121 female) characterising Russian population of the European part of Russia in the 17th–18th cc. As a test sample, a group of crania of the Russians with documented sex and age, registered within several collections of the Kunstkamera’s repository, also measured by V.P. Alekseev, was used. The series includes 89 male and 10 female skulls, which came to the museum from the Military Medical Academy in 1911–1914 by the effort of the Russian anatomist K.Z. Yatsuta. The models were trained, validated, and tested using four different methods, including discriminant analysis, lo-gistic regression, random forest, and support vector machine. Thirty-three craniometric traits were included in the analysis, from which a group of five features with the highest differentiating ability (Nos. by Martin) — 1, 40, 43, 45, 75(1) — was chosen. When both sets of traits were used for the models commensurable performance indica-tors were obtained. According to the results of the cross-validation, in 85–88 % of cases, on average, all four models accurately predicted the sex estimates given by V.P. Alekseev. When the models were applied to the test sample, the proportion of accurate classifications did not change and stood at 87–88 %. At the same time, the machine-learning methods did not reveal any noticeable advantages in the level of the classification accuracy over the linear discriminant analysis. In general, the efficiency of the obtained models corresponds to the average value of the indicators calculated from the materials of 80 publications (86 %). It is likely that the crania, whose sex cannot be correctly classified neither by the models nor by visual assessment, constitute overlapping sets, which have some common morphological features assimilating them to individuals of the opposite sex. Applica-tion of the models to the skulls of the test sample, re-measured by the author, revealed some deterioration of the model performance indicators in all four cases. The decrease in the proportion of accurate classifications is caused mainly by discrepancies in the estimation of the nasal protrusion angle, as well as subjective errors in the size estimation under insufficient preservation of the crania and partial atrophy of the alveolar process.

Pollen grains associated with Karkenia irkutensis Nosova (Ginkgoales) from the Jurassic of Siberia

Pollen grains found on the nucellar cuticle and the outer cuticle of the integument of Karkenia irkutensis ovules from the Middle Jurassic of the Irkutsk Coal Basin in Siberia were studied with light and transmission electron microscopies. The overwhelming majority of the pollen grains are non-saccate, boat-shaped and monosulcate and can be ascribed to Cycadopites, which fits to what we expected to find in association with ginkgoalean macrofossils. In addition, several non-saccate and bisaccate pollen grains were also detected and interpreted as alien pollen of wind-pollinated conifers. The Cycadopites pollen grains showed from moderate to poor preservation of the exine ultrastructure, that restricted us from decisive conclusions. Differences in the exine ultrastructure among the sectioned Cycadopites pollen grains are probably due to the insufficient preservation and different angles under which the pollen grains were sectioned, and the Cycadopites pollen most probably derived from the same plant. The ectexine is more electron-dense and much thicker than the endexine, which is not always preserved. The ectexine is predominantly homogeneous, but rare cavities of irregular size and shape (=alveoli) are present in a row in the deep layers of the ectexine. Towards the aperture, the ectexine becomes thinner and can acquire a crenulate outer contour (that means a more distinct surface sculpturing). The exine is usually broken over the aperture. This set of characters is more similar to Ginkgo-type of the exine ultrastructure than to Aegianthus- and Sorosaccus sp.-types, which we defined for ginkgoaleans.

ICHTYOCOPRUS AFFECTATUS MAKARENKO, 1993 IS A BIVALVE BORING ROCK CAST, NOT A COPROLITE

The type material of Ichtyocoprus affectatus Makarenko, 1993, originating from the Eocene deposits of the Vizyrka quarry nearthe former town of Ingulets’ (now part of the city of Kryvyi Rih), was studied. This ichnotaxon was originally classified as a fishcoprolite. The results of the study of the specimen figured and described by Dmytro Ye. Makarenko demonstrated that thisfossil is actually a rock cast of a bivalve boring belonging to the ichnogenus Gastrochaenolites Leymerie, 1842. However, it wasnot possible to determine its ichnospecies affiliation due to the insufficient preservation of the material. The unusual surfacepattern of the only specimen of Ichtyocoprus affectatus described by Makarenko is an imprint of a coral substrate, rather thana structure that can occur in the gut of a coprolite producer.

SAFEGUARDING CULTURAL HERITAGE SITE: COMPARATIVE STUDY FOR ESTABLISHING CONSERVATION INITIATIVES ON INDRAPATRA FORT, ACEH BESAR

The Indrapatra Fort cultural heritage site located in the coastal area of Aceh Besar, Indonesia is a relic of Hindu influence. The condition of the fort is decreasing, so that it has a negative impact on securing this cultural heritage site. The lack of a maintenance strategy, continued neglect, and insufficient preservation work by the relevant government conservation agencies have resulted in almost no conservation initiatives for the fort. This paper attempts to establish appropriate conservation initiatives for fort security. This was carried out based on a qualitative approach with a comparative study of local cultural heritage conservation initiatives at Borobudur Temple and Fort Rotterdam. Primary and secondary data were analyzed using descriptive qualitative methods, providing recommendations for initiatives to preserve Indrapatra Fort that could be implemented practically through tangible and intangible aspects. Tangible initiatives are the reconstruction of damaged fortification structures and elements with similar materials, combined with a sound maintenance strategy. Meanwhile, intangible initiatives include performing arts, annual festivals and providing guides.

Genotyping and phylogenetic analysis of free-living amoeba (Acanthamoeba and Naegleria) in treated and untreated water in the northeastern provinces of Iran

Abstract Free-living amoebae (FLA) are widely distributed protozoa in natural or man-made aquatic environments without the need for a host organism for survival. Several strains of FLA are known to be pathogenic. As of date, there is inadequate data on the geographical distribution of FLA in northeastern and northern Iran. This study aimed to investigate the prevalence and genotype distribution of Acanthamoeba and Naegleria in drinking water and surface water samples in northern and northeastern Iran. A total of 60 water samples were collected and filtered from various sources for the presence of amoebae. DNA extraction was performed, and PCR confirmed the presence of FLA. PCR products were sequenced to identify the species/genotype. Phylogenetic relationships and taxonomic status constructed using MEGA X software. The findings on growth media showed 35% (21/60) and 26% (16/60) were positive for Acanthamoeba and Naegleria, respectively, while PCR analysis also obtained similar results. All isolates of Acanthamoeba were identified as T4 genotype. Poor water quality, as well as insufficient preservation and treatment, might indicate that chlorine disinfection is ineffective in removing contamination of amoebas in treated water samples. Therefore, regular water quality monitoring is essential to control amoeba's growth, reducing the risk of human infections with FLA.

Influence of Pre-Harvest Gibberellic Acid and Post-Harvest 1-methyl Cyclopropane Treatments on Phenolic Compounds, Vitamin C and Organic Acid Contents during the Shelf Life of Strawberry Fruits.

In recent years, significant portions of the fresh fruits and vegetables produced worldwide have been decaying before reaching the consumer because of insufficient preservation after harvest. In this direction, we carried the study out to investigate the effect of gibberellic acid (GA3) and 1-methyl cyclopropane (1-MCP) applications on phenolic compounds and organic acid contents of the strawberry fruits (cv. Albion) during shelf-life. Gibberellic acid treatments, which prepared in two different concentrations (50 and 100 ppm), were performed by spraying the leaves before harvest. 1-methyl cyclopropane applied after harvest. The results of the study showed a greater decrease in organic acids (except oxalic and succinic acid) in Gibberellic acid-applied fruits during shelf-life. Citric acid was recorded as the most abundant organic acid in the control group. In phenolic compounds, gallic acid (15.22 mg 100 g−1) and ellagic acid (9.38 mg 100 g−1) were recorded as the highest phenolic compounds on the third day. 1-MCP and GA3 (50 ppm) + 1-MCP treatment reduced the breakdown of vitamin C during the shelf-life of strawberry fruits compared to the control group. As a result, phenolic compounds, vitamin C, and organic acids decreased during the shelf-life, and 1-MCP applications slowed down the breakdown of these compounds.

Notes on rhopalosomatid wasps of Dominican and Mexican amber (Hymenoptera: Rhopalosomatidae) with a description of the first fossil species of <i>Rhopalosoma</i> Cresson, 1865

Abstract. Rhopalosomatidae are a family of aculeate wasps that are ectoparasitoids of crickets as larvae and are predominantly distributed pantropically. The published fossil record of the family is scarce. Here, we report three new fossil rhopalosomatid wasp specimens from Dominican and Mexican amber. Rhopalosoma hispaniola Lohrmann sp. nov. is described and documented from Dominican amber by two separate inclusions – one of each sex. An additional fossil female Rhopalosoma is described and documented from Mexican amber but is not named due to the insufficient preservation of the fossil. The new fossils, which are morphologically intermediate between Townes' isopus and poeyi species groups, do not only represent the first fossil records of an extant genus of this peculiar family but also the first records of the family in Dominican and Mexican amber.

Fish and Fish Products in Late Antique Palestine and Babylonia in Their Social and Geographical Contexts: Archaeology and the Talmudic Literature

This paper looks at the ancient Jewish talmudic literature (4th-7th centuries CE) for information about fish and fish products in the ancient world and compares it with information from classical sources and from archaeology. Analysis of the texts show that information about food, and particularly fish and fish products, can be derived from texts originally written as religious regulations and moral narratives. Talmudic textual evidence, backed up by parallel archaeological finds, documents long-distance transport of preserved fish, including what is identified as Nile perch from Egypt and mackerel from Spain. Parallel papyrological evidence testifies to the import of the parrot wrasse. Graeco-Roman allec, a sauce made of macerated tiny fish, is identified by the Palestinian Talmud with tarit terufah, made of tiny chopped fish, and afitz, cf Greek afye, immature fish, often cooked as such. Remains of allec have been found archaeologically in Israel/Palestine. The Palestinian Talmud identifies tarit[a] with tzaḥana, one of a trio of strong-smelling Babylonian fish-dishes eaten by the poor: the others are gildana and harsana. The varied material on these discussed here adds to our knowledge of ancient salted and/or fermented fish-dishes. The identification of tzaḥana with 9th-to-10th century ṣaḥna from Baghdad of the Caliphs and 14th-century Cairo appears very likely. Thus tarit/tzaḥana/ṣaḥna appears to have been not very appetising processed fish food for the poor in Roman Palestine, Jewish Babylonia, Baghdad of the Abbasid Caliphs and Mamluk Egypt, and it may be identified with allec. The talmudic literature also discusses reasons for failure of the preservation processes, as well as of potentially lethal effects of insufficient preservation.

'Rolling' stoneflies (Insecta: Plecoptera) from mid-Cretaceous Burmese amber.

This contribution describes seven new species of fossil stoneflies from Cretaceous Burmese amber, all of which are dedicated to present and past members of the Rolling Stones. Two species—Petroperla mickjaggeri gen. nov. sp. nov. and Lapisperla keithrichardsi gen. nov. sp. nov.—are placed in a new family Petroperlidae within the stemline of Systellognatha. The first Cretaceous larval specimen of Acroneuriinae, Electroneuria ronwoodi gen. nov. sp. nov., is also described along with another four new species that are placed within the Acroneuriinae genus Largusoperla Chen et al., 2018: Largusoperla charliewattsi sp. nov., Largusoperla brianjonesi sp. nov., Largusoperla micktaylori sp. nov., and Largusoperla billwymani sp. nov. Additional specimens of Acroneuriinae are described without formal assignment to new species due to insufficient preservation. Implications for stonefly phylogeny and palaeobiogeography are discussed.

Middle Triassic (Anisian, Bithynian) gastropods from North Dobrogea (Romania) and their significance for gastropod recovery from the end‐Permian mass extinction event

AbstractA new early middle Anisian (Bithynian) gastropod fauna is described from the Mahmudia quarry in North Dobrogea, Romania. At least 22 species are reported, nearly all of them from Tubiphytes‐limestones which represent a microbial buildup. Twenty species from Tubiphytes‐buildup limestones are characterized, ten of which in open nomenclature due to insufficient preservation. One genus (Murchisonietta gen. nov) and ten species are described as new. The new species are Euryalox applanatus, Rasatomaria multistriata, Sisenna inaequistriata, Worthenia (Worthenia) dobrogeana, Worthenia (Humiliworthenia) microstriata, Worthenia (Humiliworthenia) anisica, Marmolatella iordanae, Ladinaticella simionescui, Murchisonietta acuta and Coelostylina micropunctata. The present fauna is one of the most diverse Anisian gastropod faunas known to date and sheds light on the pattern of ongoing recovery from the end‐Permian mass extinction event. It is dominated by Neritimorpha, Caenogastropoda and Pleurotomariida both in abundance and number of species. Diversity of Pleurotomariida strongly exceeds that of any known Early Triassic gastropod fauna and suggest a pronounced rebound of this group. High‐spired smooth caenogastropods (Omphaloptycha and Polygyrina) are relatively abundant but for the most part not enough well preserved for species identification. The present gastropod fauna is part of a diverse invertebrate fauna probably comprising more than 50 species with gastropods being the most diverse followed by bivalves and brachiopods. They were microbial‐buildup dwellers while metazoan frame‐builders, especially corals, were still absent as consequence of the end‐Permian mass extinction event. The Mahmudia fauna suggests that metazoan reefs recovered more slowly than benthic faunas.

Neogene Deep-sea Smelts (Argentiniformes: Microstomatidae) from the Far East Russia

Deep-sea smelts (Argentiniformes: Microstomatidae, Bathylaginae) from the Miocene of Far East Russia (Sakhalin and Urup islands) are described. The specimens described belong to four taxa, one of which is assigned to a new species of the genus Leuroglossus. Like Recent smoothtongues, the new species has thickened medial rays in the caudal fin. The small number of abdominal vertebrae makes the new species similar to the extant southern smoothtongue L. stilbius, which occurs along the eastern coasts of the Pacific Ocean. The new species differs from extant smoothtongues in the short snout, which is not longer than the orbit. Phylogenetic analysis of morphological features of the new species, using a matrix of characters of living deepsea smelts, shows that it occupies a basal position in the genus Leuroglossus. Three other extinct taxa cannot be reliably classified below the level of the subfamily Bathylaginae or tribe Bathylagini because of insufficient preservation of available specimens. The new records of deep-sea smelts in Far East Russia show that this fish subfamily was widespread and highly morphologically differentiated in the Neogene.

Hypoanthraconaia: a new genus of non-marine bivalve molluscs from the Early Permian of Far East Russia

Early Permian continental deposits include a large number of localities containing anthracosiid-like non-marine bivalves traditionally assigned to Anthraconaia Trueman and Weir, 1946, Palaeanodonta Amalitzky, 1895, and Palaeomutela Amalitzky, 1892. In most cases, these classifications are only tentative due to insufficient preservation in which the shells are missing their main characteristics: the ligament and the hinge. Non-marine bivalves from the Early Permian Upper Pospelovka Subformation of Russky Island (South Primorye, Far East Russia), described here as Hypoanthraconaia gen. nov., differ morphologically from the above genera by a set of external features including the initial shell, the mode of intersection of the growth lines with the dorsal margin, and the details of the sculpture. Hypoanthraconaia gen. nov. shows the most external similarity with “atypical” anthracosiid-like morphotypes of Anthraconaia that are widespread in the Late Pennsylvanian and Early Permian of eastern North America, and the Stephanian and Early Permian Lower Rotliegend of northwestern Europe. On this basis, the new genus is conventionally assigned to the family Naiaditidae Scarlato and Starobogatov, 1979.

Presto lift\u2014a facelift that preserves the retaining ligaments and SMAS tethering

BackgroundProducing youthful facial appearance by face-lifting often comes along with an undesired loss of patient’s individual phenotype. This may result from insufficient preservation of retaining ligaments, the “guardians of facial identify,” and from severance of the intersegmental connections of the superficial musculo-aponeurotic system (SMAS), which tether, structure, and compartmentalize facial soft tissue into defined, relevant anatomical zones.MethodsThe technique reported here preserves most retaining ligaments. They serve to fix the facial soft tissue mass in loco. With the possible exception of the zygomatic-cutaneous ligament, they are only carefully distended. The SMAS intersegmental connections and the zygomatic SMAS border are preserved to retain effective points of facial tissue fixture. Aging-associated thinning and lengthening of the lower eyelid are reduced by midfacial-submalar preparation (Aston 1996). Subplatysmal preparation and disconnection of the cranial-platysmal border permits optimal modeling of neck structure.ResultsThe combination of preservation of retaining ligaments and SMAS tethering (“PRESTO facelift”) introduced here as a novel face-lifting technique conserves the individual esthetics of the patient by approaching her/his individual phenotype from decades ago. In addition, undesired outcomes of facelift surgery and common risks of facelift surgery are circumvented.ConclusionsThe PRESTO facelift technique generates optimal esthetic results that conserve a patient’s personal facial identity, besides restoring a more youthful appearance and being rapid and safe.

The first elateroid beetles (Coleoptera: Polyphaga: Elateroidea) from the Upper Jurassic of Australia.

The first elateroid fossils from the Upper Jurassic Talbragar Fish Bed in Australia are described and illustrated. Wongaroo amplipectorale gen. et sp.n., based on two specimens, is placed in the family Cerophytidae due to its convex, posteriorly weakly angled and laterally carinate pronotum obscuring the head in dorsal view, its relatively long, pointed elytra and slender legs, its 9-striate elytra with deep basal pits and the absence of metacoxal plates. Beattieellus jurassicus gen. et sp.n., described from one specimen, possesses the acutely angled pronotum without a carina on the posterolateral angles and the ventral click apparatus typical of Eucnemidae and is classified in this family. Assignment of it to a eucnemid subfamily is impossible because of the insufficient preservation of relevant characters in the fossil. Four other elateroid fossils, possibly representing eucnemids and elaterids, are illustrated and briefly described but not named, due to their insufficient preservation. These fossils represent the first of their kind in Australia and in the Southern Hemisphere, and Beattieellus is also the oldest eucnemid fossil known and extends the fossil record of Eucnemidae into the Upper Jurassic. The discovery of elateroid fossils in the Talbragar Fish Bed adds to the coleopteran diversity of this ancient lake ecosystem, indicating that it was well wooded and provided suitable habitats of rotten wood for the development of the larvae of these taxa.

Systematics, phylogeny, and taphonomy of ghost shrimps (Decapoda): a perspective from the fossil record

Ghost shrimps of Callianassidae and Ctenochelidae are soft-bodied, usually heterochelous decapods representing major bioturbators of muddy and sandy (sub)marine substrates. Ghost shrimps have a robust fossil record spanning from the Early Cretaceous (~ 133 Ma) to the Holocene and their remains are present in most assemblages of Cenozoic decapod crustaceans. Their taxonomic interpretation is in flux, mainly because the generic assignment is hindered by their insufficient preservation and disagreement in the biological classification. Furthermore, numerous taxa are incorrectly classified within the catch-all taxon Callianassa. To show the historical patterns in describing fossil ghost shrimps and to evaluate taphonomic aspects influencing the attribution of ghost shrimp remains to higher level taxa, a database of all fossil species treated at some time as belonging to the group has been compiled: 250 / 274 species are considered valid ghost shrimp taxa herein. More than half of these taxa (160 species, 58.4%) are known only from distal cheliped elements, i.e., dactylus and / or propodus, due to the more calcified cuticle locally. Rarely, ghost shrimps are preserved in situ in burrows or in direct association with them, and several previously unpublished occurrences are reported herein. For generic assignment, fossil material should be compared to living species because many of them have modern relatives. Heterochely, intraspecific variation, ontogenetic changes and sexual dimorphism are all factors that have to be taken into account when working with fossil ghost shrimps. Distal elements are usually more variable than proximal ones. Preliminary results suggest that the ghost shrimp clade emerged not before the Hauterivian (~ 133 Ma). The divergence of Ctenochelidae and Paracalliacinae is estimated to occur within the interval of Hauterivian to Albian (133–100 Ma). Callichirinae and Eucalliacinae likely diverged later during the Late Cretaceous (100–66 Ma), whereas Callianassinae did not appear before the Eocene (56 Ma).

Preservation of the brain in cadavers embalmed according to the Thiel protocol (913.2)

Embalming according to the method developed by Walter Thiel has been well accepted world‐wide for practical teaching and training of surgical procedures, as this embalming procedure preserves the softness and elasticity of the tissues. However, this method has the disadvantage of the insufficient preservation of the brain. Walter Thiel has therefore proposed additional intracranial in situ fixation of the brain (W. Thiel, Ann Anat 2002, 184, 267‐9). As the brain is usually taken separately for teaching of neuroanatomy and neurohistology, or for research purpose, we explored the possibility of taking the brain before embalming the body using the Thiel protocol. 4 cadavers were used for this preliminary study. Firstly, the brain was gently excised from the cranial cavity after an extended craniotomy, including the dura mater, the pituitary gland and the medulla oblongata. Thereafter, the internal carotid and vertebral arteries were ligated and the body was perfused using the Thiel protocol. There was no substantial leakage of embalming liquid and the bodies could be used later on for teaching and training procedures. The brains were further processed. They were either freshly processed for research purpose, or fixed in 10% formaldehyde and used either for teaching in the neuroanatomy course or for histology. In conclusion, we recommend that the brain may be taken and processed separately from bodies that are embalmed with the Thiel protocol, especially when human brains are needed for teaching and research purpose.

Ultrasound image anisotropic diffusion de-speckling method based on Mallat-Zhong discrete wavelet transform wavelet

In view of speckle noise in ultrasound image, there are some disadvantages of traditional anisotropic diffusion methods, such as in-sufficient noise suppression and edge details preservation. A de-speckling method based on Mallat-Zhong Discrete Wavelet Transform( MZ-DWT) wavelet was proposed. The method used MZ-DWT wavelet and Expectation Maximization( EM) algorithm as the discrimination factor between homogeneous and edge regions, making it more accurately to control diffusion intensity and rate and achieving the noise suppression and details preservation. The experimental results show that, the proposed algorithm can better de-speckle while preserving image details and the performance of the method is better than the traditional anisotropic diffusion methods.