Ecological sensitivity, as one of the most important indicators to evaluate regional environmental issues, holds significant implications for ecological governance and management in the related area. This study utilized remote sensing imagery of Landsat Thematic Mapper (TM) from the Yangtze River Delta (YRD) in 2014 and 2018, combined with field surveys and socio-economic data. Considering the local ecological and environmental conditions in the region, nine factors related to seven aspects, soil erosion, topography, humidity, habitat, water environment, human interference, and climate, were selected to create an ecological sensitivity evaluation framework for the YRD urban agglomeration. The coefficient of variation method was applied to determine factor weights, while the zonal statistics and spatial overlay methods were used for a comprehensive analysis of ecological sensitivity in a geographic information system (GIS). The YRD urban agglomeration was categorized into five ecological sensitivity levels: extremely sensitive, highly sensitive, moderately sensitive, slightly sensitive, and insensitive. The analysis results revealed spatial variations in the distribution of ecological sensitivity across the YRD urban agglomeration, with the overall ecological sensitivity level being slightly sensitive. The proportions of the total area occupied by extremely sensitive, highly sensitive, moderately sensitive, slightly sensitive, and insensitive zones were 14.30%, 12.02%, 25.29%, 30.34%, and 18.05% in 2014, and 14.30%, 24.01%, 16.33%, 27.32%, and 18.05%, respectively, in 2018. Based on these results, relevant ecological vulnerabilities for the YRD urban agglomeration were discussed.