Insertion Process Research Articles

From Mechanical Machining Technology: A New Solution That Integrates Blades to the Implant to Control the Stress to the Peri-Implant Cortical Bone

Background: To prevent excessive compression of the cortical layer, which can lead to marginal bone loss, various companies have introduced specialized drills. However, these drills often lack the necessary precision, as the operator’s hand may neither be stable enough to prevent ovalization and over-widening nor precise enough to maintain coaxial alignment. Therefore, the aim of this study was to develop a device capable of achieving calibrated cortical preparation in terms of both dimension and coaxiality. Methods: A machining technology based on drilling principles was employed to create the device. Results: Nine blades were incorporated between the transmucosal neck and the implant threads, enabling the blades to cut the cortical bone coaxially during the implant insertion process. Conclusions: The primary goal of this study was to develop an implant capable of achieving calibrated cortical bone preparation, ensuring both precise dimensional control and coaxial alignment. This design incorporates integrated blades that allow for controlled cortical decompression, helping to manage radial compressive stresses during implant placement. Although the experimental studies cited were conducted independently of this research, they validate the functional efficacy of this implant design, demonstrating its ability to promote osseointegration and preserve marginal bone. The results suggest that this implant configuration holds the potential for improving clinical outcomes, particularly in cases where bone quality or density poses challenges to implant stability.

Preparation of high strength and rigidity carbon layer on SiO material surface by dynamic CVD method using gas carbon source C2H2

The main issue in the application of silicon-based negative electrode materials is the inevitable volume expansion, leading to negative electrode material fracture, which severely impacts the performance of batteries. This study employed Chemical Vapor Deposition (CVD) (C2H2@SiO), solid-phase coating method (Pitch@SiO), and liquid-phase coating method (RGFQ@SiO) to coat the surface of SiO materials with a dense amorphous carbon structure. Material property analysis revealed that C2H2@SiO has a relatively small specific surface area (1.8 m2 g−1) and surface carbon increment (2.4 %). It exhibited high reversible specific capacity (1563.4 mAh g−1), high initial Coulombic efficiency (81.45 %), and low volume expansion rate (9.3 %). Mechanical performance testing indicated that the surface carbon layer Young's modulus of C2H2@SiO was the highest (35 GPa), suggesting that using the CVD method to obtain a dense carbon layer can enhance the structural strength of the material. Based on the electrochemical-mechanical coupling theory simulation analysis of the stress during the charge-discharge process of electrode materials, the evolution of concentration and stress field during lithium insertion process was obtained, showing that the coating can further improve the electrochemical and mechanical performance of the negative electrode materials effectively. Additionally, the negative electrode sheets fabricated using sample C2H2@SiO were assembled into 18650 cylindrical batteries, exhibiting excellent cycling performance in 1000 cycles at 25 °C with a capacity retention rate of 85.7 %, along with good rate capability and high/low-temperature performance. The conclusions of this study provide certain guidance for the design and optimization of negative electrode materials for battery electrodes.

A novel method for through-thickness reinforcement of laminated composites using discrete micro-polarization-induced fiber injection (DMFI) approach

Conventional through-thickness reinforcement methods for laminated composites, such as Z-pin, encounter issues with in-plane property degradation and complex fabrication processes. To achieve rapid and low-damage reinforcement, a novel approach using short-chopped carbon fibers (SCFs) to form a micron-diameter interlaminate structure has been proposed. This method employs a discrete micro-polarization-induced fiber injection (DMFI) technique, where polarized SCFs are electrostatically oriented and injected at high speeds into pre-formed holes in the laminates. The insertion process of SCFs was thoroughly investigated, with optimal interlaminate conditions determined using high-speed cameras and other equipment. The toughening mechanism of SCFs was explored through various characterization methods, including metallurgical microscopy. This innovative method offers several advantages over the traditional Z-pin reinforced method. Notably, present method eliminates the need for prefabrication of Z-pins and fully leverages the excellent mechanical properties of individual carbon fiber in short length. It provides superior interlaminar mechanical properties, achieving a 392% improvement compared to the control group and a 15% improvement compared to 0.1 mm Z-pin reinforcement at the same insertion volume fraction. Additionally, it has minimal impact on the in-plane properties of the laminates, with only a 3.6% reduction in tensile strength and a 4.1% reduction in compression strength. Furthermore, it is environmentally friendly, allowing for the recycling and reuse of waste SCFs.

Evolution of [formula omitted]-spacing in clay with PEG bulk volume fraction and molecular weight: Theoretical and experimental study

In the pursuit of ensuring the sustainability of the construction sector and fortifying its resilience against climate change, scientists have dedicated significant efforts to explore new classes of innovative, affordable, and technologically advanced materials that exhibit high efficiency, thereby securing the future for generations to come. In this context, we have opted to produce clay-polymer ecosystems through the direct insertion process. The PEG2000/clay and PEG6000/clay composites underwent analysis using X-ray diffraction (XRD) techniques, revealing the evolution of dhklconcerning the PEG bulk volume fraction. The results indicate the presence of two adsorption cycles, each comprising three stages (dilute, two-dimensional semi-dilute, and plateau). Each cycle delineates the incorporation of a polymer layer into the interlayer spaces of clay minerals. To elucidate this adsorption process, we have proposed a model depicting PEG chain conformation in confined spaces, represented as both monolayer and bilayer configurations. The influence of PEG molecular weight is distinctly evident in the dhklevolution across various clay categories. Ultimately, the application of scaling theory to model the experimental results proves to be robust, offering a precise description of the three discussed regimes.

Organic-inorganic coupled Ti3CN MXene achieving the excellent sodium-ion storage performance

Two-dimensional (2D) transition metal carbides/nitrides (MXenes) are attracting considerable attention due to their unique multilayered structure. However, the practical application of MXenes as electrode materials is hampered by sluggish ions diffusion kinetics and serious volume variation during charging and discharging cycles. Herein, the organic-inorganic coupled Ti3CN (Ti3CNNHSA) composites were constructed by anchoring succinic acid (SA) molecules into the interlayer of Ti3CN. The anchored SA molecules contribute pillar effects to increase interlayer spacing and inhibit the volume contraction during Na+ ions extraction processes, as well as strain effects to mitigate volume expansion during Na+ ions insertion processes. Consequently, the Ti3CNNHSA composites not only exhibit the increased interlayer spacing and rapid ions transfer paths but also deliver the enhanced structural stability and maximum utilization of layered structure, realizing extraordinary cycle stability and high rate capability. The Ti3CNNHSA exhibits a superior capacity retention of 95.2% after 400 cycles at 0.1 A g-1. The Ti3CNNHSA//AC sodium-ion capacitor (SIC) also displays a capacity retention of 84.1% after 1200 cycles at 1.0 A g-1 and a high energy density of 76 Wh kg-1 at the power density of 200 W kg-1. Such an organic-inorganic coupled Ti3CNNHSA sheds light on the development of 2D layered materials.

Extracellular vesicle and CRISPR gene therapy: Current applications in Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, and Huntington's disease.

Neurodegenerative diseases are characterized by progressive deterioration of the nervous system. Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), and Huntington's disease (HD) are prominently life-threatening examples of neurodegenerative diseases. The complexity of the pathophysiology in neurodegenerative diseases causes difficulties in diagnosing. Although the drugs temporarily help to correct specific symptoms including memory loss and degeneration, a complete treatment has not been found yet. New therapeutic approaches have been developed to understand and treat the underlying pathogenesis of neurodegenerative diseases. With this purpose, clustered-regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) technology has recently suggested a new treatment option. Editing of the genome is carried out by insertion and deletion processes on DNA. Safe delivery of the CRISPR/Cas system to the targeted cells without affecting surrounding cells is frequently investigated. Extracellular vesicles (EVs), that is exosomes, have recently been used in CRISPR/Cas studies. In this review, CRISPR/Cas and EV approaches used for diagnosis and/or treatment in AD, PD, ALS, and HD are reviewed. CRISPR/Cas and EV technologies, which stand out as new therapeutic approaches, may offer a definitive treatment option in neurodegenerative diseases.

Revealing an Extended Adsorption/Insertion-Filling Sodium Storage Mechanism in Petroleum Coke-Derived Amorphous Carbon.

Amorphous carbon holds great promise as anode material for sodium-ion batteries due to its cost-effectiveness and good performance. However, its sodium storage mechanism, particularly the insertion process and origin of plateau capacity, remains controversial. Here, an extended adsorption/insertion-filling sodium storage mechanism is proposed using petroleum coke-derived amorphous carbon as a multi-microcrystalline model. Combining in situ X-ray diffraction, in situ Raman, theoretical calculations, and neutron scattering, the effective storage form and location of sodium ions in amorphous carbon are revealed. The sodium adsorption at defect sites leads to a high-potential sloping capacity. The sodium insertion process occurs in both the pseudo-graphite phase (d002 > 0.370nm) and graphite-like phase (0.345 ≤ d002 < 0.370nm) rather than the graphite phase, contributing to low-potential sloping capacity. The sodium filling into accessible closed pores forms quasi-metallic sodium clusters, contributing to plateau capacity. The threshold of the effective interlayer spacing for sodium insertion is extended to 0.345nm, breaking the consensus of insertion interlayer threshold and enhancing understanding of closed pore filling. The extended adsorption/insertion-filling mechanism explains the sodium storage behavior of amorphous carbon with different microstructures, providing theoretical guidance for the rational design of high-performance amorphous carbon anodes.

INCLUSÃO DE ESTUDANTES COM DEFICIÊNCIA NOS JOGOS ESCOLARES DO RIO GRANDE DO SUL

The Jogos Escolares do Rio Grande do Sul (JERGS) are a school sporting event that has been part of the cultural itinerary of the state of Rio Grande do Sul since 1970, when it was created at the Secretaria da Educação e Cultura do Rio Grande do Sul (SEC). Currently, the event is aimed at students from the state's public network, at the municipal, state and federal levels. For many years, students with disabilities did not have an effective space in this school event, which, at times, greatly valued the participation of students with the “athlete” profile. Therefore, the research aims to understand the process of insertion and participation of students with disabilities in JERGS. To achieve this, we determined Cultural History as a theoretical and methodological matrix for research. To access the past about the historical conformations of JERGS, information was collected through documentary sources and digital sources. The collected sources were subjected to the document analysis technique, based on the recording, classification, analysis and cross-referencing procedures. After analyzing and interpreting the collected materials, we found that the JERGS began to include children and adolescents with disabilities in an effective and duly regulated manner from the 2017 edition onwards, the year following the Paralympic Games in Rio de Janeiro. The holding of this mega sporting event in brazilian territory may have encouraged the JERGS organizing committee to include sports adapted for students with disabilities in the event's modalities, almost 50 years since its creation in the state. Received: 2024/04/04 Reformulated: 2024/06/03 Accepted: 2024/08/04

Gender differences in the labour market insertion of tourism graduates

This article studies gender differences in labour mobility, employment type, contract type, and sectoral mismatch among tourism graduates in the first 4 years after graduation. We exploit a longitudinal dataset of seven graduation cohorts to ascertain gender differences in their labour market insertion process at the beginning of their careers. We estimate Heckman probit regressions for each outcome variable that deal with self-selection into employment status. Our results show that females are less likely to move outside their region for labour reasons and more likely to work part-time and with temporal contracts. Moreover, females exhibit a 15 per cent higher probability of being horizontally mismatched in their jobs. Importantly, the documented gap remains over time and even widens, suggesting a faster transition of males towards matched jobs and better labour conditions.

Sulfurized Two-Dimensional Conductive Metal-Organic Framework as a High-Performance Cathode Material for Rechargeable Mg Batteries.

Rechargeable Mg batteries are a promising energy storage technology to overcome the limitations inherent to Li ion batteries. A critical challenge in advancing Mg batteries is the lack of suitable cathode materials. In this work, we report a cathode design that incorporates S functionality into two-dimensional metal-organic-frameworks (2D-MOFs). This new cathode material enables good Mg2+ storage capacity and outstanding cyclability. It was found that upon the initial Mg2+ insertion and disinsertion, there is an apparent structural transformation that crumbles the layered 2D framework, leading to amorphization. The resulting material serves as the active material to host Mg2+ through reduction and/or oxidation of S and, to a limited extent, O. The reversible nature of S and O redox chemistry was confirmed by spectroscopic characterizations and validated by density functional calculations. Importantly, during the Mg2+ insertion and disinsertion process, the 2D nature of the framework was maintained, which plays a key role in enabling the high reversibility of the MOF cathode.

Impedance and Functional Outcomes in Robotic-Assisted or Manual Cochlear Implantation: A Comparative Study

Introduction: Preservation of residual hearing, mainly the low frequencies, is the current main objective of cochlear implantation. New electrode arrays and the development of minimally invasive surgery have allowed electroacoustic stimulation. Over the past several years, robotic-assisted cochlear implant surgery aimed to improve the insertion process while respecting inner ear structures. However, the introduction of a foreign body inside the cochlea can lead to the development of fibrous tissue around the electrode array, or even induce osteogenesis. These histological changes disrupt the parameters of the cochlear implant, resulting in elevated impedance. In addition, long-term auditory performance can be affected, with a deterioration in word comprehension. We evaluated the potential impact of RobOtol® on impedance changes over time, leading to potentially positive functional outcomes. Methods: Cochlear implant surgery with a round window approach was performed under general anesthesia. Fifteen Med-El Flex24 electrode arrays were inserted manually and 24 using RobOtol®. All subjects underwent pure-tone audiometry tests before the surgery and at regular intervals up to 1 year after the surgery. Based on the pure-tone average at the low frequencies from 250 to 1,000 Hz, we divided the patients according to the degree of auditory preservation (full preservation ≤15 dB, partial preservation 15 dB–30 dB, significant loss >30 dB). These different groups were compared in terms of impedance changes and auditory performance, specifically word recognition score. Results: We found proportionally fewer patients who experienced significant low-frequency hearing loss after robotic insertion (53.33% in the manual group compared to 41.67% in the robot-assisted insertion group). Impedance changes at the apex of the electrode array, especially at the first electrode (p = 0.04), after robotic surgery, with less overall variability, a continuous decreasing trend without secondary elevation, and lower values in cases of complete residual hearing preservation (for the three first electrodes: p = 0.017, p = 0.04, p = 0.045). The speech intelligibility amelioration over time showed favorable evolution in patients with complete residual hearing preservation regardless of the insertion method. However, in the absence of auditory preservation, the positive evolution continued more than 6 months after robotic surgery but stagnated after manual insertion (difference at 1 year, p = 0.038; median auditory capacity index 83% vs. 57%). Conclusion: Atraumatic electrode array insertion with consistent, slow speed and the assistance of RobOtol® minimizes disturbances in the delicate neurosensory structures of the inner ear and leads to better auditory performance.

Integrated Insights into NH4 + Storage Mechanism and Electrochemical Kinetics of Ultrastable Prussian Blue Analogues

Ammonium-ion batteries (AIBs) have recently attracted increasing attention in the field of aqueous batteries owing to their high safety and fast diffusion kinetics.[1, 2] The NH4 + storage mechanism is quite different from that of spherical metal ions (e.g. Li+, Na+, K+, Mg2+, and Zn2+) because of the formation of hydrogen bonds between NH4 + and host materials. As one of the main host materials, PBAs with 3D channels have been extensively investigated in AIBs. The formula for PBAs can be roughly expressed as AxMm[Mn(CN)6]y·zH2O, where A represents the cation (e.g. Na and K) for charge compensation, Mm and Mn stand for transition-metal ions (e.g. Cu, Ni), respectively. m, n, x, and y are their stoichiometric values. The size of the channels in PBAs is about 0.32 × 0.32 nm, which is larger than the ionic radius of NH4 + (0.154 nm). In this work, the NH4 + storage mechanism during the charging/discharging process is investigated by in-situ XRD and showed stable structure. In addition, the DFT calculation further demonstrates the insertion/extraction mechanism. During the NH4 + insertion process, hydrogen bonds are formed between the hydrogen atom in NH4 + and nitrogen atom in PBAs framework, which is beneficial for electrochemical and structural stability.

Implementing a Compliance Monitoring Process to Promote Chlorhexidine Gluconate Bathing and Hand Hygiene: An Initiative to Decrease Central Line–Associated Bloodstream Infections

Background Chlorhexidine gluconate (CHG) bathing has proven to reduce central line–associated bloodstream infections (CLABSI) in hospitalized patients. The aim of this study is to evaluate whether the implementation of a compliance monitoring process for CHG bathing and strict hand hygiene as part of the traditional CLABSI prevention bundle will reduce the overall hospital CLABSI standardized infection ratio (SIR). Methods A quasi-experimental study was conducted in intensive care and non-intensive care inpatient hospital units in an urban 361-bed teaching hospital in Western Pennsylvania. The pre-intervention period consisted of January–August 2021, the intervention period consisted of September–November 2021, and the post-intervention and sustainability period consisted of December 2021–June 2022. A compliance monitoring process for the initiation of a daily bath using a 4% CHG solution and strict hand hygiene surveillance was implemented as part of the standard CLABSI prevention bundle (which includes standardized insertion checklists and processes, daily necessity assessment, dressing change and integrity standards, five moments hand hygiene standards, CHG disk [Biopatch], end caps [Curos], and care of tubing). Outcomes A statistically significant increase in hand hygiene (p=&lt;0.001) and in CHG bathing compliance (p=0.014) helped reduce the overall hospital CLABSI SIR from 1.45 to 0.82, standing for an overall 43.4% decrease. Cost reduction was statistically significant (p=0.011) and was an estimated $1.4 million dollars in savings for the hospital. Next Steps Incorporate the compliance monitoring component for CHG bathing and hand hygiene as part of the best strategy for CLABSI bundle prevention within the healthcare system.

Transition-Metal-Catalyzed Directed C-H Bond Functionalization with Iodonium Ylides: A Review of the Last 5 Years.

Transition-metal-catalyzed directed C-H functionalization with various carbene precursors has been widely employed for constructing a wide range of complex and diverse active molecules through metal carbene migratory insertion processes. Among various carbene precursors, iodonium ylides serve as a novel and emerging carbene precursor with features including easy accessibility, thermal stability and high activity, which have attracted great attention from organic chemists and have achieved tremendous success in organic transformation. In this review, recent progress on the application of iodonium ylides with multifunctional coupling characteristics in C-H bond activation reactions is summarized, and the potential of iodonium ylides is discussed.

The Pathway of a Transmembrane Helix Insertion into the Membrane Assisted by Sec61α Channel.

The significant inconsistency between the experimental and simulation results of the free energy for the translocon-assisted insertion of the transmembrane helix (TMH) has not been reasonably explained. Understanding the mechanism of TMH insertion through the translocon is the key to solving this problem. In this study, we performed a series of coarse-grained molecular dynamics simulations and calculated the potential mean forces (PMFs) for three insertion processes of a hydrophobic TMH. The simulations reveal the pathway of the TMH insertion assisted by a translocon. The results indicate that the TMH contacts the top of the lateral gate first and then inserts down the lateral gate, which agrees with the sliding model. The TMH begins to transfer laterally to the bilayer when it is blocked by the plug and reaches the exit of the lateral gate, where there is a free energy minimum point. We also found that the connecting section between TM2 and TM3 of Sec61α prevented TMH from leaving the lateral gate and directly transitioning to the surface-bound state. These findings provide insight into the mechanism of the insertion of TMH through the translocon.

Sulfurized Two‐Dimensional Conductive Metal‐Organic Framework as a High‐Performance Cathode Material for Rechargeable Mg Batteries

Rechargeable Mg batteries are a promising energy storage technology to overcome the limitations inherent to Li ion batteries. A critical challenge in advancing Mg batteries is the lack of suitable cathode materials. In this work, we report a cathode design that incorporates S functionality into two‐dimensional metal‐organic‐frameworks (2D‐MOFs). This new cathode material enables good Mg2+ storage capacity and outstanding cyclability. It was found that upon the initial Mg2+ insertion and disinsertion, there is an apparent structural transformation that crumbles the layered 2D framework, leading to amorphization. The resulting material serves as the active material to host Mg2+ through reduction and/or oxidation of S and, to a limited extent, O. The reversible nature of S and O redox chemistry was confirmed by spectroscopic characterizations and validated by density functional calculations. Importantly, during the Mg2+ insertion and disinsertion process, the 2D nature of the framework was maintained, which plays a key role in enabling the high reversibility of the MOF cathode.

A novel framework inspired by human behavior for peg-in-hole assembly

PurposeThe purpose of this paper is to enhance the performance of robots in peg-in-hole assembly tasks, enabling them to swiftly and robustly accomplish the task. It also focuses on the robot’s ability to generalize across assemblies with different hole sizes.Design/methodology/approachHuman behavior in peg-in-hole assembly serves as inspiration, where individuals visually locate the hole firstly and then continuously adjust the peg pose based on force/torque feedback during the insertion process. This paper proposes a novel framework that integrate visual servo and adjustment based on force/torque feedback, the authors use deep neural network (DNN) and image processing techniques to determine the pose of hole, then an incremental learning approach based on a broad learning system (BLS) is used to simulate human learning ability, the number of adjustments required for insertion process is continuously reduced.FindingsThe author conducted experiments on visual servo, adjustment based on force/torque feedback, and the proposed framework. Visual servo inferred the pixel position and orientation of the target hole in only about 0.12 s, and the robot achieved peg insertion with 1–3 adjustments based on force/torque feedback. The success rate for peg-in-hole assembly using the proposed framework was 100%. These results proved the effectiveness of the proposed framework.Originality/valueThis paper proposes a framework for peg-in-hole assembly that combines visual servo and adjustment based on force/torque feedback. The assembly tasks are accomplished using DNN, image processing and BLS. To the best of the authors’ knowledge, no similar methods were found in other people’s work. Therefore, the authors believe that this work is original.

Experimental Study of Needle Insertion into Gerbil Tympanic Membrane.

The perforation characteristics and fracture-related mechanical properties of the tympanic membrane (TM) greatly affect surgical procedures like myringotomy and tympanostomy performed on the middle ear. We analyzed the most important features of the gerbil TM perforation using an experimental approach that was based on force measurement during a 2-cycle needle insertion/extraction process. Fracture energy, friction energy, strain energy, and hysteresis loss were taken into consideration for the analysis of the different stages of needle insertion and extraction. The results demonstrated that (1) although the TM shows viscoelastic behavior, the contribution of hysteresis loss was negligible compared to other irreversible dissipated energy components (i.e., fracture energy and friction energy). (2) The TM puncture force did not substantially change during the first hours after animal death, but interestingly, it increased after 1week due to the drying effects of soft tissue. (3) The needle geometry affected the crack length and the most important features of the force-displacement plot for the needle insertion process (puncture force, puncture displacement, and jump-in force) increased with increasing needle diameter, whereas the insertion velocity only changed the puncture and jump-in forces (both increased with increasing insertion velocity) and did not have a noticeable effect on the puncture displacement. (4) The fracture toughness of the gerbil TM was almost independent of the needle geometry and was found to be around 0.33 0.10kJ/m2.

De Novo Synthesis of α-Ketoamides via Pd/TBD Synergistic Catalysis.

Precisely controlling the product selectivity of a reaction is an important objective in organic synthesis. α-Ketoamides are vital intermediates in chemical transformations and privileged motifs in numerous drugs, natural products, and biologically active molecules. The selective synthesis of α-ketoamides from feedstock chemicals in a safe and operationally simple manner under mild conditions is a long-standing catalysis challenge. Herein, an unprecedented TBD-switched Pd-catalyzed double isocyanide insertion reaction for assembling ketoamides in aqueous DMSO from (hetero)aryl halides and pseudohalides under mild conditions is reported. The effectiveness and utility of this protocol are demonstrated by its diverse substrate scope (93 examples), the ability to late-stage modify pharmaceuticals, scalability to large-scale synthesis, and the synthesis of pharmaceutically active molecules. Mechanistic studies indicate that TBD is a key ligand that modulates the Pd-catalyzed double isocyanide insertion process, thereby selectively providing the desired α-ketoamides in a unique manner. In addition, the imidoylpalladium(II) complex and α-ketoimine amide are successfully isolated and determined by X-ray analysis, confirming that they are probable intermediates in the catalytic pathway.

Leveraging Machine Learning-Guided Molecular Simulations Coupled with Experimental Data to Decipher Membrane Binding Mechanisms of Aminosterols.

Understanding the molecular mechanisms of the interactions between specific compounds and cellular membranes is essential for numerous biotechnological applications, including targeted drug delivery, elucidation of the drug mechanism of action, pathogen identification, and novel antibiotic development. However, estimation of the free energy landscape associated with solute binding to realistic biological systems is still a challenging task. In this work, we leverage the Time-lagged Independent Component Analysis (TICA) in combination with neural networks (NN) through the Deep-TICA approach for determining the free energy associated with the membrane insertion processes of two natural aminosterol compounds, trodusquemine (TRO), and squalamine (SQ). These compounds are particularly noteworthy because they interact with the outer layer of neuron membranes, protecting them from the toxic action of misfolded proteins involved in neurodegenerative disorders, in both their monomeric and oligomeric forms. We demonstrate how this strategy could be used to generate an effective collective variable for describing solute absorption in the membrane and for estimating free energy landscape of translocation via on-the-fly probability enhanced sampling (OPES) method. In this context, the computational protocol allowed an exhaustive characterization of the aminosterol entry pathway into a neuron-like lipid bilayer. Furthermore, it provided accurate prediction of membrane binding affinities, in close agreement with the experimental binding data obtained by using fluorescently labeled aminosterols and large unilamellar vesicles (LUVs). The findings contribute significantly to our understanding of aminosterol entry pathways and aminosterol-lipid membrane interactions. Finally, the computational methods deployed in this study further demonstrate considerable potential for investigating membrane binding processes.