Insoluble Acid Research Articles

An Ethnobotanical Survey, Pharmacognostic Profile and Phytochemical Investigation of Chrysobalanus icaco L.

The spice known as Chrysobalanus icaco L. (Chrysobalanaceae) was found near the coast of the Niger Delta in Nigeria. A survey on the ethnobotany of C. icaco seeds was conducted in Warri, Abraka, Delta State, Ezetu village, and Onitsha. Standard techniques were used to determine the pharmacognostic profile, phytochemical screening, physiochemical parameters, and elemental analysis. The findings showed that the Ezetu village people utilized the seeds traditionally for stomachache, anti-diarrhea, and post-child delivery. Histochemical analysis revealed the presence of tannins and proteins in the seed. The seed powder's physicochemical parameters are as follows: pH (1 g/100 ml distilled water) (6.00±0.00) and pH (10 g/100 ml), water-soluble ash (1.5±0.00%), alcohol soluble ash (1.0±0.00%), acid insoluble acid (0.25±0.00%), acid soluble ash (1.0±0.00%), and sulfated ash (2.0±0.00%). The seed oil's physicochemical properties were: density (0.936±0.0%), refractive index (1.491±0.00%), iodine value (15.9±0.01%), peroxide value (25.31±0.01%), acid value (29.44±0.00%), and ester value (34.75±0.21%). The elements discovered in the seed included lead, copper, nickel, chromium, cadmium, potassium, sodium, calcium, phosphorus, magnesium, iron, and zinc. Phytochemicals found in the extract include reducing sugars, proteins, amino acids, fats, oils, alkaloids, tannins, phenolic compounds, flavonoids, cholesterol, steroids, terpenoids, triterpenoids, phytosterols, saponins, and cardiac glycosides. Quantitative phytochemical results include total phenolics (11.63±0.03), total flavonoids (2.35±0.06), total alkaloids (5.50±0.03), and total tannins (12.48±0.01). Consequently, it is possible to verify the authenticity of the seeds using these pharmacognostic features.

Antibacterial activity of tekelan leaf ethanol extract (Chromolaena odorata (L.) R. M. King and H. Rob) against of Staphylococcus aureus bacteria

Background: Indonesia is a tropical country, so the prevalence of infectious diseases caused by bacteria still remains high. On the other hand, the intense use of antibacterial agent in Indonesia causes a tendency for bacterial resistance to existing antibacterial drugs. One of the herbal plants that is widely used by some people is tekelan leaves. The purpose of this study was to determine the activity of an ethanol extract of tekelan leaves on the growth of Staphylococcus aureus bacteria. Methods: Simplisia of tekelan leaves was tested for secondary metabolites and simplisa characterization testing. Antibacterial activity testing is carried out by the disc paper method, where the disc paper is immersed in the test solution with a comparison of the test concentration. Results: The results of phytochemical screening show that the secondary metabolites of tekelan leaves are alkaloids, flavonoids, steroids/triterpenoids, tannins, and glycosides. The results of the characterization of simplisia moisture content are 7.99%, water-soluble juice content is 13.29%, ethanol-soluble juice content is 12.18%, total ash content is 7.45%, and insoluble acid ash content is 0.92%. The observation of the antibacterial activity of the ethanol extract of tekelan leaves was carried out by the disc diffusion method. The test results showed that the ethanol extract of tekelan leaves was categorized as moderate at concentrations of 1% and 2% and as strong at concentrations of 4%, 5%, 10%, 20%, 40%, 60%, 80%, and 100%. The conclusion of this study is that ethanol extract had activity to inhibit the growth of Staphylococcus aureus and the MIC value obtained 0.7%. Conclusions: The conclusion of this study is that ethanol extract had activity to inhibit the growth of Staphylococcus aureus.

Physical and Chemical Characteristics of Alginate Extracted from Sargassum sp

Scientists have proved alginate which is extracted from brown seaweed, to be able to reduce blood glucose levels in mice. These results indicate that alginate has health benefits. Sargassum sp. is a brown seaweed growing wildly in Indonesian waters and is considered a prospective source of alginate to be developed and used for various purposes. This study aimed to explore the potency of alginate production using Sargassum sp. as raw material, particularly to reveal the physical and chemical properties of alginate obtained. Alginate was extracted using a method developed by Ref. [1]. During the extraction process, alginate yield was determined. The alginate was analyzed for physical parameters (whiteness degree and viscosity) and chemical parameters (moisture content, acid insoluble ash content, ash content, fat content, and protein content). Results showed that the yield of alginate was 20.30%. The whiteness degree and viscosity values of alginate were -31.53 and 47.25 cPs, respectively. At the same time, the moisture content, insoluble acid content, ash content, fat content, and protein content of alginate were 11.76%, 2.28%, 27.55%, 0.18%, and 0.76%, correspondingly. The alginate was classified as low viscosity and suitable for processing various foods, including functional food, by taking the health benefits of alginate.

Identification and expression analysis of invertase family genes during grape (Vitis vinifera L.) berry development under CPPU and GA treatment.

Sugar is crucial for grape berry, whether used for fresh food or wine. However, berry enlargement treatment with forchlorfenuron (N-(2-chloro4-pyridyl)-N'-phenylurea) (CPPU, a synthetic cytokinin) and gibberellin (GA) always had adverse effects on sugar accumulation in some grape varieties, especially CPPU. Therefore exploring the molecular mechanisms behind these adverse effects could provide a foundation for improving or developing technology to mitigate the effects of CPPU/GA treatments for grape growers. In the present study, invertase (INV) family, the key gene controlling sugar accumulation, was identified and characterized on the latest annotated grape genome. Their express pattern, as well as invertase activity and sugar content, were analyzed during grape berry development under CPPU and GA3 treatment to explore the potential role of INV members under berry enlargement treatment in grapes. Eighteen INV genes were identified and divided into two sub-families: 10 neutral INV genes (Vv-A/N-INV1-10) and 8 acid INV genes containing 5 CWINV (VvCWINV1-5) and 3 VIN (VvVIN1-3). At the early development stage, both CPPU and GA3 treatment decreased the hexose level in berries of 'Pinot Noir' grape, whereas the activity of three types inverstase (soluble acid INV, insoluble acid INV, and neutral INV) increased. Correspondingly, most of INV members were up-regulated by GA3 /CPPU application at least one sampling time point during early berry development, including VvCWINV1, 2, 3, 4, 5, VvVIN1, 2, 3 and Vv-A/N-INV1, 2, 5, 6, 7, 8, 10. At maturity, the sugar content in CPPU-treated berries is still lower than that in the control. Soluble acid INV and neutral INV, rather than insoluble acid INV, presented lower activity in CPPU-treated berries. Meanwhile, several corresponding genes, such as VvVIN2 and Vv-A/N-INV2, 8, 10 in ripening berries were obviously down-regulated by CPPU treatment. These results suggested that most of INV members could be triggered by berry enlargement treatment during early berry development, whereas VvVINs and Vv-A/N-INVs, but not VvCWINVs, could be the limiting factor resulting in decreased sugar accumulation in CPPU-treated berries at maturity. In conclusion, this study identified the INV family on the latest annotated grape genome and selected several potential members involving in the limit of CPPU on final sugar accumulation in grape berry. These results provide candidate genes for further study of the molecular regulation of CPPU and GA on sugar accumulation in grape.

Standardization And Analytical Evaluation Of Siddha Herbal Formulation Aavarai Kirutham In The Treatment Of Madhumega Avathai Vii: Diabetic Peripheral Neuropathy

Aavaarai kirutham is a Siddha herbal confection which literarily recommended for the treatment of diabetic peripheral neuropathy (Mathumega Avathaigal) in the authentic Siddha textbook " Sigicha rathina deepam". There are no standards for this antidiabetic herbal preparation. Standardization of the individual ingredients helps to prepare a quality test drug which responsible for the desired pharmacological effect. So, the present study has been performed to standardize unique ingredients and the herbal formulation 'Aavaarai kirutham' as a preliminary study by using three groups of test drug samples. The parameters used to standardize the preparation are organoleptic characters, Biochemical analysis, Physicochemical properties,HPTLC fingerprint analysis, Heavy metal analysis by ASS, Test for specific pathogens, Sterility test by pour plate method, Aflotoxin analysis, Pesticide residue analysis,and Thin layer chromatography. The organoleptic characteristics such as color, taste, odor, smell, and consistency of the ingredients and the test drug were evaluated. Physico-chemical parameters were determined as per WHO guidelines and reported as total ash, insoluble acid ash, water soluble extractive, ethanol soluble extractive, total sugar, reducing sugar content, fat content, and loss on drying. Thin layer chromatography developed for different solvent systems and detection of TLC fingerprint profiles were carried out under UV light at the wavelength of 366nm and also viewed after spraying with anisaldehyde sulfuric acid spray reagent. Colors and Rf Values of major spots were noted.

The effects of arbuscular mycorrhizal fungi and deficit irrigation on the yield and sugar content of watermelons (Citrullus lanatus)

Many studies have demonstrated that arbuscular mycorrhizal fungi (AMF) and deficit irrigation (DI) have positive effects on the fruit yield or quality. This study aims to test whether the watermelon sugar content and yield can be improved by appropriate amounts of AMF and DI methods and to clarify the underlying physiological mechanism. Diploid and triploid watermelon cultivars and their pumpkin rootstock-grafted seedlings were treated with AMF, DI and DI + AMF in a randomised complete block design with five replications. The results showed that DI significantly reduced the relative water content (RWC), PN, alkaline α-galactosidase activity, but increased the insoluble acid invertase (IAI), sucrose synthase (SuSy) and sucrose phosphate synthase (SPS) activities compared with the well-watered (WW) treatment, which led to a decrease in the fruit yield and an increase in the fruit sugar content. Although the AMF improved the RWC, PN and alkaline α-galactosidase, IAI, SuSy and SPS activities in all the watermelon lines under both the DI and WW conditions, the improvement magnitude of these parameters was more pronounced in the pumpkin-root watermelon lines than the corresponding own-root watermelon lines, especially under the DI condition. The integrated application of AMF and DI increased the fruit yield to a level similar to the WW value in the pumpkin-root watermelon lines and sugar content to an optimal level in all the watermelon lines.

Antibacterial activity test of solid soap with torch ginger's (Etlingera elatior (Jack) R.M.sm.) flower extract

Ethanol extract of torch ginger’s flower (Etlingera elatior) is rich in active compounds such as flavonoids, alkaloids, and saponins, steroids. Flavonoids are bioactive compounds that serve as antioxidants and antibacterials. This study aims to find the antibacterial activity of solid soap with torch ginger’s flower extract against S. aureus and P. aeruginosa. This study includes the preparation of simplicia, extracts, moisture content, total ash levels, insoluble acid ash levels, water soluble extract levels. The first evaluation includes organoleptic test, pH, irritation, and stability. The formulation of the tested soap preparations was 5%, 7.5%, 10%. The data analysis was performed with ANOVA. The results of this study revealed that its ethanolic extract had a moderate category of bacterial resistance against S.aureus where in FI (5%) has a clear zone with 7 mm in diameter, FII (7.5%) 8.3 mm and FIII (10%) 10.4 mm.Furthermore,for P.aeruginosa, it has a moderate clear zone with diameter in FI (5%) is 8.86 mm, FII (7.5%) is 9.73 mm, and FIII is 10.6 mm. Torch ginger’s flower ethanolic extract had anantibacterial activity against S.aureus and P.aeruginosa with the widestclear zone is in the FIII (10%, v/v) in the number of 10.4 mm against S.aureus and 10.6 mm against P.aeruginosa with moderate resistance category.

Pharmacognostic standardization and qualitative analysis of Gymnosporia senegalensis

This study aims to evaluate the pharmacognostic parameters of different parts of Gymnosporia senegalensis (Lam.) Loes. These parameters play a vital role in the traditional medicine system for administering the drug and their therapeutic effects on various ailments. Standardization parameters include sequential extract preparation, physicochemical studies (ash value, moisture content, pH, fluorescence analysis), and phytochemical screening of the three parts of G. senegalensis. Various macro and microelements (in ppm) were estimated by using atomic absorption spectroscopy (AAS). Total ash content (3.04±0.02), water-soluble ash (2.63±0.01), and insoluble acid ash (0.72±0.01) were found to be the highest in the leaf. Foreign organic matter was observed higher in stem (0.07±0.04) followed by bark and leaf. The moisture content was found lowest in bark (2.02±0.01), preventing microbial growth. The pH of the crude drug of plant parts is weakly acidic, ranging from pH 5.33 to 5.96 at 1 % and 4.93 to 6.03 at 10 %. Fluorescence results were also helpful in detecting the substituents and adulterants and assessing the crude drugs qualitatively. The present study revealed the presence of various primary and secondary metabolites (terpenoids, flavonoids, tannins, and saponin) in high and moderate amounts in the extracts of different parts of the plant. The stem and bark also showed a reasonable presence of macro and microelements (As, Cr, Cd, Pb, Zn, Mn, and Cu). The complete analysis provides valuable information for the quality assurance of G. senegalensis as a crude drug for preparing formulations of herbal medications.

Pengaruh Lama Penyimpanan dan Pengenceran Lindi Hitam Terhadap Karakteristik Lignin Kraft Acacia mangium

This study aimed to investigate the effects of storage time and dilution of black liquor (BL) from Acacia mangium kraft pulping on the characteristics of isolated Lignin. Lignin isolation was carried out by 1 and 2 steps of isolation using HCL 1M to precipitate Lignin, diluted before isolation. Isolated Lignin was analyzed for its acid-soluble Lignin (ASL), insoluble acid lignin (AIL), functional groups by FTIR, solubility in dioxane and NaOH and thermal properties. The effect of BL storage time was also evaluated on the characteristics of the Lignin produced. The results suggest that the longer BL is stored, the higher the lignin yield. When compared to the isolation approach without dilution, the dilution process produced a higher yield and ash content up to 84% and 21%, respectively. Without dilution, the AIL isolated was lower than the dilution during BL storage. The longer the storage duration, the higher the lignin purity. Compared to two-step lignin isolation, dilution treatment in single-step isolation improves yield and purity. The thermal stability of lignin isolation without dilution (184 ⁰C and 167 ⁰C for 1 and 2 steps, respectively) was higher than that of isolated Lignin with dilution (154.8 ⁰C and 160.9 ⁰C for 1 and 2 steps, respectively), according to thermal study. Both lignin isolates with and without dilution have comparable functional groups, as shown by FTIR spectra. Due to the high yield and purity of isolated Lignin, BL dilution could be a viable alternative in lignin isolation from BL. Moreover, the properties of isolated Lignin are also influenced by BL storage.

Comparative Pharmacognostical and Phytochemical Account of the Peel and Pulp of the Fruit of C. pepo

The current investigation was performed to explore the phytochemicals and pharmacognostic activity in the pulp and peel part of the fruit of Cucurbita pepo. The most important stage in determining the identification of a crude medicine and assessing its quality and purity is pharmacognostic assessment. The parameters such as acid-insoluble, water-soluble, total ash value, extractive value, and moisture content were determined. Fluorescence characterization and phytochemical screening were also done to know the presence of various phytocompounds. The phytochemical investigation revealed therapeutic components in the peel and pulp extracts of C. pepo. The ash value of peel and pulp was 6.9 ± 0.17 and 5.8 ± 0.07, the insoluble acid value was 2.9 ± 0.10 and 1.7 ± 0.05, and the water-insoluble value was 3.6 ± 0.41 and 2.4 ± 0.10 respectively. The moisture content of peel and pulp was determined to be 0.249±0.022gm and 0.256±0.025 gm, respectively, while the extractive values were observed as 23.71±1.25 in methanolic extract of peel whereas 55.44±2.019 in aqueous extract of pulp.

Physical and Chemical Properties of Acacia mangium Lignin Isolated from Pulp Mill Byproduct for Potential Application in Wood Composites.

The efficient isolation process and understanding of lignin properties are essential to determine key features and insights for more effective lignin valorization as a renewable feedstock for the production of bio-based chemicals including wood adhesives. This study successfully used dilute acid precipitation to recover lignin from black liquor (BL) through a single-step and ethanol-fractionated-step, with a lignin recovery of ~35% and ~16%, respectively. The physical characteristics of lignin, i.e., its morphological structure, were evaluated by scanning electron microscopy (SEM). The chemical properties of the isolated lignin were characterized using comprehensive analytical techniques such as chemical composition, solubility test, morphological structure, Fourier-transform infrared spectroscopy (FTIR), 1H and 13C Nuclear Magnetic Resonance (NMR), elucidation structure by pyrolysis-gas chromatography-mass spectroscopy (Py-GCMS), and gel permeation chromatography (GPC). The fingerprint analysis by FTIR detected the unique peaks corresponding to lignin, such as C=C and C-O in aromatic rings, but no significant differences in the fingerprint result between both lignin. The 1H and 13C NMR showed unique signals related to functional groups in lignin molecules such as methoxy, aromatic protons, aldehyde, and carboxylic acid. The lower insoluble acid content of lignin derived from fractionated-step (69.94%) than single-step (77.45%) correlated to lignin yield, total phenolic content, solubility, thermal stability, and molecular distribution. It contradicted the syringyl/guaiacyl (S/G) units’ ratio where ethanol fractionation slightly increased syringyl unit content, increasing the S/G ratio. Hence, the fractionation step affected more rupture and pores on the lignin morphological surface than the ethanol-fractionated step. The interrelationships between these chemical and physicochemical as well as different isolation methods were investigated. The results obtained could enhance the wider industrial application of lignin in manufacturing wood-based composites with improved properties and lower environmental impact.

EFFICACY OF AQUEOUS AND METHANOLIC EXTRACTS OF PLANT BRASSICA RAPA LEAVES POTENTIAL ANTI-PSEUDOMONAL ACTIVITY

Objective: Efficacy of Aqueous and Methanolic Extracts of Plant Brassica Rapa Leaves Potential Anti-Pseudomanal Activity
 Methods: Phytochemical analysis of Brassica rapa belonging to the family was examined using Aqueous and Methanolic extracts. Methanolic and aqueous extracts of leaves part of the plant were investigated individually for Anti-pseudomanal activity by agar well diffusion method. The susceptibility of three gram-negative bacteria to the aqueous and Methanolic extracts of this plant was measured and the results compared with standard antibiotic Gentamycin. Both the extracts were tested against selected species of Pseudomonas aeruginosa MTCC2295 (G-ve), Pseudomonas fluorescens MTCC2421 (G-ve), and Pseudomonas alcaligenes MTCC7095 (G-ve), to find the inhibitory activities of microbes at the dose level of 50 and 100 µg/ml.
 Results: The phytochemical analysis of Aqueous and Methanolic extracts confirmed the presence of Carbohydrates, Fat, proteins, Vitamins, Minerals, Electrolytes, sugar, soluble and insoluble fiber and amino acids. Methanolic extract of Brassica rapa showed considerably high Anti-pseudomonal activities against all bacteria than Aqueous extract.
 Conclusion: The leaves of Brassica Rapa were found to be rich in sulphur and nitrogen-containing compounds. Brassicarapamethanolic extract has anti-pseudomonal effects against bacteria.
 The current study's range of activity can provide a new plant source for safe anti-pseudomanal drugs.

Antibacterial Activity Test of Ganitri (Elaeocarpus ganitrus Roxb.) Leaf Methanol Extract against Bacteria (Propionibacterium acne)

Ganitri plant (Elaeocarpus ganitrus Roxb.) is one type of plant that can be discovered in several countries, such as Indonesia. This plant has a number of benefits as it contains flavonoid, tannins, phenolics, alkaloids, along with triterpenoids. In addition, that plant has significant role to decrease microbial activities including Staphylococcus aureus, Bacillus cereus, Candida albicans and Penicillium sp. These activities consist of three stages as follows inhibiting nucleic acid synthesis, then followed by hampering cell membrane function and finally decreasing bacterial energy metabolism. The mechanism denatures cell proteins leading an imbalance of molecules, ions and cell lysis as well. Extraction process has been completed by maceration method of 3-kg-wet leaves, a 1-kg-dry simplicial, a 500-g macerated simplicial mixed with 5 L methanol solvent and 33.33 % of simplicial, and 95.08 g of yield extract with 19.01%. Its process generated green color extract having bitter taste and thick form, 0.5% of water, 15.9% of ash, and 0.4% of insoluble acid. Identification of these active ingredients that using positive phytochemical resulted flavonoids. TLC (Thin-layer chromatography) test was used with 0.75 of positive control quercetin and 0.81 of flavonoid extract. Antibacterial activity testing was applied by calculating the inhibitory power of Nutrient Agar with negative control using sterile water. Afterwards, implemented a positive control using clindamycin resulted of several concentration as follows of 10 % (10.6), 20% (10.6), 30% (9.25), 40% (10.12), 50% (9.37) and 100% (38.75).

Influence of genetic and environmental factors on the contents of carotenoids and phenolic acids in red pepper fruits (Capsicum annuum L.)

Red pepper is enriched in antioxidant components, such as carotenoids, phenolic compounds, and vitamins. In this study, we investigated the natural variability in the content of carotenoids and phenolic acids in 11 red pepper cultivars grown in two locations in South Korea during 2016, 2017, and 2018. Seven carotenoids and six phenolic acids, including soluble and insoluble forms, were detected in the red fruit pericarps. The major carotenoids were β-carotene (40%) and capsanthin (20%). The content of insoluble phenolic acids was higher than that of soluble phenolic acids because of the large amount of insoluble p-coumaric acid. The statistical analysis of combined data showed significant differences among varieties, locations, and years for most of the measured components. The results from variance component analysis indicated that the effects of location, year and the interaction of location and year mainly accounted for the variation in carotenoids, whereas variations in phenolic acid content were attributed to year and variety. In addition, the results of principal component analysis and orthogonal partial least-squares discriminant showed that carotenoids were well discriminated by location and year, whereas phenolic acids were distinctively separated only by year. The data from this study could explain the natural variation in the content of carotenoids and phenolic acids in red pepper fruits by genotype and environment.

Inorganic Elements of Mangium Stem (Acacia mangium Willd) from Different Provenanc

This study aimed to investigate the ash content and concentrations of inorganic elements present in the stem of Acacia mangium. The tree samples (24 years) were collected from five different provenances (Sidei, West of Morehead, Daintree, Ellerbeck, and El Arish). Meanwhile, the disc samples were obtained from the trunk of each tree on the lower parts. The samples were collected from four radial positions (bark, sapwood, outer heartwood, inner heartwood), and the ash and insoluble acid contents were determined. Furthermore, the concentration of 5 elements (Ca, Mg, Fe, Mn, K, and Na) was measured by Atomic Absorption Spectrophotometry (AAS). The interaction of provenance and radial position factors affected ash and potassium contents. Also, acid insoluble ash and calcium contents showed a provenance factor effect. The barks obtained from the tree samples contained the highest concentrations of ash content and most of the elements. The wood and bark of El-Arish provenance showed the lowest (0.50~0.72%) and highest levels (4.75%) of ash content respectively. Furthermore, the highest amount of insoluble acid ash (3075 ppm) and calcium (4513 ppm) content was also measured in samples of El-Arish provenance, and radial position factor was a significant source of variation for Ca, Mg, Na, and Mn concentrations. Except Mg, the inner and outer portions of the heartwood mostly showed no significant difference in unprecedented element concentrations. Ash content was positively correlated with Ca in sapwood (r=0.39) and Mn in bark (r=0.54). In addition, moderate correlations were observed between Mg and Ca in heartwood (r=0.63) and bark (r=0.54) tissues. For ash and silica content, the comparatively low concentration on samples from El-Arish provenance are good options to improve wood quality for breeding programs.

Effect Of The Application Of Different Cooking Periods On The Physicochemical Properties And Microbial Safety Of Hot Pepper Sauce

Hot pepper sauce is a frequently used product in most Ghanaian homes, schools, and restaurants, which occasionally serves as condiments. The cooking period during the production process of hot pepper sauce results in physicochemical changes, which affect the quality and safety of the sauce. The study seeks to determine the effect of the application of different cooking times on the physicochemical properties and microbial safety of hot pepper sauce. The cooking periods were 10 minutes, 20 minutes, 30 minutes, and 1 hour for samples A, B, C, and D. Standard physicochemical and microbiological techniques were employed in the various analysis. Sample D (1 hour cooked) recorded the least moisture and ash content, insoluble acid ash, and a statistically significant acid value (P<0.5) to the other samples (A, B, and C). The total viable count assay indicated anaerobic microorganisms in all the samples assessed, with sample D exhibiting very few microbial populations compared to the others. Staphylococcus species were detected in samples A and B only but undetected in samples C and D. Also, total coliform and Clostridium perfringens were unobserved in any of the samples. Generally, sample D had the best overall acceptability rate, with sample A having the least. This study, therefore, supports the patronization of the hour-cooked hot pepper sauce (sample D) with greater quality, longer shelf-life, and safety.

Analysis of Chemical Properties and Antioxidant Activity of Sambiloto (Andrographis paniculata Nees.) Leaf Tea Formula as a Functional Drink in Preventing Coronavirus Diseases and Degenerative Diseases

AIM: The purpose of this study was to develop a tea formula of sambiloto leaves, analyzed the chemical properties (polyphenols, tannins, water, extracts in water, total ash, water soluble ash, water-insoluble ash, insoluble acid ash, and water-soluble ash alkalinity) as well as the antioxidant capacity of the formula. METHODS: This research was divided into two stages. The first stage was the formulation of sambiloto leaves tea and the second stage was the analysis of chemical properties (levels of polyphenols, tannins, water, extracts water, total ash, water soluble ash, water-insoluble ash, insoluble acid ash, and water soluble ash alkalinity) and antioxidant capacity analysis. The analysis was carried out on all of the sambiloto leaves tea formulas to determine the effect of the formulations on chemical properties and antioxidant capacity. This study used Microsoft Excel 2013 in data processing management. RESULTS: The highest extract water was at F4, and the lowest was at F1. Based on the analysis, the formula of sambiloto leaves tea was in the range of 1.026–1.734%. Of all the formulas that have been prepared, sambiloto leaves tea formula F1 was obtained the best chemical and antioxidant properties among all formulas, which were composed of 100% sambiloto leaves, 5% honey, and 45% dates. CONCLUSION: Sambiloto leaves have good antioxidant activity and can be used as a drink in the form of an infusion, such as tea. The result of brewing the sambiloto tea formula has faded green color. The polyphenol content of the sambiloto leaves tea formula still did not meet the Indonesia National Standard (≥5.2%), was 1.5–3.1%.

Quality test and determination flavonoid content of crackers shallot (allium cepa l.) peel flour

Background: Indonesia culinary tastes are obtained from a blend of various spices used, such as shallots (Allium cepa L.). However, the use of shallots (Allium cepa L.) was limited to its meat only, meanwhile the skin is not utilized. Shallot (Allium cepa L.) peel itself contains flavonoids, polyphenols, saponins, terpenoids and alkaloids. One of the utilization of shallot (Allium cepa L.) peel is by innovating it into flour to make crackers. The aims of this study were to determine the physical test and total flavonoids content of crackers from the skin of shallot (Allium cepa L.). Methods: This study used descriptive quantitative method. The observations of crackers quality was conducted in this study are in accordance with the parameters of crackers SNI 2973: 2011 and RSNI 2973: 2018, which include water content tests, acid insoluble ash content tests, organoleptic tests, hedonic tests, qualitative identification and quantitative analysis of flavonoids. Results: The average percentage of water content of crackers was 2%. The average percentage of insoluble acid ash content of crackers was 0.3%. The organoleptic crackers test results have a special shallots aroma, dark brown in color, and taste delicious. The hedonic test results showed respondents liked the aroma, color, and taste of crackers. Crackers from shallot (Allium cepa L.) peel flour contain positive flavonoid compounds. Conclusion: The determination of total flavonoids content in crackers shallot (Allium cepa L.) peel flour was conducted based on AlCl3 method with total flavonoids expressed in QE (Quercetin equivalent) at the maximum wavelength of 435 nm. The result showed that the average content of flavonoid total is 4,5591 mgQE/g extract.

Standardization and phytochemical screening of Centella asiatica, Moringa oleifera L. leaf and Spirulina sp. simplicia

Centella asiatica, Spirulina sp., Moringa oleifera have been used for traditional medicine. Studying the phytochemical constituents helps to reveal the usage of plants used as lead compounds for active pharmaceutical ingredients in commercial purposes. This study aims to determine the standardization and screening of phytochemicals from CA, ME and Spirulina. Standardization of CA, ME and spirulina showed as follows: total water 5.29 ± 1.1%; 5.98 ± 0.006% and 8.64 ± 1.16, total water soluble simplicia: 19.7± 0.57%; 10.97 ± 0.99 and 10.98 ± 1%, total ethanol soluble simplicia: 22.3 ± 0.57%; 15.99 ± 1.73% and 39.92 ± 1.73%, total ash: 23.11 ± 0.56%; 8.15 ± 0.28% and 8.65 ± 0.28%, total ash insoluble acid: 0.42 ± 0.07%; 2.15 ± 0.28% and 0.66 ± 0.28% respectively. Both simplicia of ME and spirulina contained of alkaloid, tannin, saponin, triterpene/steroid, flavonoid and glycoside whereas CA simplicia contained tannin, saponin, triterpene/steroid, flavonoid and glycoside.

Organic biopolymers of venus clams: Collagen-related matrix in the bivalve shells with crossed-lamellar ultrastructure

BackgroundBiochemical studies and spectroscopic techniques have shown that chitin-silk fibroins are common in nacroprismatic bivalve shells. However, the nature of organic biopolymers in the less well studied shell architectures, such as crossed lamellar shells, remain unknown. Here, two venus shells, Callista disrupta and Callista kingii, with crossed lamellar ultrastructure have been studied. MethodsWe employed thermal gravimetric analysis, optical-, confocal- and scanning electron-microscopes, gel-sodium dodecyl sulfate (gel-SDS), FTIR, ultra-performance liquid chromatography and high-performance anion-exchange chromatography system with pulsed amperometric detection to analyse organic macromolecules in the shells. ResultsThermal analysis showed a low concentration of organic macromolecules in C. disrupta (1.38 wt%) and in C. kingii (1.71 wt%). A combination of biochemical protocols, including Calcofluor White staining and FTIR spectroscopic assessment, indicate that amino-polysaccharide chitin together with proteins, are present in the organic scaffolding of the shells. Scanning electron microscope of insoluble acid biopolymer extracts as well as FTIR technique show that the hierarchical structural organizations of organic biopolymers consist collagen-related matrix. Our histochemical fixing and staining techniques reveal many discrete proteins and glycoproteins from soluble organic macromolecules on the gel-SDS. We show here ‘singlet’ and ‘doublet’ glycosaminoglycan bands that are far above 260 kDa. General significance/conclusionsThe presence of collagen matrix in Callista shells shows promise for the new source of biomaterials. Most importantly, the structural organization of the proteinaceous motif is predominantly helical structures and not silk-fibroin unlike in nacreous bivalve shells.